Caulis Spatholobi extract-containing Passiflora cochinchinensis stock solution dressing with effects of promoting blood circulation to remove blood stasis, resisting inflammation, dredging collaterals and inhibiting growth of germs and application thereof

Abstract

The invention provides a caulis spatholobi extract-containing radix pseudostellariae stock solution dressing with the effects of promoting blood circulation to remove blood stasis, resisting inflammation, dredging collaterals and inhibiting growth of germs and application of the radix pseudostellariae stock solution dressing. The dressing is prepared from the following components in parts by weight: 40 to 52 parts of caulis spatholobi extract, 28 to 36 parts of radix sophorae tonkinensis extract, 18 to 24 parts of guava leaf extract, 20 to 27 parts of duchesnea indica extract, 13 to 18 parts of elephantopus scaber extract, 9 to 13 parts of polyfructose, 3 to 7 parts of carboxymethylcellulose, 2 to 5 parts of polyvinyl alcohol, 8 to 12 parts of trehalose, 6 to 11 parts of glycerinum and 200 to 300 parts of purified water. The dressing disclosed by the invention can form a layer of protective barrier on a wound surface in the use process, can effectively prevent infection and promote cell regeneration, can maintain the balance of internal and external environments of cells, effectively promotes wound healing, and can effectively inhibit growth of germs.

Description

Technical Field

[0001] The invention relates to the technical field of dressings, and in particular to a Coreopsis ginseng stock dressing containing a Millettia spatholobi extract, which has the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens, and an application thereof. Background Art

[0002] Wound infection and slow healing are common problems in clinical treatment. Traditional dressings such as gauze have defects such as poor air permeability, easy adhesion to wounds, and weak antibacterial ability. Commercially available antibacterial dressings mostly rely on chemically synthesized antibacterial agents (such as silver ions and antibiotics), which are prone to drug resistance and cytotoxicity after long-term use. Traditional Chinese medicine extracts have gradually become a hot spot in the research and development of new dressings due to their natural active ingredients and low toxicity. Existing traditional Chinese medicine extracts are used in dressings, but due to unreasonable matching, the dressings have poor effects in terms of antibacterial, anti-inflammatory and inhibition of bacterial growth. At the same time, it is also impossible to achieve long-term release of active ingredients, and the effect of a single traditional Chinese medicine extract is also limited. As a traditional Chinese medicine, Callerya speciosa has flavonoids (such as quercetin and kaempferol) and phenolic acids (such as chlorogenic acid and caffeic acid) as its main active ingredients, which have significant blood-activating, blood-stasis-removing, anti-inflammatory and antibacterial effects. Applying it in dressing materials can not only expand the application channels of Cistanche deserticola, but also is an ideal material for developing new natural medical polymer dressings. Summary of the Invention

[0003] In view of this, the present invention proposes a Coreopsis ginseng stock solution dressing containing Spatholobus suberectus extract, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and unblocking meridians, and inhibiting the growth of pathogens, and its application.

[0004] The technical solution of the present invention is achieved as follows:

[0005] The invention discloses a Coreopsis ginseng stock dressing containing a Millettia spatholobi extract, which has the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens. The dressing comprises, by weight, 40-52 parts of Millettia spatholobi extract, 28-36 parts of Sophora flavescens root extract, 18-24 parts of guava leaf extract, 20-27 parts of Duchesnea oleifera extract, 13-18 parts of Cyperus rotundus extract, 9-13 parts of polyfructose, 3-7 parts of carboxymethyl cellulose, 2-5 parts of polyvinyl alcohol, 8-12 parts of trehalose, 6-11 parts of glycerol, and 200-300 parts of purified water.

[0006] Furthermore, the invention comprises, by weight: 48 parts of Millettia spatholobi extract, 32 parts of Sophora flavescens root extract, 20 parts of guava leaf extract, 24 parts of Duchesnea oleifera extract, 13 parts of Cyperus rotundus extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 12 parts of trehalose, 8 parts of glycerol and 200 parts of purified water.

