Incubator for fermenting monascus purpureus by using monascus purpureus strain, fermentation process and application

Abstract

The invention relates to the technical field of strain fermentation, in particular to an incubator for monascus purpureus strain fermentation of monascus purpureus monascus as well as a fermentation process and application of the monascus purpureus strain fermentation of monascus purpureus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus monascus. A plurality of transverse ropes and longitudinal ropes are distributed on the inner side of the oscillation table in the horizontal direction, when culture bottles are placed in gaps between the transverse ropes and the longitudinal ropes, if the culture bottles do not need to be sealed strictly, the transverse ropes and the longitudinal ropes elastically abut against the outer sides of the culture bottles to be used for limiting and fixing the culture bottles, and if the requirement for sealing in the fermentation culture process is high, the transverse ropes and the longitudinal ropes can be tightly pressed against the outer sides of the culture bottles. The bottom end of the sealing cover is buckled on the opening in the top end of the culture bottle in a covering mode by pulling the sealing cover downwards, at least one of the transverse rope and the longitudinal rope is arranged in the strip-shaped groove in a penetrating mode by pulling the sealing cover manually, the sealing cover is tightly buckled on the culture bottle in a covering mode, and efficient ventilation is achieved through the ventilation pipe.

Description

Technical Field

[0001] The invention relates to the technical field of strain fermentation, in particular to a culture vessel for fermenting red yeast rice with a purple Monascus strain, a fermentation process and an application thereof. Background Art

[0002] Red yeast rice is a kind of rice produced by inoculating purple Monascus purpureus fungus of the Aspergillus family on the seed kernel (rice) of the grass plant Oryza sativa, and then artificially cultivating and fermenting it. The rice grains are purple or red, and it has extremely high commercial value and broad development prospects. The preparation process of red yeast rice involves the cultivation and fermentation of the strain, so the fermentation culture vessel is one of the key equipment in the preparation. In the prior art, such as the automatic cultivation device for optimizing and enriching the phenol-reducing strain GY8 disclosed in the Chinese patent document CN206666500U, and the existing conventional horizontal culture vessels, vertical culture vessels, etc., all adopt an oscillator provided in a closed box, and the culture bottle with the prepared strain culture solution is placed on the oscillator, and the temperature and humidity conditions in the closed box are controlled to carry out fermentation and cultivation. When necessary, the oscillator is axially rotated to drive the culture bottle to oscillate. During the fermentation and cultivation process, it may be necessary to The opening of the culture bottle is sealed, and the inside of the culture bottle may also need to be ventilated. In the existing technology, a sterilization sealing film is usually used to cover the opening of the sealed culture bottle, and the sterilization sealing film is fastened to the neck of the culture bottle with a rubber band or the like. When ventilation is required, the sterilization sealing film is manually removed, and the ventilation is completed by active or passive circulation ventilation in the closed box. However, in the actual research and development and production process, if the sealing requirements during the fermentation culture process are high, the sealing effect of the sterilization sealing film is average, the disassembly and assembly process is inconvenient, and the active or passive circulation ventilation method in the closed box is also average, and the efficiency is not high. Summary of the Invention

[0003] In view of this, the purpose of the present invention is to propose a culture vessel, fermentation process and application for fermenting red yeast rice with a purple Monascus strain, so as to solve the problems of poor sealing effect and inconvenient operation of the culture bottle in the existing process of fermenting red yeast rice with a purple Monascus strain, as well as poor ventilation effect and low efficiency of the culture bottle.

[0004] Based on the above objectives, the present invention provides a culture vessel for fermenting red yeast rice using a purple Monascus strain, comprising a vertical culture vessel body:

[0005] The vertical incubator is provided with an incubator, and an oscillating table is provided at the bottom end of the incubator. The oscillating table is designed as a groove structure with an open top for placing culture bottles, and the culture bottles are designed with an open top.

