Evaporation compensation device

Abstract

The evaporation compensation device comprises a liquid storage plate cover integrally formed by a middle plate, an upper edge outer wall and a lower edge outer wall, the surface of the middle plate is connected with a plurality of circular protruding rings arranged on the inner side portion of the lower edge outer wall and a plurality of protruding columns arranged on the inner side portion of the upper edge outer wall, and the protruding columns are designed in an annular stepped mode from the periphery to the inner circle. The surface of the middle plate is provided with a plurality of cavities corresponding to the circular protruding rings and the protruding columns, and the cavities are concentric with the circular protruding rings and the protruding columns respectively and correspond to the circular protruding rings and the protruding columns. According to the liquid storage plate, a honeycomb-like liquid storage plate cover can be formed through the honeycomb-like matrix structure, the airflow notch design, the circular protruding ring structure and the flow limiting baffle, so that when the liquid storage plate cover is used in cooperation with the liquid storage plate, the statistical percentages of the evaporation capacities of all plate holes of the liquid storage plate can be made to be the same, and the problem of the edge evaporation effect of the liquid storage plate is effectively solved; the uniformity of experimental conditions of each liquid storage plate hole is improved, and the repeatability of experimental results is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to an evaporation compensation device. Background Art

[0002] In the field of biomedical immunology experiments, multi-well reservoir plates (culture plates) are widely used due to their high throughput and ease of use. Currently, multi-well reservoir plates come in 6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 1536-well, and even larger formats. The 96-well reservoir plate, with its moderate sample throughput and ease of use, is the most frequently used type. Depending on experimental needs, reservoir plates are further subdivided into deep-well plates for molecular experiments, ELISA plates for enzyme-linked immunosorbent assays, and cell culture plates for cell culture experiments.

[0003] Currently, cell culture experiments typically require a constant temperature incubation period of 4-5 days. During this period, the culture medium requires a certain amount of gas exchange with the external environment, and cellular metabolism generates heat, which causes significant evaporation of the solution from each well of the reservoir plate. During this evaporation process, due to temperature differences between the center and edge wells of the plate, as well as varying air velocity above the liquid surface of each well, the edge wells of the plate experience greater evaporation than the central wells, commonly known as the "edge evaporation effect." The corners and outermost wells of the plate experience significantly greater evaporation than the central wells due to faster air velocity. This variability not only leads to an uneven cell growth environment, causing experimental errors and even failure, but also can lead to inconsistent molar concentrations of solution substances in other experiments (such as enzyme-linked immunosorbent assays). Furthermore, in other experiments (such as enzyme-linked immunosorbent assays), this difference in evaporation between different wells can cause anomalies in the curves of parallel comparisons, seriously compromising the reliability of experimental results.

[0004] It is understood that the current market solution to the aforementioned "edge evaporation effect" problem is to reduce the aperture of the reservoir plate or add buffer between the pores to increase the saturated water vapor pressure at the gas temperature above the liquid in each reservoir, thereby reducing the difference in evaporation. However, in actual operation, this solution is not only cumbersome to add buffer, but the reservoir plate is also highly susceptible to contamination during the frequent shaking and mixing process, significantly increasing the workload and experimental risks of the experimenter. To this end, the present invention proposes an evaporation compensation device. Summary of the Invention

[0005] The present invention provides an evaporation compensation device to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is: The top of the lid is closed by a spring, and the lid is closed by a spring, and the lid is closed by a spring. The lid is closed by a spring, and the lid is closed by a spring. The lid is closed by a spring.

[0007] Preferably, the simulated honeycomb matrix includes simulated honeycomb holes A, simulated honeycomb holes B, simulated honeycomb holes C, simulated honeycomb holes D, simulated honeycomb holes E and simulated honeycomb holes F, and the simulated honeycomb holes A, simulated honeycomb holes B, simulated honeycomb holes C, simulated honeycomb holes D, simulated honeycomb holes E and simulated honeycomb holes F are respectively provided with 2n numbers, wherein n≥2, and several of the simulated honeycomb holes A are located at the four corners of the outer edge of the simulated honeycomb matrix, several of the simulated honeycomb holes F are located at the center of the simulated honeycomb matrix, several of the simulated honeycomb holes E are divided into two groups and are symmetrically arranged with the simulated honeycomb holes F as the symmetry axis, several of the simulated honeycomb holes D form rectangular frames and are distributed around the simulated honeycomb holes E and the simulated honeycomb holes F, several of the simulated honeycomb holes C form rectangular frames and are distributed around the simulated honeycomb holes D, several of the simulated honeycomb holes B are distributed around the simulated honeycomb holes C, and form rectangular frames with several of the simulated honeycomb holes A.

