Dairy cow and sheep nipple moisturizing and nourishing medicated bath liquid and preparation method thereof

Abstract

The invention relates to the field of animal winter skin care, and discloses a dairy cow and sheep nipple anti-freezing medicated bath liquid which comprises the following components in parts by mass: 50-60 parts of deionized water, 15-18 parts of a softening agent, 10-12 parts of an emollient, 8-12 parts of a humectant, 10-15 parts of an anti-freezing agent, 5-7 parts of essential oil, 4-5 parts of a traditional Chinese medicine extract, 3-5 parts of an antioxidant liquid and 5 parts of an antibacterial agent. The antifreezing agent is one of acidic phosphate amine salt, alkylamine, fatty acid amide, organic acid ester or alkyl succinimide, the antioxidant liquid is one of hindered phenol antioxidant, phosphite ester antioxidant or sulfur-containing antioxidant, and the antibacterial agent is povidone iodine. By adding the softening agent, the emollient and the humectant, the protective bath lotion can extend into the skin to improve the moistening degree of the skin, and meanwhile, the traditional Chinese medicine extract is added, so that the protective bath lotion has a very good immune regulation function, improves the resistance of an organism, nourishes dry and rough skin and further improves the anti-freezing effect.

Description

Technical Field

[0001] The invention relates to the technical field of animal skin in winter, and in particular to a moisturizing and curing medicinal bath for nipples of cows and sheep and a preparation method thereof. Background Art

[0002] Cows and sheep are common livestock in the agricultural industry that need to stay comfortable in cold winter conditions and avoid frostbite on their teats. Cow and sheep teat antifreeze dips are specialized products designed to prevent teat frostbite and are commonly used in the livestock industry. These dips help protect the teat skin from frostbite and damage caused by extreme cold weather.

[0003] Currently, most commercially available dairy cow and sheep nipple antifreeze agents are based on ethylene glycol or mineral oil systems, which rely on a single antifreeze ingredient to form a rigid protective layer. This type of formula is prone to crystallization or phase separation, which can cause the protective layer to rupture and fail to continuously lock in moisture. Repeated use may damage the skin's barrier function. In addition, existing products generally lack natural antibacterial ingredients, and long-term use can easily lead to an imbalance in the nipple surface flora or drug resistance problems. Although some technologies have attempted to introduce emulsifiers to improve stability, they have not solved the core defects of poor ingredient permeability and short moisturizing time at low temperatures. Traditional iodine preparations rely on complexing agents such as PVP, and long-term use can easily lead to a decrease in skin tolerance. The low-complexation sodium salt of dioctyl sulfosuccinate can avoid this risk. Therefore, there is an urgent need for a multi-effect medicinal bath that combines long-lasting antifreeze, deep moisturizing, natural antibacterial and skin repair functions. Summary of the Invention

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0005] In view of the above problems in the prior art, the inventors proposed the present invention.

[0006] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a dairy cow and sheep nipple moisturizing and curing medicinal dip and a preparation method thereof.

[0007] To solve the above technical problems, the present invention provides the following technical solution: a moisturizing and curing medicinal bath for the nipples of cows and sheep, comprising: the following components in parts by mass: 50-60 parts of deionized water, 15-18 parts of softener, 10-12 parts of emollient, 8-12 parts of moisturizer, 10-15 parts of antifreeze, 5-7 parts of essential oil, 4-5 parts of traditional Chinese medicine extract, 3-5 parts of antioxidant and 5 parts of antibacterial agent.

[0008] As a preferred embodiment of the present invention, the preparation method of the moisturizing and curing medicinal bath for the nipples of cows and sheep comprises the following steps:

[0009] S11: Weigh 15 parts of dioctyl adipate, 5 parts of dioctyl sebacate, 5 parts of carboxymethyl cellulose ester and 3 parts of compatibilizer in a mass ratio and put them into a mixing tank, slowly heat it to 60-70°C and stir to mix;

[0010] S12: Slowly cool the mixing tank to 15-20°C using a water bath, add 2 parts each of preservative and antioxidant, raise the temperature again to 45-55°C, and stir to mix;

[0011] S13: Slowly cool the mixing tank to room temperature using a water bath, add ammonia water to adjust the pH to 3-7, and obtain a softener.

