Pine long-horned beetle attractant as well as preparation method and application thereof

Abstract

The attractant is composed of (1R)-2, 2-dimethyl-3-methylene dicycloheptane, pinene, camphene and other components, 95% ethyl alcohol is used as a solvent, porous silica gel microspheres with the average pore size of 2-5 nm are matched to serve as a slow-release carrier, and the pine tree longicorn attractant is prepared. The preparation method comprises the steps of step-by-step mixing, ultrasonic dispersion, standing reaction and slow-release packaging, and process parameters of each step are accurately controlled. Compared with the prior art, the pine tree longhorn beetle attractant has the remarkable advantages that through precise matching of the components and collaborative optimization of technological parameters, triple breakthrough of the pine tree longhorn beetle attractant in targeting, stability and cost control is achieved, and an efficient and reliable technical scheme is provided for green prevention and control of pine wood nematode diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of forest pest control, and particularly to a pine sawyer attractant and its preparation method and application. Background Art

[0002] As the main vector of pine wilt disease, the feeding behavior of adult pine sawyers causes damage to the tender branches and bark of pine trees, and the boring of larvae results in the necrosis of the xylem, ultimately causing the death of host plants. The current mainstream control technology mainly relies on the pheromone trapping method. However, the existing technical solutions still have limitations: Chinese Patent No. CN114586781A discloses a tea longicorn attractant composition, attractant and its preparation method and application, with linalool and geraniol as the core components, but the allelopathic signal has a low matching degree with the biological characteristics of pine sawyers and lacks sufficient specificity for pine sawyers. Chinese Patent No. CN118901707A adopts a compound system of 2-undecyloxy-1-ethanol and β-pinene. Although it has a trapping effect on Ips typographus, the production cost per unit dose of the synthetic pheromone 2-undecyloxy-1-ethanol is high, and the volatile half-life of β-pinene is only 7-10 days at 25°C, resulting in a short effective action period of the lure core and the need for frequent maintenance. Chinese Patent No. CN119563618A integrates plant-derived pheromones such as β-pinene and ethanol with the insect aggregation pheromone 2-(undecyloxy)-1-ethanol, but does not contain the characteristic volatiles released by pine trees after being damaged by pests, and does not solve the stability problem of the co-volatile of multiple components, resulting in large fluctuations in trapping efficiency.

[0003] In addition, the existing attractants generally have the following defects: (1) Poor synergistic effect and slow-release performance: Single components or simple compounding lead to a low matching degree between the volatile spectrum and the olfactory receptors of the antennae of sawyers, and the lack of effective stabilizers and slow-release carriers makes them vulnerable to the environment; (2) Insufficient component specificity: The existing attractants do not fully simulate the "distress signal" released by pine trees after being attacked by sawyers, affecting the recognition efficiency of the chemosensors of sawyers; (3) High environmental risk and cost: Some technologies rely on synthetic pheromones or complex processes, posing a risk of toxicity to non-target organisms, such as the risk of contact killing of bees, and the preparation cost is high, making them unsuitable for large-scale promotion.

[0004] The disclosure of the above background art content is only used to assist in understanding the inventive concept and technical solution of the present invention, and it does not necessarily belong to the prior art of this patent application. Without clear evidence indicating that the above content was publicly available on the filing date of this patent application, the above background art should not be used to evaluate the novelty and inventiveness of this application. Summary of the Invention

[0005] The object of the present invention is to provide a pine sawyer attractant and its preparation method and application to solve the technical problems existing in the above prior art.

[0006] To this end, the present invention adopts the following technical solutions:

[0007] A pine sawyer attractant, comprising an attractant and a slow-release carrier, the slow-release carrier being used for adsorbing the attractant; wherein the attractant comprises the following components in mass percentage: (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane: 5%-10%, pinene: 10%-20%, camphene: 5%-10%, ocimene: 10%-20%, longifolene: 10%-20%, (+)-limonene: 10%-20%, synergist: 1%-3%, stabilizer: 0.5%-2%, and the balance is made up to 100% with 95% ethanol.

[0008] Furthermore, the pinene is a mixture of α-pinene and β-pinene, and the volume ratio of α-pinene to β-pinene is 1:2.8-3.2.

