Radish bud seedling cultivation method
Through the combination of nano-carbon sol treatment and hydroponics, the sulforin synthesis pathway of radish sprouts is regulated, which solves the problem of increasing sulforin content in the existing technology, and achieves the synergistic effect of improving the quality of radish sprouts and green production.
Patent Information
- Application Number
- CN202510671474.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-23
- Publication Date
- 2025-07-11
AI Technical Summary
The existing technology is difficult to increase the sulforin content in radish sprouts efficiently, safely and easily. The traditional method has high cost and long cycles, and the regulation of nanocarbon in hydroponic sprouts is limited.
The radish seeds were treated by nanocarbon sol and nanocarbon and potassium salt solutions were added during hydroponics to regulate the thioglycoside synthesis pathway of radish sprouts, and the nanocarbon sol was prepared by double graphite electrode electrolysis, which combined with specific light and humidity conditions to promote thioglycoside synthesis.
Significantly increase the sulfide content in radish sprouts, shorten the growth cycle, is suitable for large-scale production, and improve quality and market competitiveness.
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Figure BDA0005416539890000071 
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of vegetable cultivation, and particularly relates to a method for cultivating radish sprouts. Background Art
[0002] Radish sprouts are delicious and nutritious, rich in protein, calcium, vitamin C, cellulose, etc., which are beneficial to the human body. As a member of the cruciferous family, it has specific glucosinolate components. Glucosinolates, as a class of plant secondary metabolites containing sulfur and nitrogen elements, the enzymatic hydrolysis products not only have antioxidant functions, but also can prevent chronic diseases (including cardiovascular diseases, depression, obesity, etc.), and have important medicinal value in preventing and inhibiting tumors, and the conversion rate of glucosinolate enzymatic hydrolysis products is higher than that of broccoli. Therefore, enhancing the content of glucosinolates in sprouts is of great significance for enhancing its nutritional value and market competitiveness.
[0003] Currently, research on improving the glucosinolate content of sprouts mainly focuses on environmental regulation and exogenous substance induction. For example, by adjusting light conditions (such as specific wavelength LED light quality), optimizing the nutrient solution formula or applying chemical inducers (such as methyl jasmonate, salicylic acid), etc. However, traditional methods have certain limitations. The cost of light regulation equipment is high and the energy consumption is large. The methods for improving the glucosinolate content by gene editing or breeding in the prior art have a long cycle and high technical threshold, and it is difficult to apply to the large-scale production of short-cycle crops such as sprouts. For example, patent publication number CN109169229A discloses a method for increasing the content of isothiocyanates in radish sprouts. The radish sprouts have a hypocotyl length of 11.0 - 11.5 cm, a fresh weight of 2.1 - 2.4 g, a root length of 7.5 - 7.9 cm, and a total isothiocyanate content of 41 - 110 μmol / g. The method includes treating and germinating radish seeds; for the treatment, the radish seeds are placed in an aqueous sulfate solution at 28 - 30 °C, soaked for 10 - 12 hours, and then germinated.
[0004] In recent years, the application of nanomaterials in the agricultural field has been gradually expanded, and their unique physical and chemical properties provide new ideas for improving crop quality. Nano-carbon materials, due to their high specific surface area, strong adsorption and good biocompatibility, can promote the absorption of nutrients by plant roots, enhance enzyme activity and regulate metabolic pathways. Existing research has shown that nano-carbon treatment can improve the stress resistance and secondary metabolite accumulation of some crops, such as increasing the content of polyphenols in leafy vegetables. However, the research on applying nano-carbon to the hydroponic sprout system in the prior art is extremely limited, and there is no relevant research on its regulation of glucosinolate content.
[0005] Based on this, an efficient, safe and easy-to-operate method is developed, that is, by regulating the glucosinolate synthesis pathway in radish sprouts through nano-carbon treatment, breaking through the bottleneck of existing technologies, realizing the coordination of quality improvement and green production, and providing a solution for the standardized production of functional sprouts. Summary of the Invention
[0006] In view of the above technical problems, the present invention provides a cultivation method for radish sprouts, that is, by regulating the glucosinolate synthesis pathway in radish sprouts through nano-carbon treatment, breaking through the bottleneck of existing technologies, realizing the coordination of quality improvement and green production, and providing a solution for the standardized production of functional sprouts.
