Soybean extract as well as extraction method and application thereof

Abstract

The invention relates to the technical field of biology, and discloses a soybean extract as well as an extraction method and application thereof. The extraction method of the soybean extract comprises the following steps: performing circulating freezing and thawing treatment on soybeans to obtain pretreated soybeans; putting the pretreated soybeans into a microwave reaction kettle, and carrying out primary microwave extraction; after the primary microwave extraction is finished, condensing gas in the microwave reaction kettle to obtain a soybean extract A; crushing soybeans, and mixing the crushed soybeans with deionized water to obtain soybean slurry; placing the soybean slurry in a microwave reaction kettle, introducing nitrogen into the microwave reaction kettle, and carrying out secondary microwave extraction; filtering the soybean slurry subjected to the secondary microwave extraction by using a ceramic membrane, and freeze-drying to obtain a soybean extract B; and mixing the soybean extract A and the soybean extract B to obtain the soybean extract. The treatment effective rates of the soybean extract on gastric ulcer and gastritis can reach 90.0% and 80.0% respectively, and the soybean extract has good gastric ulcer and gastritis treatment effects.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a soybean extract and an extraction method and application thereof. Background Art

[0002] Gastric ulcers and gastritis are common digestive system diseases with complex pathogenesis, linked to multiple factors including Helicobacter pylori infection, medications, alcohol, and stress. Currently, the drugs used clinically to treat gastric ulcers and gastritis primarily include acid suppressants, gastric mucosal protectants, and antibiotics. While these medications can alleviate symptoms and promote ulcer healing to a certain extent, they also have some drawbacks.

[0003] Research on the application of soy extracts in the treatment of gastric ulcers and gastritis has made some progress. Studies have shown that soy isoflavones can exert anti-ulcer effects by inhibiting the release of inflammatory factors and promoting the proliferation of gastric mucosal cells. Soy saponins can protect the gastric mucosa and inhibit the growth of Helicobacter pylori. However, existing soy extract extraction methods suffer from low extraction efficiency and severe loss of active ingredients, limiting their clinical application.

[0004] In view of this, developing a soybean extract and an extraction method thereof has important practical significance for the treatment of gastric ulcer and gastritis. Summary of the Invention

[0005] The purpose of the present invention is to provide a soybean extract and an extraction method and application thereof.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention:

[0008] A method for extracting soybean extract comprises the following steps:

[0009] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0010] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor and performing a single microwave extraction;

[0011] 3) After the microwave extraction in step 2) is completed, condensing the gas in the microwave reactor to obtain soybean extract A;

[0012] 4) grinding the soybeans obtained after the microwave extraction in step 2), and then mixing the soybeans with deionized water to moisten the soybeans to obtain a soybean slurry;

[0013] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, introducing nitrogen into the microwave reactor, and performing a second microwave extraction;

[0014] 6) filtering the soybean slurry after the second microwave extraction in step 5) using a ceramic membrane, and freeze-drying the filtrate to obtain soybean extract B;

[0015] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0016] Furthermore, in step 1), the freeze-thaw cycle treatment is specifically: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, and repeating the cycle 2 to 4 times.

[0017] Furthermore, in step 2), the primary microwave treatment is specifically as follows: controlling the microwave frequency to be 2-3 GHz, and performing a single microwave extraction for 30-90 seconds.

[0018] Furthermore, in step 3), the condensation is specifically: condensation at -5°C and -0.1 MPa.

[0019] Furthermore, in step 4), the pulverization is specifically: pulverizing to a particle size of 0.5 mm.

[0020] Furthermore, in step 4), the mass ratio of the soybeans to deionized water is 1:4.

[0021] Furthermore, in step 4), the material is moistened at 40° C. and 500 rpm for 10 to 20 minutes.

[0022] Furthermore, in step 5), the flow rate of the nitrogen is 5 L / min.

[0023] Furthermore, in step 5), the secondary microwave extraction is specifically as follows: controlling the microwave frequency to be 1-2 GHz, the magnetic field strength to be 150-200 mT, and the secondary microwave extraction to be 30-90 s.

[0024] Furthermore, in step 6), the freeze-drying is specifically: freeze-drying at -20°C and -0.1 MPa.

[0025] The second technical solution of the present invention:

[0026] The soybean extract is obtained by extracting the soybean extract using the above-mentioned soybean extract extraction method.

[0027] The third technical solution of the present invention:

[0028] Application of the soybean extract in medicines or health foods for treating gastric ulcers and gastritis.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The soybean extract provided by the present invention has an efficacy of up to 90.0% in treating gastric ulcers and has a good gastric ulcer treatment effect;

[0031] The soybean extract provided by the present invention has a therapeutic efficiency of up to 80.0% for gastritis and has a good gastritis therapeutic effect.