[0007] Furthermore, the preparation method of the Duchesnea indica extract is as follows: the whole Duchesnea indica herb is dried and then crushed to obtain Duchesnea powder, and then an ethanol solution is added and soaked to obtain Duchesnea slurry, and then ultrasonic extraction is repeated 3-4 times, filtered, and the filtrate is concentrated and dried to obtain the Duchesnea indica extract.

[0008] Furthermore, the material-liquid ratio of the duchesnea powder to the ethanol solution is 1:12-16 g / mL; and the concentration of the ethanol solution is 70-80 wt%.

[0009] Furthermore, the soaking temperature is 40-50° C., the soaking time is 2-3 hours, and the ultrasonic extraction temperature is 50-60° C., the ultrasonic power is 600-800 W, and the time is 12-18 minutes.

[0010] The preparation method of a dressing containing a Ceratonia spatholobi extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens comprises the following steps:

[0011] S1. Add polyvinyl alcohol and glycerin to purified water and stir and mix them evenly for the first time. Then add Millettia spatholobi extract, Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea indica extract and Herba Cynanchum multiflorum extract and continue stirring and mixing to obtain a mixed liquid.

[0012] S2. Add polyfructose, carboxymethyl cellulose and trehalose to the mixed liquid and stir and mix evenly to obtain a Coreopsis pilosula dressing containing Millettia spatholobi extract which has the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens.

[0013] Furthermore, in step S1, the initial stirring temperature is 60-80°C, the stirring speed is 80-150 r / min, and the time is 7-10 min;

[0014] Furthermore, in step S1, the stirring temperature is 60-80° C., the stirring speed is 300-400 r / min, and the stirring time is 12-16 min.

[0015] Furthermore, in step S2, the temperature of the initial stirring is 60-80°C, the stirring speed is 800-1000 r / min, and the time is 4-7 min.

[0016] The dressing of the present invention is used in preparing medicines for treating wounds, burns, ulcers and skin injuries.

[0017] Furthermore, the Cistanche deserticola of the present invention is a high-quality variety of Cistanche deserticola, and the Cistanche deserticola extract of the present invention is obtained by extracting Cistanche deserticola as a raw material.

[0018] Furthermore, the preparation method of the chicken blood vine extract is as follows: drying the chicken blood vine at 50-60°C and then crushing it to obtain chicken blood vine powder; according to the material-liquid ratio of the chicken blood vine powder to water being 1:15-18 g / mL, mixing the chicken blood vine powder with water and soaking it at 70-80°C for 50-60 minutes to obtain chicken blood vine slurry; then adding 0.1% to 0.3% of cellulase by weight of the chicken blood vine slurry; repeating ultrasonic extraction 2-3 times at a power of 300-400W and a temperature of 50-65°C, each time for 20-30 minutes, inactivating the enzyme, filtering, and taking the filtrate to obtain the chicken blood vine extract.

[0019] Furthermore, the preparation method of the chicken blood vine extract is as follows: the chicken blood vine is dried at 60°C and then crushed to obtain chicken blood vine powder, according to the solid-liquid ratio of chicken blood vine powder to water being 1:15g / mL, the chicken blood vine powder is mixed with water, and then soaked at 70°C for 60min to obtain chicken blood vine slurry, and then 0.1% of cellulase by mass of the chicken blood vine slurry is added, and ultrasonic extraction is repeated 3 times at a power of 300W and a temperature of 50°C, each time for 30min, the enzyme is inactivated, and the filtrate is filtered to obtain the chicken blood vine extract.

[0020] Furthermore, the Coreopsis ginseng stock solution dressing of the present invention refers to a dressing prepared by compounding the Cistanche spatholobi extract with other raw materials such as Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea indica extract, and Herba Cynanchum wilfordii extract.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention uses Millettia spatholobi extract, Sophora flavescens root extract, guava leaf extract, Duchesnea indica extract, Herba Lycopodii flavescentis extract, polyfructose, trehalose and the like as raw materials, which can accelerate the metabolism of local blood stasis and inhibit the release of inflammatory factors, reduce exudation and redness and swelling, and also effectively inhibit the reproduction and growth of Staphylococcus aureus, Escherichia coli, HPV virus and the like, avoid infection and aggravation of wound damage area, accelerate the growth of granulation tissue, effectively promote the repair of wound tissue, and accelerate healing.