[0008] Preferably, the height of the convex column corresponding to the imitation honeycomb hole B in the imitation honeycomb matrix is ​​smaller than the height of the convex column corresponding to the imitation honeycomb hole C in the imitation honeycomb matrix, the height of the convex column corresponding to the imitation honeycomb hole C in the imitation honeycomb matrix is ​​smaller than the height of the convex column corresponding to the imitation honeycomb hole D in the imitation honeycomb matrix, the height of the convex column corresponding to the imitation honeycomb hole D in the imitation honeycomb matrix is ​​smaller than the height of the convex column corresponding to the imitation honeycomb hole E in the imitation honeycomb matrix, and the height of the convex column corresponding to the imitation honeycomb hole E in the imitation honeycomb matrix is ​​smaller than the height of the convex column corresponding to the imitation honeycomb hole F in the imitation honeycomb matrix.

[0009] Preferably, the convex columns are distributed in a rectangular array, and a matrix gap is provided between every two adjacent convex columns.

[0010] Preferably, the simulated honeycomb holes A located at the four corners of the outer edge of the simulated honeycomb matrix are formed by circular raised rings, and the flow limiting baffles at the four corners of the simulated honeycomb matrix are respectively fixedly connected to the outer walls of the corresponding circular raised rings.

[0011] Preferably, the circular raised rings are distributed in a rectangular array, and the cavity volume of the imitation honeycomb hole A in the imitation honeycomb matrix is ​​smaller than the cavity volume of the imitation honeycomb hole B in the imitation honeycomb matrix, the cavity volume of the imitation honeycomb hole B in the imitation honeycomb matrix is ​​smaller than the cavity volume of the imitation honeycomb hole C in the imitation honeycomb matrix, the cavity volume of the imitation honeycomb hole C in the imitation honeycomb matrix is ​​smaller than the cavity volume of the imitation honeycomb hole D in the imitation honeycomb matrix, the cavity volume of the imitation honeycomb hole D in the imitation honeycomb matrix is ​​smaller than the cavity volume of the imitation honeycomb hole E in the imitation honeycomb matrix, and the cavity volume of the imitation honeycomb hole E in the imitation honeycomb matrix is ​​smaller than the cavity volume of the imitation honeycomb hole F in the imitation honeycomb matrix.

[0012] Preferably, the air flow slot includes a first slot and a second slot that is perpendicular to the first slot, and there are two of each of the first slot and the second slot, and the two first slots are arranged parallel to each other and are correspondingly arranged on the two side surfaces of the upper outer wall, and the two second slots are arranged parallel to each other and are correspondingly arranged on the other two side surfaces of the upper outer wall.

[0013] Preferably, the first notch and the second notch are respectively arc-shaped, and the centers of the two first notches are respectively on the same vertical line with the center points of the corresponding side surfaces of the upper outer wall, and the centers of the two second notches are respectively on the same vertical line with the center points of the corresponding side surfaces of the upper outer wall.

[0014] Preferably, the inner side wall of the lower outer wall is provided with three first breathable arc-shaped protrusions connected to the middle plate and one second breathable arc-shaped protrusion connected to the middle plate, and the three first breathable arc-shaped protrusions and one second breathable arc-shaped protrusion are respectively provided at the four corners of the middle plate and are respectively connected to the inner side wall of the lower outer wall.

[0015] Preferably, an evaporation compensation device further includes a plurality of anti-pollution raised rings connected to the surface of the liquid storage plate, and the plurality of anti-pollution raised rings correspond one-to-one to the plurality of liquid storage plate holes, and the anti-pollution raised rings are used to interference fit with the circular raised ring.

[0016] By adopting the above technical solution, the beneficial effects achieved by the present invention are as follows: The present invention forms a series of cylindrical structures with staggered heights and annular symmetry according to the number of liquid storage plate holes through the simulated honeycomb matrix structure on the liquid storage plate cover, so that the liquid storage plate cover can achieve a gradient thermal insulation function, thereby realizing temperature compensation for the liquid storage plate holes.

[0017] The present invention achieves compensation adjustment of the evaporation percentage in each liquid storage plate hole in the liquid storage plate by making the volume of the central honeycomb hole of the simulated honeycomb matrix on the liquid storage plate cover larger than the volume of the edge honeycomb holes, thereby realizing the evaporation compensation function of the liquid storage plate holes.

[0018] The present invention greatly reduces the air flow rate above the liquid storage plate holes through the mutual interference fit of the circular raised ring and the anti-pollution raised ring compared to the traditional liquid storage plate and liquid storage plate cover design, thereby reducing the overall evaporation amount of the liquid storage plate holes, thereby narrowing the difference in evaporation amount of each liquid storage plate hole and reducing the edge evaporation effect.

[0019] The present invention can effectively reduce the air flow of the liquid storage plate holes corresponding to the imitation honeycomb holes A at the four corners of the liquid storage plate cover by adding four flow limiting baffles, ensure that the air flow above the liquid storage plate holes corresponding to the four corners of the liquid storage plate cover is consistent with that of other liquid storage plate holes, and effectively reduce the evaporation amount of the liquid storage plate holes corresponding to the imitation honeycomb holes A.