[0032] The present invention integrates the principles of microwave and plasma effects to effectively extract multiple active ingredients from soybeans. Specifically:

[0033] The present invention is based on the "microwave grape experiment." When soybeans are placed in a microwave magnetic field, the water molecules and other polar molecules in the soybeans, driven by the alternating electromagnetic field, undergo high-frequency rotation and friction, just like grapes in a microwave oven. This creates a synergistic effect of local thermal and non-thermal effects. As microwave energy accumulates, when the mechanical strength threshold of the soybean cell wall is exceeded, macromolecular chains such as proteins and polysaccharides break under the non-thermal effect, generating charged secondary metabolic small molecules such as amino acids and phenolic acids.

[0034] During microwave exposure, the soybean cell structure produces unique electromagnetic wave response characteristics due to the Mie scattering resonance effect. Adjacent soybeans are coupled and superimposed through evanescent waves at the interface, further amplifying the electromagnetic energy density. This amplified energy triggers the structural breakdown of macromolecules within the soybeans, causing macromolecules such as proteins and polysaccharides to ionize or depolymerize, converting them into charged secondary metabolite molecules. Under the action of negative pressure, these secondary metabolite molecules migrate directionally to the condensation system and are ultimately recovered as soybean extract. DETAILED DESCRIPTION

[0035] Various exemplary embodiments of the present invention are now described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terms used in the present invention are only for describing specific embodiments and are not intended to limit the present invention.

[0036] In addition, for numerical ranges in the present invention, it is understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in the stated range is also included in the present invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.

[0037] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0038] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0039] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0040] In the following examples, a method for extracting soybean extract comprises the following steps:

[0041] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0042] The freeze-thaw cycle treatment is specifically as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, and repeating for 2 to 4 times;

[0043] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 2-3 GHz, and performing microwave extraction for 30-90 seconds at a time;

[0044] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0045] 4) grinding the soybeans obtained after the microwave extraction in step 2) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 10-20 minutes to obtain a soybean slurry;

[0046] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1-2 GHz, the magnetic field strength to 150-200 mT, and performing a secondary microwave extraction for 30-90 s;

[0047] 6) filtering the soybean slurry after the second microwave extraction in step 5) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0048] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0049] Example 1

[0050] A soybean extract

[0051] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0052] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, for 2 cycles;

[0053] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 2 GHz, and performing microwave extraction for 30 seconds at a time;

[0054] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0055] 4) grinding the soybeans obtained after the microwave extraction in step 2) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 10 minutes to obtain a soybean slurry;

[0056] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1 GHz, the magnetic field intensity to 150 mT, and performing a second microwave extraction for 30 s;

[0057] 6) filtering the soybean slurry after the second microwave extraction in step 5) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0058] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0059] Example 2

[0060] A soybean extract

[0061] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0062] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, for 3 cycles;

[0063] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 2.45 GHz, and performing microwave extraction for 60 seconds at a time;

[0064] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0065] 4) grinding the soybeans obtained after the microwave extraction in step 2) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 15 minutes to obtain a soybean slurry;

[0066] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1.8 GHz, the magnetic field intensity to 180 mT, and performing a secondary microwave extraction for 45 s;

[0067] 6) filtering the soybean slurry after the second microwave extraction in step 5) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0068] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0069] Example 3

[0070] A soybean extract

[0071] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0072] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, and repeating the cycle 4 times;

[0073] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 3 GHz, and performing microwave extraction for 90 seconds at a time;

[0074] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0075] 4) grinding the soybeans obtained after the microwave extraction in step 2) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 20 minutes to obtain a soybean slurry;

[0076] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 2 GHz, the magnetic field intensity to 200 mT, and performing a secondary microwave extraction for 90 s;

[0077] 6) filtering the soybean slurry after the second microwave extraction in step 5) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0078] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0079] Comparative Example 1

[0080] A soybean extract

[0081] 1) Place soybeans in a microwave reactor, control the microwave frequency to 2.45 GHz, and perform microwave extraction for 60 seconds at a time;

[0082] 2) After the microwave extraction in step 1) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0083] 3) grinding the soybeans after the microwave extraction in step 1) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 15 minutes to obtain a soybean slurry;

[0084] 4) placing the soybean slurry obtained in step 2) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1.8 GHz, the magnetic field intensity to 180 mT, and performing a second microwave extraction for 45 s;

[0085] 5) filtering the soybean slurry after the second microwave extraction in step 4) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0086] 6) The soybean extract A obtained in step 2) and the soybean extract B obtained in step 5) are mixed to obtain the soybean extract.

