Transdermal repair composition based on plant extract and preparation method thereof

Abstract

The invention discloses a plant extract-based transdermal repair composition and a preparation method thereof, and belongs to the technical field of biology, the plant extract-based transdermal repair composition comprises glycerin, an emulsifier, sodium hyaluronate, oligopeptide-1, a plant extract eutectic compound and a proper amount of deionized water, and the glycerin, the emulsifier, the sodium hyaluronate, the oligopeptide-1 and the plant extract eutectic compound are uniformly mixed to form an ointment. According to the application, the extraction method of the polygonum cuspidatum root and the peony root bark is optimized, L-proline is added into an extraction solution to enhance the molecular polarity, glycine promotes crystallization to form nanoscale crystals, resveratrol in the polygonum cuspidatum root and paeonol in the peony root bark form a stable compound through a supramolecular eutectic technology, and the resveratrol-paeonol compound is prepared. The problems of poor light sensitivity and heat sensitivity of resveratrol and poor water solubility of paeonol are solved, and the eutectic structure prolongs the half-life period of the active component and improves the transdermal effect, thereby facilitating the effective component to play corresponding functions and improving the repair effect.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a transdermal repair composition based on plant extracts and a preparation method thereof. Background Art

[0002] The skin is the body's largest organ, sensitive to temperature, pressure, pain, and other stimuli, and possesses unique immune functions. It serves as the body's ideal "natural barrier," a "healthy armor" against disease, and the "first line of defense" for health. With the advancement of biotechnology in the field of skin repair, a growing number of researchers are utilizing ointments formulated with various plant extracts to achieve deep penetration and repair effects.

[0003] Traditional Chinese medicine plant extracts are an important source of compound ingredients. Their formulation generally involves simple mixing and stirring, with each active ingredient relying solely on its own biochemical properties to penetrate the skin and produce a repairing effect. However, some ingredients have inherent flaws, making it difficult to achieve optimal results. For example, resveratrol in Polygonum cuspidatum extract is unstable and easily degraded and inactivated during use due to its photosensitivity, heat sensitivity, and poor water solubility. Paeonol in Paeonia suffruticosa root bark extract has extremely poor water solubility and limited skin penetration, preventing it from properly performing its repairing function.

[0004] In the field, supramolecular cocrystal technology is an emerging molecular assembly method that can form stable cocrystal structures through intermolecular interactions, effectively protecting and utilizing these active ingredients. For example, patent publication number CN119454528A describes a supramolecular cocrystal compound of Ganoderma lucidum and Tibetan cypress extracts and its preparation method. This describes the co-extraction of Ganoderma lucidum and Tibetan cypress and the formation of a supramolecular cocrystal compound by controlling the extraction conditions, thus overcoming the shortcomings of using either alone and providing insights into the present invention.

[0005] Therefore, the problem to be solved by the present invention is how to provide a transdermal repair composition based on plant extracts and a preparation method thereof, improve the eutectic of the active ingredients in the root of Polygonum cuspidatum and the root bark of Paeonia suffruticosa, and improve the penetration efficiency. Summary of the Invention

[0006] To this end, the present invention provides a transdermal repair composition based on plant extracts and a preparation method thereof to solve the related technical problems existing in the prior art.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] According to a first aspect of the present invention, there is provided a transdermal repair composition based on plant extracts, comprising the following components in parts by weight:

[0009]

[0010] Add appropriate amount of deionized water and mix well to form a paste.

[0011] Furthermore, the following components are included in parts by weight:

[0012]

[0013] Add appropriate amount of deionized water and mix well to form a paste.

[0014] Furthermore, the following components are included in parts by weight:

[0015]

[0016] Add appropriate amount of deionized water and mix well to form a paste.

[0017] Furthermore, the preparation method of the plant extract eutectic compound is as follows:

[0018] Weigh the root of Polygonum cuspidatum and the root bark of Paeonia suffruticosa according to the weight ratio, mix them, and grind them together at -10°C to a particle size of 90-120 mesh using an ultrafine grinder;

[0019] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark is added to the extracting solution for ultrasonic extraction, followed by refrigerated centrifugation and filtration to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark, and the filtrate is set aside;

[0020] The filtrate was concentrated under reduced pressure, controlled at 15-20°C, vacuum degree -0.1 MPa, and rotary evaporated to obtain a crude extract;

[0021] The crude extract was stirred and redissolved in ethyl acetate-water solution with a volume ratio of 1:1, and a crystallization promoter was added. The temperature was gradually cooled from 30°C to 10°C at a cooling rate of 2°C / min to allow crystallization. The crystals were collected by filtration for later use.

