Application of turpinia arguta leaf superfine powder in preparation of riemerella anatipestifer disease prevention product

Abstract

The invention relates to the field of poultry medicines and poultry medicine preparation, and particularly discloses application of turpinia arguta leaf superfine powder in preparation of a riemerella anatipestifer disease prevention product. The turpinia arguta leaf superfine powder provided by the invention has the effects of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation and the like; the traditional Chinese medicine superfine powder is simple to prepare, low in cost, high in safety and free of side effects, and can reduce or replace the use of antibiotics, so that the problems of drug resistance and drug residues are reduced; by using the turpinia arguta leaf superfine powder disclosed by the invention, the riemerella anatipestifer disease of the meat ducks can be remarkably prevented.

Description

Technical Field

[0001] The invention relates to the field of poultry medicine and poultry medicine preparation, and particularly relates to application of osmanthus fragrans leaf ultrafine powder in the preparation of a product for preventing Riemerella anatipestifer disease. Background Art

[0002] Riemerella anatipestifer disease is a chronic or acute septicemic infectious disease caused by Riemerella anatipestifer, which mainly infects ducklings. It is easily infected in ducklings aged 1 to 8 weeks and can infect poultry such as chickens and geese. There are many serotypes of Riemerella anatipestifer strains, and there is a lack of cross-immunity between the serotypes. In order to reduce the use of antibiotics and reduce bacterial resistance, we should start with the research and development of highly effective, safe and inexpensive antibacterial drugs. The leaves of Rhizoma Cibotii have multiple effects such as anti-inflammatory, antioxidant and immune-enhancing, making them a potential choice. However, Chinese herbal medicines face problems such as high extraction cost and low oral absorption rate in clinical applications. To solve this problem, the present invention selects high-quality Rhizoma Cibotii raw materials and makes them into ultrafine powder through modern processing technology, which reduces production costs while improving the absorption and utilization of drugs. Summary of the Invention

[0003] Purpose of the invention: The technical problem to be solved by the present invention is to address the deficiencies of the existing technology and provide an application of ultrafine powder of Scutellaria baicalensis leaves in the preparation of products for preventing Riemerella anatipestifer.

[0004] In order to solve the above technical problems, the present invention discloses the following technical solutions: In a first aspect, the present invention discloses an excellent traditional Chinese medicine capable of preventing and / or treating Riemerella anatipestifer disease and resisting Riemerella anatipestifer infection: Fructus serratae leaves.

[0005] This Chinese herbal medicine belongs to the plant kingdom, angiosperms, dicots, Sapindus orders, Sapindaceae, genus Cyperus, and species Cyperus acuminata.

[0006] The pharmacological activities of the leaves of Rhizoma Cynanchum are as follows: nature and flavor: bitter, cold, and enter the lung and liver meridians.

[0007] Main functions: clearing away heat and detoxifying, relieving sore throat and swelling, promoting blood circulation and relieving pain, antibacterial and anti-inflammatory. Used for sore throat, sore throat, sore ulcers, swelling and pain, and pain from falls.

[0008] In a second aspect, the present invention discloses ultrafine powder of Cyperus rotundus leaves and a preparation method thereof.

[0009] Wherein, the particle size of the Scutellaria baicalensis leaf ultrafine powder is less than 75 μm.

[0010] The method for preparing the ultrafine powder of the leaves of the safflower oil plant comprises the following steps: (1) Pretreatment of medicinal materials: Purchase the leaves of the Chinese fragrant osmanthus, clean them, drain them and use a low-temperature slow drying method to prevent the destruction of the active ingredients. Initial drying is at 45-50℃ for 24 hours; the main drying is at 56-60℃ for 40-48 hours; the final drying is at 50℃ for 12 hours. The final moisture content is ≤8%, and the leaves are easy to break without being charred.

[0011] (2) Medicinal material crushing: The dried Scutellaria baicalensis leaves were crushed for the first time using a small leaf crusher, and then crushed three times using an industrial universal crusher for 2 minutes each time; and then ultrafine crushed using a 200-500 mesh air flow ultrafine powder crusher.

