Application of rice OsDREB6 and coding gene in regulating and controlling flumioxazin stress resistance of plants

Abstract

The invention relates to application of rice OsDREB6 and a coding gene in regulation and control of flumioxazin stress resistance of plants. The application comprises application of a rice transcription factor OsDREB6 and a coding gene thereof in regulation and control of flumioxazin stress resistance of plants and / or improvement of genetic breeding of flumioxazin tolerance of rice. Research finds that the rice transcription factor OsDREB6 participates in regulation and control of stress response of rice to flumioxazin for the first time, compared with wild type rice, rice seedlings overexpressed by OsDREB6 have the root length and bud length obviously larger than those of the wild type rice under the condition of flumioxazin stress, the rice seedlings show very good tolerance to flumioxazin, and the rice transcription factor OsDREB6 has good application prospects in regulation and control of the stress response of rice to flumioxazin. The damage of the flumioxazin to the rice is effectively reduced. Therefore, the rice transcription factor OsDREB6 can be used for improving the flumioxazin stress resistance of the rice and improving the molecular breeding of the flumioxazin resistance of the rice, and is of great significance to the cultivation of flumioxazin-resistant rice germplasm resources.

Description

Technical Field

[0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to application of rice OsDREB6 and encoding genes in regulating plant resistance to fluazifop-butyl stress. Background Art

[0002] Rice is a staple food for over half of the world's population, making it one of the world's most important crops. Weed damage has become a major obstacle to rice production. Currently, chemical herbicides, due to their economical and effective properties, are widely used on crops and have become the primary method for weed control. Based on the principle of "treating weeds early and at the smallest scale," soil sealing with herbicides is one of the most critical control measures.

[0003] Fluazifop-butyl is an N-phenylphthalimide herbicide and a photosynthesis inhibitor herbicide. It is mainly used as a soil sealer and can form a treatment layer on the soil surface. Once weeds germinate, they will come into contact with the treatment layer and die. Its mechanism of action is to inhibit the synthesis of protoporphyrinogen oxidase (PPO), leading to photosensitization and peroxidation of cell membrane lipids, causing irreversible damage to the function and structure of the cell membrane, thereby effectively preventing and controlling a variety of difficult-to-control malignant weeds with rapid effects and a long weed control period. However, while fluazifop-butyl exerts a highly effective weed control effect, it may also cause contact phytotoxicity to rice, affecting its normal growth and yield. In order to improve the simplicity and efficiency of weed control in rice fields, breeding fluazifop-butyl-resistant rice varieties has important practical significance for improving the effectiveness of early prevention and control of weeds in rice fields.

[0004] AP2 / ERF ethylene-responsive factors are one of the largest families of transcription factors in plants. They have been shown to directly regulate various plant developmental processes and respond to a variety of abiotic stresses, including drought, salinity, and cold. OsDREB6 is an AP2 / ERF transcription factor in rice. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to provide the use of rice OsDREB6 and its encoding gene in regulating plant resistance to fluazifop-butyl stress. By regulating the expression of the encoding gene of rice transcription factor OsDREB6 in plants, the tolerance of rice to fluazifop-butyl can be effectively improved.

[0006] The first aspect of the present invention is to provide the use of the gene encoding the rice transcription factor OsDREB6 in regulating plant resistance to fluazifop-butyl stress, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown in SEQ ID NO: 1.

[0007] The second aspect of the present invention is to provide the use of rice transcription factor OsDREB6 in regulating plant resistance to fluazifop-propyl stress. The amino acid sequence of the rice transcription factor OsDREB6 is shown in SEQ ID NO: 1.

[0008] The third aspect of the present invention is to provide the use of the gene encoding the rice transcription factor OsDREB6 in genetic breeding for improving plant tolerance to fluazifop-propyl. The nucleotide sequence of the encoding gene is shown in SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown in SEQ ID NO: 1.

[0009] The fourth aspect of the present invention is to provide the use of rice transcription factor OsDREB6 in genetic breeding for improving plant tolerance to fluazifop-propyl. The amino acid sequence of the rice transcription factor OsDREB6 is shown in SEQ ID NO: 1.

