Goose hemoglobin active peptide separation and extraction method and device

Abstract

The invention relates to the technical field of hemoglobin extraction equipment, and particularly discloses a goose hemoglobin active peptide separation and extraction method and device, linkage of three functions of centrifugation, lifting and stirring is realized through single motor driving, separation stability is ensured in combination with a ratchet-pawl transmission mechanism, and multistage membrane separation is coupled through lifting motion of a separation cylinder, so that the separation efficiency is improved. The device integrates an ultrasonic module, a temperature control module and a PH adjusting module, so that the whole-process integrated operation is realized, and the technical bottlenecks of large activity loss and low efficiency of the traditional method are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of hemoglobin extraction equipment, and specifically discloses a method and device for separating and extracting goose hemoglobin active peptides. Background Art

[0002] Hemoglobin active peptides are widely used in functional foods and pharmaceuticals due to their antioxidant, antibacterial, and antihypertensive properties. As a high-quality peptide source, the extraction efficiency and activity retention of goose hemoglobin directly affect the product value. The current mainstream extraction process has the following technical bottlenecks: Disadvantages of decentralized operation on multiple devices: The traditional process requires the use of centrifuges (plasma separation), ultrasonic disruptors (cell lysis), chromatography columns (peptide separation) and other equipment in sequence. Sample transfer leads to an oxidation loss rate of 15% to 30% of active peptides. Although some devices integrate ultrasound and centrifugation functions, they do not solve the problem of dynamic control of the reaction environment (temperature, pH) during the enzymatic hydrolysis stage, and the enzymatic hydrolysis efficiency fluctuates by >20%.

[0003] Insufficient separation purity and efficiency: Membrane separation technology often uses a single ultrafiltration membrane, which is difficult to grade and remove large molecular impurities (cell debris) and small molecular salt ions, and the residual impurities in the final product are greater than 5%; During centrifugal separation, disturbance of the solid-liquid interface can easily lead to stratification and mixing, requiring the operation to be repeated 2 to 3 times, increasing the time by 50%.

[0004] At present, the separation and extraction methods of goose hemoglobin active peptides mainly include salting-out method, organic solvent precipitation method, chromatographic separation method, etc. However, these methods have some disadvantages. For example, the salting-out method is prone to introduce impurities, the organic solvent precipitation method may affect the activity of the active peptide, and the chromatographic separation method is expensive and complicated to operate. In addition, most of the existing separation and extraction devices are single-function and cannot achieve the synergistic effect of multiple separation methods, resulting in low separation efficiency and low purity of the extracted active peptides, which is difficult to meet the needs of industrial production.

[0005] Therefore, in view of this, the inventors provide a method and device for separating and extracting goose hemoglobin active peptides to solve the above problems. Summary of the Invention

[0006] The purpose of the present invention is to provide a device and method for separating and extracting goose hemoglobin active peptides. By combining centrifugal separation, ultrasonic treatment, electromagnetic stirring and a multi-stage separation structure, the efficient separation and extraction of goose hemoglobin active peptides can be achieved, the purity and activity of the active peptides can be improved, the production cost can be reduced, and the needs of industrial production can be met.

[0007] In order to achieve the above-mentioned purpose, the basic scheme of the present invention provides a method and device for separating and extracting goose hemoglobin active peptides, which include a support box, an insulation box with an ultrasonic transmitter inside, and a separation cylinder arranged in sequence from the outside to the inside. The top of the support box is provided with a blood adding tube for adding goose blood to the insulation box and an extendable suction tube fixedly connected to the separation cylinder. An electromagnetic stirring element for stirring is provided between the support box and the bottom of the insulation box. The support box and the insulation box are also connected to a number of functional tube groups for cleaning, adjusting pH, and discharging hemoglobin active peptides. A servo motor electrically connected to a controller is provided at the bottom of the support box. The servo motor extends into the insulation box and is coaxially connected to a transmission plate. It also includes a transmission member provided on the transmission plate and driven by the servo motor to centrifugally rotate the insulation cylinder, as well as a lifting member that lifts and lowers the separation cylinder to separate and extract impurities. A multi-stage separation structure is provided at the bottom of the separation cylinder.

[0008] Furthermore, the electromagnetic stirring element includes a magnetic stirrer arranged between the support box and the insulation box, and an electromagnetic stirring bar arranged in the insulation box.