[0020] When the liquid storage plates are stacked, the present invention can gradually increase the air flow rate at the center of the upper part of the liquid storage plate cover and gradually reduce the air flow rate at the four corners of the liquid storage plate through the first notch and the second notch designed on the upper outer wall, thereby achieving temperature compensation; and, through the matrix gap design between every two convex columns in the honeycomb matrix, it can be ensured that the honeycomb structures above the liquid storage plate cover are independent of each other, ensuring smooth air flow, which is conducive to the average diffusion and conduction of heat.

[0021] In summary, through the imitation honeycomb matrix structure, airflow slot design, circular raised ring structure and the setting of the flow limiting baffle, the present invention can form a honeycomb-shaped liquid storage plate cover, so that when used in conjunction with the liquid storage plate, the statistical percentage of evaporation amount of each plate hole of the liquid storage plate can be made the same, effectively solving the "edge evaporation effect" problem of the 96-well plate, improving the uniformity of the experimental conditions of each liquid storage plate hole, and ensuring the repeatability of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the liquid storage plate cover of the present invention.

[0023] Figure 2 This is a schematic diagram of the top view of the liquid storage plate cover of the present invention.

[0024] Figure 3 This is a bottom view of the structure of the liquid storage plate cover of the present invention.

[0025] Figure 4 It is a bottom-up three-dimensional structural schematic diagram of the liquid storage plate cover of the present invention.

[0026] Figure 5 for Figure 4 Enlarged structural diagram at point A in the middle.

[0027] Figure 6 It is a schematic diagram of the front cross-sectional three-dimensional structure of the liquid storage plate cover of the present invention.

[0028] Figure 7 It is a schematic diagram of the front cross-sectional plan structure of the liquid storage plate cover of the present invention.

[0029] Figure 8 It is a schematic structural diagram of the liquid storage plate cover and the liquid storage plate of the present invention.

[0030] Figure 9 Schematic diagram of the distribution of liquid storage plate holes.

[0031] Figure 10 It is a schematic diagram showing the statistical percentage of evaporation amount of each well of the liquid storage plate after the present invention is used.

[0032] In the figure: 1, liquid storage plate cover; 101, middle plate; 102, upper outer wall; 103, lower outer wall; 2. Circular raised ring; 3. convex column; 4. Cavity; 5. Imitation honeycomb matrix; 51. Imitation honeycomb hole A; 52. Imitation honeycomb hole B; 53. Imitation honeycomb hole C; 54. Imitation honeycomb hole D; 55. Imitation honeycomb hole E; 56. Imitation honeycomb hole F; 6. Liquid storage plate; 7. Liquid storage plate hole; 8. Flow limiting baffle; 9. Air flow slot; 91. First slot; 92. Second slot; 10. Matrix gap; 11. First air-permeable arc-shaped protrusion; 12. Second air-permeable arc-shaped protrusion; 13. Anti-pollution protrusion ring. DETAILED DESCRIPTION

[0033] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that, in the absence of conflict, the embodiments of the present application and the features therein can be combined with each other.

[0034] In the present invention, the term "plurality" refers to two or more, unless otherwise expressly defined. The term "and / or" used herein includes any and all combinations of one or more of the relevant listed items. Terms such as "installed", "connected", "connected", and "fixed" should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; "connected" can be a direct connection or an indirect connection through an intermediate medium. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0035] It should be noted that when an element is referred to as being "assembled to," "mounted to," "fixed to," or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation methods.

[0036] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0037] like Figures 1-10 As shown, the present invention provides an evaporation compensation device, including a liquid storage plate cover 1 integrally formed by a middle plate 101, an upper outer wall 102 and a lower outer wall 103, the surface of the middle plate 101 is connected with a plurality of circular raised rings 2 arranged on the inner side of the lower outer wall 103 and a plurality of protrusions 3 arranged on the inner side of the upper outer wall 102, the plurality of protrusions 3 are designed in a ring-shaped stepped manner from the outer periphery to the inner circle, and a plurality of cavities 4 are opened on one side of the circular raised ring 2 on the surface of the middle plate 101, the plurality of cavities 4 are respectively arranged concentrically with the plurality of circular raised rings 2 and the protrusions 3 and correspond to each other, and an imitation honeycomb matrix 5 is formed on the liquid storage plate cover 1 by the cooperation of the circular raised rings 2, the protrusions 3 and the cavities 4, the imitation honeycomb matrix 5 corresponds to the plurality of liquid storage plate holes 7 on the liquid storage plate 6, the four corners of the imitation honeycomb matrix 5 are respectively provided with flow limiting baffles 8 connected to the middle plate 101, and an air flow slot 9 for air flow is opened on the upper outer wall 102.