[0009] Furthermore, the synergist is γ-terpinene.

[0010] Furthermore, the stabilizer is geranyl propionate.

[0011] Furthermore, the slow-release carrier is a porous silica microsphere, and the average pore diameter of the porous silica microsphere is 2-5 nm.

[0012] A preparation method of a pine sawyer attractant, comprising the following steps:

[0013] S1. Step-by-step mixing: Heating 95% ethanol to 35-40 °C, and successively adding (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, pinene, and camphene, and stirring until evenly mixed;

[0014] S2. Ultrasonic dispersion: Adding ocimene, longifolene, and (+)-limonene, and performing ultrasonic dispersion for 15-25 min;

[0015] S3. Static reaction: Adding the synergist and the stabilizer, and performing a static reaction at 25 °C for 0.8-1.25 h;

[0016] S4. Slow-release encapsulation: Mixing the mixed solution and the porous silica microsphere according to a mass ratio of 1:0.1, and preparing the attractant after adsorption.

[0017] Furthermore, the stirring frequency in step S1 is 100-200 rpm, and the time is 15-25 min; the microwave power in step S2 is 150-250 W.

[0018] The present invention also provides an application of the pine sawyer attractant as described above or the pine sawyer attractant prepared by the preparation method as described above in the preparation of a product for trapping and killing pine sawyers.

[0019] Furthermore, the application method is as follows:

[0020] (1) Encapsulate the prepared pine sawyer attractant in a slow-release device to obtain an attracting device;

[0021] (2) Hang or place the attracting device in a sawyer trap to prepare a product for trapping and killing pine sawyers.

[0022] Furthermore, the slow-release device has a double-layer structure. The inner layer is a polyethylene slow-release bag with a wall thickness of 0.05 - 0.1 mm; the outer layer is a polypropylene protective shell with a wall thickness of 0.4 - 0.8 mm; the attractant is placed in the polyethylene slow-release bag; the attracting device releases the attractant to trap pine sawyers into the sawyer trap to be captured. The hanging density of the sawyer trap is 3 - 5 per mu, the hanging height is 0.8 - 1.2 m, and the distance between the sawyer traps is 150 - 200 m.

[0023] The following is an explanation of the technical principle from the perspectives of the roles and synergistic mechanisms of raw materials and the necessity of preparation process parameters:

[0024] 1. Roles and synergistic mechanisms of raw materials:

[0025] (1R)-2,2-Dimethyl-3-methylenebicyclo[2.2.1]heptane: As a key precursor for the synthesis of pine terpenoids, its release amount is positively correlated with the degree of feeding damage of sawyers to pine trees. When pine trees are invaded by sawyers, this substance will be released, thus triggering the aggregation behavior of sawyers. It is an important signal substance for attracting sawyers to identify damaged host plants.

[0026] Pinene: It is composed of α-pinene and β-pinene mixed in a volume ratio of 1:2.8 - 3.2. β-pinene is a marker volatile of pine trees and one of the key signals for sawyers to identify host pine trees; α-pinene can enhance the spatial diffusion ability of β-pinene in the air. The two work together to form an important chemical signal system for sawyers to identify host plants.

[0027] Camphene: It can regulate the evaporation rate of other volatiles, especially extend the existence time of β-pinene in the gas phase, thus forming a continuous and stable attracting gradient, enabling sawyers to receive attracting signals within a longer time and a wider range.

[0028] Ocimene: It can attract adult sawyers to fly directionally. When it acts synergistically with longifolene, it can enhance the electroantennogram (EAG) response intensity of sawyers, which means it can more effectively stimulate the olfactory system of sawyers and guide sawyers to fly towards the attracting source.

[0029] Longifolene: As a secondary metabolite of pine trees, it has the behavior of inhibiting sawyer oviposition. When sawyers sense the presence of longifolene, they will be forced to gather towards the lure core to find more suitable mates and oviposition sites, thus increasing the probability of sawyers being trapped.

[0030] (+)-Terpinolene: It is a specific volatile of pine needles. It forms a "double-signal anchoring" mechanism with (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane. (+)-Terpinolene can specifically bind to the olfactory receptor neurons (ORNs) of the longhorn beetle antennae, enhancing the directional flight behavior of the longhorn beetle towards pine hosts. When acting synergistically with (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, it can significantly increase the amplitude of the electroantennogram response of the longhorn beetle, greatly improving the specificity of attraction.

