NMN-containing female gel composition and preparation method thereof

Abstract

The invention relates to the technical field of medical supplies, and particularly provides a female gel composition containing NMN. The composition is prepared from the following raw materials in percentage by weight: 0.8 to 1.5 percent of carbomer (940), 0.5 to 1.5 percent of isomaltooligosacharide, 2 to 4 percent of glycerol, 0.005 to 0.02 percent of beta-nicotinamide mononucleotide (NMN), 0.001 to 0.01 percent of ethylene diamine tetraacetic acid, 0.01 to 0.03 percent of ethylparaben, 0.5 to 1 percent of propylene glycol, 0.5 to 2 percent of triethanolamine and the balance of purified water. The composition is good in moisturizing performance, safe and free of irritation.

Description

Technical Field

[0001] The present invention relates to the technical field of medical supplies, and in particular to a gel composition for females containing NMN and a preparation method thereof. Background Art

[0002] The care and anti-aging of women's private parts have always been important concerns in the field of women's health. With age and the influence of the external environment, the firmness and elasticity of women's private parts may gradually decrease, leading to dryness, discomfort and other problems, seriously affecting women's quality of life and self-confidence. Traditional women's care products mostly focus on cleansing or a single moisturizing function. However, with the increasing diversification and personalization of women's demand for private care products, the market urgently needs a product that can provide comprehensive care, firming and anti-aging.

[0003] In recent years, β-nicotinamide mononucleotide (NMN) has gradually attracted attention as an emerging anti-aging ingredient. NMN can be converted into NAD+ in the body, which is a key coenzyme involved in cell metabolism and repair. Studies have shown that NMN has significant antioxidant, anti-inflammatory, and cell regeneration and repair functions, which makes it have broad application prospects in the anti-aging field. Women's private parts have relatively high requirements for product safety, such as requiring products to be mild and non-irritating and not affect the skin's microenvironment. Currently, there is no targeted research on these aspects of NMN-related products. At the same time, continuous moisturizing properties can maintain its good care performance. Summary of the Invention

[0004] The object of the present invention is to provide a gel composition for women containing NMN and a preparation method to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: A gel composition for women containing NMN, wherein the composition is prepared from the following raw materials in percentage by weight: 0.8-1.5% of carbomer (940), 0.5-1.5% of isomaltooligosaccharide, 2-4% of glycerol, 0.005-0.02% of β-nicotinamide mononucleotide (NMN), 0.001-0.01% of disodium edetate, 0.01-0.03% of ethylparaben, 0.5-1% of propylene glycol, 0.5-2% of triethanolamine, and the balance being purified water.

[0006] Preferably, the composition is prepared from the following raw materials in weight percentage: carbomer (940) 1.2%, isomaltooligosaccharide 0.5%, glycerol 2%, β-nicotinamide mononucleotide (NMN) 0.01%, disodium edetate 0.003%, ethylparaben 0.01%, propylene glycol 0.5%, triethanolamine 0.5%, and the balance is purified water.

[0007] Preferably, the composition is prepared from the following raw materials in weight percentage: carbomer (940) 1.0%, isomaltooligosaccharide 1%, glycerol 3%, β-nicotinamide mononucleotide (NMN) 0.005%, disodium edetate 0.005%, ethylparaben 0.02%, propylene glycol 1.0%, triethanolamine 0.9%, and the balance is purified water.

[0008] Preferably, the composition is prepared from the following raw materials in weight percentage: carbomer (940) 1.5%, isomaltooligosaccharide 1.5%, glycerol 2.5%, β-nicotinamide mononucleotide (NMN) 0.02%, disodium edetate 0.01%, ethylparaben 0.03%, propylene glycol 1%, triethanolamine 2.0%, and the balance is purified water.

[0009] Preferably, the purified water is medical purified water with a maximum microbial limit of 100 cfu / ml.

[0010] Preferably, the pH of the composition is 3.8 to 6.5.

[0011] A method for preparing the above-mentioned gel composition for women containing NMN comprises the following steps: Weigh a certain amount of material and set aside; Add carbomer (940) to a container filled with purified water and stir. After it is completely dissolved, add β-nicotinamide mononucleotide (NMN), oligoisomaltodextrose, glycerol, propylene glycol, disodium edetate, and ethyl hydroxybenzoate to the container, mix and stir for 4-5 hours, add triethanolamine to adjust the pH value to 3.8-6.5, and let it stand to form a gel; After the gel is sampled and tested to be qualified, it is filled by the filling machine and packaged as a finished product.