[0038] As a further example, the simulated honeycomb matrix 5 includes simulated honeycomb holes A51, simulated honeycomb holes B52, simulated honeycomb holes C53, simulated honeycomb holes D54, simulated honeycomb holes E55 and simulated honeycomb holes F56. There are 2n simulated honeycomb holes A51, simulated honeycomb holes B52, simulated honeycomb holes C53, simulated honeycomb holes D54, simulated honeycomb holes E55 and simulated honeycomb holes F56, respectively, where n≥2. Several simulated honeycomb holes A51 are located at the four corners of the outer edge of the simulated honeycomb matrix 5, and several simulated honeycomb holes Hole F56 is located at the center of the imitation honeycomb matrix 5, several imitation honeycomb holes E55 are divided into two groups and symmetrically arranged with the imitation honeycomb hole F56 as the symmetry axis, several imitation honeycomb holes D54 form a rectangular frame shape and are distributed around the imitation honeycomb holes E55 and the imitation honeycomb holes F56, several imitation honeycomb holes C53 form a rectangular frame shape and are distributed around the imitation honeycomb holes D54, several imitation honeycomb holes B52 are distributed around the imitation honeycomb holes C53, and form a rectangular frame shape with several imitation honeycomb holes A51.

[0039] Combine Figure 3 、 Figure 8 and Figure 9 As shown, as a further improvement, since 96 wells are the most commonly used in the multi-well liquid storage plate 6, this solution is mainly implemented with a 96-well liquid storage plate 6 and a matching evaporation compensation plate cover. Figure 9 The liquid storage plate holes 7 of the liquid storage plate 6 shown in the figure correspond to each simulated honeycomb in the simulated honeycomb matrix 5; thus, this solution forms a simulated honeycomb-shaped liquid storage plate cover 1 through the multiple special structures of the simulated honeycomb matrix 5, the air flow slots 9 and the circular raised ring 2, and the liquid storage plate cover 1 is combined with the liquid storage plate 6 to form an integral liquid storage device with an evaporation compensation function; ensuring that the liquid in the liquid storage plate 6 is in a relatively sealed environment with the liquid storage plate cover 1, the evaporation amount of the liquid in each liquid storage plate hole 7 is relatively consistent, thereby achieving high repeatability of the experimental reaction conditions of each liquid storage plate hole 7.

[0040] More specifically, combined Figure 3 As shown, the simulated honeycomb matrix 5 is distributed in an 8×12 matrix; There are four simulated honeycomb holes A51, which are located at the four corners of the outer edge of the simulated honeycomb matrix 5; There are four simulated honeycomb holes F56, which are located at the center of the simulated honeycomb matrix 5; There are eight imitation honeycomb holes E55, which are divided into two groups and symmetrically arranged with the imitation honeycomb hole F56 as the symmetry axis; There are twelve simulated honeycomb holes D54, which are arranged in a rectangular frame shape around the simulated honeycomb holes E55 and F56. There are 28 simulated honeycomb holes C53, which are distributed around the simulated honeycomb hole D54 in a rectangular frame shape. There are thirty-two simulated honeycomb holes B52 distributed around the periphery of the simulated honeycomb hole C53 , and the multiple simulated honeycomb holes B52 and the four simulated honeycomb holes A51 form a rectangular frame.

[0041] Combine Figure 8 and Figure 9 As shown, in the prior art, during the process of placing the culture medium in the liquid storage plate 6, the liquid storage plate holes 7 will mostly generate heat energy due to chemical reactions. However, due to the sealing of the liquid storage plate cover 1, the heat energy diffusion of each liquid storage plate hole 7 is inconsistent, resulting in the air between the liquid storage plate 6 and the liquid storage plate cover 1 to generate air flow from the inside to the outside, which also indirectly leads to the overall performance of the liquid evaporation amount of each liquid storage plate hole 7 at the same time and under the same environment. Figure 9As shown, a series of evaporation gradients are formed, with the order being evaporation in the red area > evaporation in the orange area > evaporation in the yellow area > evaporation in the green area > evaporation in the white area > evaporation in the blue area. Furthermore, to ensure that the evaporation in each area of ​​the liquid reservoir hole 7 of the liquid reservoir plate 6 is relatively consistent, the present invention, based on Dalton's law of evaporation (W=C(Ee) / p), can employ various methods, including temperature compensation, evaporation compensation, and reducing the air flow rate at the liquid surface. It should be noted that in the Dalton's law of evaporation proposed in the present invention, W is the evaporation rate at the water surface, (E-e) is the saturation difference of the air, E is the saturated water vapor pressure at the water surface gas temperature, e is the actual water vapor pressure of the air at the water surface, P is the air pressure, and C is a proportional coefficient related to wind speed.

[0042] It is worth noting that in order to ensure that the evaporation amount in each area of ​​the liquid storage plate hole 7 is relatively consistent, the various methods adopted by the present invention, such as temperature compensation, evaporation amount compensation, and reduction of liquid surface air flow rate, are complementary to each other.