[0012] As a preferred embodiment of the moisturizing and curing medicinal dip for nipples of cows and sheep described in the present invention, the compatibilizer in step S11 is prepared by mixing ethylene butyl acrylate and glycidyl methacrylate in a mass ratio of 1:2, the preservative in step S12 is potassium sorbate, and the antioxidant is a mixture of tea polyphenols, butylated hydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0013] As a preferred embodiment of the present invention, the moisturizing and curing medicinal bath for cow and sheep nipples, the preparation method of the emollient comprises the following steps:

[0014] S21: Weigh 5 parts of hydroxyethyl cellulose, 3 parts of glyceryl stearate, 8 parts of wetting agent, 2 parts of sustained-release agent, and 1 part of stabilizer by mass, place in a mixing tank, and heat to 80°C for 5-7 minutes while slowly stirring to mix;

[0015] S22: adding an antioxidant to a mixing tank, cooling the mixture to room temperature, and adding ammonia water to adjust the pH value to 5-6.5 to obtain an emollient.

[0016] As a preferred embodiment of the moisturizing and curing medicinal bath for dairy cow and sheep nipples described in the present invention, the wetting agent in step S21 is one of sodium lauryl sulfate, ethoxypolydimethylsiloxane or alkylphenol polyoxyethylene ether, the sustained-release agent is one or more of lecithin, sodium alginate, cyclomaltoheptaose, borax, vegetable oil and zinc sulfate heptahydrate, the stabilizer is isopropyl alcohol, and the antioxidant in step S22 is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0017] As a preferred embodiment of the present invention, the moisturizing and curing medicinal dip for dairy cow and sheep nipples, the preparation method of the moisturizing agent comprises the following steps:

[0018] S31: Weigh 15 parts of deionized water, 3 parts of loosening and water-retaining agent, 3 parts of hyaluronic acid, 2 parts of vitamin E, 1 part of urea capsule, and 1 part of emulsifier by mass, put them into a mixing tank, slowly heat to 60-65°C and continue stirring to mix thoroughly, and keep warm for 4-5 minutes;

[0019] S32: Add 2 parts of antioxidant into a mixing tank and stir to mix. Then, cool to room temperature and add preservative. After mixing again, add ammonia water to adjust the pH value to 3-7 to obtain a moisturizer.

[0020] As a preferred embodiment of the moisturizing and curing medicinal bath for nipples of cows and sheep described in the present invention, the loosening and water-retaining agent in step S31 is composed of grass fiber, peanut shells and zeolite, the mass ratio of the grass fiber, peanut shells and zeolite is 3:1:2, the emulsifier is one of polyoxyethylene ether, polyoxypropylene ether and sodium salt of dioctyl sulfosuccinate, the preservative in step S32 is potassium sorbate, and the antioxidant is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0021] As a preferred embodiment of the moisturizing and curing medicinal dip for the nipples of dairy cows and sheep described in the present invention, the antifreeze agent is one of acidic phosphate amine salts, alkylamines, fatty acid amides, organic acid esters or alkyl succinimide; the antioxidant liquid is one of hindered phenol antioxidants, phosphite antioxidants or sulfur-containing antioxidants; and the antibacterial agent is povidone iodine.

[0022] As a preferred embodiment of the present invention, the moisturizing and curing medicinal bath for dairy cow and sheep nipples comprises the following components: 18 parts of cloves, 54 parts of sophora flavescens, 60 parts of cnidium monnieri, 90 parts of wild chrysanthemum, 36 parts of mint, 36 parts of wolfsbane, 60 parts of lavender and 1.8 parts of borneol; and the preparation process comprises the following steps: adding the above materials to 3000 ml of petroleum ether, ultrasonically extracting at room temperature for 60 minutes, filtering, repeating the extraction twice, combining the filtrates, and then rotary evaporating the filtrate at 45° C. to a paste, dissolving the extract with methanol, transferring the extract to another container and continuing to spin-dry.