[0012] Technical effects and advantages of the present invention: NMN in the gel composition of the present invention is an important anti-aging ingredient that can be converted into NAD+ in the body, promoting cell metabolism and repair, and slowing down the aging process of private parts. At the same time, the antioxidant function of NMN helps to scavenge free radicals, reduce damage to the skin by the external environment, and delay aging. Therefore, it has good moisturizing properties when combined with other components and can be used for firming and anti-aging of women's private parts.

[0013] The composition of the present invention has carried out targeted research and selection on the main components of the gel according to the selected active ingredients, wherein carbomer is used as a gel matrix. Carbomer forms a three-dimensional network structure through hydrogen bonding and electrostatic interaction, giving the gel stability and thixotropy, and further adding glycerol and oligoisomaltose. Glycerol can regulate the swelling behavior of the gel, and the hydroxyl group of oligoisomaltose forms a weak interaction with the carboxyl group of carbomer to enhance network stability. Glycerol combines with the hydrophobic group of carbomer to form a two-phase lubrication, thereby improving the spreadability and adhesion of the gel. Oligoisomaltose and glycerol can further cooperate with the moisturizing network. The comprehensive synergistic effect of these gel components significantly improves the moisturizing performance of the NMN gel product containing this application, can effectively maintain the moisture balance of private parts, reduce dryness and discomfort, provide a mild soothing effect, have low irritation, and enhance the comfort after use; The composition has been carefully designed and uses safe and mild ingredients, making it suitable for use on women's private parts. The carbomer and triethanolamine in the formula work synergistically to ensure that the pH value of the product is controlled between 3.8 and 6.5, which is close to the natural acid-base environment of women's private parts and reduces the risk of skin irritation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the preparation process of the gel composition for women containing NMN of the present invention.

[0015] Figure 2 This is a schematic diagram of the intradermal induction injection area in an embodiment of the present invention. DETAILED DESCRIPTION

[0016] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention. Example 1

[0017] The gel composition is prepared from the following raw materials in percentage by weight: 1.2% carbomer (940), 0.5% isomaltooligosaccharide, 2% glycerol, 0.01% β-nicotinamide mononucleotide (NMN), 0.003% disodium edetate, 0.01% ethylparaben, 0.5% propylene glycol, 0.5% triethanolamine, and the balance is purified water. The pH value is 3.8-6.5.

[0018] The preparation method thereof comprises the following steps: Weigh a certain amount of material and set aside; Add carbomer (940) to a container filled with purified water and stir. After it is completely dissolved, add β-nicotinamide mononucleotide (NMN), oligoisomaltodextrose, glycerol, propylene glycol, disodium edetate, and ethyl hydroxybenzoate to the container, mix and stir for 4-5 hours, add triethanolamine to adjust the pH value to 3.8-6.5, and let it stand to form a gel; After the gel is sampled and tested to be qualified, it is filled by the filling machine and packaged as a finished product. Example 2

[0019] The gel composition is prepared from the following raw materials in weight percentage: carbomer (940) 1.0%, isomaltooligosaccharide 1%, glycerol 3%, β-nicotinamide mononucleotide (NMN) 0.005%, disodium edetate 0.005%, ethylparaben 0.02%, propylene glycol 1.0%, triethanolamine 0.9%, and the balance is purified water. The pH value is 3.8-6.5.

[0020] The preparation method is the same as that of Example 1. Example 3

[0021] The gel composition is prepared from the following raw materials in percentage by weight: 1.5% carbomer (940), 1.5% isomaltooligosaccharide, 2.5% glycerol, 0.02% β-nicotinamide mononucleotide (NMN), 0.01% disodium edetate, 0.03% ethylparaben, 1% propylene glycol, 2.0% triethanolamine, and the balance is purified water. The pH is 3.8-6.5.

[0022] The preparation method is the same as that of Example 1.