[0006] The top of the inner part of the incubator is movably connected to a plurality of covers, the top of the covers is connected to a ventilation pipe, the bottom of the covers is flared, and the top of the covers is provided with a strip groove;

[0007] A plurality of transverse ropes are arranged in parallel along the horizontal direction on the inner side of the oscillation table, and the two ends of the transverse ropes are elastically connected to the inner side of the oscillation table. A plurality of longitudinal ropes are arranged in parallel along the horizontal direction on the inner side of the oscillation table, and the two ends of the longitudinal ropes are elastically connected to the inner side of the oscillation table. The length direction of the longitudinal ropes is perpendicular to the length direction of the transverse ropes. When a culture bottle is placed in the gap between the transverse ropes and the longitudinal ropes, the gap between the transverse ropes and the longitudinal ropes is used to elastically press against the outer side of the culture bottle. When the cover is pulled downward so that the bottom end of the cover is buckled on the top opening of the culture bottle, at least one of the transverse ropes and the longitudinal ropes is passed through the strip groove to elastically press against the top of the culture bottle downward.

[0008] Preferably, the ventilation pipe includes an air inlet pipe located on one side of the cover, and an air outlet pipe located on the other side of the cover, wherein the bottom end of the air inlet pipe and the bottom end of the air outlet pipe are arranged in a height offset manner.

[0009] Preferably, the ventilation pipe vertically passes through the cover, and the portion passing through the top of the cover upwards is designed as a hose structure, and the portion passing through the top of the cover downwards is designed as a hard tube structure.

[0010] Preferably, the longitudinal rope is located below the transverse rope, and a limiting block is slidably connected to the longitudinal rope. Limit pins are vertically provided on the four corners of the edge of the limit block. A first monitoring component and a second monitoring component are respectively provided on the front and back sides of the limit block. When the culture bottle is placed on the oscillation table along the gap between the transverse rope and the longitudinal rope, the limiting block is slid so that the limiting pin is pressed against the outside of the culture bottle, and the first monitoring component and the second monitoring component face the two sides of the culture bottle respectively to monitor the fermentation culture status in the bottle.

[0011] Preferably, a strip flange is provided in parallel in the middle of one side of the limiting block, and a threading hole for the longitudinal rope to pass through is provided in parallel in the middle of the strip flange.

[0012] Preferably, pin barrels are provided on the four corners of the edge of the limit block. The pin barrels are arranged perpendicular to the limit block and are designed with open ends. The limit pins are elastically inserted into the pin barrels, and the ends of the limit pins pass through the openings of the pin barrels.

[0013] Preferably, the first monitoring component and the second monitoring component are one or more of a temperature sensor, a camera, and a humidity sensor.

[0014] The present invention also provides a fermentation process for fermenting red yeast rice using a purple Monascus strain, comprising the following steps:

[0015] Prepare a strain activation solution, inoculate the Monascus purpurogenum strain on a strain culture medium for culture activation, wash off the bacterial spores on the strain culture medium with sterile physiological saline, break up the spore aggregates and make them uniform, and obtain a spore suspension;

[0016] The spore suspension was inoculated into a culture bottle containing a liquid seed culture medium, and the culture bottle was placed on a shaking table. The culture was shaken and cultured at 30°C and 180 rpm for 48 hours to obtain an activated solution of the Monascus purpurogenum strain.

[0017] Under aseptic conditions, a culture bottle containing a culture matrix is filled with an activation liquid of the red aspergillus strain for fermentation and culture. The cap is pulled out downward and buckled onto the top opening of the culture bottle, the transverse rope is pulled upward and inserted into the strip groove to elastically press downward against the top of the culture bottle, and the limit block on the longitudinal rope is slid so that the limit pins on the four corners of the limit block edge are elastically pressed against the outside of the culture bottle. The first monitoring member and the second monitoring member on the front and back sides of the limit block are respectively facing the two sides of the culture bottle for real-time monitoring of the fermentation and culture status in the bottle. The fermentation and culture adopts variable temperature culture, and the variable temperature culture conditions are as follows: after culturing at 30-32°C for 3-5 days, culturing at 25-27°C for 15-17 days, and the humidity is 60%-75%. The culture bottle is ventilated through an air exchange tube for 30 minutes per day. After the variable temperature culture is completed, the culture matrix is dried at 40-45°C to obtain a final red yeast rice product.

