Probiotic-fermented natural plant preparation for feeding as well as preparation method and application of probiotic-fermented natural plant preparation

Abstract

The invention relates to the technical field of poultry rearing preparations, and discloses a forage natural plant preparation through probiotic fermentation and a preparation method and application thereof, and the preparation method comprises the following steps: preparing a forage natural plant preparation micro powder mixture, preparing a forage natural plant preparation culture substrate, and preparing the forage natural plant preparation through probiotic fermentation. According to the invention, the forage natural plant preparation fermented by probiotics is used as a composite component, so that polysaccharide, starch, cellulose and active pharmaceutical ingredients in forage natural plants are promoted to be further converted and decomposed to form active ingredients which are easier to absorb, the number and activity of the probiotics are improved, the growth and release of the probiotics and metabolites thereof are facilitated, and the forage natural plant preparation can be used as a feed. The chicken bronchitis prevention and treatment effect is obviously higher than that of traditional Chinese veterinary medicine powder.

Description

Technical Field

[0001] The present invention relates to the technical field of poultry feeding preparations, in particular to a probiotic fermented feedable natural plant preparation and a preparation method and application thereof. Background Art

[0002] Infectious bronchitis (IB) is an acute, highly contagious viral respiratory disease in chickens caused by the infectious bronchitis virus (IBV). It causes decreased appetite, elevated body temperature, open-mouth breathing, coughing, and a runny nose. It often leads to decreased growth performance, increased mortality, and reduced egg production and eggshell quality in laying and breeding hens, resulting in significant economic losses. It is a major disease in the poultry industry.

[0003] Infectious bronchitis (IBV) primarily infects the respiratory tract, where the virus is excreted and transmitted through the air. It can also be transmitted through contaminated eggs, feed, water, and utensils. Infectious bronchitis occurs year-round, most often in winter and spring. IBV primarily infects chickens, and chickens of all ages are susceptible, with chicks being most susceptible. The incidence rate ranges from 70% to 100%, with a mortality rate of 10% to 40%. Infectious bronchitis spreads rapidly, and susceptible chickens exposed to the virus at almost the same time are susceptible to the disease. Crowded flocks, polluted air, damp ground, fluctuating temperatures, and a lack of vitamins and minerals in feed can contribute to the disease.

[0004] Avian infectious bronchitis has always been treated with antibiotics. On the one hand, the problem of antibiotic abuse leads to environmental pollution and affects human health. On the other hand, people pay more attention to the pursuit of green and safe living concepts. Therefore, there is an urgent need for a safe, green and harmless method to prevent and treat avian infectious bronchitis.

[0005] Therefore, there is an urgent need for a probiotic fermented edible natural plant preparation and its preparation method and application to solve the above technical problems. Summary of the Invention

[0006] The present invention aims to overcome the existing technical problems and provides a probiotic fermented edible natural plant preparation and a preparation method and application thereof.

[0007] To achieve the above object, the present invention is implemented according to the following technical solutions:

[0008] A method for preparing a probiotic fermented edible natural plant preparation comprises the following steps:

[0009] S1, preparation of a micronized mixture of natural plant preparations for feeding

[0010] The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 160-240 parts of plantain seeds, 160-240 parts of purslane, 160-240 parts of astragalus, 160-240 parts of honeysuckle, 160-240 parts of forsythia, 160-240 parts of dandelion, 160-240 parts of platycodon, and 80-160 parts of elsholtzia;

[0011] The above components are weighed according to the ratio, and then crushed in sequence and mixed to obtain a feedable natural plant preparation powder mixture;

[0012] S2, preparation of culture medium substrate for edible natural plant preparations

[0013] adding a culture substrate to the edible natural plant preparation micropowder mixture and mixing the mixture to obtain a culture substrate for the edible natural plant preparation;

[0014] S3, preparation of probiotic fermented natural plant preparations for feeding

[0015] The substrate of the edible natural plant preparation culture medium is sterilized by using high-pressure steam, inoculated with Bacillus subtilis after cooling, and cultured after the inoculation to obtain the probiotic fermented edible natural plant preparation.

[0016] Preferably, in step S1, each component is crushed to 100 mesh in sequence.