[0043] As a further step, combined Figure 1 、 Figure 6 and Figure 7 As shown, the height of the convex column 3 corresponding to the imitation honeycomb hole B52 in the imitation honeycomb matrix 5 is less than the height of the convex column 3 corresponding to the imitation honeycomb hole C53 in the imitation honeycomb matrix 5, the height of the convex column 3 corresponding to the imitation honeycomb hole C53 in the imitation honeycomb matrix 5 is less than the height of the convex column 3 corresponding to the imitation honeycomb hole D54 in the imitation honeycomb matrix 5, the height of the convex column 3 corresponding to the imitation honeycomb hole D54 in the imitation honeycomb matrix 5 is less than the height of the convex column 3 corresponding to the imitation honeycomb hole E55 in the imitation honeycomb matrix 5, and the height of the convex column 3 corresponding to the imitation honeycomb hole E55 in the imitation honeycomb matrix 5 is less than the height of the convex column 3 corresponding to the imitation honeycomb hole F56 in the imitation honeycomb matrix 5.

[0044] Combine Figures 1-10 As shown, as an implementation method, that is, a temperature compensation method, the ambient temperature of the central plate hole is lowered and the ambient temperature of the edge plate hole is increased. The method is specifically as follows: the height of the simulated honeycomb hole A51 is designed to be less than the height of the simulated honeycomb hole B52 < the height of the simulated honeycomb hole C53 < the height of the simulated honeycomb hole D54 < the height of the simulated honeycomb hole E55 < the height of the simulated honeycomb hole F56, ensuring that the simulated honeycomb matrix 5 structure on the liquid storage plate cover 1 can form a circle of staggered and annularly symmetrical cylindrical structures according to the number of liquid storage plate holes 7, and then the ambient temperature of the liquid storage plate holes 7 is adjusted by the height of the convex columns 3 of the simulated honeycomb matrix 5 of the liquid storage plate cover 1 to achieve a gradient insulation function, thereby realizing temperature compensation adjustment.

[0045] Combine Figure 2 and Figure 6 As shown, as a further feature, the bosses 3 are distributed in a rectangular array, and a matrix gap 10 is provided between every two adjacent bosses 3 .

[0046] By utilizing the design of the matrix gap 10 between every two protrusions 3 in the honeycomb matrix 5 of the liquid storage plate cover 1, the honeycomb structures on the liquid storage plate cover 1 can be ensured to be independent of each other, ensuring smooth air flow when the liquid storage plates 6 are stacked, which is beneficial to the average diffusion and conduction of heat from the liquid storage plates 6, ensuring that the heat diffusion at the center and edge of the liquid storage plates 6 is relatively uniform, thereby reducing the edge evaporation effect.

[0047] Combine Figure 1 As shown, the airflow slots 9 include a first slot 91 and a second slot 92 perpendicular to the first slot 91. Two first slots 91 and two second slots 92 are provided, and the two first slots 91 are arranged parallel to each other and are correspondingly provided on two sides of the upper outer wall 102. The two second slots 92 are arranged parallel to each other and are correspondingly provided on the other two sides of the upper outer wall 102. The first slots 91 and the second slots 92 are each arc-shaped, with the centers of the two first slots 91 and the centers of the corresponding sides of the upper outer wall 102 respectively on the same vertical line. The centers of the two second slots 92 and the centers of the corresponding sides of the upper outer wall 102 respectively on the same vertical line.

[0048] By utilizing the design of the first notch 91 and the second notch 92 on the outer wall 102 of the liquid storage plate cover 1, the air flow rate from the upper edge to the center of the liquid storage plate cover 1 can be gradually increased, thereby ensuring that temperature compensation is achieved when the liquid storage plates 6 are stacked to reduce the edge evaporation effect.

[0049] In summary, specifically, in conventional experimental processes, due to the large number of specimens, it is often necessary to stack the processed and covered liquid storage plates 6, and the stacked liquid storage plates 6 also have temperature differences, that is, the center temperature is higher; therefore, in order to further perform temperature compensation, two mutually corresponding first notches 91 and two mutually corresponding second notches 92 are designed along the outer wall 102 of the liquid storage plate cover 1, so as to gradually increase the air flow rate at the center of the upper part of the liquid storage plate cover 1 and gradually reduce the air flow rate at the four corners of the liquid storage plate 6, thereby achieving a temperature compensation effect; and, by designing the matrix gap 10 between every two convex columns 3 in the simulated honeycomb matrix 5, it can be ensured that the honeycomb structures above the liquid storage plate cover 1 are independent of each other, ensuring smooth air flow, which is conducive to the average diffusion and conduction of heat.

[0050] Combine Figure 3 、 Figure 4 and Figure 5 As shown, as a further example, the simulated honeycomb holes A51 located at the four corners of the outer edge of the simulated honeycomb matrix 5 are formed by circular raised rings 2, and the four corners of the simulated honeycomb matrix 5 are respectively provided with flow limiting baffles 8 connected to the middle plate 101, and the flow limiting baffles 8 at the four corners of the simulated honeycomb matrix 5 are respectively fixedly connected to the outer walls of the corresponding circular raised rings 2.