[0018] The present invention also provides a use of a red yeast rice product prepared by a fermentation process of red yeast rice with a purple Monascus strain in the preparation of lipid-lowering drugs and health functional foods.

[0019] The beneficial effects of the present invention are as follows: a plurality of covers are movably connected to the inner top end of the incubator, the top end of the covers is connected to a ventilation tube, a strip groove is opened at the top end of the covers, a plurality of horizontal ropes are arranged in parallel along the horizontal direction on the inner side of the oscillation table, a plurality of longitudinal ropes are arranged in parallel along the horizontal direction on the inner side of the oscillation table, the length direction of the longitudinal ropes is perpendicular to the length direction of the horizontal ropes, when a culture bottle is placed in the middle of the gap between the horizontal ropes and the longitudinal ropes, if the culture bottle does not need to be strictly sealed, the horizontal ropes and the longitudinal ropes are elastically pressed against the outer side of the culture bottle to limit and fix the culture bottle; if the sealing requirement is higher during the fermentation culture process, the cover is pulled downward so that the bottom end of the cover is buckled on the top opening of the culture bottle, and then manually pulled to make at least one of the horizontal ropes and the longitudinal ropes pass through the strip groove, so that the cover is tightly buckled on the culture bottle and efficient ventilation is achieved through the ventilation tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 Schematic diagram of the overall structure of the vertical incubator body of the present invention;

[0022] Figure 2 This is a schematic diagram of the front view of the structure of the inner side transverse rope of the oscillation table of the present invention;

[0023] Figure 3 For the present invention Figure 2 A magnified schematic diagram of point A in the middle;

[0024] Figure 4 For the present invention Figure 2 A magnified schematic diagram of point B in the middle;

[0025] Figure 5 Schematic diagram of the top view of the cover of the present invention;

[0026] Figure 6 Schematic diagram of the front view of the longitudinal rope inside the oscillation table of the present invention;

[0027] Figure 7 For the present invention Figure 6 The enlarged schematic diagram of point C in the middle;

[0028] Figure 8 For the present invention Figure 6 The enlarged schematic diagram of point D in the middle;

[0029] Figure 9 Schematic diagram of the top view of the oscillation table without the limiting block of the present invention;

[0030] Figure 10 Schematic diagram of the top view of the oscillating table with limiting blocks of the present invention;

[0031] Figure 11 This is a schematic diagram of the top view of the oscillating table when a culture bottle is placed in the oscillating table of the present invention;

[0032] Figure 12 It is a schematic top view of the structure when the cover of the present invention is buckled on the culture bottle and the limiting pin is pressed against the outer side of the culture bottle.

[0033] The following are marked in the figure:

[0034] 1. Vertical incubator body; 100. Glass window; 2. Incubator; 3. Oscillating table; 4. Culture bottle; 5. Cover; 50. Sealing gasket; 51. Strip groove; 52. Pull rope; 6. Ventilation tube; 61. Inlet pipe; 62. Outlet pipe; 7. Horizontal rope; 8. Vertical rope; 9. Limit block; 90. Threading hole; 91. Strip flange; 10. Limit pin; 11. First monitoring component; 12. Second monitoring component; 13. Pin barrel. DETAILED DESCRIPTION

[0035] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to specific embodiments.