[0022] The plant extract co-crystal compound was obtained by washing the crystals with cold ethanol.

[0023] Furthermore, the solid-liquid ratio of the mixed powder of the polygonum cuspidatum root and the peony root bark to the extract is 1:10, and the weight ratio of the polygonum cuspidatum root and the peony root bark is 1:1-2.

[0024] Furthermore, the extract is a 70% ethanol solution with 1.5 wt% L-proline added thereto.

[0025] Furthermore, the conditions for the ultrasonic extraction are: controlling the temperature at 15-20°C, the ultrasonic power at 300-400W, the ultrasonic frequency at 20-50kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 10-15h.

[0026] Furthermore, the addition amount of the crystal-promoting agent is 1-5 wt% of glycine.

[0027] According to a second aspect of the present invention, a method for preparing a transdermal repair composition based on a plant extract is provided, comprising the following steps:

[0028] Weigh 5-10 parts of glycerin, 2-6 parts of emulsifier, and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1;

[0029] Weigh 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2;

[0030] The ointment 2 is cooled to 10-20° C., 8-15 parts of the plant extract eutectic compound are weighed according to a certain proportion and added to the ointment 2, and mixed until uniform to obtain a transdermal repair composition.

[0031] According to a third aspect of the present invention, there is provided a use of the plant extract-based transdermal repair composition as described above in the preparation of skin ointments or cosmetics.

[0032] The present invention has the following advantages:

[0033] This application optimizes the extraction method of Polygonum cuspidatum root and Paeonia suffruticosa root bark, adds L-proline to the extract to enhance molecular polarity, promotes the formation of nano-scale crystals with glycine, and uses supramolecular eutectic technology to form a stable complex between resveratrol in Polygonum cuspidatum root and paeonol in Paeonia suffruticosa root bark, thereby solving the problems of photosensitivity and thermosensitivity of resveratrol and poor water solubility of paeonol. The eutectic structure prolongs the half-life of the active ingredient and improves the transdermal effect, thereby facilitating the effective ingredients to exert their corresponding functions and improving the repair effect. DETAILED DESCRIPTION

[0034] The following describes the implementation of the present invention using specific embodiments. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. Obviously, the embodiments described are only a portion of the present invention, not all of it. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0035] According to a first aspect of the present invention, there is provided a transdermal repair composition based on plant extracts, comprising the following components in parts by weight:

[0036]

[0037] Add appropriate amount of deionized water and mix well to form a paste.

[0038] Furthermore, the preparation method of the plant extract eutectic compound is as follows:

[0039] Weigh the root of Polygonum cuspidatum and the root bark of Paeonia suffruticosa according to the weight ratio, mix them, and grind them together at -10°C to a particle size of 90-120 mesh using an ultrafine grinder;

[0040] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark is added to the extracting solution for ultrasonic extraction, followed by refrigerated centrifugation and filtration to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark, and the filtrate is set aside;

[0041] The filtrate was concentrated under reduced pressure, controlled at 15-20°C, vacuum degree -0.1 MPa, and rotary evaporated to obtain a crude extract;

[0042] The crude extract was stirred and redissolved in ethyl acetate-water solution with a volume ratio of 1:1, and a crystallization promoter was added. The temperature was gradually cooled from 30°C to 10°C at a cooling rate of 2°C / min to allow crystallization. The crystals were collected by filtration for later use.

[0043] The plant extract co-crystal compound was obtained by washing the crystals with cold ethanol.

[0044] Furthermore, the material-liquid ratio of the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark to the extract is 1:10, and the weight ratio of Polygonum cuspidatum root and Paeonia suffruticosa root bark is 1:1-2.

[0045] Furthermore, the extract is a 70% ethanol solution with 1.5 wt% L-proline added thereto.

[0046] Furthermore, the conditions for ultrasonic extraction are: controlling the temperature at 15-20°C, the ultrasonic power at 300-400W, the ultrasonic frequency at 20-50kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 10-15h.

