Enzymolysis rare ginsenoside composition and application thereof in qi and blood tonifying products

Abstract

The invention provides an enzymolysis rare ginsenoside composition and application of the enzymolysis rare ginsenoside composition in qi and blood tonifying products. The composition comprises ginsenoside and astragalus polysaccharide, the mass ratio of the ginsenoside to the astragalus polysaccharide is (0.5-1.5): 2, the ginsenoside comprises ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2, and the mass ratio of the ginsenoside Compound K to the ginsenoside Rg3 to the ginsenoside Rh2 is (0.8-1.2): (0.8-1.2): 1. The ginsenoside and the astragalus polysaccharide are matched according to a proper proportion, so that a synergistic effect can be achieved in the aspect of tonifying qi and blood, the symptom of deficiency of qi and blood is effectively improved, and the qi and blood tonifying effect of the product is improved.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine, and particularly relates to an enzymatically hydrolyzed rare ginsenoside composition and its application in qi and blood-invigorating products. Background Art

[0002] Driven by traditional medicine and modern health concepts, the market demand for qi and blood tonifying products continues to grow. However, the qi and blood tonifying products currently on the market have many shortcomings and are unable to meet consumers' growing health needs.

[0003] From the perspective of ingredients, many qi and blood replenishing products still rely on the combination of traditional Chinese medicinal materials. The ingredients are complex and it is difficult to accurately control the quality and efficacy of the products, resulting in unstable effects of different batches of products.

[0004] In terms of preparation technology, traditional methods such as water decoction and alcohol extraction are inefficient, resulting in serious loss of effective ingredients, making it difficult to fully realize the potential efficacy of medicinal materials.

[0005] From the perspective of product effectiveness, existing qi and blood replenishing products often take effect slowly and require long-term and large-scale use to observe subtle improvements, which makes it difficult to meet consumers' needs for quickly relieving symptoms of qi and blood deficiency.

[0006] Therefore, it is of great significance to develop a new type of qi and blood replenishing product with scientific and reasonable ingredients, advanced and efficient preparation technology, and significant and stable effects. Summary of the Invention

[0007] The purpose of the present invention is to provide an enzymatically hydrolyzed rare ginsenoside composition, which improves the preparation efficiency and purity of rare ginsenosides by optimizing the ratio of ginsenosides to astragalus polysaccharides and the ratio between rare ginsenosides, and adopts a specific enzymatic hydrolysis process, while achieving synergistic enhancement between the various components and enhancing the efficacy of the composition in replenishing qi and blood. In addition, a qi and blood replenishing product containing the composition is provided to meet the market demand for efficient qi and blood replenishing products.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] An enzymatically hydrolyzed rare ginsenoside composition comprises ginsenosides and astragalus polysaccharides in a mass ratio of (0.5-1.5):2, wherein the ginsenosides include ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2, and the mass ratio of the ginsenoside Compound K, the ginsenoside Rg3 and the ginsenoside Rh2 is (0.8-1.2):(0.8-1.2):1.

[0010] Optionally, the ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 are obtained by hydrolyzing total ginsenosides with β-glucosidase, and the total ginsenosides include ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in a mass ratio of (2-5):(8-10):1.

[0011] Optionally, the hydrolysis temperature is 50-55° C., the pH value is 5.5-6, and the hydrolysis time is 20-24 h.

[0012] Optionally, during the hydrolysis process, the usage ratio of the β-glucosidase to the total ginsenosides is 12-15 U / mg.

[0013] Optionally, during the hydrolysis process, the usage ratio of the β-glucosidase to the total ginsenosides is 13 U / mg.

[0014] Optionally, during the hydrolysis process, the concentration of the total ginsenosides is 15-20 mg / mL.

[0015] Optionally, during the hydrolysis process, the total ginsenosides include ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in a mass ratio of 3:9:1.

[0016] Optionally, the mass ratio of the ginsenoside to the astragalus polysaccharide is 1:2.