[0007] To achieve the above object, the technical solution of the present invention is realized as follows:
[0008] A cultivation method for radish sprouts includes the following steps:
[0009] (1) Pre-treat radish flower seeds by soaking them in a nano-carbon sol solution;
[0010] (2) Cultivate the pre-treated radish flower seeds in a seedling tray until the roots grow out;
[0011] (3) Transfer the radish flower seeds with grown roots to a nutrient solution containing nano-carbon for cultivation.
[0012] In the step (1), the concentration of nano-carbon in the nano-carbon sol solution is 25-200 mg / L.
[0013] Preferably, in the step (1), the concentration of nano-carbon in the nano-carbon sol solution is 50-200 mg / L.
[0014] The time for the pre-treatment is 5-20 h.
[0015] Preferably, the time for the pre-treatment is 15 h.
[0016] The nano-carbon sol is prepared by the double graphite electrode electrolysis method.
[0017] The cultivation method in the step (2) is as follows: lay a layer of wet paper towel in the seedling tray, sow the pre-treated radish flower seeds on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 25-30 °C, a humidity of 75-85%, without light, and spray clear water once every 8-12 h until the roots grow out.
[0018] Preferably, the cultivation method in the step (2) is as follows: lay a layer of wet paper towel in the seedling tray, sow the pre-treated radish flower seeds on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h until the roots grow out.
[0019] The concentration of nano-carbon in the nutrient solution is 25-200 mg / L.
[0020] Preferably, the concentration of nano-carbon in the nutrient solution is 50-200 mg / L.
[0021] The nutrient solution is a plant culture solution; the plant culture solution is a Holland culture solution.
[0022] Potassium salt is also added to the nutrient solution; the concentration of potassium salt in the nutrient solution is 5 mM / L.
[0023] The potassium salt is K2SO4 or / and KNO3.
[0024] The cultivation method in the step (3) is as follows: the cultivation temperature is 25-30 °C, the humidity is 75-85%, the light / dark alternation time is 16 / 8 h, different concentrations of nano-carbon, Holland culture solution and 5 mM / L K2SO4 / KNO3 solution are added to the cultivation tray until it reaches the tray mesh, ensuring that the seedlings with growing roots can absorb the nutrient solution, and the nutrient solution is added to the cultivation tray every 16-24 hours during the day.
[0025] Preferably, the cultivation method in the step (3) is as follows: the cultivation temperature is 28 °C, the humidity is 80%, the light / dark alternation time is 16 / 8 h, different concentrations of nano-carbon, Holland culture solution and 5 mM / L K2SO4 / KNO3 solution are added to the cultivation tray until it reaches the tray mesh, ensuring that the seedlings with growing roots can absorb the nutrient solution, and the nutrient solution is added to the cultivation tray every 24 hours during the day.
[0026] The beneficial effects of the present invention: The method for improving the quality of radish sprouts in the present invention is to add nano-carbon sol that can promote plant growth under normal hydroponic conditions. At an appropriate concentration, nano-carbon increases the content of glucosinolate, an active substance in radish sprouts. This method is simple to operate and suitable for large-scale popularization and application. The specific implementation manners are described in detail below in combination with the following examples. A certain concentration of carbon nanomaterials affects the biosynthesis of glucosinolates in plants, mainly by promoting the absorption of nitrogen and sulfur elements required for glucosinolate synthesis and upregulating the expression of glucosinolate synthesis genes. The pretreatment stage uses nano-carbon sol treatment to promote the rapid germination of seeds and shorten the growth cycle of radish sprouts. Specific implementation manners
[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.
[0028] Example 1
[0029] A cultivation method for radish sprouts, the steps are as follows:
[0030] (1) Weigh 30 g of radish seeds, add a nano-carbon sol dispersion solution of 25 mg / L and soak for 15 h;
[0031] (2) Lay a layer of wet paper towels in the seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0032] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add Holland culture solution containing 25 mg / L of nano-carbon and 5 mM / L of K2SO4 / KNO3 to the cultivation tray up to the tray mesh holes to ensure that the seedlings with grown roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray every 24 h during the day;
[0033] (4) Pick and harvest.