[0012] (3) Medicinal material packaging: Weigh 1 kg of ultrafine powder and use a vacuum packaging machine to package.

[0013] In a third aspect, the present invention discloses the use of the Cyperus rotundus leaf described in the first aspect and the Cyperus rotundus leaf ultrafine powder described in the second aspect.

[0014] Wherein, the application is application in preparing products for preventing and / or treating Riemerella anatipestifer disease.

[0015] Wherein, the Riemerella anatipestifer disease is Riemerella anatipestifer disease caused by Riemerella anatipestifer.

[0016] Wherein, the prevention and / or treatment of Riemerella anatipestifer disease includes any one of the following: i. Alleviating any one or a combination of serositis, liver swelling and effusion in ducks caused by Riemerella anatipestifer; the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis; ii. Reduce mortality caused by Riemerella anatipestifer; iii. reducing any one or a combination of white diarrhea, depression and nervous symptoms caused by Riemerella anatipestifer infection; iv, reducing weight loss caused by Riemerella anatipestifer; v. Reducing any one or a combination of the bacterial load in the heart, liver and brain tissue caused by Riemerella anatipestifer infection.

[0017] Among them, when the application is for prevention, the ultrafine powder of Scutellaria baicalensis leaves is directly fed every day, with a dosage of 2-2.5 g / kg body weight, preferably, the dosage is 2.3 g / kg body weight, which can be taken in divided doses or all at once; or the ultrafine powder of Scutellaria baicalensis leaves with a mass fraction of 1-10wt% is added to the feed.

[0018] The application can also be in the preparation of products for resisting Riemerella anatipestifer infection.

[0019] Wherein, the resistance to Riemerella anatipestifer infection includes any one of the following: 1) Alleviating any one or a combination of serositis, liver swelling and effusion in ducks caused by Riemerella anatipestifer infection; the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis; 2) Reduce mortality caused by Riemerella anatipestifer infection; 3) Reducing any one or a combination of white diarrhea, depression and neurological symptoms caused by Riemerella anatipestifer infection; 4) Reduce weight loss caused by Riemerella anatipestifer infection; 5) Reducing any one or a combination of the bacterial load in the heart, liver, and brain tissue caused by Riemerella anatipestifer infection.

[0020] In a fourth aspect, the present invention discloses a product comprising the Cyperus rotundus leaf described in the first aspect or the Cyperus rotundus leaf ultrafine powder described in the second aspect.

[0021] Wherein, the product is a product for preventing and / or treating Riemerella anatipestifer disease.

[0022] Wherein, the Riemerella anatipestifer disease is Riemerella anatipestifer disease caused by Riemerella anatipestifer.

[0023] Wherein, the prevention and / or treatment of Riemerella anatipestifer disease includes any one of the following: i. Alleviating any one or a combination of serositis, liver swelling and effusion in ducks caused by Riemerella anatipestifer; the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis; ii. Reduce mortality caused by Riemerella anatipestifer; iii. reducing any one or a combination of white diarrhea, depression and nervous symptoms caused by Riemerella anatipestifer infection; iv, reducing weight loss caused by Riemerella anatipestifer; v. Reducing any one or a combination of the bacterial load in the heart, liver and brain tissue caused by Riemerella anatipestifer infection.

[0024] The product can also be a product for resisting Riemerella anatipestifer infection.

[0025] Wherein, the resistance to Riemerella anatipestifer infection includes any one of the following: 1) Alleviating any one or a combination of serositis, liver swelling and effusion in ducks caused by Riemerella anatipestifer infection; the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis; 2) Reduce mortality caused by Riemerella anatipestifer infection; 3) Reducing any one or a combination of white diarrhea, depression and neurological symptoms caused by Riemerella anatipestifer infection; 4) Reduce weight loss caused by Riemerella anatipestifer infection; 5) Reducing any one or a combination of the bacterial load in the heart, liver, and brain tissue caused by Riemerella anatipestifer infection.

[0026] The dosage form of the product is an oral dosage form, including oral liquid, granules, powder, powder, pills, tablets or capsules.