[0047] Furthermore, the amount of the crystal-promoting agent added is 1-5 wt% of glycine.

[0048] According to a second aspect of the present invention, a method for preparing a transdermal repair composition based on a plant extract is provided, comprising the following steps:

[0049] Weigh 5-10 parts of glycerin, 2-6 parts of emulsifier, and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1;

[0050] Weigh 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2;

[0051] The ointment 2 is cooled to 10-20° C., 8-15 parts of the plant extract eutectic compound are weighed according to a certain proportion and added to the ointment 2, and mixed until uniform to obtain a transdermal repair composition.

[0052] According to a third aspect of the present invention, there is provided a use of the above plant extract-based transdermal repair composition in the preparation of skin ointment or cosmetics.

[0053] In order to better illustrate the effects of the present application, the following examples are provided for illustration.

[0054] Preparation Example 1

[0055] The preparation method of the plant extract eutectic compound is as follows:

[0056] 100 g of Polygonum cuspidatum root and 100 g of Paeonia suffruticosa root bark were weighed and mixed according to the weight ratio, and the mixture was pulverized together with an ultrafine grinder at -10°C and passed through a 100-mesh sieve. The portion above the sieve was ultrafinely pulverized again to the target particle size.

[0057] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0058] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: controlling the temperature at 15°C, the ultrasonic power at 350W, the ultrasonic frequency at 35kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 12h.

[0059] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15° C. and vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0060] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30°C, and glycine was added at a rate of 1.5 wt% as a crystal promoter. The mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration and washed three times with cold ethanol to obtain a plant extract cocrystal weighing 23.5 g.

[0061] Preparation Example 2

[0062] The preparation method of the plant extract eutectic compound is as follows:

[0063] 100 g of Polygonum cuspidatum root and 150 g of Paeonia suffruticosa root bark were weighed and mixed according to the weight ratio, and the mixture was pulverized together with an ultrafine grinder at -10°C and passed through a 100-mesh sieve. The portion above the sieve was ultrafinely pulverized again to the target particle size.

[0064] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0065] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: controlling the temperature at 15°C, the ultrasonic power at 400W, the ultrasonic frequency at 40kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 12h.

[0066] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15° C. and vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0067] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30°C, and glycine was added at a rate of 2.5 wt% as a crystal promoter. The mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration and washed three times with cold ethanol to obtain a plant extract cocrystal weighing 28.1 g.

[0068] Preparation Example 3

[0069] The preparation method of the plant extract eutectic compound is as follows:

[0070] 100 g of Polygonum cuspidatum root and 200 g of Paeonia suffruticosa root bark were weighed and mixed according to the weight ratio, and the mixture was pulverized together with an ultrafine grinder at -10°C and passed through a 100-mesh sieve. The portion above the sieve was ultrafinely pulverized again to the target particle size.

[0071] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0072] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: controlling the temperature at 15°C, the ultrasonic power at 400W, the ultrasonic frequency at 50kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 12h.

[0073] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15° C. and vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0074] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30°C, and glycine was added at a rate of 4.5 wt% as a crystal promoter. The mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration and washed three times with cold ethanol to obtain a plant extract cocrystal weighing 22.6 g.

[0075] Comparative Example 1

[0076] The preparation method of the plant extract eutectic compound is as follows:

[0077] 150 g of peony root bark was weighed and crushed with an ultrafine grinder at -10°C and passed through a 100-mesh sieve. The portion above the sieve was ultrafinely crushed again to the target particle size.

[0078] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0079] The crushed peony root bark powder was added to 2000 mL of extracting solution for ultrasonic extraction. The ultrasonic conditions were as follows: controlling the temperature at 15°C, the ultrasonic power at 400 W, the ultrasonic frequency at 40 kHz, pausing for 10 seconds after each ultrasonic treatment for 10 seconds, and the ultrasonic treatment time for 12 hours.

[0080] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the peony root bark powder was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15° C. and a vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0081] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30°C, and glycine was added at a rate of 2.5 wt% as a crystal promoter. The mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and weighed 10.3 g.

[0082] Comparative Example 2

[0083] The preparation method of the plant extract eutectic compound is as follows:

[0084] Weigh 100 g of Polygonum cuspidatum root and grind it through a 100-mesh sieve using an ultrafine grinder at -10°C. The part above the sieve is ultrafinely grinded again to the target particle size.