[0087] Comparative Example 2

[0088] A soybean extract

[0089] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0090] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, for 3 cycles;

[0091] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 2.45 GHz, and performing microwave extraction for 60 seconds;

[0092] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain the soybean extract.

[0093] Comparative Example 3

[0094] A soybean extract

[0095] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0096] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, for 3 cycles;

[0097] 2) grinding the pretreated soybeans obtained in step 1) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and simmering the mixture at 40° C. and 500 rpm for 15 minutes to obtain a soybean slurry;

[0098] 3) placing the soybean slurry obtained in step 2) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1.8 GHz, the magnetic field intensity to 180 mT, and performing microwave extraction for 45 s;

[0099] 4) The soybean slurry after microwave extraction in step 3) was filtered using a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20°C and -0.1 MPa to remove water, thereby obtaining the soybean extract.

[0100] Comparative Example 4

[0101] A soybean extract

[0102] 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans;

[0103] The freeze-thaw cycle treatment is as follows: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, for 3 cycles;

[0104] 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor, controlling the microwave frequency to 2.45 GHz, and performing microwave extraction for 60 seconds at a time;

[0105] 3) After the microwave extraction in step 2) is completed, the gas in the microwave reactor is condensed at -5°C and -0.1 MPa to obtain soybean extract A;

[0106] 4) grinding the soybeans obtained after the microwave extraction in step 2) to a particle size of 0.5 mm, then mixing the soybeans with deionized water in a mass ratio of 1:4, and heating the mixture at 40° C. and 500 rpm for 15 minutes to obtain a soybean slurry;

[0107] 5) placing the soybean slurry obtained in step 4) in a microwave reactor, controlling the nitrogen flow rate to 5 L / min, introducing nitrogen into the microwave reactor, then controlling the microwave frequency to 1.8 GHz, and performing a second microwave extraction for 45 s;

[0108] 6) filtering the soybean slurry after the second microwave extraction in step 5) through a 0.1 μm ceramic membrane, and freeze-drying the filtrate at -20°C and -0.1 MPa to remove water, thereby obtaining soybean extract B;

[0109] 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

[0110] Effect verification

[0111] 1. Safety Inspection

[0112] 80 healthy mice weighing 20-23 g, half male and half female, were selected and acclimatized in a standard animal room for 7 days before the experiment (temperature 22±2°C, humidity 50±10%, 12 h light / dark cycle, free access to food and water). They were randomly divided into 8 groups, 10 mice in each group, and gavage-administered the soybean extracts prepared in Examples 1-3 and Comparative Examples 1-4 or normal saline (5 mL / (kg·day)). The experiment lasted for 6 months, during which the activity and mortality of the mice were observed.

[0113] After testing, there was no significant difference in the activity status of mice in each group, and no death occurred, which shows that the soybean extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 4 of the present invention have high safety and can be used for subsequent testing of the therapeutic effects of gastric ulcers and gastritis.

[0114] 2. Gastric ulcer treatment effect test

[0115] Sample selection: 240 patients with gastric ulcer without other diseases were divided into 8 groups;

[0116] Treatment plan: Orally take the soybean extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 4, 30 mL each time, twice a day for 2 consecutive months, during which time all spleen-strengthening and stomach-nourishing drugs are discontinued;

[0117] Control treatment: Omeprazole enteric-coated capsules, taken orally, once a day, 20 mg each time, for 2 consecutive months, during which time all other spleen-tonifying and stomach-nourishing drugs were discontinued;

[0118] Efficacy criteria:

[0119] Cure: All symptoms disappear, ulcer scars heal or heal without scars, and Helicobacter pylori is eradicated;

[0120] Markedly effective: The main symptoms disappear, the ulcer reaches the healing stage (H2), and Helicobacter pylori is eradicated;

[0121] Effective: Symptoms are alleviated, ulcers reach the healing stage (H1), and Helicobacter pylori is eradicated and reduced;

[0122] Ineffective: no improvement in symptoms, endoscopy, and Helicobacter pylori examination;

[0123] Effective rate (%) = (number of cured cases + number of markedly effective cases + number of effective cases) / total number of cases × 100%;

[0124] The results of the test on the therapeutic effect of gastric ulcer are shown in Table 1;

[0125] Table 1 Gastric ulcer treatment effect test results

[0126]

[0127] As can be seen from the data in Table 1, the soybean extract provided by the present invention has a therapeutic efficiency of up to 90.0% for gastric ulcer, and has a good gastric ulcer therapeutic effect.