[0034] Example 2
[0035] A cultivation method for radish sprouts, the steps are as follows:
[0036] (1) Weigh 30 g of radish seeds, add a nano-carbon sol dispersion solution of 50 mg / L and soak for 15 h;
[0037] (2) Lay a layer of wet paper towels in the seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0038] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add 50 mg / L of nano-carbon sol, Holland culture solution and 5 mM / L of K2SO4 / KNO3 solution to the cultivation tray up to the tray mesh holes to ensure that the seedlings with grown roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray every 24 h during the day;
[0039] (4) Pick and harvest.
[0040] Example 3
[0041] A cultivation method for radish sprouts, the steps are as follows:
[0042] (1) Weigh 30 g of radish seeds and add 15 h of a 100 mg / L nano-carbon sol dispersion;
[0043] (2) Lay a layer of wet paper towels in a seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, no light, and spray clear water once every 12 h;
[0044] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add a 100 mg / L nano-carbon sol, Holand culture solution, and a 5 mM / L K2SO4 / KNO3 solution to the tray mesh in the cultivation tray to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0045] (4) Harvest by picking.
[0046] Example 4
[0047] A method for cultivating radish sprouts, the steps are as follows:
[0048] (1) Weigh 30 g of radish seeds and soak them in a 200 mg / L nano-carbon sol dispersion for 15 h;
[0049] (2) Lay a layer of wet paper towels in a seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, no light, and spray clear water once every 12 h;
[0050] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add a 200 mg / L nano-carbon sol, Holand culture solution, and a 5 mM / L K2SO4 / KNO3 solution to the tray mesh in the cultivation tray to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0051] (4) Harvest by picking.
[0052] Example 5
[0053] A method for cultivating radish sprouts, the steps are as follows:
[0054] (1) Weigh 30 g of radish seeds and soak them in a 25 mg / L nano-carbon sol dispersion for 15 h;
[0055] (2) Lay a layer of wet paper towels in the seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, and then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0056] (3) After culturing for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light time to 12 h, and the rest of the time to be in the dark. Add 50 mg / L of nano-carbon sol, Holand culture solution and 5 mM / L of K2SO4 / KNO3 solution to the tray mesh to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0057] (4) Pick and harvest.
[0058] Example 6
[0059] A cultivation method for radish sprouts is as follows:
[0060] (1) Weigh 30 g of radish seeds and soak them in a nano-carbon sol dispersion solution with a concentration of 25 mg / L for 15 h;
[0061] (2) Lay a layer of wet paper towels in the seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, and then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0062] (3) After culturing for 48 h until the roots grow out, remove the wet paper towels, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add 200 mg / L of nano-carbon sol, Holand culture solution and 10 mM / L of K2SO4 / KNO3 solution to the tray mesh to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0063] (4) Pick and harvest.
[0064] Example 7
[0065] A cultivation method for radish sprouts is as follows:
[0066] (1) Weigh 30 g of radish seeds and soak them in a nano-carbon sol dispersion solution with a concentration of 50 mg / L for 15 h;
[0067] (2) Lay a layer of wet paper towels in the seedling tray, sow the seeds obtained in step (1) on the wet paper towels in the cultivation tray, and then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0068] (3) After culturing for 48 h until the roots grow out, remove the wet paper towel, set the culturing temperature at 28 °C, the humidity at 80%, the light / dark alternating treatment time at 16 / 8 h, add 25 mg / L of nano-carbon sol, Holand culture solution and 5 mM / L of K2SO4 / KNO3 solution to the tray mesh in the culture dish to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culture dish every 24 h during the day;
[0069] (4) Harvest by picking.
[0070] Example 8
[0071] A cultivation method for radish sprouts, the steps are as follows:
[0072] (1) Weigh 30 g of radish seeds, add a dispersion solution of 200 mg / L of nano-carbon sol and soak for 18 h;
[0073] (2) Lay a layer of wet paper towel in the seedling cultivation tray, sow the seeds obtained in step (1) on the wet paper towel in the culture dish, then place the seedling cultivation tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0074] (3) After culturing for 48 h until the roots grow out, remove the wet paper towel, set the culturing temperature at 28 °C, the humidity at 80%, the light time at 12 h, and the rest of the time in dark treatment, add 25 mg / L of nano-carbon sol, Holand culture solution and 5 mM / L of K2SO4 / KNO3 solution to the tray mesh in the culture dish to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culture dish every 24 h during the day;
[0075] (4) Harvest by picking.