[0036] It should be noted that, unless otherwise defined, the technical or scientific terms used in the present invention should have the usual meanings understood by people with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0037] like Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 As shown, a culture vessel for fermenting red yeast rice with a purple Monascus strain comprises a vertical culture vessel body 1, an incubator 2 is provided in the vertical culture vessel body 1, an oscillating table 3 is provided at the inner bottom end of the incubator 2, the oscillating table 3 is designed as a groove structure with an open top, for placing a culture bottle 4, the culture bottle 4 is designed as a top opening, a plurality of covers 5 are movably connected to the inner top end of the incubator 2, a ventilation tube 6 is connected to the top end of the cover 5, the bottom end of the cover 5 is designed as a flared mouth, a strip groove 51 is provided at the top end of the cover 5, a plurality of horizontal ropes 7 are arranged in parallel along the horizontal direction on the inner side of the oscillating table 3, and the ends of the horizontal ropes 7 are connected to the inner top end of the incubator 2. It is elastically connected to the inner side of the oscillation table 3. A plurality of longitudinal ropes 8 are arranged in parallel along the horizontal direction on the inner side of the oscillation table 3. Both ends of the longitudinal ropes 8 are elastically connected to the inner side of the oscillation table 3. The length direction of the longitudinal ropes 8 is perpendicular to the length direction of the transverse ropes 7. When the culture bottle 4 is placed in the gap between the transverse ropes 7 and the longitudinal ropes 8, the ropes are used to elastically press against the outer side of the culture bottle 4. When the cover 5 is pulled downward so that the bottom end of the cover 5 is buckled on the top opening of the culture bottle 4, at least one of the transverse ropes 7 and the longitudinal ropes 8 passes through the strip groove 51 and is used to elastically press against the top of the culture bottle 4 downward.

[0038] The present invention is based on the existing conventional vertical incubator body 1, which is provided with an incubator 2. The inner bottom end of the incubator 2 is provided with an oscillating table 3. The oscillating table 3 is designed as a groove structure with an opening at the top for placing a culture bottle 4. The culture bottle 4 is designed with an opening at the top. The vertical incubator body 1 is located at the front end of the incubator 2 and is provided with an opening door. A transparent glass window 100 is provided in the middle of the opening door. When in use, the door is opened and the culture bottle 4 is placed on the oscillating table 3 at the bottom end of the incubator 2. The door is closed and certain temperature and humidity conditions and timing time are set to carry out fermentation culture. If necessary, the oscillation speed of the oscillating table 3 is set so that the oscillating table 3 rotates axially and oscillates. Specifically, A temperature and humidity control system is also provided in the incubator 2 of the existing conventional vertical incubator, including conventional heating elements such as resistance wire / PTC heaters evenly distributed on the side walls or bottom of the box body, conventional cooling elements such as circulating refrigerants evenly distributed on the side walls or bottom of the box body, conventional humidifiers and dehumidifiers, and air vents for passive ventilation, or circulating fans for active ventilation, ultraviolet germicidal lamps for disinfection and sterilization, temperature and humidity sensors, cameras and other components for monitoring, as well as conventional driving components such as a driving motor. The driving motor is provided at the bottom end of the vertical incubator body 1 and is used to drive the oscillation table 3 to axially rotate and oscillate.