[0017] Preferably, in step S1, the feedable natural plant preparation powder mixture includes the following components in parts by weight: 220 parts of plantain seeds, 160 parts of purslane, 160 parts of astragalus, 220 parts of honeysuckle, 160 parts of forsythia, 200 parts of dandelion, 160 parts of platycodon, and 100 parts of elsholtzia.

[0018] Preferably, in step S2, the culture medium substrate includes glucose, peptone, yeast extract, and water; the amount of each component of the culture medium substrate added is based on the weight of the feedable natural plant preparation micropowder mixture used, and includes the following components: 5% glucose, 3% peptone, 2% yeast extract, and 85% water.

[0019] As used herein, the weight basis of the feedable natural botanical powder mixture refers to the percentage by mass of each component of the culture medium added. Specifically, the amounts of culture medium added are: glucose (5% by weight of the natural botanical powder mixture), peptone (3% by weight of the natural botanical powder mixture), yeast extract (2% by weight of the natural botanical powder mixture), and water (85% by weight of the natural botanical powder mixture).

[0020] Preferably, in step S3, the strain number of the Bacillus subtilis is CICC 20445.

[0021] Specifically, the strain of Bacillus subtilis was purchased from the China Industrial Culture Collection Center for Microorganisms, and the strain number is Bacillus subtilis CICC 20445.

[0022] Preferably, in step S3, the inoculation amount of Bacillus subtilis is: 5% of the weight of the natural plant preparation culture medium substrate for feed prepared in step S2 is inoculated with Bacillus subtilis liquid; the colony count of the Bacillus subtilis liquid is ≥1.0×10 11 cfu / mL.

[0023] Preferably, in step S3, after inoculation, the culture is carried out at a pH of 6 and a temperature of 37° C. for 12 hours.

[0024] Preferably, in step S3, the high-pressure steam sterilization condition is high-pressure sterilization at 121° C. for 20 minutes.

[0025] The present invention also includes a probiotic fermented edible natural plant preparation, which is prepared by the above preparation method.

[0026] The present invention also includes the use of a probiotic fermented edible natural plant preparation in preventing and treating chicken bronchitis.

[0027] Working principle:

[0028] Traditional Chinese medicine is used to prevent and treat various diseases due to its good efficacy, lack of side effects, absence of residue, and ability to enhance the body's immunity. Using traditional Chinese medicine antiviral drugs to reduce the harm of the disease, combined with enhanced ventilation and increased supply of multiple vitamins, can effectively prevent and treat infectious bronchitis in chickens. The present invention uses plantago, purslane, astragalus, honeysuckle, forsythia, tartar, platycodon, and elsholtzia; a culture substrate is prepared with the active ingredients from the plants, and Bacillus subtilis is inoculated into the edible natural plant culture substrate to produce a probiotic fermented edible natural plant preparation for preventing and treating bronchitis in chickens. Compared with the prior art, the present invention ferments edible natural plants with probiotics to form composite components, which promotes the further conversion and decomposition of polysaccharides, starch, cellulose, and active pharmaceutical ingredients in the edible natural plants to form more easily absorbed active ingredients. It also increases the number and activity of probiotics, which is beneficial to the growth and release of probiotics and their metabolites. The efficacy of the preparation in preventing and treating bronchitis in chickens is significantly higher than that of traditional Chinese veterinary medicine powders.

[0029] Beneficial effects:

[0030] The present invention uses probiotic fermentation of edible natural plant preparations as a composite component, which promotes the further conversion and decomposition of polysaccharides, starch, cellulose and active pharmaceutical ingredients in the edible natural plants to form more easily absorbed active ingredients, and also increases the number and activity of probiotics, which is beneficial to the growth and release of probiotics and their metabolites. The efficacy of the present invention in preventing and treating chicken bronchitis is significantly higher than that of traditional Chinese veterinary medicine powders. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a microscopic observation image of the bacteria obtained from recipe 1 in Experiment 3 of the present invention;

[0032] Figure 2 This is a microscopic observation of the bacteria obtained from recipe 2 in Experiment 3 of the present invention;

[0033] Figure 3 This is a microscopic observation image of the bacteria obtained from recipe 3 in experiment 3 of the present invention;

