High throughput analysis of single molecule events
By splitting the excitation radiation beam in the optical diffraction element and irradiating the sample point with the evanescent field, the problem of overlapping excitation radiation paths and emission radiation paths in the existing technology is solved, and efficient single-molecule event high-throughput analysis and nucleic acid molecule sequence detection are achieved.
Patent Information
- Application Number
- CN202380090376.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2022-11-29
- Filing Date
- 2023-11-24
- Publication Date
- 2025-09-16
AI Technical Summary
Existing high-throughput sequencing technologies have difficulty detecting mutations in a minority of sequences present in a nucleic acid population because they are masked by the majority of sequences present in the population, and existing single-molecule analysis methods fail to effectively solve the problem of overlap between excitation radiation paths and emission radiation paths.
An optical diffraction element is used to split the excitation radiation into multiple separate radiation beams, which are then used to illuminate the sample point through the optically transparent substrate of the carrier. The excitation radiation path does not overlap with the emission radiation path. The evanescent field is used to illuminate the sample point, and the detection device captures and analyzes the emission radiation.
It achieves high-throughput analysis of single-molecule events, increases the number and flexibility of analyzable sample points, improves detection efficiency, and is suitable for sequence analysis of single nucleic acid molecules.