[0039] In particular, a plurality of covers 5 are movably connected to the inner top of the incubator 2. For the simultaneous use of multiple culture bottles 4, the top of the cover 5 is connected to a ventilation tube 6, wherein the top of the cover 5 can be connected to a drawstring 52, and a component such as a drum for winding the drawstring 52 can be provided at the top of the vertical incubator body 1. By driving the drum to rotate and rewind, the corresponding drawstring 52 is driven to rewind, so that the corresponding cover 5 is retracted on the inner top of the incubator 2. The culture bottles 4 are designed in a conventional conical bottle structure. The top opening of the culture bottles 4 is a circular opening shape, and the cover 5 is designed in a conical cylinder structure with a corresponding bottom opening, and the bottom end of the cover 5 is a flared design, which is suitable for culture bottles 4 with different opening sizes. More preferably, the inner wall of the cover 5 is A sealing gasket 50 is affixed to the top for sealing against the top opening of the culture bottle 4. A strip groove 51 is provided on the top of the sealing cap 5. A plurality of transverse ropes 7 are arranged in parallel along the horizontal direction on the inner side of the oscillation table 3. The two ends of the transverse rope 7 are elastically connected to the inner side of the oscillation table 3. A plurality of longitudinal ropes 8 are arranged in parallel along the horizontal direction on the inner side of the oscillation table 3. The two ends of the longitudinal rope 8 are elastically connected to the inner side of the oscillation table 3. Specifically, the two ends of the transverse rope 7 and the longitudinal rope 8 can be inserted into the inner side of the oscillation table 3 and connected with existing conventional components such as springs, elastic ropes, etc., or the two ends of the transverse rope 7 and the longitudinal rope 8 are directly made of springs, elastic ropes, spring wires and other components. The length direction of the longitudinal rope 8 is perpendicular to the length direction of the transverse rope 7, so that when viewed from above, Figure 9 、 Figure 10As shown, the transverse ropes 7 and the longitudinal ropes 8 form a crisscross mesh shape. When the culture bottle 4 is placed in the gap between the transverse ropes 7 and the longitudinal ropes 8, the gap between the transverse ropes 7 and the longitudinal ropes 8 is smaller than the outer diameter of the culture bottle 4, so that the culture bottle 4 does not need to be sealed, or the culture bottle 4 is only sealed by a sterilized sealing film. Figure 11 As shown, the transverse rope 7 and the longitudinal rope 8 are elastically pressed against the outer side of the culture bottle 4 to limit and fix the culture bottle 4. If the sealing requirement during the fermentation culture process is high, the cover 5 is manually pulled downward and the pull rope 52 is pulled to drive the rotating drum to rotate and unwind, so that the bottom end of the cover 5 is buckled on the top opening of the culture bottle 4. At this time, it is manually pulled again to make at least one of the transverse rope 7 and the longitudinal rope 8 pass through the strip groove 51, as shown in FIG. Figure 1 、 Figure 2 、 Figure 6 、 Figure 12 As shown, the downward elastic force of the transverse rope 7 and the longitudinal rope 8 allows the cover 5 to be tightly fastened on the culture bottle 4, and the sealing gasket 50 to be tightly sealed against the top opening of the culture bottle 4, thereby achieving a better sealing effect. On the other hand, the downward elastic force against the top of the culture bottle 4 also facilitates the stable positioning and fixation of the culture bottle 4, making operation convenient.

[0040] For example Figure 12 As shown, the transverse rope 7 is pulled upward and inserted into the strip groove 51, and is used to elastically press downward against the top of the culture bottle 4, and the longitudinal rope 8 is elastically pressed against the outside of the culture bottle 4, or, for example, the longitudinal rope 8 is pulled upward and inserted into the strip groove 51, and is used to elastically press downward against the top of the culture bottle 4, and the transverse rope 7 is elastically pressed against the outside of the culture bottle 4, or both the transverse rope 7 and the longitudinal rope 8 are pulled upward and inserted into the strip groove 51, and are used to elastically press downward against the top of the culture bottle 4;

[0041] Among them, optional, such as Figure 5 As shown, the strip grooves 51 can be symmetrically arranged on both sides of the top of the cover 5, or symmetrically arranged on the four sides of the left, right, front and back of the top of the cover 5, for the two horizontal ropes 7 and the two longitudinal ropes 8 to pass through at the same time.

[0042] Among them, optionally, an air pump can be provided in the vertical culture vessel body 1 for connecting to the outer end of the ventilation tube 6. A switch valve can also be provided on the outer end of the ventilation tube 6. When sealing is required, the switch valve and the air pump are closed. When ventilation is required, the switch valve and the air pump are opened to directional ventilate the culture bottle 4, which has better ventilation effect and higher efficiency.

