Beverage containing dendrobium nobile and polygonatum kingianum as well as preparation method and application of beverage

Abstract

The invention relates to a beverage containing dendrobium nobile and polygonatum kingianum as well as a preparation method and application of the beverage, and provides the beverage containing the dendrobium nobile and the polygonatum kingianum. The dendrobium officinale oral liquid is prepared from the following raw materials in parts by volume: 10 to 90 parts of dendrobium officinale slurry, 10 to 90 parts of rhizoma polygonati extracting solution, 10 to 90 parts of poria cocos extracting solution, 10 to 90 parts of herba cistanche extracting solution, 10 to 50 parts of liquorice extracting solution and 0.01 to 0.05 part of citric acid aqueous solution. According to the preparation method of the dendrobium and polygonatum kingianum beverage, five traditional Chinese medicinal materials are compounded and matched, the multifunctionality of the medicine effect can be achieved through the special formula proportion, and the synergistic effect of the medicine effects of enhancing immunity, improving fatigue and resisting oxidation and the good life cultivation and health preservation effect can be achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of pharmaceutical preparations, and in particular to a drink containing dendrobium and polygonatum, and a preparation method and application thereof. Background Art

[0002] In modern society, people's material lives have undergone significant changes. Overnutrition, lack of exercise, and environmental pollution have led to a growing number of people experiencing sub-health conditions. Consequently, demand for health and wellness continues to grow, and China has become the world's second-largest health food market and the largest consumer of these foods. People are demanding dual-purpose health foods that address both sub-health and wellness needs, while also providing no toxic side effects. Given the shared origins of both medicine and food, health products based on Traditional Chinese Medicine (TCM), such as oral solutions containing dendrobium and polygonatum, have gradually gained significant market attention. Demand for functional foods, particularly those that enhance immunity, combat fatigue, and delay aging, is on the rise.

[0003] Dendrobium officinale contains polysaccharides, phenanthrenes, bibenzyls, flavonoids, alkaloids, amino acid compounds and trace elements. These chemical components together constitute the basis of pharmacological activity; Polygonatum sibiricum contains polysaccharides, saponins, flavonoids and alkaloids. These active ingredients work together to make Polygonatum sibiricum have multiple pharmacological activities, including anti-fatigue, immune enhancement, anti-oxidation, anti-inflammatory, anti-tumor, etc.; therefore, Dendrobium officinale and Polygonatum sibiricum are often used to prepare health care products, but the health care products currently on the market, such as Dendrobium oral liquid, Polygonatum sibiricum oral liquid, Polygonatum sibiricum Poria paste, etc., are mostly prepared from single or two medicinal extracts. For example, Chinese patents with publication numbers CN111329961A and CN117918465A disclose their production processes for dendrobium beverages. Although there have been research and developments on beverages related to dendrobium officinale, and there are also technologies for preparing beverages with a composite formula of dendrobium officinale and polygonatum, such as Chinese patent publication number CN111264736A, the formulas of these beverages are too monotonous and their health functions are too single.

[0004] In order to increase the health function of the product, the product formula can be expanded and complementary Chinese medicinal materials can be added to enhance the health functionality. Summary of the Invention

[0005] In view of the above technical deficiencies, the present invention adopts a compound prescription of five Chinese medicinal materials to prepare a drink containing dendrobium and polygonatum.

[0006] On the one hand, a beverage containing Dendrobium and Polygonatum, whose raw materials are composed of the following parts by volume: 10-90 parts by volume of Dendrobium officinale slurry, 10-90 parts by volume of Polygonatum extract, 10-90 parts by volume of Poria extract, 10-90 parts by volume of Cistanche extract, 10-50 parts by volume of Licorice extract, and 0.01-0.05 parts by volume of citric acid aqueous solution.