[0076] Comparative Example 1
[0077] A cultivation method for radish sprouts, the steps are as follows:
[0078] (1) Weigh 30 g of radish seeds and soak them in clear water for 15 h;
[0079] (2) Lay a layer of wet paper towel in the seedling cultivation tray, sow the seeds obtained in step (1) on the wet paper towel in the culture dish, then place the seedling cultivation tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0080] (3) After culturing for 48 h until the roots grow out, remove the wet paper towel, set the culturing temperature at 28 °C, the humidity at 80%, the light / dark alternating treatment time at 16 / 8 h, add Holand culture solution to the culturing tray up to the mesh of the tray, ensuring that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culturing tray every 24 h during the day;
[0081] (4) Harvest by picking.
[0082] Comparative Example 2
[0083] A method for culturing radish sprouts, the steps are as follows:
[0084] (1) Weigh 30 g of radish seeds and soak them in clear water for 15 h;
[0085] (2) Lay a layer of wet paper towel in the seedling-raising tray, sow the seeds obtained in step (1) on the wet paper towel in the culturing tray, then place the seedling-raising tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0086] (3) After culturing for 48 h until the roots grow out, remove the wet paper towel, set the culturing temperature at 28 °C, the humidity at 80%, the light / dark alternating treatment time at 16 / 8 h, add Holand culture solution and 5 mM / L K2SO4 / KNO3 solution to the culturing tray up to the mesh of the tray, ensuring that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culturing tray every 24 h during the day;
[0087] (4) Harvest by picking.
[0088] Comparative Example 3
[0089] A method for improving the quality of radish sprouts, the steps are as follows:
[0090] (1) Weigh 30 g of radish seeds and soak them in 50 mg / L nano-carbon sol dispersion for 15 h;
[0091] (2) Lay a layer of wet paper towel in the seedling-raising tray, sow the seeds obtained in step (1) on the wet paper towel in the culturing tray, then place the seedling-raising tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0092] (3) After culturing for 48 h until the roots grow out, remove the wet paper towel, set the culturing temperature at 28 °C, the humidity at 80%, the light / dark alternating treatment time at 16 / 8 h, add Holand culture solution and 5 mM / L K2SO4 / KNO3 solution to the culturing tray up to the mesh of the tray, ensuring that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culturing tray every 24 h during the day;
[0093] (4) Harvest by picking.
[0094] Comparative Example 4
[0095] A method for improving the quality of radish sprouts, the steps are as follows:
[0096] (1) Weigh 30 g of radish seeds and soak them in clear water for 15 h;
[0097] (2) Lay a layer of wet paper towel in the seedling tray, sow the seeds obtained in step (1) on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28°C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0098] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towel, set the cultivation temperature to 28°C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add 50 mg / L of nano-carbon sol, Holand culture solution and 5 mM / L of K2SO4 / KNO3 solution to the tray mesh in the cultivation tray to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0099] (4) Harvest by picking.
[0100] Measure the glucosinolate content and amino acid content of the harvested radish sprouts in different test groups, and the results are shown in Table 1.
[0101] Among them, in Comparative Example 1, other methods are the same as those in the example, the only difference is that nano-carbon is not used in the pretreatment stage, and K2SO4 / KNO3 and nano-carbon are not used in the hydroponic stage; Comparative Example 2: other methods are the same as those in the example, the only difference is that nano-carbon is not used in the pretreatment and hydroponic stages; in Comparative Example 3, other methods are the same as those in the example, the difference is that nano-carbon is not used in the hydroponic stage; in Comparative Example 4, other methods are the same as those in the example, the difference is that nano-carbon is not used in the pretreatment stage.
[0102] As can be seen from the content recorded in Table 1, the data of Example 2 are the best, significantly higher than those of other comparative examples, indicating that the method of the present invention can significantly increase the glucosinolate content in radish sprouts and improve the quality of radish sprouts.