[0017] Optionally, the mass ratio of the ginsenoside Compound K, the ginsenoside Rg3 and the ginsenoside Rh2 is 1:1:1.

[0018] A product for replenishing qi and blood, comprising the above enzymatically hydrolyzed rare ginsenoside composition.

[0019] Beneficial effects

[0020] The enzymatically hydrolyzed rare ginsenoside composition of the present invention is prepared by mixing ginsenosides and astragalus polysaccharides in a specific mass ratio (0.5-1.5):2, so that the synergistic effect of the rare ginsenoside components in the ginsenosides and the ginsenosides can better cooperate with the astragalus polysaccharides. Ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 have significant effects in enhancing the body's immunity and promoting blood circulation, while astragalus polysaccharides can regulate the function of immune cells and improve the body's anti-fatigue ability. The combination of the two in an appropriate proportion can play a synergistic role in replenishing qi and blood, more effectively improve the symptoms of qi and blood deficiency, and improve the product's qi and blood replenishing effect. DETAILED DESCRIPTION

[0021] One embodiment of the present invention provides an enzymatically hydrolyzed rare ginsenoside composition, comprising ginsenosides and astragalus polysaccharides in a mass ratio of (0.5-1.5):2, wherein the ginsenosides include ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2, and the mass ratio of ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 is (0.8-1.2): (0.8-1.2):1. In the composition, by proportioning ginsenosides and astragalus polysaccharides according to a specific mass ratio of (0.5-1.5):2, the synergistic effect of the rare ginsenoside components in the ginsenosides and the ginsenosides can better cooperate with the astragalus polysaccharide. Ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 have significant effects in enhancing the body's immunity and promoting blood circulation, while astragalus polysaccharides can regulate the function of immune cells and improve the body's anti-fatigue ability. The combination of the two in appropriate proportions can play a synergistic role in replenishing qi and blood, more effectively improve the symptoms of qi and blood deficiency, and enhance the product's qi and blood replenishing effect.

[0022] Optionally, the mass ratio of ginsenoside to astragalus polysaccharide can be 0.5:2, 0.8:2, 1:2, 1.2:2, 1.5:2, etc.

[0023] Optionally, the mass ratio of ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 is 0.8:0.8:1, 1:1:1, 1.2:1.2:1, etc.

[0024] In some embodiments, ginsenoside Compound K, ginsenoside Rg3, and ginsenoside Rh2 are obtained by hydrolyzing total ginsenosides with β-glucosidase, and the total ginsenosides include ginsenoside Rb1, ginsenoside Rc, and ginsenoside Rd in a mass ratio of (2-5): (8-10): 1. Ginsenoside Compound K, ginsenoside Rg3, and ginsenoside Rh2 are prepared by using β-glucosidase to hydrolyze total ginsenosides (ginsenoside Rb1, ginsenoside Rc, and ginsenoside Rd) in a specific mass ratio. This method can effectively improve the conversion rate and purity of rare ginsenosides, thereby ensuring the content and activity of rare ginsenosides in the composition, thereby better synergizing with astragalus polysaccharides and enhancing the qi and blood-tonifying effect of the composition.

[0025] Optionally, the mass ratio of ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd can be 2:8:1, 3:9:1, 5:10:1, etc. Preferably, the mass ratio of ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd is 3:9:1. At this time, a more suitable substrate can be provided for β-glucosidase, which is conducive to the generation of ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 with a better mass ratio. The preparation process of rare ginsenosides is further optimized from the perspective of substrates, so that the composition of rare ginsenosides in the final composition is more in line with the requirements, and the synergistic effect with ginsenosides and astragalus polysaccharides is stronger, which significantly improves the qi and blood tonifying effect of the composition.