[0007] Furthermore, a method for preparing a beverage containing dendrobium and polygonatum comprises the following steps:

[0008] Step S1, adding fresh Dendrobium officinale to deionized water, breaking the cell wall at 2-6° C., and obtaining Dendrobium officinale slurry;

[0009] Step S2, removing impurities from the polygonatum slices, deep washing with deionized water for 3-4 times, adding deionized water, and performing a first extraction, wherein the first extraction method is: heating to 40-50° C. in a closed manner, ultrasonicating for 40-50 minutes, centrifuging to obtain a first filtrate, repeating the first extraction method, centrifuging to obtain a second filtrate, adding the first filtrate, mixing, and naturally cooling to room temperature to obtain a polygonatum extract; using the above method, respectively, a Poria extract, a Cistanche extract, and a Licorice extract are obtained;

[0010] Step S3: mixing the Dendrobium officinale slurry, Polygonatum sibiricum extract, Poria cocos extract, Cistanche deserticola extract, Licorice extract and citric acid aqueous solution in proportion, and performing blending, homogenization, degassing, filling, sealing, sterilization and cooling to a finished product.

[0011] Furthermore, in step S1, the weight of the added deionized water is 8-10 times that of the Dendrobium officinale.

[0012] Furthermore, in step S1, the fresh dendrobium is 2-4 years old stems of Dendrobium huoshanense.

[0013] Furthermore, in step S1, the pressure for breaking the cell wall of Dendrobium officinale is 800-1000 MPa.

[0014] Furthermore, in step S2, ultraviolet sterilization is used throughout the entire extraction process, with the ultraviolet wavelength being 254-257 nm.

[0015] Furthermore, in step S2, the amount of deionized water added is 6-8 times the weight of the medicinal pieces.

[0016] Furthermore, in step S3, in the citric acid aqueous solution, the weight ratio of citric acid to water is 1:10-12.

[0017] Furthermore, in step S3, the sterilization method is ultraviolet sterilization, and the ultraviolet wavelength is 254-257nm.

[0018] On the other hand, drinks containing dendrobium and polygonatum are used in the preparation of health care drinks.

[0019] Huoshan Dendrobium officinale is a precious Chinese medicinal material that can be used as both medicine and food. It is sweet and slightly cold, and enters the stomach and kidneys. It nourishes yin, clears heat, benefits the stomach and produces body fluids. Huoshan Dendrobium officinale contains polysaccharides, phenanthrenes, bibenzyls, flavonoids, alkaloids, amino acid compounds and trace elements. These chemical components together constitute the basis of pharmacological activity, giving it multiple effects such as enhancing immunity, improving fatigue, anti-tumor, anti-oxidation, antibacterial, anti-inflammatory, and lowering blood sugar.

[0020] Polygonatum sibiricum is a traditional Chinese medicinal herb with both medicinal and edible properties. It is sweet and mild in nature, and benefits the spleen, lungs, and kidneys. It tonifies Qi and nourishes Yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. Polygonatum sibiricum contains polysaccharides, saponins, flavonoids, and alkaloids. These active ingredients work together to impart various pharmacological activities to Polygonatum sibiricum, including anti-fatigue, immune-enhancing, antioxidant, anti-inflammatory, and anti-tumor properties.

[0021] Poria cocos is a traditional Chinese medicine with both medicinal and edible properties. It is sweet, mild, and neutral in nature, benefiting the heart, spleen, and kidneys. It nourishes the heart and calms the mind, strengthens the spleen and stomach, and promotes diuresis and eliminates dampness. Poria cocos contains polysaccharides, triterpenoids, pachymic acid, fatty acids, proteases, dietary fiber, and trace elements, which contribute to its pharmacological effects, including relieving insomnia, regulating immunity, promoting diuresis, antibacterial and anti-tumor properties, and lowering blood sugar.

[0022] Cistanche deserticola is a traditional Chinese medicinal herb with both medicinal and edible properties. It is sweet, salty, and warm, and it nourishes the kidneys and large intestine, tonifying kidney yang, improving essence and blood, and moistening the intestines and promoting bowel movements. When combined with Dendrobium candidum and Polygonatum sibiricum, Cistanche deserticola balances yin and yang, providing nourishment without drying out. Cistanche deserticola contains phenylethanoid glycosides, iridoids, lignans, and alkaloids, which contribute to its pharmacological properties, including immune enhancement, anti-fatigue, gut microbiome regulation, antioxidant, and anti-tumor effects.