[0103] Table 1 Main glucosinolate monomers and total glucosinolate content in radish sprouts
[0104]
[0105]
[0106] Note: GRH, glucoraphasatin (4-methylthio-3-butenyl glucosinolate); GRE, glucoraphenin (4-methylsulfinyl-3-butenyl glucosinolate); GIB, glucoiberin (3-methylsulfinyl glucosinolate); 4MGBS, 4-methoxyglucobrassicin (4-hydroxy-3-indolylmethyl glucosinolate); 4HGBS, 4-hydroxyglucobrassicin (4-methoxy-3-indolylmethyl glucosinolate).
[0107] Example 9
[0108] A method for cultivating radish sprouts, the steps are as follows:
[0109] (1) Weigh 30 g of radish seeds and add 50 mg / L of nano-carbon sol dispersion for 5 h;
[0110] (2) Lay a layer of wet paper towel in the seedling tray, sow the seeds obtained in step (1) on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0111] (3) After cultivating for 48 h until the roots grow out, remove the wet paper towel, set the cultivation temperature to 28 °C, the humidity to 80%, the light / dark alternating treatment time to 16 / 8 h, add 50 mg / L of nano-carbon sol, Holland culture solution and 10 mM / L of K2SO4 / KNO3 solution to the tray mesh to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the cultivation tray once every 24 h during the day;
[0112] (4) Harvest by picking.
[0113] Example 10
[0114] A method for cultivating radish sprouts, the steps are as follows:
[0115] (1) Weigh 30 g of radish seeds and add 200 mg / L of nano-carbon sol dispersion for 20 h;
[0116] (2) Lay a layer of wet paper towel in the seedling tray, sow the seeds obtained in step (1) on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 28 °C, a humidity of 80%, without light, and spray clear water once every 12 h;
[0117] (3) After culturing for 48 h until the roots grow out, remove the wet tissue paper, set the culturing temperature at 28 °C, the humidity at 80%, the light / dark alternating treatment time at 16 / 8 h, add nano-carbon sol at 200 mg / L, Holand culture solution and 1 mM / L K2SO4 / KNO3 solution to the tray mesh in the culture dish to ensure that the seedlings with growing roots can absorb the nutrient solution, and add the nutrient solution to the culture dish every 24 h during the day;
[0118] (4) Harvest.
[0119] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A cultivation method of radish sprouts, characterized in that, It includes the following steps: (1) Pre-treat the radish flower seeds by soaking them in a nano-carbon sol solution; (2) Cultivate the pre-treated radish flower seeds in a seedling tray until the roots grow out; (3) Transfer the radish flower seeds with grown roots to a nutrient solution containing nano-carbon for cultivation.
2. The cultivation method of radish sprouts according to claim 1, characterized in that, In step (1), the concentration of nano-carbon in the nano-carbon sol solution is 25 - 200 mg / L.
3. The cultivation method of radish sprouts according to claim 2, characterized in that, In step (1), the concentration of nano-carbon in the nano-carbon sol solution is 50 - 200 mg / L.
4. The cultivation method of radish sprouts according to claim 1, characterized in that, The time for the pre-treatment is 5 - 20 h.
5. The cultivation method of radish sprouts according to claim 4, characterized in that, The cultivation method in step (2) is as follows: Lay a layer of wet paper towel in the seedling tray, sow the pre-treated radish flower seeds on the wet paper towel in the cultivation tray, then place the seedling tray in a plant incubator at a temperature of 25 - 30 °C, a humidity of 75 - 85%, without light, spray clear water once every 8 - 12 h, and wait until the roots grow out.
6. The cultivation method of radish sprouts according to claim 1, characterized in that, The concentration of nano-carbon in the nutrient solution is 25 - 200 mg / L.
7. The cultivation method of radish sprouts according to claim 6, characterized in that, The concentration of nano-carbon in the nutrient solution is 50 - 200 mg / L.
8. The cultivation method of radish sprouts according to claim 1, characterized in that, The nutrient solution is a plant culture solution; the plant culture solution is Holand culture solution.
9. The cultivation method of radish sprouts according to any one of claims 1-8, characterized in that, Potassium salt is also added to the nutrient solution; the concentration of potassium salt in the nutrient solution is 1 - 10 mM / L.
10. The cultivation method of radish sprouts according to claim 9, characterized in that, The potassium salt is K2SO4 or / and KNO3.