[0043] In an embodiment of the present invention, optionally, Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5As shown, the ventilation tube 6 includes an air inlet tube 61 located on one side of the cover 5, and an air outlet tube 62 located on the other side of the cover 5, wherein the bottom end of the air inlet tube 61 and the bottom end of the air outlet tube 62 are arranged with a height offset, combined with the height size of the culture bottle 4 and the height of the culture liquid, to achieve better ventilation efficiency.

[0044] In an embodiment of the present invention, optionally, Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 As shown, the ventilation tube 6 vertically penetrates the cover 5, and the part that passes through the top of the cover 5 upwards is designed as a hose structure, and the part that passes through the top of the cover 5 downwards is designed as a hard tube structure. When the cover 5 is folded upward, the hose part of the ventilation tube 6 is convenient to fold up, and the bottom end of the cover 5 is buckled on the top opening of the culture bottle 4. Regardless of whether the opening of the culture bottle 4 is sealed with a sterilization sealing film, the hard tube part of the ventilation tube 6 can be inserted into the culture bottle 4 for efficient ventilation.

[0045] In an embodiment of the present invention, optionally, Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 As shown, the longitudinal rope 8 is located below the transverse rope 7, and the longitudinal rope 8 is slidably connected to the limit block 9. The four corners of the edge of the limit block 9 are vertically provided with limit pins 10, and the limit pins 10 are symmetrically arranged on the front and back sides of the limit block 9. The front and back sides of the limit block 9 are respectively provided with a first monitoring component 11 and a second monitoring component 12. When the culture bottle 4 is placed on the oscillation table 3 along the gap between the transverse rope 7 and the longitudinal rope 8, the transverse rope 7 is pulled upward and inserted into the strip groove 51, which is used to elastically press downward against the top of the culture bottle 4. By sliding the limit block 9, the limit pin 10 is pressed against the outside of the culture bottle 4, and the first monitoring component 11 and the second monitoring component 12 are respectively facing the two sides of the culture bottle 4. Among them, the first monitoring component 11 and the second monitoring component 12 are one or more of a temperature sensor, a camera, and a humidity sensor. Therefore, the limit block 9 is pressed against the outer sides of the culture bottle 4, and is used to limit and fix the culture bottle 4. At the same time, the monitoring components on the limit block 9 are used to monitor the fermentation culture status in the bottle in real time, thereby achieving a better monitoring effect.

[0046] In an embodiment of the present invention, optionally, Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8As shown, a strip flange 91 is provided in parallel in the middle of one side of the limit block 9, and a threading hole 90 for the longitudinal rope 8 to pass through is provided in parallel in the middle of the strip flange 91, so that each limit block 9 is passed through the threading hole 90 and slidably connected to each longitudinal rope 8. When in use, the limit block 9 is manually slid so that the limit pin 10 is pressed against the outside of the culture bottle 4, and the limit block 9 can be freely rotated axially so that the monitoring parts on different surfaces face the culture bottle 4, thereby achieving the purpose of monitoring the specified fermentation process parameters.

[0047] In an embodiment of the present invention, optionally, Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 As shown, pin barrels 13 are provided on the four corners of the edge of the limit block 9. The pin barrels 13 are arranged perpendicular to the limit block 9 and are designed with open ends. More preferably, the pin barrel 13 is designed to pass through the limit block 9, and the limit pin 10 is elastically passed through the pin barrel 13, and the end of the limit pin 10 passes through the opening of the pin barrel 13. Specifically, for example, the middle end of the limit pin 10 can be connected to the limit pin 10 through existing conventional elastic components such as elastic ropes and springs, so that the limit pin 10 can elastically slide along the pin barrel 13 and be elastically pressed against the outside of the culture bottle 4.