[0023] Licorice is a traditional Chinese medicinal herb that has both medicinal and edible properties. Its sweet and mild properties benefit the heart, lungs, spleen, and stomach, invigorating Qi and harmonizing the middle qi, harmonizing the medicinal properties, and alleviating the slightly cold nature of dendrobium. In this formula, licorice helps balance the medicinal properties, mitigate the harsh effects of the medication, and reduce side effects. Licorice's main components include glycyrrhizic acid, triterpenoid saponins, flavonoids, polysaccharides, phytosterols, and alkaloids, which impart various pharmacological effects, including immune regulation, antioxidant, antibacterial, anti-inflammatory, anti-tumor, cough suppressant, analgesic, and liver protection.

[0024] Beneficial effects of the present invention:

[0025] The present invention uses a compound prescription comprising Dendrobium officinale, Polygonatum sibiricum, Poria cocos, Cistanche deserticola, and Licorice. Dendrobium officinale is the main herb, while Polygonatum sibiricum is the secondary herb. The main herb, Dendrobium, is rich in polysaccharides, flavonoids, alkaloids, and other compounds, which can enhance immunity, combat fatigue, and provide antioxidant benefits. The secondary herb, Polygonatum sibiricum, also contains the same ingredients, as well as saponins, which have anti-fatigue effects. The combination of the main herb and the secondary herb works synergistically to maximize the efficacy of enhancing immunity, alleviating fatigue, and providing antioxidant benefits. Adding Poria cocos to the combination prevents the nourishing properties of the main herb, Dendrobium officinale, and Polygonatum sibiricum from affecting the digestion of the spleen and stomach, thereby achieving a nourishing effect without stagnation. Adding Cistanche deserticola balances the yin-nourishing properties of the main herb, Dendrobium officinale, and Polygonatum sibiricum, thereby achieving a yin-yang balance. Adding Licorice alleviates the slightly cold nature of the main herb, Dendrobium officinale, thereby achieving a balanced medicinal effect. At the same time, the compound compatibility takes into account the various meridians of the heart, lungs, spleen, stomach, kidneys, and large intestine. The special formula ratio of the present invention can achieve multifunctionality and comprehensive conditioning effects. The preparation process uses low-temperature extraction and ultraviolet sterilization, avoiding the loss of active ingredients of the medicinal materials caused by high-temperature extraction and sterilization, and preserving the efficacy of the medicinal materials to the greatest extent. The special formula ratio of the present invention can achieve multifunctionality of medicinal effects, thereby achieving a comprehensive conditioning effect.

[0026] The raw materials of the present invention are five kinds of Chinese medicinal materials, all of which are Chinese medicinal materials with medicinal and edible properties, are green, natural, healthy and safe, do not contain any additives, have mild tonic properties, and have no toxic side effects. At the same time, the preparation process of the present invention is simple, easy to promote, and can be packaged into small bottles for easy consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Comparison of CD3+(%), CD4+(%) and CD8+(%) in blank group, control group and experimental group;

[0028] Figure 2 Comparison of CD4+ / CD8+ among blank group, control group and experimental group;

[0029] Figure 3 This is a comparison chart of swimming time among the blank group, control group and experimental group;

[0030] Figure 4 Comparison chart of serum MDA among blank group, control group and experimental group;

[0031] Figure 5 Comparison chart of serum SOD among blank group, control group and experimental group;

[0032] Figure 6 Comparison of serum GSH-Px in blank group, control group and experimental group;

[0033] Figure 7 This is a comparison chart of tissue MDA in the blank group, control group and experimental group;

[0034] Figure 8 Comparison of tissue SOD in blank group, control group and experimental group;

[0035] Figure 9 Comparison chart of tissue GSH-Px in blank group, control group and experimental group. DETAILED DESCRIPTION

[0036] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0037] It should be noted that, in the examples, Dendrobium officinale was purchased from Huoshan Wangmei Dendrobium Development Co., Ltd., Polygonatum sibiricum slices were purchased from Jiuhua Chinese Medicinal Materials Technology Co., Ltd., Qingyang County, Anhui Province, and Poria cocos slices, Cistanche deserticola slices, and Licorice slices were all purchased from Beijing Tong Ren Tang Co., Ltd.