[0009] Furthermore, the functional tube group includes a cleaning tube for adding physiological saline and distilled water for cleaning, an acid-base tube for adding acid-base protease for enzymatic hydrolysis, and a discharge tube for discharging hemoglobin active peptides. The support box, insulation box and separation cylinder are all made of transparent materials.

[0010] Furthermore, the inner wall of the insulation box is provided with a thermal insulation coating.

[0011] Furthermore, the transmission member includes ratchets circumferentially arranged on the transmission plate and pawls symmetrically connected to the inner wall of the insulation box and capable of abutting against the edges of the ratchets.

[0012] Furthermore, the lifting member includes a transmission rod coaxially connected to the output shaft of the servo motor and provided with an internal thread, a threaded rod threadedly connected to the transmission rod, and several connecting rods fixed to the threaded rod. The connecting rod is connected to the inner wall of the separation cylinder, and the transmission rod is detachably fixedly connected to the transmission plate.

[0013] Furthermore, the multi-stage separation structure includes a semi-permeable membrane layer and an ultrafiltration membrane layer.

[0014] Furthermore, the steps include: S001: Add goose blood and anticoagulant in a ratio of 9:1 into the incubator through a tube, start the servo motor and electromagnetic stirring to perform centrifugal rotation and stirring; S002: After stirring until the solution is separated, the separation cylinder is lowered and raised by controlling the direction of the servo motor, and the upper layer of plasma is extracted and separated during the rising process through the multi-stage separation structure, and then extracted through the suction tube; S003: The clean tube is flushed with physiological saline and distilled water, and an ultrasonic generator and a servo motor are used to perform secondary centrifugal stirring and separation to separate the solution into layers; S004: The separation cylinder is lowered and raised by controlling the direction of the servo motor again, and the cell membrane is separated and extracted from impurities through a multi-stage separation structure; S005: Add cell lysate, protease and pH-adjusting acid-base solution through the acid-base tube, and adjust the pH value to neutral using the pH detector and the acid-base solution to separate the hemoglobin active peptide and inactivate the enzyme; S006: again controlling the direction of the servo motor to lower and raise the separation cylinder, and extracting the inactivated enzyme through centrifugal separation through the multi-stage separation structure; S007: Discharge through the discharge tube and freeze, dry and prepare into a solution.

[0015] The principles and effects of this basic solution are: Multiple separation methods work synergistically to improve separation efficiency: This invention combines centrifugal separation, ultrasonic treatment, and electromagnetic stirring. Stirring and ultrasonic treatment are performed during the centrifugal rotation process to accelerate the stratification of the solution and the separation of substances. Compared with traditional single separation methods, this method greatly shortens the separation time and improves the separation efficiency. For example, in the traditional centrifugal separation process, the solution stratification may take a long time. However, the present invention uses electromagnetic stirring to make the solution more evenly mixed, and ultrasonic treatment further destroys the cell structure, accelerating the stratification process and improving the overall separation efficiency. The multi-stage separation structure ensures the purity of the active peptide: The multi-stage separation structure at the bottom of the separation cartridge, including a semipermeable membrane layer and an ultrafiltration membrane layer, filters and separates the solution multiple times, effectively removing impurities and unwanted components, and improving the purity of the hemoglobin active peptide. Compared with existing technologies, traditional separation methods may not effectively remove some small molecule impurities or large protein fragments. The multi-stage separation structure of the present invention precisely controls the molecular weight range of separation, ensuring the high purity of the extracted active peptide. Temperature control ensures the activity of active peptides: The insulation coating on the inner wall of the incubator maintains a stable internal temperature, providing an optimal temperature environment for the separation and extraction of goose hemoglobin active peptides and preventing temperature fluctuations from affecting the activity of the active peptides. In some existing technologies, temperature fluctuations can cause structural changes in the active peptides, thereby reducing their biological activity. However, the present invention effectively protects the activity of the active peptides through thermal insulation measures. Automated operation reduces costs: The device uses a controller to control the operation of components such as servo motors, automating the separation and extraction process. This reduces manual intervention, labor intensity, and labor costs. Furthermore, the high separation efficiency and high-purity extraction results reduce subsequent purification and processing costs, improving production efficiency. Transparent materials facilitate observation and control: The support box, insulation box, and separation cylinder are all made of transparent materials, allowing operators to observe the internal solution status and the separation process in real time, adjusting operating parameters promptly to ensure smooth separation and extraction. Compared to some opaque devices, this system allows operators to more intuitively understand the separation process, improving operational accuracy and controllability. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 The figure shows an overall schematic diagram of a method and device for separating and extracting goose hemoglobin active peptides proposed in an embodiment of the present application; Figure 2 A schematic diagram of a lifting component of a method and apparatus for separating and extracting goose hemoglobin active peptides proposed in an embodiment of the present application is shown; Figure 3 A schematic diagram of the transmission components of a method and device for separating and extracting goose hemoglobin active peptides proposed in an embodiment of the present application is shown; Figure 4 A schematic diagram of the multi-stage separation structure of a method and device for separating and extracting goose hemoglobin active peptides proposed in an embodiment of the present application is shown; Figure 5 A schematic diagram of the steps of a method and device for separating and extracting goose hemoglobin active peptides proposed in an embodiment of the present application is shown. DETAILED DESCRIPTION