[0023] As a preferred embodiment of the method for preparing a moisturizing and curing medicinal dip for dairy cows and sheep nipples of the present invention, the method comprises the following preparation steps:

[0024] Step 1: Add deionized water, softener, emollient, moisturizer and antifreeze into a mixing tank, heat the mixing tank slowly to 75-85℃, stir thoroughly and keep warm for 3-5 minutes;

[0025] Step 2: Cool the mixing tank to 10-15°C, add antifreeze and essential oil, stir evenly, then heat up to 60-70°C again and keep warm for 5-8 minutes;

[0026] Step 3: Add the Chinese herbal medicine extract to the mixing tank, stir rapidly and continue to heat to 85-90°C, stir thoroughly and add the antioxidant solution, keep warm for 10-15 minutes to obtain a mixed solution;

[0027] Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

[0028] Beneficial effects of the present invention: The softener of the present invention promotes the penetration of the moisturizer by softening the stratum corneum, and the antifreeze forms a flexible protective film at low temperatures. The synergistic effect of the two significantly prolongs the moisturizing time and prevents the protective layer from falling off, thereby improving the antifreeze effect; the present invention increases the eugenol and matrine in the traditional Chinese medicine extract to respectively exert broad-spectrum antibacterial activity and skin immunomodulatory function, thereby reducing the risk of papillitis. It not only has a good immunomodulatory function and improves the body's resistance, but also has antioxidant and antibacterial functions, protects cells from bacterial and fungal infections, nourishes dry and rough skin, and further improves antifreeze. Effect: The compound formula of emollient and vitamin E promotes epidermal cell regeneration and repairs micro-damage to the skin caused by low temperature; the grass fiber water-retaining agent absorbs moisture through its porous structure, and combined with the staged heating process, ensures uniform dispersion of ingredients and enhances stability in low-temperature and humid environments; sodium salt of dioctyl sulfosuccinate is used as an emulsifier to enhance the low-temperature dispersibility of ingredients and maintain iodine dispersion stability at low temperatures, while avoiding damage to the skin barrier by traditional surfactants and enhancing the skin barrier repair function; allantoin promotes hydration of the stratum corneum and regeneration of epidermal cells, and synergizes with hyaluronic acid to accelerate the repair of frostbitten skin. DETAILED DESCRIPTION

[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0030] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0031] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it constitute a separate or selective embodiment that is mutually exclusive with other embodiments.

[0032] Example 1

[0033] This embodiment provides an antifreeze medicinal bath for the nipples of dairy cows and sheep, which includes the following components in parts by mass: 60 parts of deionized water, 18 parts of softener, 12 parts of emollient, 12 parts of moisturizer, 15 parts of antifreeze, 7 parts of essential oil, 5 parts of traditional Chinese medicine extract, 5 parts of antioxidant and 3 parts of antibacterial agent.

[0034] The preparation method of the softener comprises the following steps:

[0035] S11: Weigh 15 parts of dioctyl adipate, 5 parts of dioctyl sebacate, 5 parts of carboxymethyl cellulose ester and 3 parts of compatibilizer in a mass ratio and put them into a mixing tank, slowly heat it to 60-70°C and stir to mix;

[0036] S12: Slowly cool the mixing tank to 15-20°C using a water bath, add 2 parts each of preservative and antioxidant, raise the temperature again to 45-55°C, and stir to mix;

[0037] S13: Slowly cool the mixing tank to room temperature using a water bath, add ammonia water to adjust the pH to 3-7, and obtain a softener. It should be noted that the weakly acidic environment of pH 3-7 is consistent with the physiological characteristics of nipple skin, reduces irritation, and enhances the activity of the antibacterial agent.