[0023] Comparative Example 1 The gel composition is prepared from the following raw materials in percentage by weight: 1.0% carboxypropyl cellulose, 1% isomaltooligosaccharide, 3% glycerol, 0.005% β-nicotinamide mononucleotide (NMN), 0.005% disodium edetate, 0.02% ethylparaben, 1.0% propylene glycol, 0.9% triethanolamine, and the balance purified water. The pH is 3.8-6.5. The preparation method is similar to that in Example 1.

[0024] Comparative Example 2 The gel composition is prepared from the following raw materials in percentage by weight: 1.0% xanthan gum, 1% isomaltooligosaccharide, 3% glycerol, 0.005% β-nicotinamide mononucleotide (NMN), 0.005% disodium EDTA, 0.02% ethylparaben, 1.0% propylene glycol, 0.9% triethanolamine, and the balance purified water. The pH is 3.8-6.5. The preparation method is similar to that in Example 1.

[0025] Comparative Example 3 The gel composition is prepared from the following raw materials in percentage by weight: 1.0% carbomer (940), 4% isomaltooligosaccharide, 0.005% β-nicotinamide mononucleotide (NMN), 0.005% disodium edetate, 0.02% ethylparaben, 1.0% propylene glycol, 0.9% triethanolamine, and the balance is purified water. The pH is 3.8-6.5. The preparation method is similar to that in Example 1.

[0026] Comparative Example 4 The gel composition is prepared from the following raw materials in percentage by weight: 1.0% carbomer (940), 4% glycerol, 0.005% β-nicotinamide mononucleotide (NMN), 0.005% disodium edetate, 0.02% ethyl paraben, 1.0% propylene glycol, 0.9% triethanolamine, and the balance is purified water. The pH is 3.8-6.5. The preparation method is similar to that in Example 1.

[0027] Comparative Example 5 The gel composition is prepared from the following raw materials in percentage by weight: 1.0% carbomer (940), 0.005% β-nicotinamide mononucleotide (NMN), 0.005% disodium edetate, 0.02% ethylparaben, 5.0% propylene glycol, 0.9% triethanolamine, and the balance is purified water. The pH is 3.8-6.5, and the preparation method is similar to that of Example 1. The above three groups of examples of the present application can all produce female intimate firming and anti-aging gel compositions containing NMN. Among them, the gel composition prepared in the second group of examples has the best effect, which is verified by the following experiments in the study: Test Example 1: Coating and Moisturizing Performance Test: Coating Performance Determination: Uniform coating is a key indicator of good gel coating. During the coating process, observe whether the gel is evenly distributed on the substrate and whether there are any uneven thickness or patchiness. Adhesion is also preliminarily determined based on the ease of coating. Low adhesion means the gel flows easily after coating, while high adhesion may cause an unpleasant sensation on the skin. In this application, the gel is evenly applied to a clean glass slide, its uniformity is observed, and adhesion is determined experimentally. Moisturizing Performance Determination: 2g of the prepared gel composition is weighed and placed in a desiccator containing an adsorbent such as silica gel. The sample mass is measured after 36 and 72 hours. Moisturizing Percentage = Sample mass after the specified time of stagnation / Sample mass before stagnation × 100%.

[0028] From the coating performance point of view, comparative examples 1 and 2 are not very ideal, either the adhesion is too large or there are bubbles in the coating, while the coating performance of other examples is good.

[0029] At the same time, it can be seen from the above test results that the gel composed of carbomer, oligomeric isomaltosaccharides, and glycerol in this application produces a significant synergistic effect. Due to the synergy of these three components, the moisturizing performance of the gel is significantly improved. When oligomeric isomaltosaccharides and glycerol are used in combination, the moisturizing performance is the best. Reducing one of oligomeric isomaltosaccharides and glycerol will directly affect the moisturizing performance of the gel. In addition, the moisturizing is closely related to the type of gel matrix. When the same component dosage is used, the gel matrix is ​​changed from carbomer to other gel matrix components such as xanthan gum and hydroxypropyl cellulose. Not only does the moisturizing performance decrease, but its coating performance is also poor. The embodiment has a significant effect difference compared with the comparative example.