[0027] The safflower leaf or safflower leaf ultrafine powder provided by the present invention is an excellent traditional Chinese veterinary medicine that can be used to replace or reduce the use of antibiotics in preventing Riemerella anatipestifer. The safflower leaf ultrafine powder provided by the present invention can also improve the absorption effect and efficacy of traditional Chinese medicine.

[0028] The products described in the present invention include feed, feed additives or veterinary drugs; for example, the Scutellaria baicalensis leaves or Scutellaria baicalensis leaves ultrafine powder can be added to the feed in an amount of 1-10wt%, such as 2wt%, 3wt%, 4wt%, 5wt%, or 8wt%, thereby reducing or replacing antibiotics and significantly preventing infection with Riemerella anatipestifer.

[0029] The Cyperus rotundus leaf or Cyperus rotundus leaf superfine powder of the present invention can be taken by ducklings when they are 4 to 18 days old.

[0030] Beneficial effects: Compared with the prior art, the present invention has the following advantages: (1) The ultrafine powder of the leaves of the osmanthus fragrans provided by the present invention has the effects of clearing away heat and detoxifying, antibacterial, anti-inflammatory, and is not prone to drug resistance.

[0031] (2) The ultrafine powder of the leaves of the Scutellaria baicalensis provided by the present invention is safe, has significant efficacy, is easy to use, and is a Chinese herbal medicine ultrafine powder for preventing Riemerella anatipestifer infection in meat ducks.

[0032] (3) The ultrafine powder of Osmanthus fragrans leaves provided by the present invention has a significant preventive effect and does not require injection or oral administration of antibiotics. It can reduce or replace antibiotics in clinical disease prevention.

[0033] (4) The leaves of the safflower provided by the present invention are ultrafine powders with a high cell wall breaking rate, thereby improving the dissolution rate and bioavailability of the active ingredients.

[0034] (5) Ultrafine powder can be directly absorbed through the intestinal mucosa, increasing bioavailability, reducing damage to medicinal materials, lowering medication costs, and facilitating storage and transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more apparent.

[0036] Figure 1 This is the autopsy diagram of the challenge group in Example 1.

[0037] Figure 2 This is the autopsy diagram of the prevention group of the Cyperus rotundus in Example 1.

[0038] Figure 3 2 is a comparison chart of feces in each group in Example 2.

[0039] Figure 4 2 is a comparison chart of weight changes in each group in Example 2.

[0040] Figure 5 This is a comparative diagram of the liver anatomy of each group at 1 dpi in Example 2.

[0041] Figure 6 This is a comparative diagram of the anatomy of the heart in each group at 1 dpi in Example 2.

[0042] Figure 7 This is a comparison diagram of the anatomy of each group at 4 dpi in Example 2.

[0043] Figure 8 This is a comparison diagram of the anatomy of each group at 7 dpi in Example 2.

[0044] Figure 9 This is a comparison chart of the bacterial load of target organs in Example 2.

[0045] Figure 10 This is an anatomical diagram of the clinical preventive application of the superfine powder of Cyperus rotundus leaves in Example 3.

[0046] Figure 11 This is an anatomical diagram of the clinical production without preventive treatment in Example 3. DETAILED DESCRIPTION

[0047] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0048] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.

[0049] The particle size of the ultrafine powder of Scutellaria baicalensis leaves described in the following examples is less than 75 μm.

[0050] In the present invention, dpi refers to the number of days after poison challenge, such as 1 dpi refers to 1 day after poison challenge.

[0051] Example 1 1. Materials and Methods 1.1 Experimental Animals Sixty one-day-old Mallard ducks were purchased from Nanchang, Jiangxi Province, between June and July 2024. All ducklings were healthy and tested for Riemerella anatipestifer, with negative results.

[0052] 1.2. Investigational Drugs Ultrafine powder of Cyperus rotundus leaf.

[0053] 1.3 Test methods The ducklings were randomly divided into two groups: the Thunbergia rotundifolia prevention group (TF+RA) and the Riemerella anatipestifer challenge group (RA), with 30 ducks in each group.