[0051] As an implementation method, by adding four flow-limiting baffles 8, the air flow rate and flow of the liquid storage plate holes 7 corresponding to the imitation honeycomb holes A51 at the four corners of the liquid storage plate cover 1 can be effectively reduced, ensuring that the air flow above the liquid storage plate holes 7 corresponding to the four corners of the liquid storage plate cover 1 is consistent with other liquid storage plate holes 7, effectively reducing the evaporation amount of the liquid storage plate holes 7 corresponding to the imitation honeycomb holes A51; at the same time, with the help of the design of four flow-limiting baffles 8, the overall height of the inner cylinder of the cylindrical structure of the imitation honeycomb matrix 5 can be effectively reduced, thereby achieving the purpose of saving production raw materials.

[0052] Furthermore, the inner side wall of the lower outer wall 103 is provided with three first breathable arc-shaped protrusions 11 connected to the middle plate 101 and one second breathable arc-shaped protrusion 12 connected to the middle plate 101, and the three first breathable arc-shaped protrusions 11 and one second breathable arc-shaped protrusion 12 are respectively provided at the four corners of the middle plate 101, and are respectively connected to the inner side wall of the lower outer wall 103.

[0053] As a further step, combined Figure 1 、 Figure 3 、 Figure 6 and Figure 7 As shown, the circular raised rings 2 are distributed in a rectangular array, and the volume of the cavity 4 of the simulated honeycomb hole A51 in the simulated honeycomb matrix 5 is smaller than the volume of the cavity 4 of the simulated honeycomb hole B52 in the simulated honeycomb matrix 5, the volume of the cavity 4 of the simulated honeycomb hole B52 in the simulated honeycomb matrix 5 is smaller than the volume of the cavity 4 of the simulated honeycomb hole C53 in the simulated honeycomb matrix 5, the volume of the cavity 4 of the simulated honeycomb hole C53 in the simulated honeycomb matrix 5 is smaller than the volume of the cavity 4 of the simulated honeycomb hole D54 in the simulated honeycomb matrix 5, the volume of the cavity 4 of the simulated honeycomb hole D54 in the simulated honeycomb matrix 5 is smaller than the volume of the cavity 4 of the simulated honeycomb hole E55 in the simulated honeycomb matrix 5, and the volume of the cavity 4 of the simulated honeycomb hole E55 in the simulated honeycomb matrix 5 is smaller than the volume of the cavity 4 of the simulated honeycomb hole F56 in the simulated honeycomb matrix 5.

[0054] Combine Figures 1-10 As shown in FIG. 1 , as an implementation method, i.e., an evaporation compensation method, a method is used in which the volume of the central honeycomb hole is larger than the volume of the edge honeycomb hole. Specifically, the method is as follows: the volume of the simulated honeycomb hole A51 < the volume of the simulated honeycomb hole B52 < the volume of the simulated honeycomb hole C53 < the volume of the simulated honeycomb hole D54 < the volume of the simulated honeycomb hole E55 < the volume of the simulated honeycomb hole F56. Therefore, by adopting this volume design, the present invention can compensate and adjust the evaporation percentage in each liquid storage plate hole 7 in the liquid storage plate 6, ensuring that Figure 9 As shown, the evaporation percentage of the red area < the evaporation percentage of the orange area < the evaporation percentage of the yellow area < the evaporation percentage of the green area < the evaporation percentage of the white area < the evaporation percentage of the blue area, ensuring that the present invention can achieve compensation adjustment for the evaporation amount in each liquid storage plate hole 7.

[0055] Combine Figure 4 and Figure 8 As shown, as a further example, an evaporation compensation device also includes a plurality of anti-pollution raised rings 13 connected to the surface of the liquid storage plate 6, and the plurality of anti-pollution raised rings 13 correspond one-to-one to the plurality of liquid storage plate holes 7, and the anti-pollution raised rings 13 are used to interference fit with the circular raised ring 2.

[0056] Specifically, as an implementation method, that is, a method for reducing the air flow rate at the liquid surface, the method is specifically: the mutual interference fit between the circular raised ring 2 and the anti-pollution raised ring 13 can be used to strengthen the sealing of each liquid storage plate hole 7, so as to minimize the air flow rate, thereby reducing the evaporation amount of liquid in each liquid storage plate hole 7.

[0057] The working principle and usage process of the present invention: The present invention forms a circle of cylindrical structures with staggered heights and annular symmetry according to the number of liquid storage plate holes 7 through the honeycomb matrix 5 structure on the liquid storage plate cover 1, so that the liquid storage plate cover 1 can achieve a gradient insulation function, thereby realizing temperature compensation for the liquid storage plate holes 7.