[0030] As we all know, for gel products with specific active ingredients, their corresponding compositions will be different, and their applicable objects and parts also affect the screening of prescriptions. The above research results of this application are the creative research results of this application, which are the technical innovations of continuously trying and combining the theories and performances of the components in the experiments with the active ingredients of this application. The carbomer selected in this application forms a three-dimensional network structure through hydrogen bonds and electrostatic effects, giving the gel stability and thixotropy. Glycerol and oligomaltitol are further added. Glycerol can regulate the swelling behavior of the gel. The hydroxyl groups of oligomaltitol form weak interactions with the carboxyl groups of carbomer, enhancing the network stability. Glycerol combines with the hydrophobic groups of carbomer to form a two-phase lubrication, improving the spreadability and adhesion of the gel. Oligomaltitol and glycerol can further synergize the moisturizing network. The above specific experimental results further verify the interaction and mutual synergy of these components.

[0031] Test Example 2. Composition - Skin Sensitization Test (GPMT Method) 2.1 Purpose The maximum dose method test (GPMT) was used to evaluate the potential skin sensitization of the sample extracts in guinea pigs.

[0032] 2.2 Test information This application: The gel prepared by Example 2 Polarity: 0.9% Sodium Chloride Injection (SC).

[0033] Non-polar: corn oil (CO).

[0034] Positive control: 2,4-dinitrochlorobenzene (DNCB).

[0035] Experimental Animal and Husbandry Information: Hartley guinea pigs. Weight at the start of the experiment: 300-500 g. Stocking density: 5 per cage. Animal identification: Body marking with a dye pen and cage tags attached to the cages. Feed: Guinea pig maintenance diet, 40 g / day / guinea pig. Drinking water: Purified water, available ad libitum in separate drinking bottles.

[0036] Animal room environmental control: temperature: 18°C–29°C, relative humidity: 40%–70% RH, air exchange rate: ≥8 times / h, lighting conditions: 12 h light / 12 h dark cycle, full-spectrum fluorescent lamp.

[0037] Main instruments: electronic balance, biological safety cabinet, constant temperature culture oscillator.

[0038] Main reagents: Freund's complete adjuvant (FCA), sodium dodecyl sulfate (SDS).

[0039] 2.3 Reasons for selecting the test system The guinea pig is the preferred model for evaluating potential skin sensitization, and 2, 4-dinitrochlorobenzene (DNCB) is the positive substance recommended by the guidelines.

[0040] 2.4 Experimental design Preparation process: Samples were collected aseptically. See Table 2 for specific preparation.

[0041] Table 2

[0042] Note: The negative control solution was prepared under the same conditions except that no sample was added, and the extraction was performed under dynamic conditions.

[0043] The final extract is shown in Table 3.

[0044] Table 3

[0045] (1) The final extract should be used immediately.

[0046] (2) pH=5.19.

[0047] (3) pH=5.22.

[0048] (4) pH=5.17.

[0049] Grouping: 30 healthy adult male guinea pigs were randomly divided into groups according to the following table.

[0050] Table 4

[0051] 2.5 Test process Intradermal induction: Samples were directly injected using the stock solution. Before injection, the animal's back was shaved. Within the shaved area, each animal was injected intradermally with 0.1 mL / point as shown in Figure 1 for intradermal induction.

[0052] Figure 1 Center: 1. Head, 2. Injection area, 3. Hair removal area, 4. Tail. Injection point A: FCA: selected negative control solution (50:50 by volume) complete emulsion.

[0053] Injection point B: The experimental group was injected with the sample solution, and the control group was injected with the corresponding control solution.

[0054] Injection point C: The experimental group was injected at injection point A. Emulsifier: sample stock solution (volume ratio 50:50) emulsifier.

[0055] The control group was injected with injection point A emulsifier: corresponding control solution (volume ratio 50:50) emulsifier.

[0056] 2.6 Local induction phase Seven days after intradermal induction, local induction was performed. An absorbent gauze patch soaked in the sample solution was applied to the intradermal injection area and then sealed with medical tape. The patch was removed 48 hours after application. Control animals were treated similarly, with a patch soaked in the corresponding control solution. If the sample solution did not cause skin irritation at the intradermal injection site, 0.5 mL of 10% SDS solution was applied to the intradermal injection site 24 hours before local induction and massaged into the skin.

[0057] 2.7 Excitation phase Fourteen days after local induction, all animals were stimulated by applying absorbent gauze patches (2.5 cm × 2.5 cm) soaked in sample solution and negative control solution to the unused hairless skin of the animals, which were then sealed with medical tape and removed 24 hours after application.