[0026] In some embodiments, the hydrolysis temperature is 50-55°C, the pH value is 5.5-6, and the hydrolysis time is 20-24h. The hydrolysis temperature is controlled at 50-55°C, the pH value is 5.5-6, and the hydrolysis time is 20-24h. Under such conditions, β-glucosidase can maintain a high activity, so that the total ginsenosides can be fully hydrolyzed to produce rare ginsenosides. The setting of the hydrolysis conditions, on the one hand, ensures the efficient preparation of rare ginsenosides and increases the content of effective ingredients in the composition; on the other hand, suitable hydrolysis conditions help to maintain the biological activity of rare ginsenosides, so that they can better cooperate with ginsenosides and astragalus polysaccharides to enhance the efficacy of the composition in replenishing qi and blood.

[0027] As some examples of the temperature of the hydrolysis, the temperature of the hydrolysis may be 50°C, 51°C, 52°C, 53°C, 54°C, 55°C, etc.

[0028] As some examples of pH values ​​for hydrolysis, the pH value for hydrolysis can be 5.5, 5.6, 5.7, 5.8, 5.9, 6, etc.

[0029] As some examples of the hydrolysis time, the hydrolysis time may be 20 h, 21 h, 22 h, 23 h, 24 h, etc.

[0030] In some embodiments, during the hydrolysis process, the ratio of β-glucosidase to total ginsenosides is 12-15U / mg. This ratio can avoid waste of enzymes while ensuring hydrolysis efficiency, reduce production costs, and ensure that the preparation process of rare ginsenosides is both efficient and economical, and the prepared rare ginsenosides are of stable quality and can be better matched with astragalus polysaccharides to improve the overall performance of the composition. Preferably, during the hydrolysis process, the ratio of β-glucosidase to total ginsenosides is 12U / mg, 13U / mg, 14U / mg, and 15U / mg. Further preferably, during the hydrolysis process, the ratio of β-glucosidase to total ginsenosides is 13U / mg.

[0031] In some embodiments, the concentration of total ginsenosides during the hydrolysis process is 15-20 mg / mL. This concentration range allows the hydrolysis reaction to proceed in a suitable system, ensuring sufficient contact between the total ginsenosides and β-glucosidase, improving the hydrolysis effect, and further optimizing the preparation process of rare ginsenosides. This allows the prepared rare ginsenosides to better meet the requirements of the composition in terms of replenishing qi and blood, thereby enhancing the efficacy of the composition.

[0032] Another embodiment of the present invention provides a method for preparing the above-mentioned enzymatically hydrolyzed rare ginsenoside composition, comprising the following steps: uniformly mixing ginsenosides and astragalus polysaccharides.

[0033] Another embodiment of the present invention provides a product for replenishing qi and blood, comprising the above-mentioned enzymatically hydrolyzed rare ginsenoside composition.

[0034] Optionally, the Qi and blood tonifying product includes capsules, oral liquids, etc. It is understood that the capsules and oral liquids contain corresponding excipients. Optionally, the dosage of the Qi and blood tonifying product is 50-100 mg per day.

[0035] Example 1

[0036] In this embodiment, the preparation method of the enzymatically hydrolyzed rare ginsenoside composition is as follows:

[0037] S101: Weigh the total ginsenosides, including ginsenoside Rb1, ginsenoside Rc, and ginsenoside Rd, in a mass ratio of 3:9:1, and prepare a solution with a concentration of 18 mg / mL. Add β-glucosidase at a ratio of 13 U / mg to the total ginsenosides, and hydrolyze at 52°C and a pH of 5.8 for 22 hours. After the hydrolysis is completed, the ginsenosides are separated and purified to obtain ginsenosides, including ginsenoside Compound K, ginsenoside Rg3, and ginsenoside Rh2 in a mass ratio of 1:1:1.

[0038] S102: Mixing the ginsenosides prepared above with astragalus polysaccharide in a mass ratio of 1:2 to obtain an enzymatically hydrolyzed rare ginsenoside composition.

[0039] The enzymatically hydrolyzed rare ginsenoside composition is mixed with auxiliary materials to prepare capsules.

[0040] Example 2

[0041] Compared with Example 1, the difference of Example 2 is that the mass ratio of ginsenoside to astragalus polysaccharide in S102 is 0.5:2.