[0010] The fifth aspect of the present invention is to provide an overexpression vector inserted with a gene encoding rice transcription factor OsDREB6, wherein the nucleotide sequence of the encoding gene is shown in SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown in SEQ ID NO: 1.

[0011] The sixth aspect of the present invention is to provide the use of the overexpression vector as described above in genetic breeding for regulating plant resistance to fluazifop-propyl stress and / or improving plant tolerance to fluazifop-propyl.

[0012] In some embodiments, the plant comprises rice, sugarcane, or soybean, preferably rice.

[0013] The seventh aspect of the present invention is to provide a biological preparation for improving the tolerance of plants to fluazifop-propyl. The active ingredient of the biological preparation comprises the overexpression vector as described above.

[0014] The eighth aspect of the present invention is to provide a method for regulating plant resistance to fluazifop-butyl stress, the method comprising regulating the expression of a gene encoding a rice transcription factor OsDREB6 in a plant, wherein the nucleotide sequence of the encoding gene is as shown in SEQ ID NO: 2, or the nucleotide sequence encoding an amino acid sequence is as shown in SEQ ID NO: 1.

[0015] In some embodiments, the plant comprises rice, sugarcane, or soybean, preferably rice.

[0016] In some embodiments, the method for regulating the expression of the gene encoding the rice transcription factor OsDREB6 in plants includes Agrobacterium-mediated genetic transformation, gene gun method, CRISPR / dCas9 activation method, and chemical induction method.

[0017] The present invention, through research, has discovered for the first time that the rice transcription factor OsDREB6 is involved in regulating rice's stress response to the photosynthetic-inhibiting herbicide fluazifop-butyl. Compared with wild-type rice, rice seedlings overexpressing OsDREB6 showed no effect on root length under fluazifop-butyl stress conditions, and the effect on shoot length was significantly reduced, demonstrating excellent tolerance to fluazifop-butyl. This effectively reduced the damage caused by fluazifop-butyl to rice and promoted the growth and development of rice under fluazifop-butyl stress. Furthermore, overexpression of OsDREB6 did not affect the agronomic traits of rice. Therefore, the rice transcription factor OsDREB6 can improve rice's resistance to fluazifop-butyl and be used in molecular breeding of fluazifop-butyl-resistant rice, which is of great significance for cultivating fluazifop-butyl-resistant rice germplasm resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Figure 2 is a diagram of the OsDREB6 gene structure.

[0019] Figure 2 Protein characteristics and sequence analysis of OsDREB6 and homologous proteins in different species.

[0020] Figure 3 Cluster analysis of homologous proteins of OsDREB6 in different species.

[0021] Figure 4 To use fluorescence quantification to identify the relative expression level of OsDREB6 overexpressed rice genes.

[0022] Figure 5 The relative expression levels of OsDREB6 gene under flumioxazin stress at different time points after Huanghuazhan was sprayed with flumioxazin.

[0023] Figure 6 The tolerance of OsDREB6-overexpressing rice to fluazifop-butyl during seed germination.

[0024] Figure 7 Agronomic traits of OsDREB6-overexpressing rice and wild-type rice Huanghuazhan. DETAILED DESCRIPTION

[0025] To facilitate understanding of the present invention, the present invention will be described more fully below. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the present disclosure more thorough and comprehensive.

[0026] Experimental procedures in the following examples, where specific conditions are not specified, generally followed conventional conditions, such as those in Molecular Cloning: A Laboratory Manual (4th edition, edited by Green and Sambrook, published in 2013), or according to manufacturer recommendations. All commonly used chemical reagents used in the examples were commercially available.

[0027] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as those belonging to the art and

[0028] The terms "and / or" and "and / or" used in the present invention are intended to be used only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" as used in the present invention includes any and all combinations of one or more of the related listed items.

[0029] Furthermore, as used herein, the term "or" is inclusive and equivalent to the term "and / or," unless the context clearly dictates otherwise. The term "based on" is not exclusive and allows for being based on other factors not described, unless the context clearly dictates otherwise. Furthermore, throughout this specification, the meanings of "a," "an," and "the" include plural referents. The meaning of "in" includes "in" and "on."