[0038] Example 1

[0039] 102 mL of deionized water was added to 12 g of fresh Dendrobium officinale, and the cells were disrupted at a temperature of 4 ° C and a pressure of 1000 MPa to obtain 120 mL of Dendrobium officinale slurry;

[0040] 12g of polygonatum slices were removed of impurities, washed three times with deionized water, placed in a 200mL extraction tank, and 96g of deionized water was added for the first extraction. The method for the first extraction was as follows: closed and heated to 40°C, ultrasonic-assisted extraction for 40 minutes, centrifuged to obtain 62mL of the first filtrate, repeated the first extraction method, added 58g of deionized water, and centrifuged for 20 minutes to obtain 58mL of the second filtrate. The first filtrate was added and mixed to give a total extract of 120mL. The extract was naturally cooled to room temperature to obtain a polygonatum extract. Ultraviolet sterilization was used throughout the extraction process, and the ultraviolet wavelength was 254m.

[0041] Take 12g of Poria cocos slices and use the above method to obtain 120mL of Poria cocos extract;

[0042] Take 12g of Cistanche deserticola slices and use the above method to obtain 120mL of Cistanche deserticola extract;

[0043] Take 12g of licorice slices and use the above method to obtain 120mL of licorice extract;

[0044] Take 1g of citric acid, add it to 10mL of water, stir evenly to obtain a citric acid aqueous solution;

[0045] Combine 120 mL of Dendrobium slurry, 120 mL of Polygonatum sibiricum extract, 120 mL of Poria cocos extract, 120 mL of Cistanche deserticola extract, 79.8 mL of Licorice extract, and 0.2 mL of citric acid solution to obtain 560 mL of the mixture. This mixture is then blended, homogenized, degassed, filled, sealed, sterilized, and cooled to a finished product using ultraviolet light at a wavelength of 254 nm. The finished product is packaged in 20 mL bottles.

[0046] Example 2

[0047] 102 mL of deionized water was added to 12 g of fresh Dendrobium officinale, and the cells were disrupted at 4 °C (pressure of 1000 MPa) to obtain 120 mL of Dendrobium officinale slurry;

[0048] 12g of polygonatum was removed of impurities, washed deeply with deionized water for 3 times, placed in a 200mL extraction tank, and 96g of deionized water was added for the first extraction. The method of the first extraction was as follows: closed and heated to 40°C, ultrasonic-assisted extraction for 40 minutes, centrifugal filtration, and 62mL of the first filtrate was obtained. The first extraction method was repeated, 58g of deionized water was added, and the time was 20 minutes. Centrifugal filtration was obtained to obtain 58mL of the second filtrate, the first filtrate was added, and the mixture was mixed. The total extract was 120mL, and the mixture was naturally cooled to room temperature to obtain the polygonatum extract. Ultraviolet sterilization was used throughout the extraction process, and the ultraviolet wavelength was 254m.

[0049] Take 12g of Poria cocos slices and use the above method to obtain 120mL of Poria cocos extract;

[0050] Take 12g of Cistanche deserticola slices and use the above method to obtain 120mL of Cistanche deserticola extract;

[0051] Take 12g of licorice slices and use the above method to obtain 120mL of licorice extract;

[0052] Take 1g of citric acid, add it to 10mL of water, stir evenly to obtain a citric acid aqueous solution;

[0053] Combine 100 mL of Dendrobium slurry, 120 mL of Polygonatum sibiricum extract, 120 mL of Poria cocos extract, 120 mL of Cistanche deserticola extract, 99.8 mL of Licorice extract, and 0.2 mL of citric acid solution to obtain 560 mL of the mixture. This mixture is then blended, homogenized, degassed, filled, sealed, sterilized, and cooled to a finished product using ultraviolet light at a wavelength of 254 nm. The finished product is packaged in 20 mL bottles.