[0031] Synergist: It can reduce the intermolecular force of volatile substances, increase the diffusion rate of volatiles in the air, effectively expand the action radius of the attractant, and enable longhorn beetles at a farther distance to perceive the attraction signal.

[0032] Stabilizer: It can form a molecular protective film to inhibit the degradation of volatiles by environmental factors such as ultraviolet light. Geranyl propionate forms a hydrogen bond complex with ethanol through the ester group, inhibiting the photooxidation reaction and ensuring the effectiveness of the attractant for a long time.

[0033] Solvent (95% ethanol): Ethanol can not only dissolve various natural volatiles but also regulate the overall volatility. After mixing with water, it forms a stable nano-dispersion system, which helps various components to be evenly dispersed and volatilized, enabling the attractant to continuously and stably release the attraction signal.

[0034] 2. Necessity of preparation process parameters

[0035] S1 Stepwise mixing: Heat 95% ethanol to 35 - 40 °C. This temperature range is lower than the boiling points of various volatiles, which can not only ensure the full dissolution of components such as (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, pinene, and camphene but also avoid significant volatilization loss of volatiles due to excessive temperature. Stir at a stirring frequency of 100 - 200 rpm for 15 - 25 min. Such stirring parameters can make the components fully mixed evenly, avoid layering, and provide a good basis for subsequent ultrasonic dispersion and reaction.

[0036] S2 Ultrasonic dispersion: After adding ocimene, longifolene, and (+)-terpinolene, perform ultrasonic dispersion at a microwave power of 150 - 250 W for 15 - 25 min. The cavitation effect of ultrasound can break the molecular aggregates of these components, reducing the droplet size from a larger size to below 5 μm, greatly improving the uniformity of the components. This helps the components to volatilize more evenly in subsequent use, forming a stable attraction gradient.

[0037] S3 Static reaction: React statically at 25 °C for 0.8 - 1.25 h. During this process, the synergist and stabilizer react with other volatile substances to form structures such as hydrogen bond complexes. This can reduce the vapor pressure of the volatile substances, achieve pre-stabilization before slow release, reduce the rapid volatilization of volatile substances in the early stage, and extend the effective action time of the attractant.

[0038] S4 Slow-release encapsulation: Mix the mixed solution with porous silica microspheres with an average pore size of 2 - 5 nm at a mass ratio of 1:0.1. Silica microspheres with this pore size have a high specific surface area, which can provide sufficient adsorption sites during the adsorption process, enabling the loading amount of each microsphere to reach an appropriate level. During the adsorption process, the microspheres can effectively encapsulate and slowly release the volatile substances, making the daily release amount of the active ingredient stable within a certain range, ensuring that the attractant continuously plays a role for a long time.

[0039] The beneficial effects of the present invention compared with the prior art include:

[0040] 1. High efficiency in attracting and strong targeting: Through the synergistic effect of multiple core attractant components, it precisely simulates the chemical signals released after pine trees are damaged by longhorn beetles, and can efficiently attract pine sawyers. Compared with the prior art, the trapping amount of pine sawyer adults is significantly increased, and the target pests can be trapped more precisely, while the trapping amount of non-target organisms is greatly reduced, effectively improving the specificity of trapping.

[0041] 2. Long-term stability and strong anti-environmental interference ability: Due to the use of stabilizers, slow-release carriers and double-layer slow-release structures, the attractant of the present invention has good stability and long-term performance, and strong anti-interference ability. The stabilizer can effectively inhibit the degradation of volatile substances, the slow-release carrier can control the release rate of volatile substances, and the double-layer slow-release structure resists rain erosion and photodegradation, is suitable for complex forest environments, and greatly reduces the cost and workload of manually replacing the lure core.