[0030] The amino acid sequence of the rice transcription factor OsDREB6 of the present invention is shown in SEQ ID NO: 1.

[0031]

[0032] The nucleotide sequence of the gene encoding the rice transcription factor OsDREB6 is shown in SEQ ID NO: 2.

[0033]

[0034] The present invention is further described in detail below with reference to specific embodiments.

[0035] The Escherichia coli DH5α used in the following examples is a commonly used commercially available strain; the rice variety is Huanghuazhan, which is also commercially available. The primers used were synthesized by Shenzhen BGI Genomics Co., Ltd., and sequencing was performed at Shenzhen BGI Genomics Co., Ltd.

[0036] The quantitative tests in the following examples were all repeated three times, and the results were averaged.

[0037] Example 1 Construction of OsDREB6 gene overexpression vector

[0038] 1. Obtaining the Rice OsDREB6 Gene Fragment

[0039] The coding region of rice OsDREB6 gene (nucleotide sequence is shown in SEQ ID NO: 2, amino acid sequence is shown in SEQ ID NO: 1) was obtained from NCBI, and the gene structure diagram was drawn using GSDS2.0, as shown in Figure 1 The protein sequence is 291aa long and the nucleotide sequence is 876bp long. This gene has no introns and only one exon.

[0040] 2. Analysis and prediction of OsDREB6 homologous proteins

[0041] NCBI (https: / / www.ncbi.nlm.nih.gov / ) was used to search for homologous proteins of OsDREB6 in multiple species, and DNAMAN software was used to compare the sequences of homologous proteins of multiple species with OsDREB6. Figure 2 As shown in the figure, this protein is widely present in various plants, and the AP2 domain in its protein sequence is highly conserved. To further clarify the evolutionary relationship of OsDREB6, the amino acid sequences of DREB6 from species such as rice, Oryza sativa, Zizania latifolia, millet, Setaria viridis, Broomcorn millet, sorghum, maize, Phragmites australis, wheat, and Lolium multiflorum were selected and further analyzed for homology with OsDREB6 using MEGA11.0 software. Figure 3 As shown, OsDREB6 has a high homology with DREB6 of Oryza sativa, Oryza glaciosa, Wheat, and Lolium multiflorum, suggesting that DREB6 in these species have similar anti-fluazifop function.

[0042] 3. Construction of pCAMBIA1300-Ubi recombinant vector containing the above target gene

[0043] (1) Primer design and amplification

[0044] Adaptor primers were designed based on the OsDREB6 gene fragment, namely: F1: 5'-TGTTACTTGAGCTCGGTACCATGGCTGCAGCTATAGATCTGTCAGGGGAGGAGCT-3' (SEQ ID NO: 3); R1: 5'-AGGTCGACTCTAGAGGATCCATTGGCGGCGAGG AGGGAGTCCCAGTC-3' (SEQ ID NO: 4). The PCR reaction system is shown in Table 1.

[0045] Table 1 Target gene PCR reaction system

[0046]

[0047] The amplification procedure was as follows: 98°C for 3 minutes, 98°C for 10 seconds, 68°C for 1 minute, 32 cycles, and 68°C for 2 minutes. After completion, the electrophoresed fragment was excised and the target fragment was recovered using the Tiangen Biochemical Technology Co., Ltd. Agarose Gel DNA Recovery Kit (DP209-03) according to the kit instructions.

[0048] (2) Homologous recombination vector ligation and transformation

[0049] Ligation: The target fragment was homologously recombined with the linearized vector (pCAMBIA1300-Ubi) by seamless cloning with Gibson assembly mix. The homologous recombination ligation reaction system is shown in Table 2.

[0050] Table 2 Homologous recombination ligation reaction

[0051]

[0052] Transformation: (1) Take a tube of 100 μL DH5α E. coli competent cells and mix with 5 μL ligation product, and place on ice for 30 minutes; (2) Place in a 42°C constant temperature water bath, heat shock for 90 seconds, and place on ice for 2 minutes; (3) Add 500 μL LB liquid culture medium and mix well; (4) Incubate at 37°C and 200 rpm for 45 minutes to allow the cells to return to normal growth state; (5) Spread the bacterial solution evenly on a Kana-resistant LB solid culture medium plate; (6) After 30 minutes, place in a 37°C constant temperature incubator and culture overnight.