[0023] The dressing of the present invention can form a protective barrier on the wound surface during use, can effectively prevent infection and promote cell regeneration, and can also maintain the balance of the internal and external environment of the cells, effectively promoting wound healing. DETAILED DESCRIPTION

[0024] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0025] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0026] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0027] The cellulase activity of the present invention is 2u / g, and the manufacturer is Jinhua Qianxun Biotechnology Co., Ltd.

[0028] Example 1

[0029] The preparation method of the duchesnea extract comprises the following steps: drying the whole herb of the duchesnea and then crushing it to obtain duchesnea powder; adding the duchesnea powder to a 75 wt% ethanol solution at a material-liquid ratio of the duchesnea powder to the ethanol solution of 1:12 g / mL; mixing the mixture; and soaking the mixture at 60° C. for 2 h to obtain a duchesne slurry; then repeating ultrasonic extraction three times at 60° C., an ultrasonic power of 600 W, and a time of 18 min; filtering, and concentrating and drying the filtrate to obtain the duchesnea extract.

[0030] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens. It includes, by weight: 40 parts of Millettia spatholobi extract, 28 parts of Sophora flavescens root extract, 24 parts of guava leaf extract, 20 parts of Duchesnea oleifera extract, 18 parts of Cyperus rotundus extract, 9 parts of polyfructose, 7 parts of carboxymethyl cellulose, 2 parts of polyvinyl alcohol, 11 parts of trehalose, 6 parts of glycerin, and 220 parts of purified water.

[0031] The preparation method of a dressing containing a Ceratonia spatholobi extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens comprises the following steps:

[0032] S1. Add polyvinyl alcohol and glycerol to purified water and stir for 10 min at a temperature of 80 ° C and a stirring speed of 100 r / minn to mix evenly. Then add Millettia spatholobi extract, Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea oleracea extract and Herba Cynanchifoliae extract and continue stirring for 16 min at a temperature of 80 ° C and a stirring speed of 300 r / min to mix evenly to obtain a mixed liquid;

[0033] S2. Add polyfructose, carboxymethyl cellulose and trehalose to the mixed liquid and stir at a temperature of 80° C. and a stirring speed of 800 r / min for 7 minutes to mix evenly, thereby obtaining a Coreopsis ginseng stock dressing containing Millettia spatholobi extract with the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging collaterals, and inhibiting the growth of pathogens.

[0034] Example 2

[0035] The preparation method of the duchesnea extract comprises the following steps: drying the whole herb of the duchesnea and then crushing it to obtain duchesnea powder; adding the duchesnea powder to a 70 wt% ethanol solution at a material-liquid ratio of the duchesnea powder to the ethanol solution of 1:16 g / mL; mixing the mixture; and soaking the mixture at 55° C. for 3 hours to obtain a duchesne slurry; then repeating ultrasonic extraction four times at a temperature of 60° C., an ultrasonic power of 800 W, and a time of 12 minutes; filtering, and concentrating and drying the filtrate to obtain the duchesnea extract.

[0036] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens. It includes, by weight: 52 parts of Millettia spatholobi extract, 36 parts of Sophora flavescens root extract, 18 parts of guava leaf extract, 27 parts of Duchesnea oleifera extract, 16 parts of Cyperus rotundus extract, 11 parts of polyfructose, 5 parts of carboxymethyl cellulose, 4 parts of polyvinyl alcohol, 9 parts of trehalose, 11 parts of glycerin, and 300 parts of purified water.

[0037] The preparation method of a dressing containing a Ceratonia spatholobi extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens comprises the following steps:

[0038] S1, adding polyvinyl alcohol and glycerol to purified water at a temperature of 60 ° C and a stirring speed of 80 r / minn for an initial stirring for 7 min to mix evenly, then adding Millettia spatholobi extract, Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea oleracea extract, and Herba Cynanchifoliae extract at a temperature of 60 ° C and a stirring speed of 400 r / min and continuing to stir for 12 min to mix evenly to obtain a mixed liquid;

[0039] S2. Add polyfructose, carboxymethyl cellulose and trehalose to the mixed liquid and stir at a temperature of 60° C. and a stirring speed of 1000 r / min for 4 minutes to mix evenly, thereby obtaining a Coreopsis ginseng stock dressing containing Millettia spatholobi extract with the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging collaterals, and inhibiting the growth of pathogens.