[0038] Furthermore, in step S11, the compatibilizer is a mixture of ethylene butyl acrylate and glycidyl methacrylate in a mass ratio of 1:2, in step S12, the preservative is potassium sorbate, and the antioxidant is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0039] Furthermore, the preparation method of the emollient comprises the following steps:

[0040] S21: Weigh 5 parts of hydroxyethyl cellulose, 3 parts of glyceryl stearate, 8 parts of wetting agent, 2 parts of sustained-release agent, and 1 part of stabilizer by mass, place in a mixing tank, and heat to 80°C for 5-7 minutes while slowly stirring to mix;

[0041] S22: adding an antioxidant to a mixing tank, cooling the mixture to room temperature, and adding ammonia water to adjust the pH value to 5-6.5 to obtain an emollient.

[0042] Furthermore, in step S21, the wetting agent is one of sodium lauryl sulfate, ethoxypolydimethylsiloxane or alkylphenol polyoxyethylene ether, the sustained-release agent is one or more of lecithin, sodium alginate, cyclomaltoheptaose, borax, vegetable oil and zinc sulfate heptahydrate, the stabilizer is isopropyl alcohol, and the antioxidant in step S22 is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0043] Furthermore, the preparation method of the moisturizing agent comprises the following steps:

[0044] S31: Weigh 15 parts of deionized water, 3 parts of loosening and water-retaining agent, 3 parts of hyaluronic acid, 2 parts of vitamin E, 1 part of allantoin, and 1 part of emulsifier by mass, place them in a mixing tank, slowly heat to 60-65°C, and continue stirring to mix thoroughly, and keep warm for 4-5 minutes;

[0045] S32: Add 2 parts of antioxidant into a mixing tank and stir to mix. Then, cool to room temperature and add preservative. After mixing again, add ammonia water to adjust the pH value to 3-7 to obtain a moisturizer.

[0046] Furthermore, in step S31, the loosening and water-retaining agent is composed of grass fiber, peanut shells and zeolite, the mass ratio of grass fiber, peanut shells and zeolite is 3:1:2, the emulsifier is one of polyoxyethylene ether, polyoxypropylene ether, ethylene oxide and propylene oxide block copolymer, polyol fatty acid ester or polyvinyl alcohol, and the preservative in step S32 is potassium sorbate, and the antioxidant is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.5.

[0047] Furthermore, the antifreeze agent is one of acidic phosphate amine salt, alkylamine, fatty acid amide, organic acid ester or alkyl succinimide, the antioxidant liquid is one of hindered phenol antioxidant, phosphite antioxidant or sulfur-containing antioxidant, and the antibacterial agent is povidone iodine.

[0048] Furthermore, the components of the traditional Chinese medicine extract are 18 parts of cloves, 54 parts of sophora flavescens, 60 parts of cnidium monnieri, 90 parts of wild chrysanthemum, 36 parts of mint, 36 parts of wolfsbane, 60 parts of lavender and 1.8 parts of borneol. The preparation process is to add the above materials to 3000 ml of petroleum ether, perform ultrasonic extraction at room temperature for 60 minutes, and then filter, repeat twice, and combine the filtrate. Then, the filtrate is rotary evaporated at 45°C to a paste, dissolved with methanol, transferred to another container and continued to be spin-dried.

[0049] A method for preparing an antifreeze medicinal bath for the nipples of dairy cows and sheep comprises the following steps:

[0050] Step 1: Add deionized water, softener, emollient, moisturizer and antifreeze into a mixing tank, heat the mixing tank slowly to 85°C, stir thoroughly and keep warm for 5 minutes;

[0051] Step 2: Cool the mixing tank to 10-15°C, add antifreeze and essential oil, stir evenly, then heat up to 60°C again and keep warm for 5 minutes;

[0052] Step 3: Add the Chinese herbal medicine extract into the mixing tank, stir rapidly and continue to heat to 90°C, stir thoroughly and add the antioxidant solution, keep warm for 15 minutes, and obtain a mixed solution;

[0053] Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

[0054] Example 2

[0055] This embodiment provides an antifreeze medicinal bath for the nipples of dairy cows and sheep, which includes the following components in parts by mass: 55 parts of deionized water, 16 parts of softener, 11 parts of emollient, 10 parts of moisturizer, 12 parts of antifreeze, 6 parts of essential oil, 4.5 parts of traditional Chinese medicine extract, 4 parts of antioxidant liquid and 4 parts of antibacterial agent.