[0054] The one-day-old ducks in the prevention group were fed with the leaf of Scutellaria serrata for 3 days. Then, 1% of the ultrafine powder of Scutellaria serrata was added to the feed on the 4th day of age until the end of the experiment. During the experiment, the ducks were allowed to drink water and eat freely. The ducks were challenged with the virus at the 11th day of age. The concentration of the challenged bacteria was 2.15×10 10 CFU / mL, after bacterial recovery, centrifuge at 3500r / min for 10min, discard the culture medium, and resuspend in normal saline. The infection method is intraperitoneal injection of 1mL.

[0055] The R. anatipestifer challenge group was fed a basal diet until the end of the experiment. During the experiment, they had free access to water and food. On the 11th day of age, the challenge was performed with a bacterial concentration of 2.15×10 10 CFU / mL, after bacterial recovery, centrifuge at 3500r / min for 10min, discard the culture medium, and resuspend in normal saline. The infection method is intraperitoneal injection of 1mL.

[0056] The mortality rate was calculated 7 days after infection, and the fibrinous inflammatory changes in the heart and liver were observed after autopsy.

[0057] 2. Test results 2.1 Mortality As shown in Table 1, 12 animals died in the challenge group, with a mortality rate of 40%; 3 animals died in the Scutellaria serrata leaf prevention group, with a mortality rate of 10%. Adding the Scutellaria serrata leaf ultrafine powder of the present invention to the feed can significantly reduce the mortality rate of Riemerella anatipestifer infection.

[0058] Table 1 Number of deaths in ducks infected with Riemerella anatipestifer in the challenge group and the Scutellaria serrata leaf prevention group

[0059] 2.2 Autopsy changes On the 8th day after the challenge, 10 ducks were randomly selected for autopsy. Figure 1 and Figure 2 The red circle indicates fibrinous pericarditis, and the yellow circle indicates fibrinous perihepatitis. Nine animals in the challenge group developed fibrinous pericarditis, and five developed fibrinous perihepatitis. In the preventive group, three developed fibrinous pericarditis, and one developed fibrinous perihepatitis. Adding ultrafine powder of the leaves of the osmanthus fragrans to feed significantly reduced fibrinous pericarditis and fibrinous perihepatitis caused by Riemerella anatipestifer infection.

[0060] Example 2 1. Materials and Methods 1.1 Experimental Animals 200 one-day-old Mallard ducks were purchased from Nanchang, Jiangxi Province from August to September 2024. All ducklings were healthy and tested for Riemerella anatipestifer, and the results were negative.

[0061] 1.2. Investigational Drugs Ultrafine powder of Cyperus rotundus leaf.

[0062] 1.3 Test methods Two hundred one-day-old ducks were purchased and tested for the pathogen Riemerella anatipestifer. If the test was negative, they were adaptively fed a basal diet for three days. On day four, they were divided into a basal diet group and a group supplemented with 1wt% of R. anatipestifer leaf in their feed, with 100 ducks in each group. On day 10, the basal diet group was divided into a control group (C) of 50 and a challenge group (RA) of 50. The group supplemented with 1wt% of R. anatipestifer leaf in their feed was divided into a R. anatipestifer control group (C+TF) of 50 and a R. anatipestifer preventive group (RA+TF) of 50.

[0063] The control group (Group C) was fed with a basal diet until the end of the experiment. During the experiment, the rats were fed with food and water ad libitum. On the 11th day of age, 1 mL of normal saline was injected intraperitoneally.

[0064] The challenge group (RA group) was fed with a basal diet until the end of the experiment. During the experiment, they had free access to food and water. On the 11th day of age, they were challenged with a concentration of 2.15×10 10 After bacterial recovery, the culture medium was centrifuged at 3500 rpm for 10 min, the culture medium was discarded, and physiological saline was added for resuspending. 1 mL of bacterial solution was injected intraperitoneally.

[0065] The control group (C+TF group) of Scutellaria baicalensis leaves was fed with 1wt% Scutellaria baicalensis leaves ultrafine powder in the basal diet starting from the fourth day and continued to be fed until the end of the experiment. The rats were allowed to eat and drink freely during the experiment. On the 11th day of age, 1 mL of normal saline was injected intraperitoneally.