[0018] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, features and effects of the present invention are described in detail below in conjunction with the accompanying drawings and preferred embodiments.

[0019] The figure marks in the drawings of the specification include: support box 1, insulation box 2, ratchet 201, separation cylinder 3, suction tube 4, fixed block 401, blood adding tube 5, servo motor 6, transmission rod 601, multi-stage separation structure 7, semi-permeable membrane layer 701, ultrafiltration membrane layer 702, transmission plate 8, ratchet 801, cleaning tube 9, ultrasonic transmitter 10, electromagnetic stirrer 11, magnetic stirrer 12, discharge pipe 13, connecting rod 14, threaded rod 15, acid and alkali tube 16.

[0020] A method and device for separating and extracting active peptides from goose hemoglobin, for example Figure 1As shown, the apparatus comprises, from the outside to the inside, a support box 1, an insulated box 2 equipped with an ultrasonic transmitter 10, and a separation drum 3. The top of the support box 1 is connected to a blood supply tube 5 for adding goose blood to the insulated box 2, and an extendable suction tube 4 fixedly connected to the separation drum 3. A fixed block 401 is fixedly mounted on the inner wall of the separation drum 3, and the fixed block 401 is fixedly connected to the suction tube 4, so that the suction tube 4 moves synchronously with the movement of the separation drum 3. An electromagnetic stirring element is installed between the support box 1 and the bottom of the insulated box 2 for stirring. Several functional tubes for cleaning, adjusting pH, and draining hemoglobin active peptides are also connected to the support box 1 and the insulated box 2. A servo motor 6 electrically connected to a controller is installed at the bottom of the support box 1. The servo motor 6 extends into the insulated box 2 and is coaxially connected to a transmission plate 8. The servo motor 6 also includes a transmission element mounted on the transmission plate 8, which is driven by the servo motor 6 to centrifugally rotate the insulated box, and a lifting element that raises and lowers the separation drum 3 to separate and extract impurities. A multi-stage separation structure 7 is installed at the bottom of the separation drum 3. Furthermore, the electromagnetic stirring element includes a magnetic stirrer 12 disposed between the support box 1 and the insulation box 2, and an electromagnetic stirrer 11 disposed in the insulation box 2. The magnetic stirrer 12 generates a changing magnetic field to drive the electromagnetic stirrer 11 to rotate in the insulation box 2, thereby stirring the goose blood solution and fully mixing the goose blood with the anticoagulant. At the same time, in the subsequent separation process, it helps to evenly distribute and stratify the solution.

[0021] The functional tube assembly includes a cleaning tube 9 for adding physiological saline and distilled water for cleaning, an acid-base tube 16 for adding acidic and alkaline proteases for enzymatic hydrolysis, and a discharge tube 13 for discharging hemoglobin active peptides. The support box 1, insulation box 2, and separation cylinder 3 are all made of transparent materials. The transparent design allows operators to observe the state of the internal solution and the separation process in real time. The cleaning tube 9 is used to clean the solution and remove impurities at different stages. The acid-base tube 16 is used to add cell lysis solution, protease, and acid-base solutions for pH adjustment to achieve enzymatic hydrolysis of hemoglobin and separation of active peptides. The discharge tube 13 is used to discharge the finally extracted hemoglobin active peptides out of the device.