[0042] 3. Environmentally friendly and low cost: The core components of the attractant of the present invention are all natural volatiles, and the proportion of natural components is ≥95%, which is environmentally friendly and has extremely low toxicity to non-target organisms. At the same time, most of the core raw materials are deep processing products of turpentine, with wide sources and low costs. The preparation process is simple, without complex conditions such as high temperature and high pressure, and is suitable for large-scale popularization and application. Specific implementation mode

[0043] In the embodiments of the specific implementation manners of the present invention, the pine sawyer attractant includes an attractant and a slow-release carrier, and the slow-release carrier is used to adsorb the attractant; wherein the attractant includes the following components in mass percentage: (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane: 5%-10%, pinene: 10%-20%, camphene: 5%-10%, ocimene: 10%-20%, longifolene: 10%-20%, (+)-limonene: 10%-20%, synergist: 1%-3%, stabilizer: 0.5%-2%, and the balance is made up to 100% with 95% ethanol.

[0044] The pinene is a mixture of α-pinene and β-pinene, and the volume ratio of α-pinene to β-pinene is 1:2.8-3.2.

[0045] The synergist is γ-terpinene.

[0046] The stabilizer is geranyl propionate.

[0047] The slow-release carrier is a porous silica microsphere, and the average pore diameter of the porous silica microsphere is 2-5 nm.

[0048] The preparation method of the pine sawyer attractant adopted in the embodiment includes the following steps:

[0049] S1. Step-by-step mixing: Heat 95% ethanol to 35-40 °C, and sequentially add (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, pinene, and camphene, and stir until evenly mixed;

[0050] S2. Ultrasonic dispersion: Add ocimene, longifolene, and (+)-limonene, and perform ultrasonic dispersion for 15-25 min;

[0051] S3. Static reaction: Add the synergist and the stabilizer, and perform a static reaction at 25 °C for 0.8-1.25 h;

[0052] S4. Slow-release encapsulation: Mix the mixed solution and the porous silica microsphere according to a mass ratio of 1:0.1, and prepare the attractant after adsorption.

[0053] The stirring frequency in step S1 is 100-200 rpm, and the time is 15-25 min; the microwave power in step S2 is 150-250 W.

[0054] The application method of the pine sawyer attractant prepared in the embodiment in the preparation of a product for trapping and killing pine sawyers is as follows:

[0055] (1) Encapsulate the prepared pine sawyer attractant in a slow-release device to obtain an attracting device;

[0056] (2) Hang or place the attracting device in a sawyer trap to prepare a product for trapping and killing pine sawyers.

[0057] The slow-release device has a double-layer structure. The inner layer is a polyethylene slow-release bag with a wall thickness of 0.05 - 0.1 mm; the outer layer is a polypropylene protective shell with a shell wall thickness of 0.4 - 0.8 mm; the attractant is contained in the polyethylene slow-release bag; the attracting device releases the attractant to trap the pine sawyer into the sawyer trap and be captured. The hanging density of the sawyer trap is 3 - 5 per mu, the hanging height is 0.8 - 1.2 m, and the distance between the sawyer traps is 150 - 200 m.

[0058] The present invention will be further described in detail below in conjunction with specific embodiments. It should be emphasized that the following description is merely exemplary and not intended to limit the scope and application of the present invention.

[0059] The specific numerical values of other components in Examples 1 - 3 and Comparative Examples 1 - 3 except 95% ethanol are shown in Table 1, and the balance is made up to 100% with 95% ethanol.

[0060] Table 1 Specific numerical values of other components in Examples 1 - 3 and Comparative Examples 1 - 3 except 95% ethanol

[0061]

[0062] The preparation method of the pine sawyer attractant in Examples 1 - 3 and Comparative Examples 1 - 3 includes the following steps:

[0063] S1. Step-by-step mixing: Heat 95% ethanol to 40°C, and sequentially add (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, pinene, and camphene, with a stirring frequency of 200 rpm and stir for 20 min until evenly mixed;

[0064] S2. Ultrasonic dispersion: Add ocimene, longifolene, and (+)-limonene, and perform ultrasonic dispersion, setting the microwave power to 200 W and the time to 20 min;

[0065] S3. Static reaction: Add a synergist and a stabilizer to Examples 1 - 3, and perform a static reaction at 25°C for 2.5 h; no synergist and stabilizer are added to Comparative Examples 1 - 3;

[0066] S4. Slow-release encapsulation: Mix the mixed solution with porous silica microspheres at a mass ratio of 1:0.1, and prepare the attractant after adsorption.