[0056] The preparation method of the softener is the same as that of Example 1, except that the compatibilizer in step S11 is replaced by ethylene butyl acrylate and methyl methacrylate mixed in a mass ratio of 1:1.

[0057] In the preparation method of the emollient, the wetting agent in step S21 is ethoxypolydimethylsiloxane, and the sustained-release agent is a mixture of sodium alginate and zinc sulfate heptahydrate in a mass ratio of 2:1.

[0058] In the preparation method of the moisturizing agent, the loosening and water-retaining agent in step S31 is adjusted to a mass ratio of grass fiber, peanut shell and zeolite of 2:1:1, and the emulsifier is polyol fatty acid ester.

[0059] The antifreeze agent is alkyl succinimide, and the antioxidant liquid is a phosphite antioxidant.

[0060] The composition of the Chinese medicine extract was adjusted to 20 parts of cloves, 50 parts of sophora flavescens, 65 parts of cnidium monnieri, 85 parts of wild chrysanthemum, 35 parts of mint, 35 parts of wolfsbane, 55 parts of lavender and 2 parts of borneol, and the extraction solvent was changed to ethanol.

[0061] The preparation steps are the same as those in Example 1, except that the temperature is raised to 80° C. in step 1 and the holding time in step 3 is 12 min.

[0062] Example 3

[0063] This embodiment provides an antifreeze medicinal bath for the nipples of dairy cows and sheep, which includes the following components in parts by mass: 58 parts of deionized water, 17 parts of softener, 10.5 parts of emollient, 11 parts of moisturizer, 14 parts of antifreeze, 6.5 parts of essential oil, 4.8 parts of traditional Chinese medicine extract, 4.5 parts of antioxidant liquid and 4.5 parts of antibacterial agent.

[0064] In the preparation method of the softener, the preservative in step S12 is replaced with sodium benzoate, and the antioxidant is adjusted to a mixture of tea polyphenols and tocopherols in a ratio of 3:1.

[0065] In the preparation method of the emollient, the stabilizer in step S21 is replaced with ethanol, and the sustained-release agent is a mixture of cyclomaltoheptaose and borax in a ratio of 1:1.

[0066] In the preparation method of the moisturizing agent, the loosening water-retaining agent in step S31 uses coconut shell fiber instead of grass fiber, and the mass ratio is coconut shell fiber: peanut shell: zeolite = 3:1:1.

[0067] The antifreeze agent is an organic acid ester, the antioxidant liquid is a sulfur-containing antioxidant, and the antibacterial agent is adjusted to chlorhexidine.

[0068] The content of honeysuckle was increased to 30 parts, the content of lavender was reduced to 50 parts in the composition of the traditional Chinese medicine extract, and the ultrasonic time in the extraction process was extended to 80 minutes.

[0069] In the preparation steps, step 2 is cooling to 12° C., and step 4 is adjusting the pH to 5-6.5.

[0070] Comparative Example 1

[0071] The invention discloses an antifreeze medicinal bath for the teats of dairy cows and sheep, which comprises the following components by mass: 50 parts of deionized water, 8 parts of moisturizer, 15 parts of antifreeze, 7 parts of essential oil, 4-5 parts of traditional Chinese medicine extract, 3 parts of antioxidant and 3 parts of antibacterial agent.