[0022] The inner wall of the heat preservation box 2 is provided with a heat preservation coating, which can effectively reduce the heat loss in the heat preservation box 2, maintain the internal temperature stable, provide a suitable temperature environment for the separation and extraction of goose hemoglobin active peptides, and avoid the influence of temperature changes on the activity of the active peptides. like Figure 3The transmission element comprises ratchet teeth 801 circumferentially arranged on transmission plate 8 and pawls 201 symmetrically connected to the inner wall of thermal insulation box 2, capable of abutting against the edges of ratchet teeth 801. When servo motor 6 drives transmission plate 8 to rotate, the ratchet teeth 801 on transmission plate 8 interact with pawls 201, generating centrifugal force and causing thermal insulation box 2 to rotate, achieving centrifugal separation. This structural design ensures the stability and reliability of centrifugal separation.

[0023] like Figure 2 As shown, the lifting member includes a transmission rod 601 coaxially connected to the output shaft of the servo motor 6 and having an internal thread, a threaded rod 15 threadedly connected to the transmission rod 601, and a plurality of connecting rods 14 fixedly connected to the threaded rod 15. The connecting rods 14 are connected to the inner wall of the separation barrel 3. The transmission rods 601 are detachably fixedly connected to the transmission plate 8. By controlling the direction of the servo motor 6, the transmission rod 601 is driven to rotate. Due to the threaded connection between the transmission rod 601 and the threaded rod 15, the threaded rod 15 moves up and down within the transmission rod 601, thereby achieving the lifting and lowering of the separation barrel 3 through the connecting rods 14, facilitating the separation and extraction of impurities and active peptides using the multi-stage separation structure 7 at different separation stages.

[0024] like Figure 4 The multi-stage separation structure 7 includes a semi-permeable membrane layer 701 and an ultrafiltration membrane layer 702. The semi-permeable membrane layer 701 allows small molecules to pass through while retaining large molecules. The ultrafiltration membrane layer 702 further filters the solution to separate substances of different molecular weights. The synergistic effect of the semi-permeable membrane layer 701 and the ultrafiltration membrane layer 702 can effectively improve the purity of the hemoglobin active peptide.

[0025] The lifting of the separation cylinder 3 is triggered by changing the direction of the servo motor 6: Forward → centrifugal separation of plasma (speed 3000 rpm, temperature 4°C); Reverse → Separation cylinder 3 rises, and ultrafiltration membrane layer 702 intercepts impurities. Based on the above device, the present invention also provides a method for separating and extracting goose hemoglobin active peptides, the steps comprising: S001: Goose blood and anticoagulant are added to incubator 2 at a ratio of 9:1 via tube 5. Servo motor 6 and electromagnetic stirring are activated to centrifugally rotate and stir the solution. Servo motor 6 drives incubator 2 to rotate centrifugally, while the electromagnetic stirring element stirs the solution, ensuring thorough mixing of the goose blood and anticoagulant and accelerating the stratification process. S002: After stirring until the solution is separated into layers, the direction of the servo motor 6 is controlled to change, causing the separation cylinder 3 to descend and ascend. The upper layer of plasma is extracted and separated during the ascending process by the multi-stage separation structure 7, and then extracted through the suction tube 4. When the solution is separated into layers, the direction of the servo motor 6 is changed, causing the separation cylinder 3 to descend, pass through the solution layer, and then ascend again. The upper layer of plasma is separated and extracted by the multi-stage separation structure 7, and the separated plasma is extracted from the device through the suction tube 4, achieving preliminary separation of the plasma from other components. S003: Physiological saline and distilled water are introduced into the cleaning tube for flushing. The ultrasonic generator, in conjunction with the servo motor 6, performs secondary centrifugal stirring and separation to separate the solution into layers. Physiological saline and distilled water are introduced into the cleaning tube 9 to clean the remaining solution and remove any remaining impurities. Simultaneously, the ultrasonic generator emits ultrasonic waves, which, in conjunction with the centrifugal stirring of the servo motor 6, further promotes the separation of the solution into layers and improves the separation effect. S004: The servo motor 6 is again controlled to lower and raise the separation cylinder 3, and the cell membranes are separated and extracted from the impurities by the multi-stage separation structure 7. The operation of step S002 is repeated to lower and raise the separation cylinder 3, and the multi-stage separation structure 7 is used to separate and extract the cell membranes and other impurities, further purifying the solution. S005: Cell lysis solution, protease, and an acid-base solution for adjusting the pH are added through the acid-base tube 16. The pH is adjusted to neutral using a pH meter and the acid-base solution, thereby separating the hemoglobin active peptides and inactivating the enzyme. Cell lysis solution and protease are added through the acid-base tube 16 to lyse the cells and enzymatically hydrolyze the hemoglobin. The pH of the solution is monitored in real time using a pH meter. The pH is adjusted to neutral by adding acid-base solution to fully separate the hemoglobin active peptides. The enzyme is then inactivated to prevent further action of the enzyme on the active peptides. S006: The direction of the servo motor 6 is again controlled to lower and raise the separation cylinder 3, and the inactivated enzyme is extracted by centrifugation through the multi-stage separation structure 7. The direction of the servo motor 6 is changed a final time to lower and raise the separation cylinder 3, and the inactivated enzyme is separated and extracted by the multi-stage separation structure 7, thereby obtaining a relatively pure hemoglobin active peptide solution. S007: The extracted hemoglobin active peptide solution is discharged from the device through the discharge pipe 13 and then subjected to freezing and drying to remove moisture. Finally, the solution is prepared into a solution of the desired concentration for easy storage and application.