[0085] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0086] The crushed root powder of Polygonum cuspidatum was added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were as follows: the temperature was controlled at 15°C, the ultrasonic power was 400 W, the ultrasonic frequency was 40 kHz, and the ultrasonic treatment time was 12 h after 10 s of pause.

[0087] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the root powder of Polygonum cuspidatum was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15°C and vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0088] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30°C, and glycine was added at a rate of 2.5 wt% as a crystal promoter. The mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration and washed three times with cold ethanol. The resulting crystals weighed 7.8 g.

[0089] Comparative Example 3

[0090] The preparation method of the plant extract eutectic compound is as follows:

[0091] 100 g of Polygonum cuspidatum root and 150 g of Paeonia suffruticosa root bark were weighed and mixed according to the weight ratio, and the mixture was pulverized together with an ultrafine grinder at -10°C and passed through a 100-mesh sieve. The portion above the sieve was ultrafinely pulverized again to the target particle size.

[0092] Preparation of the extract: First, prepare a 70% ethanol aqueous solution, weigh 30 g of L-proline, add it to the above ethanol aqueous solution, stir and mix, make the volume to 2000 mL, and cool to 15° C. for use.

[0093] The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: controlling the temperature at 15°C, the ultrasonic power at 400W, the ultrasonic frequency at 40kHz, pausing for 10s after each ultrasonic treatment for 10s, and the ultrasonic treatment time for 12h.

[0094] After the extraction, the mixture was centrifuged at 8000 rpm for 15 min, and the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark was removed by filtration. The filtrate was concentrated under reduced pressure, controlled at 15° C. and vacuum degree of -0.1 MPa, and rotary evaporated to one-fifth of the original volume to obtain a crude extract.

[0095] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring, the temperature was raised to 30°C, and the mixture was incubated with stirring for 30 minutes. Subsequently, the temperature was gradually lowered from 30°C to 10°C at a rate of 2°C / min to allow crystallization. The crude crystals were collected by filtration and washed three times with cold ethanol to obtain a plant extract cocrystal weighing 12.5 g.

[0096] Test Example 1

[0097] According to the transdermal testing experimental method and steps used in Patent Publication No. CN119454528A, the same weight of the plant extract co-crystal compounds or products of Preparation Examples 1-3 and Comparative Examples 1-3 were taken and transdermal testing was performed to verify the penetration effect of the above products. The test time was 24 hours, and samples were taken every 6 hours for testing and data was recorded. The specific test steps are not repeated here. The test results are shown in Table 1:

[0098]

[0099] Table 1 shows the permeation per unit area of ​​Preparation Examples 1-3 and Comparative Examples 1-3, and the permeation permeation increases over time. The increase in the comparative example is obviously lower than that of Preparation Examples 1-3. At the end of the experiment, the permeation permeation of Preparation Example 2 is the largest. The above experiments show that the ratio of the root of Polygonum cuspidatum and the root bark of Paeonia suffruticosa in Preparation Example 2 reaches the optimal ratio, and the generated eutectic compound is the largest, and its solubility and permeability have achieved relatively ideal results. The L-proline extract in the preparation example enhances the molecular polarity and improves the dissolution efficiency of the eutectic compound; and the addition of glycine can promote the formation of nano-scale crystals. The supramolecular eutectic technology forms a stable complex between the resveratrol in the root of Polygonum cuspidatum and the paeonol in the root bark of Paeonia suffruticosa, solving the problem of poor water solubility of resveratrol and paeonol. The plant extract eutectic compound has a good penetration effect, and is expected to achieve better use effect in terms of human skin absorption.

[0100] Through the above-mentioned Test Example 1, it has been screened that the plant extract eutectic compound prepared in Preparation Example 2 can achieve the best transmission efficiency. On this basis, the thermal stability and light stability of Preparation Example 2 are tested separately.