[0034] Figure 4 This is a microscopic observation of the bacteria obtained from recipe 4 in Experiment 3 of the present invention;

[0035] Figure 5 This is a microscopic observation picture of the bacteria obtained from recipe 5 in experiment 3 of the present invention;

[0036] Figure 6 This is a microscopic observation image of the bacteria obtained under condition 1 in experiment 4 of the present invention;

[0037] Figure 7 This is a microscopic observation image of the bacteria obtained under condition 2 in experiment 4 of the present invention;

[0038] Figure 8 This is a microscopic observation image of the bacteria obtained under condition 3 in experiment 4 of the present invention;

[0039] Figure 9 This is a microscopic observation image of the bacteria obtained under condition 4 in experiment 4 of the present invention;

[0040] Figure 10 This is a microscopic observation image of the bacteria obtained under condition 5 in experiment 4 of the present invention;

[0041] Figure 11 This is a microscopic observation image of the bacteria obtained under condition 6 in experiment 4 of the present invention;

[0042] Figure 12 This is a microscopic observation image of the bacteria obtained under condition 7 in experiment 4 of the present invention;

[0043] Figure 13 This is a microscopic observation image of the bacteria obtained under condition eight in experiment 4 of the present invention;

[0044] Figure 14This is a microscopic observation image of the bacteria obtained under condition nine in experiment 4 of the present invention. DETAILED DESCRIPTION

[0045] The present invention will be further described below with reference to specific embodiments. The illustrative embodiments and descriptions of the present invention are intended to explain the present invention but are not intended to limit the present invention.

[0046] All raw materials of the present invention are not particularly limited in their sources and can be purchased from the market or prepared according to conventional methods well known to those skilled in the art.

[0047] There is no particular limitation on the purity of all raw materials in the present invention, and the present invention preferably adopts conventional purity used in the art.

[0048] The devices used in the present invention are not particularly limited and can be devices commonly used in the art.

[0049] Example 1

[0050] A method for preparing a probiotic fermented edible natural plant preparation comprises the following steps:

[0051] S1, preparation of a micronized mixture of natural plant preparations for feeding

[0052] The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 220 parts of plantain seeds, 160 parts of purslane, 160 parts of astragalus, 220 parts of honeysuckle, 160 parts of forsythia, 200 parts of dandelion, 160 parts of platycodon and 100 parts of elsholtzia.

[0053] The above components are weighed according to the ratio, and then crushed into 100 mesh in sequence, and mixed to obtain a feedable natural plant preparation powder mixture;

[0054] S2, preparation of culture medium substrate for edible natural plant preparations

[0055] A culture medium substrate is added to a mixture of micropowders of a edible natural plant preparation and the mixture is mixed to obtain a culture medium substrate for the edible natural plant preparation; the culture medium substrate comprises glucose, peptone, yeast extract, and water; the amount of the culture medium substrate added is based on the weight of the used micropowder mixture of the edible natural plant preparation, and comprises the following components: 5% glucose, 3% peptone, 2% yeast extract, and 85% water.

[0056] S3, preparation of probiotic fermented natural plant preparations for feeding

[0057] The substrate of the culture medium of the natural plant preparation for feed is sterilized by high-pressure steam at 121° C. for 20 minutes, and after cooling, Bacillus subtilis is inoculated therein. After inoculation, the substrate is cultured at a pH of 6 and a temperature of 37° C. for 12 hours to obtain the probiotic fermented natural plant preparation for feed; the inoculation amount of Bacillus subtilis is 5% of the weight of the substrate of the culture medium of the natural plant preparation for feed, and the number of inoculated colonies is 1.0×10 11 cfu / mL of Bacillus subtilis liquid.

[0058] The strain of Bacillus subtilis used in this example was purchased from the China Industrial Microbiological Culture Collection Center, and the strain number is Bacillus subtilis CICC 20445.

[0059] Example 2

[0060] The difference between this embodiment and embodiment 1 is that the edible natural plant preparation includes the following components in parts by weight: 180 parts of plantain seeds, 240 parts of purslane, 200 parts of astragalus, 180 parts of honeysuckle, 240 parts of forsythia, 160 parts of dandelion, 160 parts of platycodon, and 100 parts of elsholtzia; the rest is the same as in embodiment 1.