[0026] Example Prepare fresh goose blood, add goose blood and anticoagulant in a ratio of 9:1 into the incubator 2 through the tube 5, start the servo motor 6, set the speed to 1000r / min, and start the magnetic stirrer 12 at the same time, so that the electromagnetic stirrer 11 stirs the solution at a stirring speed of 200r / min, and perform centrifugal rotation and stirring for 10 minutes. After stirring until the solution is separated into layers, the controller changes the direction of the servo motor 6, causing the separation cylinder 3 to descend at a speed of 0.5 cm / s, pass through the solution layer, and then rise at the same speed. The multi-stage separation structure 7 is used to extract and separate the upper layer of plasma during the rising process, and the separated plasma is extracted from the device through the suction tube 4. Physiological saline was introduced through the cleaning tube 9 to rinse the remaining solution for 5 minutes, and then distilled water was introduced for a second rinse for 5 minutes. At the same time, the ultrasonic generator was started, the ultrasonic power was set to 200W, and the servo motor 6 was used to perform secondary centrifugal stirring and separation to separate the solution again for 15 minutes. The controller changes the direction of the servo motor 6 again to make the separation cylinder 3 descend and ascend, and the multi-stage separation structure 7 is used to separate and extract the cell membrane from the impurities, and the impurities are extracted through the suction tube 4. Cell lysate and protease were added through the acid-base tube 16, and the pH value of the solution was monitored in real time using a pH detector. The pH value was adjusted to 7.0 by adding acid-base solution to fully separate the hemoglobin active peptide, and then the enzyme was inactivated at 60°C for 10 minutes.

[0027] The controller is used to change the direction of the servo motor 6 again, so that the separation cylinder 3 descends and ascends, and the multi-stage separation structure 7 is used for centrifugal separation to extract the inactivated enzyme, which is then extracted through the suction tube 4. The extracted hemoglobin active peptide solution is discharged from the device through the discharge pipe 13, and then frozen at -20°C for 24 hours, followed by vacuum drying at 40°C for 12 hours. Finally, the dried active peptide is configured into a solution with a concentration of 10 mg / mL. The hemoglobin active peptide extracted in Example 1 was tested, and the results showed that the purity of the active peptide reached more than 95%, and the activity retention rate was 90%, which showed good separation and extraction effect. This invention breaks through the industry bottleneck of goose hemoglobin active peptide extraction through the triple technological innovation of "mechanical integrated design - separation kinetics coupling - biological activity protection". The specific effects are as follows: 1. Extraction efficiency and yield are greatly improved Single motor multi-action integration: The servo motor synchronously controls the centrifugal separation, separation drum lifting and electromagnetic stirring in forward and reverse rotation, which shortens the operation time and reduces energy consumption compared to traditional multi-device collaboration. Multi-stage membrane separation optimization: semi-permeable membrane layer intercepts large molecular impurities, ultrafiltration membrane layer intercepts active peptides, combined with lifting and centrifugation: high yield of active peptides and low impurity residues.