[0048] The present invention also provides a fermentation process for fermenting red yeast rice using a purple Monascus strain, comprising the following steps:

[0049] Prepare a strain activation solution, inoculate the Monascus purpurogenum strain on a strain culture medium for culture activation, wash off the bacterial spores on the strain culture medium with sterile physiological saline, break up the spore aggregates and make them uniform, and obtain a spore suspension;

[0050] The spore suspension was inoculated into a culture bottle 4 containing a liquid seed culture medium, and the culture bottle 4 was placed on an oscillation table 3. The culture was shaken and cultured at 30° C. and 180 rpm for 48 hours to obtain an activated solution of the Monascus purpurogenum strain.

[0051] Under sterile conditions, the activation solution of Monascus purpurogenum strain was inoculated into the culture bottle 4 containing the culture medium, and fermented. Figure 12As shown, by pulling out the cover 5 downward and buckling it on the top opening of the culture bottle 4, the horizontal rope 7 is pulled up and inserted into the strip groove 51, which is used to elastically press downward against the top of the culture bottle 4, and slide the limit block 9 on the longitudinal rope 8 so that the limit pins 10 on the four corners of the edge of the limit block 9 are elastically pressed against the outside of the culture bottle 4. The first monitoring member 11 and the second monitoring member 12 on the front and back of the limit block 9 face the two sides of the culture bottle 4 respectively, which are used to monitor the fermentation culture status in the bottle in real time. The fermentation culture adopts variable temperature culture, and the variable temperature culture conditions are as follows: after culturing at 30-32°C for 3-5 days, it is cultured at 25-27°C for 15-17 days, and the humidity is 60%-75%. The culture bottle 4 is ventilated through the ventilation pipe 6, and the ventilation time is 30 minutes per day. After the variable temperature culture is completed, the culture medium is dried at 40-45°C to obtain the final red yeast rice product.

[0052] The functional red yeast rice product obtained by the present invention meets the quality standard requirements of red yeast rice under the item of Xuezhikang in the pharmacopoeia after testing, and can be used as the raw material medicine of the traditional Chinese medicine Xuezhikang and a health functional food.

[0053] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention is limited to these examples. Within the scope of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

Claims

1. A culture vessel for fermenting red yeast rice using a purple Monascus strain, comprising a vertical culture vessel body (1), characterized in that: The vertical incubator body (1) is provided with an incubator (2), and an oscillating table (3) is provided at the inner bottom end of the incubator (2). The oscillating table (3) is designed as a groove structure with an open top for placing a culture bottle (4), and the culture bottle (4) is designed as a top open; The inner top of the incubator (2) is movably connected to a plurality of covers (5), the top of each cover (5) is connected to a ventilation tube (6), the bottom of each cover (5) is flared, and the top of each cover (5) is provided with a strip groove (51); A plurality of transverse ropes (7) are arranged in parallel along the horizontal direction on the inner side of the oscillation table (3), and the two ends of the transverse ropes (7) are elastically connected to the inner side of the oscillation table (3). A plurality of longitudinal ropes (8) are arranged in parallel along the horizontal direction on the inner side of the oscillation table (3), and the two ends of the longitudinal ropes (8) are elastically connected to the inner side of the oscillation table (3). The length direction of the longitudinal ropes (8) is perpendicular to the length direction of the transverse ropes (7). When a culture bottle (4) is placed in the gap between the transverse ropes (7) and the longitudinal ropes (8), the gap between the transverse ropes (7) and the longitudinal ropes (8) is used to elastically press against the outer side of the culture bottle (4). When the cover (5) is pulled out downward, so that the bottom end of the cover (5) is buckled on the top opening of the culture bottle (4), at least one of the transverse ropes (7) and the longitudinal ropes (8) is passed through the strip groove (51) to elastically press against the top of the culture bottle (4) downward.

2. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 1, characterized in that: The ventilation pipe (6) comprises an air inlet pipe (61) located on one side of the cover (5), and an air outlet pipe (62) located on the other side of the cover (5), wherein the bottom end of the air inlet pipe (61) and the bottom end of the air outlet pipe (62) are arranged in a height-staggered manner.

3. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 1, characterized in that: The ventilation pipe (6) vertically penetrates the cover (5), and the portion passing through the top of the cover (5) upwards is designed as a hose structure, and the portion passing through the top of the cover (5) downwards is designed as a hard tube structure.

4. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 1, characterized in that: The longitudinal rope (8) is located below the transverse rope (7), and a limiting block (9) is slidably connected to the longitudinal rope (8). Limiting pins (10) are vertically provided on the four corners of the edge of the limiting block (9). A first monitoring component (11) and a second monitoring component (12) are respectively provided on the front and back surfaces of the limiting block (9). When the culture bottle (4) is placed on the oscillation table (3) along the gap between the transverse rope (7) and the longitudinal rope (8), the limiting block (9) is slid so that the limiting pin (10) is pressed against the outside of the culture bottle (4). The first monitoring component (11) and the second monitoring component (12) are respectively facing the two sides of the culture bottle (4) to monitor the fermentation culture status in the bottle.

5. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 4, characterized in that: A strip flange (91) is provided in parallel in the middle of one side of the limit block (9), and a threading hole (90) for the longitudinal rope (8) to pass through is provided in parallel in the middle of the strip flange (91).

6. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 4, characterized in that: Pin barrels (13) are provided on the four corners of the edge of the limit block (9). The pin barrels (13) are arranged perpendicular to the limit block (9) and are designed with open ends. The limit pin (10) is elastically inserted into the pin barrel (13), and the end of the limit pin (10) is inserted along the opening of the pin barrel (13).

7. A culture vessel for fermenting red yeast rice using a purple Monascus strain according to claim 6, characterized in that: The first monitoring component (11) and the second monitoring component (12) adopt one or more of a temperature sensor, a camera, and a humidity sensor.

8. A fermentation process for fermenting red yeast rice using a purple Monascus strain, wherein the fermentation is carried out using a culture vessel for fermenting red yeast rice using a purple Monascus strain as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: Prepare a strain activation solution, inoculate the Monascus purpurogenum strain on a strain culture medium for culture activation, wash off the bacterial spores on the strain culture medium with sterile physiological saline, break up the spore aggregates and make them uniform, and obtain a spore suspension; The spore suspension is inoculated into a culture bottle (4) containing a liquid seed culture medium, the culture bottle (4) is placed on an oscillation table (3), and oscillated for 48 hours at 30° C. and 180 rpm to obtain an activated solution of the Monascus purpurogenum strain; Under aseptic conditions, a culture bottle (4) containing a culture matrix is filled with an activation liquid of the Monascus purpureus strain for fermentation and culture. The cover (5) is pulled out downward and buckled onto the top opening of the culture bottle (4). The transverse rope (7) is pulled upward and inserted into the strip groove (51) to elastically press against the top of the culture bottle (4) downward, and the limit block (9) on the longitudinal rope (8) is slid so that the limit pins (10) on the four corners of the limit block (9) elastically press against the outside of the culture bottle (4). The first monitoring device on the front and back sides of the limit block (9) is used to detect the presence of the virus. The first monitoring element (11) and the second monitoring element (12) face the two sides of the culture bottle (4) respectively, and are used to monitor the fermentation culture status in the bottle in real time. The fermentation culture adopts variable temperature culture, and the variable temperature culture conditions are as follows: after culturing at 30-32°C for 3-5 days, it is cultured at 25-27°C for 15-17 days, and the humidity is 60%-75%. The culture bottle (4) is ventilated through the ventilation tube (6), and the ventilation time is 30 minutes per day. After the variable temperature culture is completed, the culture medium is dried at 40-45°C to obtain the final red yeast rice product.

9. Use of a finished red yeast rice product prepared by the fermentation process of red yeast rice with the purple Monascus strain according to claim 8 in the preparation of lipid-lowering drugs and health functional foods.

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