[0061] Example 3

[0062] The difference between this embodiment and embodiment 1 is that the edible natural plant preparation includes the following components in parts by weight: 240 parts of plantain seeds, 160 parts of purslane, 240 parts of astragalus, 160 parts of honeysuckle, 180 parts of forsythia, 240 parts of dandelion, 160 parts of platycodon, and 120 parts of elsholtzia; the rest is the same as in embodiment 1.

[0063] Comparative Example 1

[0064] The difference between this comparative example and Example 1 is that the feedable natural plant preparation includes the following components in parts by weight: 160 parts of Plantago seeds, 160 parts of Portulaca oleracea, 200 parts of Astragalus, 200 parts of Honeysuckle, 120 parts of Forsythia, 200 parts of Taraxacum, 100 parts of Platycodon grandiflorum, and 80 parts of Elsholtzia ciliata; the rest is the same as in Example 1.

[0065] Comparative Example 2

[0066] The difference between this comparative example and Example 1 is that the feedable natural plant preparation includes the following components in parts by weight: 160 parts of Plantago seeds, 180 parts of Portulaca oleracea, 200 parts of Astragalus, 180 parts of Honeysuckle, 160 parts of Forsythia, 180 parts of Taraxacum, 120 parts of Platycodon grandiflorum, and 80 parts of Elsholtzia ciliata; the rest is the same as in Example 1.

[0067] Comparative Example 3

[0068] The difference between this comparative example and Example 1 is that the feedable natural plant preparation includes the following components in parts by weight: 200 parts of Plantago seeds, 160 parts of Portulaca oleracea, 180 parts of Astragalus, 200 parts of Honeysuckle, 120 parts of Forsythia, 160 parts of Taraxacum, 120 parts of Platycodon grandiflorum, and 100 parts of Elsholtzia ciliata; the rest is the same as in Example 1.

[0069] Comparative Example 4

[0070] This comparative example directly used the feedable natural plant preparation powder mixture described in Example 1;

[0071] The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 220 parts of plantain seeds, 160 parts of purslane, 160 parts of astragalus, 220 parts of honeysuckle, 160 parts of forsythia, 200 parts of dandelion, 160 parts of platycodon and 100 parts of elsholtzia.

[0072] Comparative Example 5

[0073] This comparative example directly used the feedable natural plant preparation powder mixture described in Example 2;

[0074] The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 180 parts of plantain seeds, 240 parts of purslane, 200 parts of astragalus, 180 parts of honeysuckle, 240 parts of forsythia, 160 parts of dandelion, 160 parts of platycodon and 100 parts of elsholtzia.

[0075] Comparative Example 6

[0076] This comparative example directly used the edible natural plant preparation powder mixture described in Example 3;

[0077] The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 240 parts of plantain seeds, 160 parts of purslane, 240 parts of astragalus, 160 parts of honeysuckle, 180 parts of forsythia, 240 parts of dandelion, 160 parts of platycodon and 120 parts of elsholtzia.

[0078] Experiment 1

[0079] 180 chickens diagnosed with infectious bronchitis were taken, 20 in each group, and divided into 9 groups. Based on the weight of the fed diet, 2% of the feedable natural plant preparations prepared in Examples 1 to 3, the feedable natural plant preparations prepared in Comparative Examples 1 to 3, and the feedable natural plant preparation powder mixture of Comparative Examples 4 to 6 were added respectively. After feeding for 5 days (120 hours) based on this standard, the chickens were continuously observed until the bronchitis turned negative (the detected negative turning time included the 5 days of feeding), and the time of negative turning was recorded, wherein the course of the disease was observed by anatomical experiments and test kits.

[0080] The data obtained is as follows:

[0081] Tracheal gurgling Purulent secretions from the trachea Runny nose Test results Comparative Example 1 have have have Negative after 35 days Comparative Example 2 have none have Negative after 33 days Comparative Example 3 have have none Negative after 35 days Comparative Example 4 have none have Negative after 35 days Comparative Example 5 have have none Negative after 39 days Comparative Example 6 have have have Negative after 37 days Example 1 none none none Negative after 32 days Example 2 none have none Negative after 34 days Example 3 none have have Negative after 33 days

[0082] From the above experimental data, it can be seen that the plant preparation prepared in Example 1 is more conducive to the treatment and recovery of infectious bronchitis in chickens. After consuming it for 5 days, the results turned negative after 32 days.