[0058] 2.8 Post-excitation observation Observe the skin reactions of the animals at the provocative contact area 24 h and 48 h after removing the patch and rate them according to Table 5.

[0059] Table 7 Magnusson and Kligman classification table

[0060] 2.9 Evaluation Criteria If the rating of the test group animals is greater than or equal to 1 and the rating of the control group animals is less than 1, it indicates that the sample has a skin sensitization reaction.

[0061] If the control animals receive a rating greater than or equal to 1, but the test animals receive a rating greater than the most severe reaction in the control animals, then the sample has caused skin sensitization.

[0062] If the reaction is suspected, rechallenge is recommended to confirm the results of the first challenge.

[0063] Occasionally, more animals in the experimental group react than in the control group, but the intensity of the reaction does not exceed that of the control group. In this case, re-challenge should be performed 1 to 2 weeks after the first challenge. The method is the same as the first challenge, except that other untested parts of the animal are used.

[0064] The test results are presented as the incidence of positive challenge results in test and control animals.

[0065] 2.10 Test results The rating of the animals in the test group was less than 1, and the positive sensitization rate of the animals in the test group was 0%.

[0066] The positive sensitization rate of animals in the negative control group was 0%.

[0067] The sensitization rate of animals in the positive control group was 100%.

[0068] No abnormal clinical symptoms other than skin reactions were found in any of the animals.

[0069] It can be seen from the test results that the gel of the present application has no potential risk of irritation to the skin and is suitable as a gel for women.

[0070] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A gel composition for women containing NMN, characterized in that: The composition is prepared from the following raw materials in percentage by weight: 0.8-1.5% of carbomer 940, 0.5-1.5% of isomaltooligosaccharide, 2-4% of glycerol, 0.005-0.02% of β-nicotinamide mononucleotide (NMN), 0.001-0.01% of disodium edetate, 0.01-0.03% of ethylparaben, 0.5-1% of propylene glycol, 0.5-2% of triethanolamine, and the balance is purified water.

2. The gel composition for women containing NMN according to claim 1, characterized in that: The composition is prepared from the following raw materials in percentage by weight: 1.2% of carbomer 940, 0.5% of isomaltooligosaccharide, 2% of glycerol, 0.01% of β-nicotinamide mononucleotide (NMN), 0.003% of disodium edetate, 0.01% of ethylparaben, 0.5% of propylene glycol, 0.5% of triethanolamine, and the balance is purified water.

3. The gel composition for women containing NMN according to claim 1, characterized in that: The composition is prepared from the following raw materials in percentage by weight: 1.0% of carbomer 940, 1% of isomaltooligosaccharide, 3% of glycerol, 0.005% of β-nicotinamide mononucleotide (NMN), 0.005% of disodium edetate, 0.02% of ethylparaben, 1.0% of propylene glycol, 0.9% of triethanolamine, and the balance is purified water.

4. The gel composition for women containing NMN according to claim 1, characterized in that: The composition is prepared from the following raw materials in percentage by weight: 1.5% of carbomer 940, 1.5% of isomaltooligosaccharide, 2.5% of glycerol, 0.02% of β-nicotinamide mononucleotide (NMN), 0.01% of disodium edetate, 0.03% of ethylparaben, 1% of propylene glycol, 2.0% of triethanolamine, and the balance is purified water.

5. The gel composition for women containing NMN according to claim 1, characterized in that: The purified water is medical purified water with a maximum microbial limit of 100 cfu / ml.

6. The gel composition for women containing NMN according to claim 1, characterized in that: The pH of the gel composition is 3.8-6.

5.

7. A method for preparing the gel composition for women containing NMN according to any one of claims 1 to 6, characterized in that: The following steps are involved: Weigh a certain amount of material and set aside; Add Carbomer 940 to a container filled with purified water and stir. After it is completely dissolved, add β-nicotinamide mononucleotide (NMN), oligoisomaltodextrose, glycerol, propylene glycol, disodium EDTA, and ethylparaben to the container and stir for 4-5 hours. Add triethanolamine to adjust the pH to 3.8-6.5 and let it stand to form a gel. After the gel is sampled and tested to be qualified, it is filled by the filling machine and packaged as a finished product.

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