[0101] Test Example 2

[0102] Three portions (100 mg each) of the cocrystal compound from Preparation Example 2, standard resveratrol (100 mg each, commercially available), and standard paeonol (100 mg each, commercially available) were placed in thermostats at 25°C (control), 40°C, and 60°C, respectively. Samples were taken on days 0, 7, 14, and 28, and the resveratrol and paeonol contents were determined by HPLC (chromatographic conditions: C18 column, acetonitrile-0.1% phosphoric acid gradient elution). The results are shown in Tables 2 and 3 below:

[0103] Table 2: Resveratrol detection table

[0104]

[0105] Table 3: Paeonol detection table

[0106]

[0107]

[0108] As shown in Tables 2 and 3 above, the retention of resveratrol and paeonol in the cocrystal compound gradually decreases with increasing storage temperature, with significant degradation particularly observed above 60°C. This indicates that the cocrystal compound significantly improves the thermal stability of the ingredients and delays degradation. Furthermore, the active ingredient retention rate at 40°C for 28 days is >80%, meeting the requirements of the Cosmetic Stability Guidelines. Even at 25°C for 28 days, the retention rate remains above 90%, suggesting that storage at low temperatures is recommended to extend the shelf life.

[0109] Example 1

[0110] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0111] Weigh 5 g of glycerol, 6 g of lecithin and 0.1 g of sodium hyaluronate, dissolve them in 100 mL of deionized water, and mix until uniform; add 1.5 g of oligopeptide-1 to the above solution and mix until uniform; cool to 15°C, weigh 15 g of the plant extract eutectic compound according to the proportion and add it to the above solution, mix until uniform to obtain a transdermal repair composition.

[0112] Example 2

[0113] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0114] According to the weight of 7g of glycerol, 2g of lecithin and 0.2g of sodium hyaluronate, they were dissolved in 100mL of deionized water and mixed until uniform; 1.2g of oligopeptide-1 was added to the above solution and mixed until uniform; the temperature was lowered to 15°C, 13g of the plant extract eutectic compound was weighed according to the proportion and added to the above solution, and mixed until uniform to obtain a transdermal repair composition.

[0115] Example 3

[0116] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0117] According to the weight of 9 g of glycerol, 4 g of lecithin and 0.3 g of sodium hyaluronate, they were dissolved in 100 mL of deionized water and mixed until uniform; 0.8 g of oligopeptide-1 was added to the above solution and mixed until uniform; the temperature was lowered to 15°C, 10 g of the plant extract eutectic compound was weighed according to the proportion and added to the above solution, and mixed until uniform to obtain a transdermal repair composition.

[0118] Example 4

[0119] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0120] Weigh 8 g of glycerol, 5 g of lecithin and 0.4 g of sodium hyaluronate, dissolve them in 100 mL of deionized water, and mix until uniform; add 1 g of oligopeptide-1 to the above solution and mix until uniform; cool to 15°C, weigh 12 g of the plant extract eutectic compound according to the proportion and add it to the above solution, mix until uniform to obtain a transdermal repair composition.

[0121] Example 5

[0122] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0123] According to 10g of glycerol, 3g of lecithin and 0.5g of sodium hyaluronate, they were weighed and dissolved in 100mL of deionized water and mixed until uniform; 0.5g of oligopeptide-1 was added to the above solution and mixed until uniform; the temperature was lowered to 15°C, 8g of the plant extract eutectic compound was weighed according to the proportion and added to the above solution, and mixed until uniform to obtain a transdermal repair composition.

[0124] Comparison group 1

[0125] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, comprising the following steps:

[0126] 5 g of glycerol, 6 g of lecithin and 0.1 g of sodium hyaluronate were weighed, dissolved in 100 mL of deionized water, and mixed until uniform; 1.5 g of oligopeptide-1 was added to the above solution and mixed until uniform; the solution was cooled to 15° C. and mixed until uniform to obtain a transdermal repair composition.

[0127] Test Example 3

[0128] 30 testers were randomly divided into 6 groups. 5 of the groups were randomly assigned to use one of the transdermal repair compositions of Examples 1-5, and the remaining group was assigned to use the transdermal repair composition of Control Group 1. The testers' experience before and after use was statistically scored and graded according to the following criteria:

[0129] 1 point: no changes in skin wrinkles and spots, and no obvious changes in skin gloss;

[0130] 2 points: skin wrinkles and spots are slightly reduced, and skin glossiness is slightly improved;

[0131] 3 points: skin wrinkles and spots are significantly reduced, and skin glossiness is significantly improved;

[0132] 4 points: Skin wrinkles and spots are significantly reduced, and skin glossiness is significantly improved.