[0083] Experiment 2

[0084] Infectious bronchitis test: 2000 chickens were divided into 10 groups, one group of 200 chickens was a blank control group, and the remaining nine groups of 200 chickens were added to the diet by weight. The plant preparations prepared in Examples 1 to 3 and Comparative Examples 1 to 6 were added. After 10 days of feeding, the number of chickens with the disease was counted.

[0085] Number of patients with initial symptoms (individuals) Number of patients with symptoms at the end of the period (only) Difference (only) Comparative Example 1 25 9 16 Comparative Example 2 19 8 11 Comparative Example 3 22 12 10 Comparative Example 4 26 16 10 Comparative Example 5 27 20 7 Comparative Example 6 24 18 6 Example 1 25 5 20 Example 2 28 19 9 Example 3 33 21 11 Blank control group 30 93 -63

[0086] From the above experimental data, it can be seen that the plant preparation prepared in Example 1 has the strongest inhibitory effect on the spread of chicken infectious bronchitis.

[0087] Experiment 3

[0088] Culture medium substrate test

[0089] Experimental conditions: The following culture medium substrates were added to an equal weight of the mixture of micropowders of the natural plant preparation for feed obtained in step S1 of Example 1 to obtain a culture medium substrate for the natural plant preparation for feed. The culture was carried out under the conditions of pH 5.5, culture temperature 37°C, inoculation size 4%, and culture time 12 hours. After the culture was completed, a viable bacteria test was performed.

[0090] The same Bacillus subtilis liquid was used for inoculation, and the colony count of the Bacillus subtilis liquid used was ≥1.0×10 11 cfu / mL; the percentage of inoculation amount is weight percentage, and the basis of the weight percentage is the weight of the natural plant preparation culture medium substrate used.

[0091] The percentage of each component in each formula is weight percentage, and the basis of the weight percentage is the weight of the feedable natural plant preparation powder mixture used.

[0092] Formula 1: glucose 5%, peptone 3%, yeast extract 2%, water 85%;

[0093] Formula 2: 4% glucose, 3% peptone, 3% yeast extract, 85% water;

[0094] Formula 3: glucose 5%, peptone 3%, yeast extract 2%, water 90%;

[0095] Formula 4: 3% glucose, 4% peptone, 4% yeast extract, 85% water;

[0096] Recipe 5: Glucose 5%, peptone 4%, yeast extract 3%, water 80%.

[0097] The test results are shown in the following table:

[0098] formula Viable bacteria count Recipe 1 <![CDATA[4.5*10 11 ]]> Recipe 2 <![CDATA[3.6*10 11 ]]> Recipe 3 <![CDATA[3.9*10 11 ]]> Recipe 4 <![CDATA[3.2*10 11 ]]> Recipe 5 <![CDATA[2.3*10 11 ]]>

[0099] like Figures 1 to 5 Shown are microscopic observation images of the bacteria obtained from recipes 1 to 5 in Experiment 3 of the present invention.

[0100] Conclusion: Formula 1 is the most effective for Bacillus subtilis type I fermentation.

[0101] Experiment 4

[0102] Fermentation condition test

[0103] The following culture medium substrate (5% glucose, 3% peptone, 2% yeast extract, 85% water) was added to an equal weight of the natural plant preparation powder mixture obtained in step S1 of Example 1 to obtain a natural plant preparation culture medium substrate for feed. The culture was carried out under the following conditions, and a viability test was performed after the culture was completed.

[0104] The number of colonies used for inoculation was 1.0×10 11 cfu / mL of Bacillus subtilis bacterial liquid; the percentage of inoculation amount is weight percentage, and the basis of the weight percentage is the weight of the natural plant preparation culture medium substrate used.

[0105] The percentage of each component in each formula is weight percentage, and the basis for the weight percentage is the weight of the feedable natural plant preparation powder mixture used. Culture medium formula: glucose 5%, peptone 3%, yeast extract 2%, water 85%.