[0067] The pine sawyer attractants prepared in Examples 1 - 3 and Comparative Examples 1 - 3 were subjected to a field application test. Each test treatment was set with 3 replicates, and the test site was the Guangxi State-owned Gaofeng Forest Farm.

[0068] The application method of the attractant in the product for trapping and killing pine sawyers in the field application test is as follows:

[0069] (1) The prepared pine sawyer attractant is encapsulated in the inner polyethylene slow-release bag of the slow-release device. The wall thickness of the slow-release bag is 0.08 mm, and the wall thickness of the outer polypropylene protective shell of the slow-release device is 0.6 mm to obtain the attracting device;

[0070] (2) Hang the attracting device at the middle position 20 cm from the top in the cross-shaped sawyer trap. The large board and the small board are crossed to form a cross shape, connected to the upper cover through the hook at the top, and connected to the funnel through the hook at the bottom. Put the bell mouth connecting the collecting bottle on the lower part of the funnel and connect the collecting bottle to prepare the product for trapping and killing pine sawyers. The hanging density of the sawyer trap is 3 per mu, the hanging height is 0.8 m, and the distance between the sawyer traps is 150 m. In this experiment, 3 mu of land was selected for each treatment for the experiment, that is, 3 replicates were set. The number of pine sawyers trapped in each treatment was the average of the 3 traps per mu of land. According to the weather conditions, a short-term experiment was carried out from May 1 to May 28, 2024, for a total of 28 days. The number of pine sawyers in the collecting bottle was observed every 7 days. After counting, the pine sawyers trapped by the 3 sawyer traps per mu of land were collected, and the results are shown in Table 2; A long-term experiment was carried out from May 1 to August 29, 2024, for a total of 120 days. The number of pine sawyers in the collecting bottle was observed every 30 days. After counting, the trapped pine sawyers were collected, and the results are shown in Table 3.

[0071] Table 2 Number of pine sawyers trapped by each trap in different treatments of the short-term experiment

[0072] Group Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 1 - 7 days 14 11 13 3 6 5 8 - 14 days 16 12 14 4 4 7 15 - 21 days 19 15 15 5 5 6 22 - 28 days 20 16 17 3 4 4 Total trapping amount 69 54 59 15 19 22

[0073] Table 3 Number of pine sawyers trapped by each trap in different treatments of the long-term experiment results

[0074] Group Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 1 - 30 days 68 59 64 13 16 17 31 - 60 days 73 62 67 9 12 14 61 - 90 days 46 39 40 5 7 5 90 - 120 days 14 8 12 0 0 0 Total trapping amount 201 168 183 27 35 36

[0075] From the result analysis of Table 2 and Table 3, in the results of the short-term test, the total trapping amounts of Examples 1-3 were 69, 54, and 59 respectively, while the total trapping amounts of Comparative Examples 1-3 were only 15, 19, and 22 respectively. The trapping effect of the examples was significantly better than that of the comparative examples, indicating that the complete formula of the present invention can attract Monochamus alternatus more efficiently in the short term. Comparative Example 1 had no synergist and stabilizer, and its trapping amount was the lowest, indicating that these two components have a positive effect on improving the attracting effect; Comparative Example 2 had no synergist, and Comparative Example 3 had no stabilizer. Their trapping amounts were between those of the examples and Comparative Example 1, further confirming the importance of the synergist and stabilizer respectively. In the results of the long-term test, the total trapping amounts of Examples 1-3 were 201, 168, and 183 respectively, and the total trapping amounts of Comparative Examples 1-3 were 27, 35, and 36 respectively. The examples still maintained a relatively high trapping amount in the long-term test, showing good long-term effectiveness. As time passed, the trapping amounts of Comparative Examples 1-3 decreased significantly. In particular, the trapping amounts of Comparative Examples 1 and 2 dropped to 0 from 90 to 120 days, while the examples still had a certain trapping amount, indicating that the stabilizer and synergist not only improve the short-term attracting effect but also ensure that the attractant continues to play a role during long-term use.