[0133] The test results are shown in Table 4 below:

[0134] Group Number of testers Average rating Example 1 5 3.7±0.3 Example 2 5 3.3±0.6 Example 3 5 2.5±0.5 Example 4 5 3.6±0.2 Example 5 5 2.8±0.5 Comparison group 1 5 1.6±0.4

[0135] As shown above, Examples 1, 2, and 4 show that, as the amount of the co-crystal compound added is different, the effect of use is improved as the amount added increases, and the effect score jumps when the amount added is ≥12%. This shows that as the amount of co-crystal compound added increases, the penetration amount increases, and it can exert a better skin repair effect. At the same time, the amount of oligopeptide-1 added in Example 1 is the largest, and it has a synergistic effect with the co-crystal compound, and the score reaches the highest score. The repair agent used in the comparative group 1 is not added with the co-crystal compound, and it is difficult to achieve the penetration effect of Examples 1, 2, and 4, resulting in a score far lower than that of the examples.

[0136] Although the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made thereto. Therefore, such modifications and improvements, without departing from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. A transdermal repair composition based on plant extracts, characterized in that: The composition comprises the following components in parts by weight: Add appropriate amount of deionized water and mix well to form a paste.

2. The transdermal repair composition based on plant extracts according to claim 1, characterized in that The composition comprises the following components in parts by weight: Add appropriate amount of deionized water and mix well to form a paste.

3. The transdermal repair composition based on plant extracts according to claim 1, characterized in that The composition comprises the following components in parts by weight: Add appropriate amount of deionized water and mix well to form a paste.

4. The transdermal repair composition based on plant extracts according to any one of claims 1 to 3, characterized in that The preparation method of the plant extract eutectic compound is as follows: Weigh the root of Polygonum cuspidatum and the root bark of Paeonia suffruticosa according to the weight ratio, mix them, and grind them together at -10°C to a particle size of 90-120 mesh using an ultrafine grinder; The crushed mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark is added to the extracting solution for ultrasonic extraction, followed by refrigerated centrifugation and filtration to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark, and the filtrate is set aside; The filtrate was concentrated under reduced pressure, controlled at 15-20°C, vacuum degree -0.1 MPa, and rotary evaporated to obtain a crude extract; The crude extract was stirred and redissolved in ethyl acetate-water solution with a volume ratio of 1:1, and a crystallization promoter was added. The temperature was gradually cooled from 30°C to 10°C at a cooling rate of 2°C / min to allow crystallization. The crystals were collected by filtration for later use. The plant extract co-crystal compound was obtained by washing the crystals with cold ethanol.

5. The transdermal repair composition based on plant extracts according to claim 4, characterized in that in, The material-liquid ratio of the mixed powder of the polygonum cuspidatum root and the peony root bark to the extract is 1:10, and the weight ratio of the polygonum cuspidatum root and the peony root bark is 1:1-2.

6. The transdermal repair composition based on plant extracts according to claim 5, characterized in that in, The extract is a 70% ethanol solution with 1.5 wt% L-proline added thereto.

7. The transdermal repair composition based on plant extracts according to claim 4, characterized in that in, The ultrasonic extraction conditions are as follows: controlling the temperature at 15-20° C., the ultrasonic power at 300-400 W, the ultrasonic frequency at 20-50 kHz, pausing for 10 seconds after each ultrasonic treatment for 10 seconds, and the ultrasonic treatment time for 10-15 hours.

8. The transdermal repair composition based on plant extracts according to claim 1, characterized in that in, The added amount of the crystal-promoting agent is 1-5 wt% of glycine.

9. A method for preparing a transdermal repair composition based on plant extracts, characterized in that: The method for preparing the transdermal repair composition based on plant extracts according to any one of claims 1 to 8 comprises the following steps: Weigh 5-10 parts of glycerin, 2-6 parts of emulsifier, and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1; Weigh 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2; The ointment 2 is cooled to 10-20° C., 8-15 parts of the plant extract eutectic compound are weighed according to a certain proportion and added to the ointment 2, and mixed until uniform to obtain a transdermal repair composition.

10. Use of the plant extract-based transdermal repair composition according to any one of claims 1 to 8 in the preparation of skin ointments or cosmetics.

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