[0040] Example 3

[0041] The preparation method of the duchesnea extract comprises the following steps: drying the whole herb of the duchesnea and then crushing it to obtain duchesnea powder; adding the duchesnea powder to an ethanol solution with a concentration of 80 wt% according to a material-liquid ratio of the duchesnea powder to the ethanol solution of 1:15 g / mL; mixing the mixture; and soaking the mixture at a temperature of 50° C. for 2.5 hours to obtain a duchesne slurry; then repeating ultrasonic extraction four times at a temperature of 60° C., an ultrasonic power of 750 W, and a time of 15 minutes; filtering, and concentrating and drying the filtrate to obtain the duchesnea extract.

[0042] The Jicama ginseng stock dressing containing Millettia spatholobi extract has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens. The dressing comprises, by weight, 48 parts of Millettia spatholobi extract, 32 parts of Sophora flavescens root extract, 20 parts of guava leaf extract, 24 parts of Duchesnea oleifera extract, 13 parts of Cyperus rotundus extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 12 parts of trehalose, 8 parts of glycerin, and 200 parts of purified water.

[0043] The preparation method of a dressing containing a Ceratonia spatholobi extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens comprises the following steps:

[0044] S1. Add polyvinyl alcohol and glycerol to purified water and stir for 8 min at a temperature of 70 ° C and a stirring speed of 140 r / minn to mix evenly. Then add Millettia spatholobi extract, Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea oleracea extract and Herba Cynanchum multiflorum extract and continue stirring for 15 min at a temperature of 70 ° C and a stirring speed of 400 r / min to mix evenly to obtain a mixed liquid;

[0045] S2. Add polyfructose, carboxymethyl cellulose and trehalose to the mixed liquid and stir at a temperature of 70°C and a stirring speed of 900 r / min for 5 minutes to mix evenly, thereby obtaining a Coreopsis ginseng stock dressing containing Millettia spatholobi extract with the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and inhibiting bacterial growth.

[0046] Comparative Example 1

[0047] The difference between this comparative example and Example 3 is that the raw material ratio of the Cistanche deserticola dressing is changed, and the rest is consistent with Example 3.

[0048] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract in this comparative example, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens, comprises, by weight: 30 parts of Millettia spatholobi extract, 16 parts of Sophora flavescens root extract, 11 parts of guava leaf extract, 41 parts of Duchesnea oleifera extract, 52 parts of Cyperus rotundus extract, 5 parts of polyfructose, 1 part of carboxymethyl cellulose, 18 parts of polyvinyl alcohol, 4 parts of trehalose, 3 parts of glycerol, and 200 parts of purified water.

[0049] Comparative Example 2

[0050] The difference between this comparative example and Example 3 is that the Cerithidea chinensis stock dressing does not contain the Sophora flavescens root extract, and the rest is consistent with Example 3.

[0051] The Coreopsis ginseng stock dressing containing Cistanche deserticola extract in this comparative example, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens, comprises, by weight: 47 parts of Cistanche deserticola extract, 23 parts of guava leaf extract, 27 parts of Duchesnea oleifera extract, 16 parts of Cyperus rotundus extract, 17 parts of polyfructose, 10 parts of carboxymethyl cellulose, 9 parts of polyvinyl alcohol, 20 parts of trehalose, 12 parts of glycerol, and 200 parts of purified water.

[0052] Comparative Example 3

[0053] The difference between this comparative example and Example 3 is that the Coreopsis pilosula dressing does not contain guava leaf extract, and the rest is consistent with Example 3.