[0042] Example 3

[0043] Compared with Example 1, the difference of Example 3 is that the mass ratio of ginsenoside to astragalus polysaccharide in S102 is 1.5:2.

[0044] Example 4

[0045] Compared with Example 1, Example 4 is different in that the mass ratio of ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in S101 is 2:8:1.

[0046] Example 5

[0047] Compared with Example 1, the difference of Example 5 is that the mass ratio of ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in S101 is 5:10:1.

[0048] Comparative Example 1

[0049] Compared with Example 1, the difference of Comparative Example 1 is that the mass ratio of ginsenoside to astragalus polysaccharide in S102 is 0.3:2.

[0050] Comparative Example 2

[0051] Compared with Example 1, the difference of Comparative Example 2 is that the mass ratio of ginsenoside to astragalus polysaccharide in S102 is 1.8:2.

[0052] Comparative Example 3

[0053] Compared with Example 1, the difference of Comparative Example 3 is that the ginsenosides are ginsenosides obtained by extracting ginseng from ginseng using a conventional ethanol extraction method.

[0054] Blank example

[0055] In the blank example, compared with Example 1, the capsule only contained excipients.

[0056] Test Case

[0057] Mice were tested using the preparations in the Examples, Comparative Examples, and Blank Examples. The hematopoietic function indices are shown in the following table.

[0058]

[0059] It can be seen from the above table that the enzymatically hydrolyzed rare ginsenoside composition of the present invention has a significant effect of replenishing qi and blood.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

[0061] For those skilled in the art, according to the concept of the present invention, there may be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. An enzymatically hydrolyzed rare ginsenoside composition, characterized in that: It comprises ginsenosides and astragalus polysaccharides in a mass ratio of (0.5-1.5):2, wherein the ginsenosides include ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2, and the mass ratio of the ginsenoside Compound K, the ginsenoside Rg3 and the ginsenoside Rh2 is (0.8-1.2):(0.8-1.2):

1.

2. The enzymatically hydrolyzed rare ginsenoside composition according to claim 1, wherein: The ginsenoside Compound K, ginsenoside Rg3 and ginsenoside Rh2 are obtained by hydrolyzing total ginsenosides with β-glucosidase, and the total ginsenosides include ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in a mass ratio of (2-5): (8-10):

1.

3. The enzymatically hydrolyzed rare ginsenoside composition according to claim 2, wherein: The hydrolysis temperature is 50-55° C., the pH value is 5.5-6, and the hydrolysis time is 20-24 hours.

4. The enzymatically hydrolyzed rare ginsenoside composition according to claim 2, wherein: During the hydrolysis process, the dosage ratio of the β-glucosidase to the total ginsenosides is 12-15 U / mg.

5. The enzymatically hydrolyzed rare ginsenoside composition according to claim 4, wherein: During the hydrolysis process, the dosage ratio of the β-glucosidase to the total ginsenosides is 13 U / mg.

6. The enzymatically hydrolyzed rare ginsenoside composition according to claim 2, wherein: During the hydrolysis process, the concentration of the total ginsenosides is 15-20 mg / mL.

7. The enzymatically hydrolyzed rare ginsenoside composition according to claim 2, wherein: During the hydrolysis process, the total ginsenosides include ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in a mass ratio of 3:9:

1.

8. The enzymatically hydrolyzed rare ginsenoside composition according to any one of claims 1 to 7, wherein: The mass ratio of the ginsenoside to the astragalus polysaccharide is 1:

2.

9. The enzymatically hydrolyzed rare ginsenoside composition according to any one of claims 1 to 7, wherein: The mass ratio of the ginsenoside Compound K, the ginsenoside Rg3 and the ginsenoside Rh2 is 1:1:

1.

10. A product for replenishing qi and blood, characterized in that: The invention comprises the enzymatically hydrolyzed rare ginsenoside composition according to any one of claims 1 to 9.

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