[0053] Colony PCR verification: Colony PCR verification was performed using primer pair F2: 5'-TTAGCCCTGCCTTCATACGC-3' (SEQ ID NO: 5); R2: 5'-ATCATCGCAAGACCGGCAAC-3' (SEQ ID NO: 6). The reaction system is shown in Table 3. The amplification program was as follows: 95°C for 5 min; 25 cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 2 min; and 72°C for 4 min.

[0054] Table 3 Colony PCR amplification system

[0055]

[0056] Plasmid extraction: (1) Pick a single colony with a positive PCR result from the LB solid culture medium and inoculate it into Kana-resistant LB liquid culture medium, and culture it at 37°C overnight; (2) Take 4 mL of activated bacterial solution, centrifuge it at 10,000 rpm at room temperature for 2 minutes, and completely discard the supernatant; (3) Take 250 μL of Solution I reagent containing ribonuclease A to thoroughly resuspend the bacterial block; (4) Take 250 μL of Solution II reagent to lyse the bacterial block, gently invert it upside down several times until the bacteria are transparent; (5) Take 350 μL of Solution III reagent, invert it several times until white compact flocs are formed; (6) Centrifuge it at 12,000 rpm at room temperature for 10 minutes, and take the supernatant; (7) Take out the nucleic acid purification column from the kit and place it on the collection tube; (8) Take the clarified supernatant from step 6 above to the nucleic acid purification column, centrifuge it at 12,000 rpm at room temperature for 1 minute, and discard the filtrate; (9) Take 500 μL of Buffer W1 was added to the nucleic acid purification column, centrifuged at 12000 rpm at room temperature for 30 seconds, and the filtrate was discarded; (10) 700 μL of Buffer W2 was added to the nucleic acid purification column, centrifuged at 12000 rpm at room temperature for 30 seconds, and the filtrate was discarded; (11) Repeat step 10; (12) The nucleic acid purification column was placed on the collection tube, and the column was centrifuged at 12000 rpm at room temperature for 2 minutes to remove the residual liquid as much as possible; (13) The collection tube was discarded, the nucleic acid purification column was placed in a 1.5 mL EP tube, and 50 μL of elution solution was added to elute the DNA attached to the nucleic acid purification column membrane (the elution solution can be preheated in a 65°C constant temperature water bath to help elute the DNA), and the column was allowed to stand at room temperature for 2 minutes; (14) The column was centrifuged at 12000 rpm at room temperature for 2 minutes to elute the DNA attached to the nucleic acid purification column membrane, and the column was stored in a -40°C low-temperature refrigerator for future use; (15) A trace amount of the recovered product was taken and the quality of the plasmid extraction was detected by agarose gel electrophoresis at a concentration of 1%. The overexpression vector was successfully constructed and verified by Sanger sequencing.

[0057] The positive clone plasmids were verified by PCR and sequencing. The sequencing results showed that the OsDREB6 gene fragment shown in SEQ ID NO: 2 was inserted between the two restriction enzyme sites of the vector pCAMBIA1300-Ubi. The obtained recombinant vector was named pCAMBIA1300-Ubi-OsDREB6.

[0058] 4. Rice genetic transformation

[0059] The pCAMBIA1300-Ubi-OsDREB6 recombinant vector was transformed into multiple Huanghuazhan rice calli using Agrobacterium-mediated method, and 7 OsDREB6-overexpressing rice strains were obtained.

[0060] Example 2 Screening and Verification of OsDREB6 Overexpressing Rice

[0061] The relative expression levels of the OsDREB6 gene in seven overexpressing rice lines were verified, using the wild-type variety Huanghuazhan as a control. RNA was extracted from aerial parts (stems and leaves) (OMEGA Cat#R6827-02) and reverse transcribed (Takara, PrimeScript RTreagent Kit with gDNA Eraser), followed by real-time quantitative PCR to detect OsDREB6 gene expression.