[0072] A method for preparing an antifreeze medicinal bath for the nipples of dairy cows and sheep comprises the following steps:

[0073] Step 1: Add deionized water, moisturizer and antifreeze into a mixing tank, heat the mixing tank slowly to 75°C, stir thoroughly and keep warm for 3 minutes;

[0074] Step 2: Cool the mixing tank to 10°C, add antifreeze and essential oil, stir evenly, then heat it up to 60°C again and keep it warm for 5 minutes;

[0075] Step 3: Add the Chinese herbal medicine extract into the mixing tank, stir rapidly and continue to heat to 90°C, stir thoroughly and add the antioxidant solution, keep warm for 10 minutes to obtain a mixed solution;

[0076] Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

[0077] Comparative Example 2

[0078] The invention discloses an antifreeze medicinal bath for the teats of dairy cows and sheep, comprising the following components in parts by mass: 50 parts of deionized water, 15 parts of a softener, 11 parts of an emollient, 12 parts of an antifreeze agent, 6 parts of essential oil, 4 parts of a traditional Chinese medicine extract, 3 parts of an antioxidant, and 3 parts of an antibacterial agent.

[0079] A method for preparing an antifreeze medicinal bath for the nipples of dairy cows and sheep comprises the following steps:

[0080] Step 1: Add deionized water, softener, emollient and antifreeze into a mixing tank, heat the mixing tank slowly to 85°C, stir thoroughly and keep warm for 5 minutes;

[0081] Step 2: Cool the mixing tank to 15°C, add antifreeze and essential oil, stir evenly, then heat it up to 70°C again and keep it warm for 8 minutes;

[0082] Step 3: Add the Chinese herbal medicine extract into the mixing tank, stir rapidly and continue to heat to 90°C, stir thoroughly and add the antioxidant solution, keep warm for 15 minutes, and obtain a mixed solution;

[0083] Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

[0084] Comparative Example 3

[0085] The invention discloses an antifreeze medicinal bath for the teats of dairy cows and sheep, comprising the following components in parts by mass: 50 parts of deionized water, 15 parts of a softener, 10 parts of an emollient, 8 parts of a moisturizer, 10 parts of an antifreeze agent, 5 parts of essential oil, 3 parts of an antioxidant, and 3 parts of an antibacterial agent.

[0086] A method for preparing an antifreeze medicinal bath for the nipples of dairy cows and sheep comprises the following steps:

[0087] Step 1: Add deionized water, softener, emollient, moisturizer and antifreeze into a mixing tank, heat the mixing tank slowly to 75°C, stir thoroughly and keep warm for 5 minutes;

[0088] Step 2: Cool the mixing tank to 15°C, add antifreeze and essential oil, stir evenly, then heat it up to 70°C again and keep it warm for 8 minutes;

[0089] Step 3: Add antioxidant liquid into the mixing tank and keep warm for 15 minutes to obtain a mixed solution;

[0090] Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

[0091] An equal number of female or male mice were selected as experimental subjects. The mice were fixed and their abdomens were extended into a temperature-controlled box. Conventional antifreeze on the market, the nipple antifreeze baths prepared in Examples 1, 2, and 3, and the nipple antifreeze baths prepared in Comparative Examples 1 to 3 were applied to the abdomens of the mice, respectively. The conventional antifreeze on the market served as the control group. The temperature of the temperature-controlled box was gradually lowered, and each temperature interval was maintained for 20 minutes. The changes in the abdomens of the mice were observed, and frostbite ratings were given. The following Table 1 is obtained.

[0092] No frostbite: No obvious changes in the abdomen, and the mice react normally;

[0093] First-degree frostbite: The skin is red, swollen, hot, and painful, but there is no obvious wart-like change or necrosis;

[0094] Second degree frostbite: superficial frostbite and deep frostbite, skin edema, pain, redness or purple;

[0095] Third degree frostbite: full-thickness frostbite and deep tissue frostbite, the skin becomes hard, cold, grayish white, and the mice lose sensation.