[0066] The RA+TF group was fed a basal diet supplemented with 1 wt% of safflower leaf powder from the fourth day until the end of the experiment. During the experiment, the animals were given free access to food and water. On the 11th day of age, the challenge was carried out at a concentration of 2.15×10 10 After bacterial recovery, the culture medium was centrifuged at 3500 rpm for 10 min, the culture medium was discarded, and physiological saline was added for resuspending. 1 mL of bacterial solution was injected intraperitoneally.

[0067] Body weight was measured before challenge (day 10), and defecation was observed 10 hours after challenge. The animals were sacrificed on days 1, 4, and 7 dpi for sampling. Eight animals were randomly selected for each sampling session and fasted for 12 hours before sacrifice. Body weight was measured, and the bacterial load in the target organs, heart, liver, and brain, of R. anatipestifer was determined.

[0068] 2. Test results 2.1 Changes in defecation like Figure 3 As shown, white diarrhea is white loose stools, and the yellow arrow represents white diarrhea. The defecation changes 10 hours after the infection. It can be seen from the figure that the Scutellaria serrata leaf group can significantly alleviate the white diarrhea caused by Riemerella anatipestifer infection.

[0069] 2.2 Weight changes like Figure 4 As shown in the results, the leaves of Scutellaria serrata can significantly increase body weight at 4dpi and 7dpi, and reduce the effect of weight loss caused by Riemerella anatipestifer infection, and the addition of Scutellaria serrata ultrafine powder to feed has no effect on the body weight of ducklings.

[0070] 2.3 Anatomical changes 1dpi liver anatomy diagram Figure 5 As shown, compared with group C ( Figure 5 Compared with A in the C+TF group ( Figure 5 There was no significant change in the RA group ( Figure 5 D) The liver was significantly enlarged, and the RA+TF group ( Figure 5 C) can alleviate liver swelling caused by RA infection. 1dpi heart anatomy diagram Figure 6 As shown, compared with group C ( Figure 6 Compared with A in the C+TF group ( Figure 6 There was no significant change in the RA group ( Figure 6 D) The heart showed obvious pericardial effusion, RA+TF group ( Figure 6 C) in the figure does not cause pericardial effusion.

[0071] 4dpi anatomical diagram Figure 7 As shown, compared with group C ( Figure 7 Compared with A in the C+TF group ( Figure 7There was no significant change in the RA group ( Figure 7 D) showed obvious fibrinous pericarditis and perihepatitis. Figure 7 C) No obvious fibrinous pericarditis or perihepatitis is present.

[0072] 7dpi anatomical diagram Figure 8 As shown, compared with group C ( Figure 8 Compared with A in the C+TF group ( Figure 8 There was no significant change in the RA group ( Figure 8 D) showed obvious fibrinous pericarditis and perihepatitis. Figure 8 C) No obvious fibrinous perihepatitis is present, but mild fibrinous pericarditis is present.

[0073] 2.4 Changes in bacterial load in target organs like Figure 9 As shown, cardiac bacterial load ( Figure 9 (A) Compared with the RA group, the bacterial load in the heart of the RA+TF group decreased significantly at 1 dpi, and very significantly at 4 and 7 dpi; the bacterial load in the liver ( Figure 9 B) Compared with the RA group, the bacterial load in the liver of the RA+TF group decreased significantly at 1 dpi and 4 dpi, and decreased extremely significantly at 7 dpi; the bacterial load in the brain tissue ( Figure 9 (C) Compared with the RA group, the bacterial load in brain tissue of the RA+TF group showed a significant decrease at 1 and 7 dpi, but a non-significant decrease at 4 dpi. This suggests that R. anatipestifer leaves can reduce the bacterial load in target organ tissues of ducklings infected with Riemerella anatipestifer.

[0074] Example 3 1. Materials and Methods 1.1 Experimental Animals A practical clinical trial was conducted from October 2024 to November 2024 at a Riemerella anatipestifer positive farm in Nanchang, Jiangxi Province.

[0075] 1.2. Investigational Drugs Ultrafine powder of Cyperus rotundus leaf.

[0076] 1.3 Test methods 800 one-day-old ducklings were randomly divided into group A and group B, with 400 ducks in each group. Group A did not receive any treatment (they were fed according to the normal feeding process of the farm), while group B was given 1wt% of Scutellaria baicalensis ultrafine powder in its feed starting from the 4th day of age and continued for 18 consecutive days.