[0028] 2. The biological activity retention rate is high, and multiple transports are not required, and it can be completed in just one device.

[0029] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A device for separating and extracting goose hemoglobin active peptides, characterized in that: include: From the outside to the inside, there are arranged in sequence a support box, an insulation box with an ultrasonic transmitter inside, and a separation cylinder. The top of the support box is provided with a blood adding tube for adding goose blood into the insulation box and an extendable suction tube fixedly connected to the separation cylinder. An electromagnetic stirring element for stirring is provided between the support box and the bottom of the insulation box. The support box and the insulation box are also connected with a number of functional tube groups for cleaning, adjusting pH and discharging hemoglobin active peptides. The bottom of the support box is provided with a servo motor electrically connected to a controller. The servo motor extends into the insulation box and is coaxially connected to a transmission plate. It also includes a transmission member provided on the transmission plate and driven by the servo motor to centrifugally rotate the insulation cylinder, and a lifting member that lifts and lowers the separation cylinder to separate and extract impurities. A multi-stage separation structure is provided at the bottom of the separation cylinder.

2. The method and device for separating and extracting goose hemoglobin active peptide according to claim 1, characterized in that: The electromagnetic stirring element includes a magnetic stirrer arranged between the support box and the heat preservation box, and an electromagnetic stirring bar arranged in the heat preservation box.

3. The method and device for separating and extracting goose hemoglobin active peptide according to claim 1, characterized in that: The functional tube group includes a cleaning tube for adding physiological saline and distilled water for cleaning, an acid-base tube for adding acid-base protease for enzymatic hydrolysis, and a discharge tube for discharging hemoglobin active peptides. The support box, insulation box and separation cylinder are all made of transparent materials.

4. The method and device for separating and extracting goose hemoglobin active peptide according to claim 3, characterized in that: The inner wall of the heat preservation box is provided with a heat preservation coating.

5. The method and device for separating and extracting goose hemoglobin active peptide according to claim 4, characterized in that: The transmission member comprises ratchets circumferentially arranged on the transmission plate and pawls symmetrically rotatably connected to the inner wall of the heat preservation box and capable of abutting against the edges of the ratchets.

6. The method and device for separating and extracting goose hemoglobin active peptide according to claim 1, characterized in that: The lifting member includes a transmission rod coaxially connected to the output shaft of the servo motor and provided with an internal thread, a threaded rod threadedly connected to the transmission rod, and several connecting rods fixedly connected to the threaded rod. The connecting rod is connected to the inner wall of the separation cylinder, and the transmission rod is detachably fixedly connected to the transmission plate.

7. The method and device for separating and extracting goose hemoglobin active peptide according to claim 1, characterized in that: The multi-stage separation structure includes a semi-permeable membrane layer and an ultrafiltration membrane layer.

8. The method for separating and extracting active peptides from goose hemoglobin according to any one of claims 1 to 7, characterized in that: The steps include: S001: Add goose blood and anticoagulant in a ratio of 9:1 into the incubator through a tube, start the servo motor and electromagnetic stirring to perform centrifugal rotation and stirring; S002: After stirring until the solution is separated, the separation cylinder is lowered and raised by controlling the direction of the servo motor, and the upper layer of plasma is extracted and separated during the rising process through the multi-stage separation structure, and then extracted through the suction tube; S003: The clean tube is flushed with physiological saline and distilled water, and an ultrasonic generator and a servo motor are used to perform secondary centrifugal stirring and separation to separate the solution into layers; S004: The separation cylinder is lowered and raised by controlling the direction of the servo motor again, and the cell membrane is separated and extracted from impurities through a multi-stage separation structure; S005: Add cell lysis solution, protease and acid-base solution for adjusting pH value through the acid-base tube, and adjust the pH value to neutral by using the pH detector and acid-base solution to separate the hemoglobin active peptide and inactivate the enzyme; S006: again controlling the direction of the servo motor to lower and raise the separation cylinder, and extracting the inactivated enzyme through centrifugal separation through the multi-stage separation structure; S007: Discharge through the discharge tube and freeze, dry and prepare into a solution.

Images