[0076] This is because the synergist can reduce the intermolecular force of the volatile substances, increase the diffusion rate of the volatile substances, expand the action radius of the attractant, and enable Monochamus alternatus at a farther distance to perceive the signal. The stabilizer can form a molecular protective film to inhibit the degradation of the volatile substances by environmental factors such as ultraviolet rays and ensure the effectiveness of the attractant. The two work together. The synergist enables more Monochamus alternatus to come into contact with the attractant, and the stabilizer ensures the stable existence of the attractant in the environment, jointly improving the attracting effect and the persistence period. The test results show that the attractant of the present invention has a significantly higher trapping amount than the comparative examples lacking the synergist and stabilizer in both the short-term and long-term tests. It has the characteristics of high-efficiency attraction, strong targeting, and long-term stability, can effectively solve the problems existing in the attractant for Monochamus alternatus in the prior art, provides an effective means for the prevention and control of pine wilt disease, and has good application prospects.

[0077] The above content is a further detailed description of the present invention in combination with specific implementation manners, and it cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention pertains, without departing from the concept of the present invention, several alternatives or modifications can be made to these described embodiments, and these alternative or modified forms should all be regarded as belonging to the protection scope of the present invention.

Claims

1. A pine sawyer attractant, characterized in that, It includes an attractant and a slow-release carrier, and the slow-release carrier is used to adsorb the attractant; wherein the attractant includes components with the following mass percentages: (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane: 5%-10%, pinene: 10%-20%, camphene: 5%-10%, ocimene: 10%-20%, longifolene: 10%-20%, (+)-limonene: 10%-20%, synergist: 1%-3%, stabilizer: 0.5%-2%, and the balance is made up to 100% with 95% ethanol.

2. The pine sawyer attractant according to claim 1, characterized in that, The pinene is a mixture of α-pinene and β-pinene, and the volume ratio of α-pinene to β-pinene is 1:2.8-3.

2.

3. The pine sawyer attractant according to claim 1, characterized in that, The synergist is γ-terpinene.

4. The pine sawyer attractant according to claim 1, characterized in that, The stabilizer is geranyl propionate.

5. The pine sawyer attractant according to claim 1, wherein, The slow-release carrier is porous silica microspheres, and the average pore diameter of the porous silica microspheres is 2-5 nm.

6. A preparation method of the pine sawyer attractant according to any one of claims 1-5, characterized in that, It includes the following steps: S1. Step-by-step mixing: Heat 95% ethanol to 35-40 °C, and sequentially add (1R)-2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, pinene, and camphene, and stir until evenly mixed; S2. Ultrasonic dispersion: Add ocimene, longifolene, and (+)-limonene, and perform ultrasonic dispersion for 15-25 min; S3. Static reaction: Add the synergist and the stabilizer, and perform a static reaction at 25 °C for 0.8-1.25 h; S4. Slow-release encapsulation: Mix the mixed solution with the porous silica microspheres according to a mass ratio of 1:0.1, and an attractant is obtained after adsorption.

7. The preparation method of a pine longicorn attractant according to claim 6, characterized in that, The stirring frequency in step S1 is 100-200 rpm, and the time is 15-25 min; the microwave power in step S2 is 150-250 W.

8. Use of the pine sawyer attractant according to any one of claims 1-5 or the pine sawyer attractant prepared by the preparation method according to any one of claims 6-7 in the preparation of a product for trapping and killing pine sawyers.

9. Use according to claim 8 in the preparation of a product for trapping and killing Monochamus alternatus Hope, characterized in that, The application method of the attractant in the preparation of a product for trapping and killing pine sawyers is as follows: (1) Encapsulate the prepared pine sawyer attractant in a slow-release device to obtain an attracting device; (2) Hang or place the attracting device in a longhorn beetle trap to prepare a product for trapping and killing pine sawyers.

10. Use according to claim 8 in the preparation of products for trapping and killing Monochamus alternatus, characterized in that, The slow-release device has a double-layer structure. The inner layer is a polyethylene slow-release bag with a wall thickness of 0.05-0.1 mm; the outer layer is a polypropylene protective shell with a shell wall thickness of 0.4-0.8 mm; the attractant is contained in the polyethylene slow-release bag; the attracting device releases the attractant to trap pine sawyers into the longhorn beetle trap to be captured. The hanging density of the longhorn beetle trap is 3-5 per mu, the hanging height is 0.8-1.2 m, and the distance between the longhorn beetle traps is 150-200 m.

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