[0058] The present invention achieves compensation adjustment of the evaporation percentage in each liquid storage plate hole 7 in the liquid storage plate 6 by making the volume of the central honeycomb hole of the simulated honeycomb matrix 5 on the liquid storage plate cover 1 larger than the volume of the edge honeycomb holes, thereby realizing the evaporation compensation function of the liquid storage plate hole 7.

[0059] The present invention greatly reduces the air flow rate above the liquid storage plate hole 7 by the mutual interference fit between the circular raised ring 2 and the anti-pollution raised ring 13 compared with the traditional liquid storage plate 6 and liquid storage plate cover 1 design, thereby reducing the overall evaporation amount of the liquid storage plate hole 7, thereby narrowing the difference in evaporation amount of each liquid storage plate hole 7 and reducing the edge evaporation effect.

[0060] The present invention can effectively reduce the air flow of the liquid storage plate holes 7 corresponding to the imitation honeycomb holes A51 at the four corners of the liquid storage plate cover 1 by adding four flow limiting baffles 8, ensuring that the air flow above the liquid storage plate holes 7 corresponding to the four corners of the liquid storage plate cover 1 is consistent with that of other liquid storage plate holes 7, effectively reducing the evaporation amount of the liquid storage plate holes 7 corresponding to the imitation honeycomb holes A51.

[0061] When the liquid storage plates 6 are stacked, the present invention can gradually increase the air flow rate at the center of the upper part of the liquid storage plate cover 1 and gradually reduce the air flow rate at the four corners of the liquid storage plate 6 through the first notch 91 and the second notch 92 designed on the upper outer wall 102, thereby achieving temperature compensation; and, through the design of the matrix gap 10 between every two protrusions 3 in the honeycomb matrix 5, it can be ensured that the honeycomb structures above the liquid storage plate cover 1 are independent of each other, ensuring smooth air flow, which is conducive to the average diffusion and conduction of heat.

[0062] In summary, through the collaborative design of the above five structural principles, the statistical percentage of evaporation amount of each plate hole of the 96-well evaporation compensation device liquid storage plate 6 is as follows: Figure 10 As shown, the "edge evaporation effect" problem of the 96-well plate can be effectively solved, thereby improving the uniformity of the experimental conditions of each reservoir plate well 7 and ensuring the repeatability of the experimental results.

[0063] It should be noted that the liquid storage plate cover 1 proposed in the present invention adopts an imitation honeycomb matrix 5 design to achieve evaporation compensation for the liquid storage plate holes 7. It has a simple structure and is convenient for mold production. It is compatible with traditional liquid storage plates 6 and does not change the experimental steps. The operation is simple and convenient.

[0064] Throughout this specification, terms such as "one embodiment," "some embodiments," and "specific embodiments" mean that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0065] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and alterations may be made to the embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the claims and their equivalents.

Claims

1. An evaporation compensation device, characterized in that: The invention comprises a liquid storage plate cover (1) integrally formed by a middle plate (101), an upper outer wall (102) and a lower outer wall (103); the surface of the middle plate (101) is connected with a plurality of circular raised rings (2) provided on the inner side of the lower outer wall (103) and a plurality of convex columns (3) provided on the inner side of the upper outer wall (102); the plurality of convex columns (3) are designed in an annular stepped manner from the outer periphery to the inner circle; and the surface of the middle plate (101) is provided with a plurality of cavities (4) on one side of the circular raised ring (2); the plurality of cavities ( 4) are respectively arranged concentrically with the plurality of circular raised rings (2) and the raised columns (3) and correspond to each other, and an imitation honeycomb matrix (5) is formed on the liquid storage plate cover (1) through the cooperation of the circular raised rings (2), the raised columns (3) and the cavity (4), and the imitation honeycomb matrix (5) corresponds to the plurality of liquid storage plate holes (7) on the liquid storage plate (6), and flow limiting baffles (8) connected to the middle plate (101) are respectively arranged at the four corners of the imitation honeycomb matrix (5), and an air flow slot (9) for air flow is opened on the upper outer wall (102).

2. The evaporation compensation device according to claim 1, characterized in that: The simulated honeycomb matrix (5) includes simulated honeycomb holes A (51), simulated honeycomb holes B (52), simulated honeycomb holes C (53), simulated honeycomb holes D (54), simulated honeycomb holes E (55) and simulated honeycomb holes F (56), wherein the simulated honeycomb holes A (51), simulated honeycomb holes B (52), simulated honeycomb holes C (53), simulated honeycomb holes D (54), simulated honeycomb holes E (55) and simulated honeycomb holes F (56) are respectively provided in a number of 2n, wherein n≥2, and a number of the simulated honeycomb holes A (51) are located at the four corners of the outer edge of the simulated honeycomb matrix (5), and a number of the simulated honeycomb holes F (56) are located at the outer corners of the simulated honeycomb matrix (5). At the center of the simulated honeycomb matrix (5), a plurality of the simulated honeycomb holes E (55) are divided into two groups and are symmetrically arranged with the simulated honeycomb hole F (56) as the symmetry axis. A plurality of the simulated honeycomb holes D (54) form a rectangular frame shape and are distributed around the simulated honeycomb holes E (55) and the simulated honeycomb holes F (56). A plurality of the simulated honeycomb holes C (53) form a rectangular frame shape and are distributed around the simulated honeycomb holes D (54). A plurality of the simulated honeycomb holes B (52) are distributed around the simulated honeycomb holes C (53) and form a rectangular frame shape with a plurality of the simulated honeycomb holes A (51).

