Indel marker EH-03-Indel-108 highly closely linked with corncob position and application of Indel marker EH-03-Indel-108

By developing the Indel marker EH-03-Indel-108 with high and tight linkages at corn ears, the difficulties in identifying and breeding of high traits in corn ears in the prior art have been solved, accurate identification and early screening have been achieved, and breeding efficiency of lodging-resistant varieties has been improved, and operations have been simplified and costs have been reduced.

CN120485412AActive Publication Date: 2025-08-15AGRICULTURAL GENOMICS INSTITUTE AT SHENZHEN CHINESE ACADEMY OF AGRICULTURAL SCIENCES (SHENZHEN BRANCH GUANGDONG LABORATORY FOR LINGNAN MODERN AGRICULTURE)
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Patent Information

Application Number
CN202510602197.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-12
Publication Date
2025-08-15
Estimated Expiration
2045-05-12

AI Technical Summary

Technical Problem

The prior art has problems such as biosafety risks, long breeding cycle, high detection cost, low throughput and lack of marking in gene editing and molecular marker assisted breeding with high ear positions, making it difficult to achieve accurate identification and early screening of high ear positions, limiting the breeding efficiency of resistant lodging varieties.

Method used

An Indel tag EH-03-Indel-108, which is closely linked to corn ear high, is located in a specific region of corn chromosome 3. It uses specific primer pair PCR amplification and agarose gel electrophoresis to quickly identify the ear high haplotype of corn inbred lines, providing low ear variety screening and anti-lost breeding support.

Benefits of technology

The accurate identification of high traits of corn ears has been achieved, the breeding efficiency of lodging-resistant varieties has been improved, the operation process has been simplified, the cost has been reduced, the genetic improvement of corn and the selectivity of breeding materials has been promoted, and the breeding cycle has been shortened.

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Abstract

The invention relates to the technical field of plant molecular breeding, in particular to an Indel marker EH-03-Internel-108 highly closely linked with a corn ear position and application of the Indel marker EH-03-Internel-108 highly closely linked with the corn ear position, and the marker is located in a specific region of a third chromosome of corn and is a 4bp insertion / deletion polymorphism marker. Through a specific primer pair, PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis, the ear position haplotype can be judged according to a 216bp band. The molecular marker is widely applied to corn molecular marker-assisted breeding, and low-ear lodging-resistant varieties can be screened. Compared with the prior art, the method has the advantages that the corn ear height character can be accurately identified, the lodging-resistant variety breeding efficiency is improved, the operation is simple and convenient, the cost is low, the corn genetic improvement is promoted, the breeding material selectivity is enhanced, and the method has important theoretical and practical significance on the molecular mechanism research and genetic breeding improvement process of the corn ear height character.
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