[0054] Example 3

[0055] 102 mL of deionized water was added to 12 g of fresh Dendrobium officinale, and the cells were disrupted at 4 °C (pressure of 1000 MPa) to obtain 120 mL of Dendrobium officinale slurry;

[0056] 12g of polygonatum was removed of impurities, washed deeply with deionized water for 3 times, placed in a 200mL extraction tank, and 96g of deionized water was added for the first extraction. The method of the first extraction was as follows: closed and heated to 40°C, ultrasonic-assisted extraction for 40 minutes, centrifugal filtration, and 62mL of the first filtrate was obtained. The first extraction method was repeated, 58g of deionized water was added, and the time was 20 minutes. Centrifugal filtration was obtained to obtain 58mL of the second filtrate, the first filtrate was added, and the mixture was mixed. The total extract was 120mL, and the mixture was naturally cooled to room temperature to obtain the polygonatum extract. Ultraviolet sterilization was used throughout the extraction process, and the ultraviolet wavelength was 254m.

[0057] Take 12g of Poria cocos slices and use the above method to obtain 120mL of Poria cocos extract;

[0058] Take 12g of Cistanche deserticola slices and use the above method to obtain 120mL of Cistanche deserticola extract;

[0059] Take 12g of licorice slices and use the above method to obtain 120mL of licorice extract;

[0060] Take 1g of citric acid, add it to 10mL of water, stir evenly to obtain a citric acid aqueous solution;

[0061] Combine 120 mL of Dendrobium slurry, 100 mL of Polygonatum sibiricum extract, 120 mL of Poria cocos extract, 120 mL of Cistanche deserticola extract, 99.8 mL of Licorice extract, and 0.2 mL of citric acid solution to obtain 560 mL of the mixture. This mixture is then blended, homogenized, degassed, filled, sealed, sterilized, and cooled to a finished product using ultraviolet light at a wavelength of 254 nm. The finished product is packaged in 20 mL bottles.

[0062] Example 4

[0063] 102 mL of deionized water was added to 12 g of fresh Dendrobium officinale, and the cells were disrupted at 4 °C (pressure of 1000 MPa) to obtain 120 mL of Dendrobium officinale slurry;

[0064] 12g of polygonatum was removed of impurities, washed deeply with deionized water for 3 times, placed in a 200mL extraction tank, and 96g of deionized water was added for the first extraction. The method of the first extraction was as follows: closed and heated to 40°C, ultrasonic-assisted extraction for 40 minutes, centrifugal filtration, and 62mL of the first filtrate was obtained. The first extraction method was repeated, 58g of deionized water was added, and the time was 20 minutes. Centrifugal filtration was obtained to obtain 58mL of the second filtrate, the first filtrate was added, and the mixture was mixed. The total extract was 120mL, and the mixture was naturally cooled to room temperature to obtain the polygonatum extract. Ultraviolet sterilization was used throughout the extraction process, and the ultraviolet wavelength was 254m.

[0065] Take 12g of Poria cocos slices and use the above method to obtain 120mL of Poria cocos extract;

[0066] Take 12g of Cistanche deserticola slices and use the above method to obtain 120mL of Cistanche deserticola extract;

[0067] Take 12g of licorice slices and use the above method to obtain 120mL of licorice extract;

[0068] Take 1g of citric acid, add it to 10mL of water, stir evenly to obtain a citric acid aqueous solution;

[0069] Combine 100 mL of Dendrobium slurry, 100 mL of Polygonatum sibiricum extract, 120 mL of Poria cocos extract, 120 mL of Cistanche deserticola extract, 119.8 mL of Licorice extract, and 0.2 mL of citric acid solution to obtain 560 mL of the mixture. This mixture is then blended, homogenized, degassed, filled, sealed, sterilized, and cooled to a finished product using ultraviolet light at a wavelength of 254 nm. The finished product is packaged in 20 mL bottles.

[0070] 1. Testing

[0071] 100 μL of the finished product of Example 1, 100 μL of the finished product of Example 2, 100 μL of the finished product of Example 3, and 100 μL of the finished product of Example 4 were mixed with 100 μL of DPPH solution, incubated at room temperature in the dark for 30 minutes, and the absorbance was measured at 517 nm using a spectrophotometer. The sample concentration required to scaveng 50% of DPPH free radicals (IC50, in μL g / mL) was calculated. The test results are shown in the following table:

[0072] Table 1 Comparison of in vitro antioxidant activity of Examples 1-4 detected by DPPH method

[0073] sample IC50 value (μg / mL) Example 1 30.2 Example 2 61.5 Example 3 72.3 Example 4 80.7

[0074] The test results showed that Example 1 had the lowest IC50 value, reflecting that Example 1 had the strongest in vitro antioxidant activity, so Example 1 was set as the experimental group for the animal experiment.