[0054] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract in this comparative example, which has the functions of promoting blood circulation and removing blood stasis, anti-inflammatory and dredging meridians, and inhibiting the growth of pathogens, comprises, by weight: 53 parts of Millettia spatholobi extract, 38 parts of Sophora flavescens extract, 29 parts of Duchesnea oleifera extract, 18 parts of Cyperus rotundus extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 11 parts of trehalose, 8 parts of glycerol, and 200 parts of purified water.

[0055] Comparative Example 4

[0056] The difference between this comparative example and Example 3 is that the Coreopsis chinensis stock dressing does not contain Duchesnea indica extract, and the rest is consistent with Example 3.

[0057] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract in this comparative example, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and unblocking meridians, and inhibiting the growth of pathogens, comprises, by weight: 54 parts of Millettia spatholobi extract, 38 parts of Sophora flavescens root extract, 26 parts of guava leaf extract, 19 parts of Cyperus rotundus extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 12 parts of trehalose, 8 parts of glycerol, and 200 parts of purified water.

[0058] Comparative Example 5

[0059] The difference between this comparative example and Example 3 is that the Cinnamomum quinquefolium liquid dressing does not contain the Herba Cynanchum wilfordii extract, and the rest is consistent with Example 3.

[0060] The Coreopsis ginseng stock dressing containing Millettia spatholobi extract in this comparative example, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and unblocking meridians, and inhibiting the growth of pathogens, comprises, by weight: 51 parts of Millettia spatholobi extract, 35 parts of Sophora flavescens root extract, 23 parts of guava leaf extract, 28 parts of Duchesnea oleifera extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 12 parts of trehalose, 8 parts of glycerol, and 200 parts of purified water.

[0061] The performance of the Cerithidea chinensis stock dressing containing the Cerithidea spatholobi extract prepared in Examples 1-3 and Comparative Examples 1-5, which has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and unblocking meridians, and inhibiting the growth of pathogens, was tested. The test process and results are as follows.

[0062] (1) Blood circulation and blood stasis removal test

[0063] Thirty-two healthy male ICR mice weighing 20-25 g were randomly divided into eight groups of four mice each. Each group of mice was intraperitoneally injected with 0.1% epinephrine (0.2 mL / mouse). 30 minutes later, the right ears of the mice were immersed in 0-4°C ice water for 10 minutes to induce microcirculatory disturbance and blood stasis in the auricle. The model was successfully established when the auricle was cyanotic and swollen. After successful modeling, the mice in each group were sprayed with 0.2 mL / mouse of the dressing prepared in Examples 1-3 and Comparative Examples 1-5, respectively, once a day, until the auricle cyanosis completely subsided and there was no swelling. The auricle was observed for damage such as exudation and ulcers, and the number of days required for the auricle cyanosis to completely subside and no swelling was recorded. The results are shown in the following table.

[0064] Table 1 Blood circulation and blood stasis removal test results

[0065]

[0066]

[0067] The above results show that the Coreopsis chinensis stock solution dressing prepared in Examples 1-3 of the present invention can accelerate the metabolism of local blood stasis and inhibit the release of inflammatory factors, reduce exudation and redness, and play a good role in promoting blood circulation and removing blood stasis. Compared with Example 3, the blood circulation and blood stasis removing effect of the Coreopsis chinensis stock solution dressing in Comparative Example 1 is reduced due to the unreasonable ratio of raw materials in the Coreopsis chinensis stock solution dressing and the lack of substances such as Sophora flavescens extract, Psidium guajava leaf extract, Duchesnea oleifera extract, and Herba Cynanchifoliae extract in Comparative Examples 1-5.

[0068] (2) Antibacterial performance test

[0069] The activated Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), and Staphylococcus epidermidis (ATCC 12228) were prepared at a concentration of 1.5×10 6CFU / mL of bacterial suspension, then take 100 μL of bacterial suspension and 100 μL of the prepared C. chinensis stock dressing containing Spatholobi extract with blood circulation and blood stasis, anti-inflammatory and antibacterial growth inhibition of Examples 1-3 and Comparative Examples 1-5 to mix to obtain a test sample solution, and after gradient dilution of each sample solution, take 0.1 mL and apply it to a plate containing tryptone soy agar (TSA), do 3 parallels for each dilution, and use the same amount of bacterial suspension and sterile PBS buffer as a positive control group, then place at 37 ° C and culture for 24-48 hours, and count the number of colonies (CFU / mL). Calculate the antibacterial rate (%) of each strain, and the results are shown in the table below.