3. The evaporation compensation device according to claim 2, characterized in that: The height of the convex column (3) corresponding to the simulated honeycomb hole B (52) in the simulated honeycomb matrix (5) is less than the height of the convex column (3) corresponding to the simulated honeycomb hole C (53) in the simulated honeycomb matrix (5); the height of the convex column (3) corresponding to the simulated honeycomb hole C (53) in the simulated honeycomb matrix (5) is less than the height of the convex column (3) corresponding to the simulated honeycomb hole D (54) in the simulated honeycomb matrix (5); the height of the convex column (3) corresponding to the simulated honeycomb hole D (54) in the simulated honeycomb matrix (5) is less than the height of the convex column (3) corresponding to the simulated honeycomb hole E (55) in the simulated honeycomb matrix (5); the height of the convex column (3) corresponding to the simulated honeycomb hole E (55) in the simulated honeycomb matrix (5) is less than the height of the convex column (3) corresponding to the simulated honeycomb hole F (56) in the simulated honeycomb matrix (5).

4. The evaporation compensation device according to claim 3, characterized in that: The convex columns (3) are distributed in a rectangular array, and a matrix gap (10) is provided between every two adjacent convex columns (3).

5. The evaporation compensation device according to claim 2, characterized in that: The simulated honeycomb holes A (51) located at the four corners of the outer edge of the simulated honeycomb matrix (5) are formed by circular raised rings (2), and the flow limiting baffles (8) at the four corners of the simulated honeycomb matrix (5) are respectively fixedly connected to the outer walls of the corresponding circular raised rings (2).

6. The evaporation compensation device according to claim 5, characterized in that: The circular raised rings (2) are distributed in a rectangular array, and the volume of the cavity (4) of the simulated honeycomb hole A (51) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole B (52) in the simulated honeycomb matrix (5), the volume of the cavity (4) of the simulated honeycomb hole B (52) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole C (53) in the simulated honeycomb matrix (5), and the volume of the cavity (4) of the simulated honeycomb hole C (53) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole B (52) in the simulated honeycomb matrix (5). The volume of the cavity (4) of the simulated honeycomb hole D (54) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole D (54) in the simulated honeycomb matrix (5), the volume of the cavity (4) of the simulated honeycomb hole D (54) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole E (55) in the simulated honeycomb matrix (5), and the volume of the cavity (4) of the simulated honeycomb hole E (55) in the simulated honeycomb matrix (5) is smaller than the volume of the cavity (4) of the simulated honeycomb hole F (56) in the simulated honeycomb matrix (5).

7. The evaporation compensation device according to claim 1, characterized in that: The air flow slot (9) comprises a first slot (91) and a second slot (92) perpendicular to the first slot (91), wherein two first slots (91) and two second slots (92) are respectively provided, and the two first slots (91) are provided in parallel with each other and are correspondingly provided on two side surfaces of the upper outer wall (102), and the two second slots (92) are provided in parallel with each other and are correspondingly provided on the other two side surfaces of the upper outer wall (102).

8. The evaporation compensation device according to claim 7, characterized in that: The first notch (91) and the second notch (92) are respectively in an arc shape, the centers of the two first notches (91) are respectively on the same vertical line with the center points of the corresponding side surfaces of the upper outer wall (102), and the centers of the two second notches (92) are respectively on the same vertical line with the center points of the corresponding side surfaces of the upper outer wall (102).

9. The evaporation compensation device according to claim 1, characterized in that: The inner side wall of the lower outer wall (103) is provided with three first breathable arc-shaped protrusions (11) connected to the middle plate (101) and one second breathable arc-shaped protrusion (12) connected to the middle plate (101), and the three first breathable arc-shaped protrusions (11) and one second breathable arc-shaped protrusion (12) are respectively provided at the four corners of the middle plate (101) and are respectively connected to the inner side wall of the lower outer wall (103).

10. The evaporation compensation device according to claim 1, characterized in that: It also includes a plurality of anti-pollution raised rings (13) connected to the surface of the liquid storage plate (6), and the plurality of anti-pollution raised rings (13) respectively correspond to the plurality of liquid storage plate holes (7), and the anti-pollution raised rings (13) are used to have an interference fit with the circular raised ring (2).

Images