[0062] Real-time quantitative PCR was performed using primers F3: 5'-CAGGGGAGGAGCTGATGAGAG-3' (SEQ ID NO: 7); R3: 5'-GGCGAGGTGGGACTAAACT-3' (SEQ ID NO: 8). UBQ2 was used as an internal reference gene with the following primer sequences: F4: 5'-TGCTATGTACGTCGCCATCCAG-3' (SEQ ID NO: 9); R4: 5'-AATGAGTAACCACGCTCCGTCA-3' (SEQ ID NO: 10). The PCR reaction system (20 μL) was performed using SYBR Green Real-Time PCR Master Mix reagent (Takara) according to the product instructions. The specific system is shown in Table 4.

[0063] Table 4 Fluorescence quantitative PCR reaction system

[0064]

[0065] The amplification program was as follows: 95°C for 180 s; 95°C for 10 s, 60°C for 15 s, 40 cycles; 72°C for 30 s; 95°C for 10 s, 65°C for 60 s, and 97°C for 1 s.

[0066] The data were processed using the comparative Ct method, ΔCt = Ct(OsDREB6)-Ct(UBQ2), with 2 -ΔΔCt The value of was used to measure the gene transcription level, and the expression of OsDREB6 gene in the samples was analyzed and compared.

[0067] The results are as follows Figure 4 As shown, except for OsDREB6-1, 4, and 11 overexpressing rice, the expression level of OsDREB6 did not reach at least 1.5 times higher than that of wild-type rice Huanghuazhan, the other OsDREB6 overexpressing rice (OsDREB6-5, 7, 8, and 10 overexpressing rice) all showed significant upregulation of expression, indicating that these rices are OsDREB6 overexpression positive rice, and the OsDREB6-10 line with the highest OsDREB6 overexpression fold was selected for subsequent experiments.

[0068] Example 3 Real-time fluorescence quantitative test after treatment of rice with fluazifop-butyl

[0069] Preparation A (4g / hm 2 ) and B(97g / hm 2 Huanghuazhan plants, 25 days old and 4 leaves old, were sprayed with two solutions of flumioxazin (100 mg / ml) at two concentrations. Water was used as a control. Six hours after treatment, RNA was extracted from the aerial parts of the rice plants (OMEGACat #R6827-02) and reverse-transcribed into cDNA (Takara, PrimeScript RT reagent Kit with gDNAEraser). Real-time quantitative PCR was then performed to detect OsDREB6 gene expression. The real-time quantitative PCR method was as described in Example 2.

[0070] The results are as follows Figure 5 As shown in the figure, after 6 h of induction treatment with fluazifop-butyl at concentration B, the expression of OsDREB6 in the aboveground part of rice was upregulated compared with the control group treated with pure water, indicating that fluazifop-butyl can induce the expression of OsDREB6.

[0071] Example 4 Test on resistance of OsDREB6-overexpressing rice to fluazifop-butyl

[0072] The sensitivity of rice to fluazifop-butyl was observed by tissue culture seedlings. Prepare MS medium: Add 15g sucrose and 2.215g M&S basal medium with vitamins to 500mL of secondary water. Adjust the pH to 5.7 with NaOH, aliquot 80mL into each tissue culture flask, add 0.28g Phytagel, and sterilize with high-temperature steam. When the temperature drops to approximately 60°C, add fluazifop-butyl to a final concentration of 0.0023μM. Mix thoroughly, cool, and solidify before use. MS medium without fluazifop-butyl served as the untreated control. Seeds of the wild-type Huanghuazhan variety and the OsDREB6-overexpressing rice to be planted were revived at 49°C for 3-4 days. Before planting tissue culture seedlings, the seeds were disinfected: first, washed three times with 75% ethanol diluted in sterile water for 3 minutes each. Then, they were washed twice with a 30% sodium hypochlorite solution (with 1-2 drops of Tween 20 added to facilitate adhesion of the disinfectant to the seed surface), for 3 minutes and 20 minutes respectively. During washing, the seeds were shaken rapidly to remove impurities adhering to the surface, reducing the possibility of subsequent contamination. Finally, they were repeatedly washed with sterile water before being planted in the aforementioned MS medium. During the initial culture period (approximately 5 days), the seeds were incubated in the dark at 28°C. After emergence, the seeds were transferred to normal conditions (light / dark = 14 hours / 10 hours, 28°C) for another 10 days. Growth after the different treatments was photographed and recorded.