[0077] The initial symptom of Riemerella anatipestifer is loose white feces. As the disease progresses, depression, neurological symptoms, and death may occur. During this period, the number of ducks with loose white feces, depression, neurological symptoms, deaths, and healthy ducks was recorded. Finally, 15 ducks were randomly selected from each group for autopsy and observation of anatomical lesions. White loose feces was defined as the presence of loose white matter in the feathers around the anus. Depression was defined as listlessness and huddling. Neurological symptoms were defined as head and neck tremors, a stargazing posture, and a reluctance to move. Healthy ducks were defined as those without any of the aforementioned symptoms, meaning they did not display the initial symptoms of the disease (loose white feces).

[0078] 2. Test results As shown in Table 2, the application of the Scutellaria serrata leaf preventive group in the positive farm of Riemerella anatipestifer can significantly reduce the discharge of white loose feces, mental depression, neurological symptoms, and mortality of ducks. Figure 11 As shown, the anatomy of group B is as follows Figure 10 As shown, the red circle represents fibrinous pericardium and the yellow circle represents fibrinous perihepatitis. Figure 10 As shown in the results, the ultrafine powder of Scutellaria baicalensis leaves can significantly alleviate the fibrinous pericarditis and perihepatitis caused by Riemerella anatipestifer.

[0079] Table 2 Effects of clinical application of ultrafine powder of Osmanthus fragrans leaves on ducklings infected with Riemerella anatipestifer

[0080] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. Application of the leaves of Rhizoma Citri Reticulatae in preparing products for preventing and / or treating Riemerella anatipestifer disease.

2. Application of round leaves of Scutellaria baicalensis in the preparation of products against Riemerella anatipestifer infection.

3. The use according to claim 1 or 2, characterized in that The Cyperus dasyphylla leaf is Cyperus dasyphylla leaf ultrafine powder, and the particle size of the Cyperus dasyphylla leaf ultrafine powder is less than 75 μm.

4. The use according to claim 2, characterized in that The anti-Riemerella anatipestifer infection includes any one of the following: 1) Alleviate any one or a combination of serositis, liver swelling and pericardial effusion in ducks caused by Riemerella anatipestifer infection; 2) Reduce mortality caused by Riemerella anatipestifer infection; 3) Reducing any one or a combination of white diarrhea, depression and neurological symptoms caused by Riemerella anatipestifer infection; 4) Reduce weight loss caused by Riemerella anatipestifer infection; 5) reducing any one or a combination of the bacterial load in the heart, liver, and brain tissue caused by Riemerella anatipestifer infection; Wherein, the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis.

5. A product for preventing and / or treating Riemerella anatipestifer disease, characterized in that: Including the round leaves of mountain fragrance.

6. A product for resisting Riemerella anatipestifer infection, characterized in that: Including the round leaves of mountain fragrance.

7. The product according to claim 5 or 6, characterized in that The Cyperus dasyphylla leaf is Cyperus dasyphylla leaf ultrafine powder, and the particle size of the Cyperus dasyphylla leaf ultrafine powder is less than 75 μm.

8. The product according to claim 6, characterized in that The anti-Riemerella anatipestifer infection includes any one of the following: 1) Alleviate any one or a combination of serositis, liver swelling and effusion in ducks caused by Riemerella anatipestifer infection; 2) Reduce mortality caused by Riemerella anatipestifer infection; 3) Reducing any one or a combination of white diarrhea, depression and neurological symptoms caused by Riemerella anatipestifer infection; 4) Reduce weight loss caused by Riemerella anatipestifer infection; 5) reducing any one or a combination of the bacterial load in the heart, liver, and brain tissue caused by Riemerella anatipestifer infection; Wherein, the serositis includes fibrinous pericarditis and / or fibrinous perihepatitis.

9. The product according to claim 5 or 6, characterized in that The dosage form of the product is an oral dosage form.

10. The product according to claim 9, characterized in that The oral dosage forms include oral liquid, granules, powders, powders, pills, tablets or capsules.

Images