[0070] Inhibition rate (%) = (1-average number of colonies in the experimental group / average number of colonies in the positive control group)

[0071] Table 2 Antibacterial performance

[0072]

[0073]

[0074] As shown in Table 2, the Coreopsis pilosula dressings of Examples 1-3 of the present invention have a good antibacterial effect on wound infection bacteria such as Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis. Compared with Example 3, the raw materials of the Coreopsis pilosula dressing of Comparative Example 1 are not reasonably matched, the synergistic effect of each component decreases, and the antibacterial effect also decreases. Compared with Example 3, the Coreopsis pilosula dressing raw materials of Comparative Example 2 do not add Sophora flavescens extract, the Coreopsis pilosula dressing raw materials of Comparative Example 3 do not add Guava leaf extract, the Coreopsis pilosula dressing raw materials of Comparative Example 4 do not add Duchesnea oleracea extract, and the Coreopsis pilosula dressing raw materials of Comparative Example 5 do not add Herba schoenopsis extract, and the antibacterial effect also decreases.

[0075] (3) Comparison of wound healing effects

[0076] Thirty-six healthy Kunming mice weighing 20±2g were used and divided into 9 groups with 4 mice in each group. After anesthesia, the hair on the back of the mice was removed and an incision was made on both sides of the spine of the back of the mice with an area of ​​2×2cm. 2 wounds, a concentration of 1×10 7 CFU / mL of Staphylococcus aureus solution was used to simulate infection modeling. After successful modeling, the wounds of the corresponding groups of mice were sprayed with the Coreopsis ginseng stock dressing containing Millettia reticulata extract, which has the functions of promoting blood circulation and removing blood stasis, anti-inflammatory and inhibiting bacterial growth, prepared in Examples 1-3 and Comparative Examples 1-5. The dressings were sprayed once in the morning and evening every day. The positive group was sprayed with 75% alcohol by mass every day. After spraying for n (n=) days, the wound healing was observed and the healing degree of the wounds of each group of mice was calculated. The results are shown in the following table.

[0077] Wound healing rate (%) = [(wound area on day n - initial wound area) / initial wound area] × 100%

[0078] Table 3 Wound healing status

[0079]

[0080]

[0081] The above results show that the Coreopsis chinensis stock solution dressing of Examples 1-3 of the present invention has the effect of promoting wound healing well. And from Comparative Example 1-5 compared with Example 3, it can be seen that the dressing of the present invention adopts suitable raw material matching and the synergistic effect of ingredients such as Spatholobus suberectus extract, Radix Sophorae flavescentis extract, Psidium guajava extract, Duchesnea oleifera extract, Herba Cynanchifoliae extract, polyfructose, and trehalose, which can play a synergistic effect and play a role in more effectively promoting wound healing. Explanation: The Coreopsis chinensis stock solution dressing of the present invention can form a protective barrier on the wound surface during use, can effectively prevent infection and promote cell regeneration, and can also maintain the balance of the environment inside and outside the cell, effectively promoting wound healing.

[0082] (4) Comparison of HPV virus growth inhibition performance

[0083] Human cervical cancer HeLa cells (provided by the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) were first infected with HPV 16 pseudovirions and then cultured using conventional methods. HeLa cells in the logarithmic growth phase and in good growth condition were then inoculated into a 24-well plate. After the cells adhered for 24 hours, 1 g of the Cordyceps sinensis dressing prepared in Examples 1-3 and Comparative Examples 1-5 was added, respectively. The HPV content (copies / cell) was tested 12 hours, 24 hours, 48 ​​hours, and 72 hours after intervention. The results are shown in the following table.