[0073] The results showed that under the condition of no pesticide application (no fluazifop-butyl added to MS medium), there was no significant difference in the growth of the aboveground and roots between OsDREB6-overexpressing rice (OsDREB6) and wild-type rice (WT); however, after the addition of fluazifop-butyl, the shoot length of OsDREB6-overexpressing seedlings was significantly less affected than that of the wild-type, and the root length of OsDREB6-overexpressing seedlings was not affected at all; while the wild-type seedlings showed obvious inhibition of aboveground and root growth ( Figure 6 The above results indicate that the OsDREB6 gene confers good tolerance to fluazifop-butyl in rice. Overexpression of the OsDREB6 gene can effectively enhance the resistance of rice to fluazifop-butyl and promote the growth of rice under fluazifop-butyl stress conditions.

[0074] Example 5 Agronomic traits of OsDREB6 overexpressing rice

[0075] Agronomic traits of wild-type Huanghuazhan and OsDREB6-overexpressing rice varieties in the Huanghuazhan background were investigated. Rice seedlings were cultured in a plant climate chamber and transplanted to a Hainan farm after reaching maturity (approximately 20-25 days). Topdressing or foliar fertilizer were regularly applied during the rice's fertilizer-demanding period. Plant height and panicle number were measured at harvest, and relevant phenotypic characteristics were photographed and recorded.

[0076] The results showed that the average plant height of the wild type and OsDREB6 overexpression were 97.5 cm and 98.6 cm, the average number of ears were 16 and 20, and the average ear length were 22.11 cm and 21.96 cm, respectively. Figure 7 Overexpression of OsDREB6 did not significantly affect the agronomic traits of Huang Huazhan, and OsDREB6-overexpressing rice had the ability to become a fluazifop-resistant rice variety.

[0077] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. Application of the gene encoding the rice transcription factor OsDREB6 in regulating plant resistance to fluazifop-propyl stress, characterized in that: The nucleotide sequence of the coding gene is shown as SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown as SEQ ID NO:

1.

2. Application of rice transcription factor OsDREB6 in regulating plant resistance to fluazifop-propyl stress, characterized in that: The amino acid sequence of the rice transcription factor OsDREB6 is shown in SEQ ID NO:

1.

3. Use of the gene encoding the rice transcription factor OsDREB6 in genetic breeding for improving plant tolerance to fluazifop-butyl, characterized in that: The nucleotide sequence of the coding gene is shown as SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown as SEQ ID NO:

1.

4. Application of rice transcription factor OsDREB6 in genetic breeding for improving plant tolerance to fluazifop-butyl, characterized in that: The amino acid sequence of the rice transcription factor OsDREB6 is shown in SEQ ID NO:

1.

5. An overexpression vector having an encoding gene for rice transcription factor OsDREB6 inserted therein, characterized in that: The nucleotide sequence of the coding gene is shown as SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown as SEQ ID NO:

1.

6. Use of the overexpression vector according to claim 5 in regulating plant resistance to fluazifop-propyl stress and / or improving plant tolerance to fluazifop-propyl in genetic breeding.

7. The use according to any one of claims 1 to 4 and 6, characterized in that: The plants include rice, sugarcane, and soybean, preferably rice.

8. A biological preparation for improving plant tolerance to fluazifop-butyl, characterized in that: The active ingredient of the biological preparation comprises the overexpression vector according to claim 5.

9. A method for regulating plant resistance to fluazifop-propyl stress, characterized in that: The method comprises regulating the expression of a gene encoding a rice transcription factor OsDREB6 in a plant, wherein the nucleotide sequence of the gene encoding the rice transcription factor OsDREB6 is shown in SEQ ID NO: 2, or the nucleotide sequence encoding the amino acid sequence is shown in SEQ ID NO:

1.

10. The method according to claim 9, wherein The plants include rice, sugarcane, soybean, preferably rice; and / or, The method for regulating the expression of the coding gene of the rice transcription factor OsDREB6 in plants includes Agrobacterium-mediated genetic transformation method, gene gun method, CRISPR / dCas9 activation method, and chemical induction method.

Images