[0128] 3. Test of the therapeutic effect of gastritis

[0129] Sample selection: 240 patients with chronic superficial gastritis without other diseases were divided into 8 groups;

[0130] Treatment plan: Orally take the soybean extracts prepared in Examples 1 to 3 and Comparative Examples 1 to 4, 30 mL each time, twice a day, for 1 month, during which time all spleen-strengthening and stomach-nourishing drugs are discontinued;

[0131] Control treatment: Oral hydrotalcite tablets, 10 mg twice a day for 1 month, during which all other spleen-tonifying and stomach-nourishing drugs were discontinued.

[0132] Efficacy criteria:

[0133] Cure: No obvious abnormality in gastric mucosal pathology;

[0134] Markedly effective: gastric mucosal pathology score decreased by 2 grades;

[0135] Effective: gastric mucosal pathology score decreased by 1 grade;

[0136] Ineffective: The degree of gastric mucosal inflammation remains unchanged or worsens;

[0137] Effective rate (%) = (number of cured cases + number of markedly effective cases + number of effective cases) / total number of cases × 100%;

[0138] The results of the gastritis treatment effect test are shown in Table 2;

[0139] Table 2 Gastritis treatment effect test results

[0140]

[0141] As can be seen from the data in Table 2, the soybean extract provided by the present invention has an effective rate of up to 80.0% in treating gastritis, and has a good effect in treating gastritis.

[0142] The soybean extract prepared in Example 2 was subjected to extensive targeted metabolomics testing, and a total of 656 metabolites were detected, as shown in Table 3.

[0143] Table 3 Metabolites

[0144]

[0145]

[0146]

[0147]

[0148]

[0149]

[0150]

[0151]

[0152]

[0153]

[0154]

[0155] The soybean extract prepared in Example 2 was tested for water cluster size;

[0156] After testing, the water molecule cluster size of the soybean extract prepared in Example 2 was 38.81 Hz.

[0157] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered by the scope of protection of the claims of the present invention.

Claims

1. A method for extracting soybean extract, characterized in that: The following steps are involved: 1) performing a freeze-thaw cycle on soybeans to obtain pretreated soybeans; 2) placing the pretreated soybeans obtained in step 1) in a microwave reactor and performing a single microwave extraction; 3) After the microwave extraction in step 2) is completed, condensing the gas in the microwave reactor to obtain soybean extract A; 4) grinding the soybeans obtained after the microwave extraction in step 2), and then mixing the soybeans with deionized water to moisten the soybeans to obtain a soybean slurry; 5) placing the soybean slurry obtained in step 4) in a microwave reactor, introducing nitrogen into the microwave reactor, and performing a second microwave extraction; 6) filtering the soybean slurry after the second microwave extraction in step 5) using a ceramic membrane, and freeze-drying the filtrate to obtain soybean extract B; 7) The soybean extract A obtained in step 3) and the soybean extract B obtained in step 6) are mixed to obtain the soybean extract.

2. The method for extracting soybean extract according to claim 1, wherein: In step 1), the freeze-thaw cycle treatment is specifically: freezing at -40°C for 1 hour, then thawing at 25°C for 0.5 hour, and repeating the cycle 2 to 4 times.

3. The method for extracting soybean extract according to claim 1, wherein: In step 2), the microwave extraction is specifically performed by controlling the microwave frequency to be 2 to 3 GHz and performing microwave extraction for 30 to 90 seconds.

4. The method for extracting soybean extract according to claim 1, wherein In step 3), the condensation is specifically: condensation at -5°C and -0.1 MPa.

5. The method for extracting soybean extract according to claim 1, wherein: In step 4), the crushing is specifically performed as follows: crushing to a particle size of 0.5 mm; the mass ratio of the soybeans to deionized water is 1:4; and the moistening is specifically performed as follows: moistening the material at 40° C. and 500 rpm for 10 to 20 minutes.

6. The method for extracting soybean extract according to claim 1, wherein: In step 5), the flow rate of the nitrogen is 5 L / min; the secondary microwave extraction is specifically as follows: controlling the microwave frequency to be 1-2 GHz, the magnetic field strength to be 150-200 mT, and the secondary microwave extraction to be 30-90 s.

7. The method for extracting soybean extract according to claim 1, characterized in that: In step 6), the freeze drying is specifically: freeze drying at -20°C and -0.1 MPa.

8. A soybean extract obtained by the method for extracting soybean extract according to any one of claims 1 to 7.

9. Use of the soybean extract according to claim 8 in medicines or health foods for treating gastric ulcers and gastritis.

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