[0075] 2. Animal Experiments

[0076] The health drinks used in the control group in the following experiments were all products prepared using the Chinese patent disclosure No. CN111264736A, and the health drinks used in the experimental group in the following experiments were all products prepared using Example 1.

[0077] The present invention conducted three animal experiments on 90 24-month-old C57BL / 6 mice (weighing 30±2g). The first animal experiment was used to detect the immune function status of the mice, the second animal experiment was used to detect the fatigue status of the mice, and the third animal experiment was used to detect the antioxidant index of the mice. Each experiment was set up with 10 blank groups, 10 control groups, and 10 experimental groups. The blank group was given deionized water only by gavage, the control group was given a control drink by gavage, and the experimental group was given the product of the present invention by gavage, once a day, 2 mL each time, for 28 consecutive days.

[0078] (1) Test of immune function status of mice:

[0079] After the last oral gavage, the subjects were fasted for 4 hours and anesthetized. Blood was collected from the orbital venous plexus and flow cytometry was used to detect peripheral blood lymphocyte T cell subsets. The test data are shown in the following table:

[0080] Table 2 CD3 in each group + (%), CD4 + (%), CD8 + (%), CD4 + / CD8 + Compare

[0081] Grouping <![CDATA[CD3 + (%)]]> <![CDATA[CD4 + (%)]]> <![CDATA[CD8 + (%)]]> <![CDATA[CD4 + / CD8 + ]]> Blank group (n=10) 25.53±4.07 13.90±2.08 14.59±3.88 0.99±0.20 Control group (n=10) 36.99±4.28 18.75±2.81 12.91±2.48 1.51±0.41 Experimental group (n=10) 40.66±2.45 22.85±2.89 8.43±1.16 2.72±0.24

[0082] like Figure 1-2 As shown, compared with the blank group and the control group, the percentages of CD3+ and CD4+ cells in the peripheral blood of mice orally administered the product of the present invention were significantly increased, the percentage of CD8+ cells was significantly decreased, and the CD4+ / CD8+ ratio was significantly increased, reflecting the immune-enhancing effect of the product of the present invention.

[0083] (2) Fatigue status test of mice:

[0084] 30 minutes after the last oral administration, a weighted exhaustive swimming test was performed. A lead sheet weighing 5% of the mouse's body weight was tied to the mouse's tail. The mouse was placed in a swimming tank at a depth of 30 cm and a temperature of 25°C. The mice were kept in a continuous swimming state throughout the experiment. The time from the start of the swim to the moment the mouse sank into the water and could not surface for 10 seconds (exhaustion) was observed and recorded. The test data for the weighted exhaustive swimming duration of the mice are shown in the following table:

[0085] Table 3 Comparison of swimming time among groups

[0086] Grouping Swimming time (min) Blank group (n=10) 5.23±0.45 Control group (n=10) 7.46±0.86 Experimental group (n=10) 10.76±0.49

[0087] like Figure 3 As shown in the results, compared with the blank group and the control group, the product of the present invention can significantly prolong the weight-bearing exhaustive swimming time of mice, reflecting the anti-fatigue effect of the product of the present invention.

[0088] (3) Antioxidant index test in mice:

[0089] The subjects were fasted for 12 hours after the last oral gavage and were anesthetized. Blood was collected from the orbital venous plexus, and the serum was separated by centrifugation (3000 rpm × 15 min) and stored at -80°C. Liver tissue was quickly removed. The test indicators and methods are as follows:

[0090] Table 4 Antioxidant index detection methods and biological significance

[0091]

[0092] The test data of mouse serum oxidative stress index are as follows:

[0093] Table 5 Comparison of serum MDA, SOD, and GSH-Px

[0094] Grouping MDA (nmol / mL) SOD (U / mL) GSH-Px (U / mL) Blank group (n=10) 5.10±0.10 14.83±4.81 145.62±10.46 Control group (n=10) 3.25±0.06 22.05±5.94 200.97±14.36 Experimental group (n=10) 1.49±0.37 29.12±5.65 254.10±13.68