[0106] Condition 1: pH 5.5, culture temperature 36°C, inoculation size 3%, culture time 11 hours;

[0107] Condition 2: pH 5.5, culture temperature 36°C, inoculation size 4%, culture time 11 hours;

[0108] Condition 3: pH 5.5, culture temperature 36°C, inoculation size 5%, and culture time 11 hours;

[0109] Condition 4: pH 6.0, culture temperature 37°C, inoculation size 3%, culture time 12 hours;

[0110] Condition 5: pH 6.0, culture temperature 37°C, inoculation size 4%, culture time 12 hours;

[0111] Condition 6: pH 6.0, culture temperature 37°C, inoculation size 5%, culture time 12 hours;

[0112] Condition 7: pH 6.0, culture temperature 38°C, inoculation size 3%, culture time 13 hours;

[0113] Condition 8: pH 6.0, culture temperature 38°C, inoculation size 4%, culture time 13 hours;

[0114] Condition nine: pH 6.0, culture temperature 38°C, inoculation size 5%, and culture time 13 hours.

[0115] The test results are shown in the following table:

[0116]

[0117]

[0118] like Figures 6 to 14 Shown are microscopic observation images of the bacteria obtained under conditions one to nine in Experiment 4 of the present invention.

[0119] Conclusion: Culture condition six has the best adaptability to the strain and the bacterial growth and reproduction quantity is the highest.

[0120] The technical solution of the present invention is not limited to the above-mentioned specific embodiments. Any technical variations made according to the technical solution of the present invention fall within the protection scope of the present invention.

Claims

1. A method for preparing a natural plant preparation fermented with probiotics for feeding, characterized by: Including the following step: S1, preparation of a micronized mixture of natural plant preparations for feeding The feedable natural plant preparation powder mixture comprises the following components in parts by weight: 160-240 parts of plantain seeds, 160-240 parts of purslane, 160-240 parts of astragalus, 160-240 parts of honeysuckle, 160-240 parts of forsythia, 160-240 parts of dandelion, 160-240 parts of platycodon, and 80-160 parts of elsholtzia; The above components are weighed according to the ratio, and then crushed in sequence and mixed to obtain a feedable natural plant preparation powder mixture; S2, preparation of culture medium substrate for edible natural plant preparations adding a culture substrate to the edible natural plant preparation micropowder mixture and mixing the mixture to obtain a culture substrate for the edible natural plant preparation; S3, preparation of probiotic fermented natural plant preparations for feeding The substrate of the edible natural plant preparation culture medium is sterilized by using high-pressure steam, inoculated with Bacillus subtilis after cooling, and cultured after the inoculation to obtain the probiotic fermented edible natural plant preparation.

2. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S1, each component is crushed to 100 mesh in sequence.

3. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S1, the feedable natural plant preparation powder mixture includes the following components in parts by weight: 220 parts of plantain seeds, 160 parts of purslane, 160 parts of astragalus, 220 parts of honeysuckle, 160 parts of forsythia, 200 parts of dandelion, 160 parts of platycodon, and 100 parts of elsholtzia.

4. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S2, the culture medium substrate includes glucose, peptone, yeast extract, and water; the amount of each component of the culture medium substrate added is based on the weight of the feedable natural plant preparation powder mixture used, and includes the following components: 5% glucose, 3% peptone, 2% yeast extract, and 85% water.

5. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S3, the strain number of the Bacillus subtilis is CICC 20445.

6. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S3, the inoculation amount of Bacillus subtilis is: 5% of the weight of the natural plant preparation culture medium substrate for feed prepared in step S2 is inoculated with Bacillus subtilis liquid; the colony count of the Bacillus subtilis liquid is ≥1.0×10 11 cfu / mL.

7. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In the step S3, after inoculation, the culture is carried out at a pH of 6 and a temperature of 37° C. for 12 hours.

8. The method for preparing a probiotic fermented edible natural plant preparation according to claim 1, characterized in that: In step S3, the high-pressure steam sterilization condition is 121° C. high-pressure sterilization for 20 minutes.

9. A probiotic fermented natural plant preparation for feeding, characterized by: The invention is prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the edible natural plant preparation fermented by probiotics according to claim 9 in preventing and treating bronchitis in chickens.

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