[0084] Table 4 Inhibition of HPV virus growth

[0085]

[0086]

[0087] The above results show that the Coreopsis chinensis liquid dressings of Examples 1-3 of the present invention have a good effect in inhibiting the growth of viruses such as HPV-16. Compared with Example 3, the inhibitory effect of Comparative Examples 1-5 on HPV growth is reduced due to the unreasonable ratio of raw materials and the lack of Sophora tonkinensis root extract, Psidium guajava leaf extract, Duchesnea oleracea extract, or Herba Cynanchifoliae extract in the dressing raw materials.

[0088] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A dressing containing a Cistanche deserticola extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens, characterized in that: The invention comprises, by weight, 40-52 parts of Millettia spatholobi extract, 28-36 parts of Sophora flavescens root extract, 18-24 parts of guava leaf extract, 20-27 parts of Duchesnea oleifera extract, 13-18 parts of Cyperus rotundus extract, 9-13 parts of polyfructose, 3-7 parts of carboxymethyl cellulose, 2-5 parts of polyvinyl alcohol, 8-12 parts of trehalose, 6-11 parts of glycerol and 200-300 parts of purified water.

2. The dressing containing the extract of Millettia spatholobi with the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 1, characterized in that: Calculated by weight, it includes: 48 parts of Millettia spatholobi extract, 32 parts of Sophora flavescens extract, 20 parts of guava leaf extract, 24 parts of Duchesnea oleifera extract, 13 parts of Cyperus rotundus extract, 13 parts of polyfructose, 6 parts of carboxymethyl cellulose, 5 parts of polyvinyl alcohol, 12 parts of trehalose, 8 parts of glycerol, and 200 parts of purified water.

3. The dressing containing the extract of Millettia spatholobi with the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 1, characterized in that: The preparation method of the Duchesnea indica extract comprises the following steps: drying the whole Duchesnea indica plant and then crushing it to obtain Duchesnea powder; then adding an ethanol solution to soak the powder to obtain Duchesnea slurry; then repeating ultrasonic extraction 3-4 times, filtering, concentrating the filtrate, and drying the filtrate to obtain the Duchesnea indica extract.

4. The dressing containing the extract of Millettia spatholobi with the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 3, characterized in that: The material-liquid ratio of the duchesnea powder to the ethanol solution is 1:12-16 g / mL; and the concentration of the ethanol solution is 70-80 wt%.

5. The dressing containing the extract of Millettia spatholobi with the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 3, characterized in that: The soaking temperature is 40-50° C. and the soaking time is 2-3 hours; the ultrasonic extraction temperature is 50-60° C., the ultrasonic power is 600-800W, and the time is 12-18 minutes.

6. The Coreopsis chinensis stock dressing containing the Cistanche deserticola extract having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to any one of claims 1 to 5, characterized in that: The preparation method of the Cerithidea chinensis stock solution dressing comprises the following steps: S1. Add polyvinyl alcohol and glycerin to purified water and stir and mix them evenly for the first time. Then add Millettia spatholobi extract, Sophora flavescens root extract, Psidium guajava leaf extract, Duchesnea indica extract and Herba Cynanchum multiflorum extract and continue stirring and mixing to obtain a mixed liquid. S2. Add polyfructose, carboxymethyl cellulose and trehalose to the mixed liquid and stir and mix evenly to obtain a Coreopsis pilosula dressing containing Millettia spatholobi extract which has the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens.

7. The dressing containing the extract of Millettia spatholobi and having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 6, characterized in that: In step S1, the temperature of the initial stirring is 60-80°C, the stirring speed is 80-150 r / min, and the time is 7-10 min; the temperature of the continued stirring is 60-80°C, the stirring speed is 300-400 r / min, and the time is 12-16 min.

8. The dressing containing the extract of Millettia spatholobi and having the effects of promoting blood circulation and removing blood stasis, resisting inflammation and unblocking meridians, and inhibiting the growth of pathogens according to claim 6, characterized in that: In step S2, the temperature of the initial stirring is 60-80°C, the stirring speed is 800-1000 r / min, and the time is 4-7 min.

9. Use of the Coreopsis chinensis stock dressing according to any one of claims 1 to 8 in the preparation of a medicament for treating wounds, burns, ulcers and skin injuries.

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