[0095] The test data of mouse tissue oxidative stress index are as follows:

[0096] Table 6 Comparison of tissue MDA, SOD, and GSH-Px

[0097] Grouping MDA (nmol / mg) SOD (U / mg) GSH-Px (U / mg) Blank group (n=10) 2.93±0.04 102.54±10.04 22.40±3.51 Control group (n=10) 1.77±0.58 119.97±8.57 42.74±5.44 Experimental group (n=10) 1.10±0.58 147.76±11.20 66.24±7.01

[0098] like Figure 4-9 As shown, compared with the blank group and the control group, the MDA content in the serum and tissues of mice orally administered with the product of the present invention was significantly reduced, and the activities of SOD and GSH-Px were significantly increased, reflecting the antioxidant effect of the product of the present invention.

[0099] The above description shows the basic principles, main features and advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, from all points of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes that fall within the meaning and range of equivalents of the claims are included in the present invention. Any reference signs in the claims should not be construed as limiting the claim to which they relate.

[0100] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A beverage containing dendrobium and polygonatum, characterized in that: The raw materials are composed of the following parts by volume: 10-90 parts by volume of dendrobium officinale slurry, 10-90 parts by volume of polygonatum extract, 10-90 parts by volume of tuckahoe extract, 10-90 parts by volume of cistanche extract, 10-50 parts by volume of liquorice extract and 0.01-0.05 parts by volume of citric acid aqueous solution.

2. A method for preparing a beverage containing Dendrobium and Polygonatum as claimed in claim 1, characterized in that: The following steps are involved: Step S1, adding fresh Dendrobium officinale to deionized water, breaking the cell wall at 2-6° C., and obtaining Dendrobium officinale slurry; Step S2, removing impurities from the polygonatum slices, deep washing with deionized water for 3-4 times, adding deionized water, and performing a first extraction, wherein the first extraction method is: heating to 40-50° C. in a closed manner, ultrasonicating for 40-50 minutes, centrifuging to obtain a first filtrate, repeating the first extraction method, centrifuging to obtain a second filtrate, adding the first filtrate, mixing, and naturally cooling to room temperature to obtain a polygonatum extract; using the above method, respectively, a Poria extract, a Cistanche extract, and a Licorice extract are obtained; Step S3: mixing the Dendrobium officinale slurry, Polygonatum sibiricum extract, Poria cocos extract, Cistanche deserticola extract, Licorice extract and citric acid aqueous solution in proportion, and performing blending, homogenization, degassing, filling, sealing, sterilization and cooling to a finished product.

3. The method for preparing a beverage containing dendrobium and polygonatum according to claim 2, characterized in that: In the step S1, the weight of the added deionized water is 8-10 times that of the Dendrobium officinale.

4. The method for preparing a beverage containing dendrobium and polygonatum according to claim 2, characterized in that: In step S1, the fresh dendrobium is 2-4 years old stems of Dendrobium huoshanense.

5. The method for preparing a beverage containing dendrobium and polygonatum according to claim 2, characterized in that: In step S1, the pressure for breaking the cell wall of Dendrobium officinale is 800-1000 MPa.

6. The method for preparing a beverage containing dendrobium and polygonatum according to claim 2, characterized in that: In step S2, ultraviolet sterilization is used throughout the entire extraction process, with the ultraviolet wavelength being 254-257 nm.

7. The method for preparing a beverage containing Dendrobium and Polygonatum according to claim 2, characterized in that: In step S2, the amount of deionized water added is 6-8 times the weight of the decoction pieces.

8. The method for preparing a beverage containing Dendrobium and Polygonatum according to claim 2, characterized in that: In step S3, the weight ratio of citric acid to water in the citric acid aqueous solution is 1:10-12.

9. The method for preparing a beverage containing Dendrobium and Polygonatum according to claim 2, characterized in that: In step S3, the sterilization method is ultraviolet sterilization, and the ultraviolet wavelength is 254-257nm.

10. The beverage containing dendrobium and polygonatum according to claim 1 is used in the preparation of health care beverages.

Images