[0096] Table 1: Temperature (℃)

[0097]

[0098]

[0099] As can be seen from the above table, conventional antifreeze agents have good antifreeze effects when the temperature is above 0°C. However, when the temperature drops below 0°C, frostbite begins to appear on the abdomen of the mice, and as the temperature continues to drop, the frostbite level becomes higher and higher. However, the nipple antifreeze bath prepared in this embodiment maintains normal frostbite on the abdomen of the mice at -10 to -15°C.

[0100] Example 2 experienced first-degree frostbite at -15 to -10°C, possibly because the antifreeze (alkyl succinimide) was slightly less stable at low temperatures than the acidic phosphate amine salt in Example 1, and the ethanol extraction efficiency of the Chinese herbal medicine extract was relatively low, resulting in insufficient retention of the active ingredient. However, overall, the results were still significantly better than those of the comparative example.

[0101] Example 3 uses an organic acid ester antifreeze agent and a sulfur-containing antioxidant, combined with an improved traditional Chinese medicine extraction process (adding honeysuckle and extending the ultrasonic time), and still maintains no frostbite at extremely low temperatures (-15°C). Its antibacterial and moisturizing synergistic effects are better than those of Example 1.

[0102] In Comparative Example 1, the mice began to suffer from frostbite at -10 to -15°C. The reason was that no softener or emollient was added to the nipple antifreeze bath, so most of the liquid remained on the surface of the abdomen, the skin was poorly moisturized, and as the temperature dropped, the liquid began to harden, thereby losing its antifreeze effect.

[0103] The mice in Comparative Example 2 began to suffer from frostbite at -5 to -10°C. The reason was that no moisturizer was added to the nipple antifreeze bath. The moisturizer can prevent water loss, thereby ensuring the softness and smoothness of the skin, while softening the skin cuticle. The loss of moisturizer will accelerate water loss, resulting in antifreeze failure.

[0104] In Comparative Example 3, the mice began to suffer from frostbite at -5 to 0°C. The reason was that no Chinese herbal extract was added to the nipple antifreeze bath. The Chinese herbal extract is made from a variety of Chinese herbal ingredients, which not only has a good immune regulation function and improves the body's resistance, but also has antioxidant and antibacterial functions, protects cells from bacterial and fungal infections, and nourishes dry and rough skin.

[0105] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. An antifreeze medicinal bath for the nipples of dairy cows and sheep, characterized by: The composition includes the following components in parts by mass: 50-60 parts of deionized water, 15-18 parts of softener, 10-12 parts of emollient, 8-12 parts of moisturizer, 10-15 parts of antifreeze, 5-7 parts of essential oil, 4-5 parts of Chinese medicine extract, 3-5 parts of antioxidant and 5 parts of antibacterial agent.

2. The antifreeze medicinal bath for milk cow and sheep nipples according to claim 1, characterized in that: The preparation method of the softener comprises the following steps: S11: Weigh 15 parts of dioctyl adipate, 5 parts of dioctyl sebacate, 5 parts of carboxymethyl cellulose ester and 3 parts of compatibilizer in a mass ratio and put them into a mixing tank, slowly heat it to 60-70°C and stir to mix; S12: Slowly cool the mixing tank to 15-20°C using a water bath, add 2 parts each of preservative and antioxidant, raise the temperature again to 45-55°C, and stir to mix; S13: Slowly cool the mixing tank to room temperature using a water bath, add ammonia water to adjust the pH to 3-7, and obtain a softener.

3. The antifreeze medicinal bath for milk cow and sheep nipples according to claim 2, characterized in that: In step S11, the compatibilizer is prepared by mixing ethylene butyl acrylate and glycidyl methacrylate in a mass ratio of 1:

2. In step S12, the preservative is potassium sorbate. The antioxidant is a mixture of tea polyphenols, butylated hydroxytoluene and triphosphite in a mass ratio of 1:3:1.

5.

4. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 1, characterized in that: The preparation method of the emollient comprises the following steps: S21: Weigh 5 parts of hydroxyethyl cellulose, 3 parts of glyceryl stearate, 8 parts of wetting agent, 2 parts of sustained-release agent, and 1 part of stabilizer by mass, place in a mixing tank, and heat to 80°C for 5-7 minutes while slowly stirring to mix; S22: adding an antioxidant to a mixing tank, cooling the mixture to room temperature, and adding ammonia water to adjust the pH value to 5-6.5 to obtain an emollient.

5. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 4, characterized in that: In step S21, the wetting agent is one of sodium lauryl sulfate, ethoxypolydimethylsiloxane or alkylphenol polyoxyethylene ether, the sustained-release agent is one or more of lecithin, sodium alginate, cyclomaltoheptaose, borax, vegetable oil and zinc sulfate heptahydrate, the stabilizer is isopropyl alcohol, and the antioxidant in step S22 is a mixture of tea polyphenols, dibutylhydroxytoluene and triphosphite in a mass ratio of 1:3:1.

5.

6. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 1, characterized in that: The preparation method of the moisturizing agent comprises the following steps: S31: Weigh 15 parts of deionized water, 3 parts of loosening and water-retaining agent, 3 parts of hyaluronic acid, 2 parts of vitamin E, 1 part of allantoin, and 1 part of emulsifier by mass, place them in a mixing tank, slowly heat to 60-65°C, and continue stirring to mix thoroughly, and keep warm for 4-5 minutes; S32: Add 2 parts of antioxidant into a mixing tank and stir to mix. Then, cool to room temperature and add preservative. After mixing again, add ammonia water to adjust the pH value to 3-7 to obtain a moisturizer.

7. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 6, characterized in that: In step S31, the loosening and water-retaining agent is composed of grass fiber, peanut shells and zeolite, and the mass ratio of the grass fiber, peanut shells and zeolite is 3:1:2; The emulsifier is one of polyoxyethylene ether, polyoxypropylene ether, and sodium salt of dioctyl sulfosuccinate. The preservative in step S32 is potassium sorbate. The antioxidant is a mixture of tea polyphenols, dibutylhydroxytoluene, and triphosphite in a mass ratio of 1:3:1.

5.

8. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 1, characterized in that: The antifreeze agent is one of acidic phosphate amine salt, alkylamine, fatty acid amide, organic acid ester or alkyl succinimide, the antioxidant liquid is one of hindered phenol antioxidant, phosphite antioxidant or sulfur-containing antioxidant, and the antibacterial agent is povidone iodine.

9. The antifreeze medicinal bath for the nipples of dairy cows and sheep according to claim 1, characterized in that: The Chinese medicine extract comprises 18 parts of cloves, 54 parts of sophora flavescens, 60 parts of cnidium monnieri, 90 parts of wild chrysanthemum, 36 parts of mint, 36 parts of wolfsbane, 60 parts of lavender and 1.8 parts of borneol. The preparation process comprises the following steps: adding the above materials to 3000 ml of petroleum ether, performing ultrasonic extraction at room temperature for 60 minutes, and then filtering, repeating the extraction twice, combining the filtrates, and then rotary evaporating the filtrate at 45° C. to a paste, dissolving the extract with methanol, transferring the extract to another container, and continuing to spin-dry the extract.

10. A method for preparing the antifreeze medicinal bath solution for the nipples of dairy cows and sheep according to any one of claims 1 to 9, characterized in that: The method comprises the following preparation steps: Step 1: Add deionized water, softener, emollient, moisturizer and antifreeze into a mixing tank, heat the mixing tank slowly to 75-85℃, stir thoroughly and keep warm for 3-5 minutes; Step 2: Cool the mixing tank to 10-15°C, add antifreeze and essential oil, stir evenly, then heat up to 60-70°C again and keep warm for 5-8 minutes; Step 3: Add the Chinese herbal medicine extract to the mixing tank, stir rapidly and continue to heat to 85-90°C, stir thoroughly and add the antioxidant solution, keep warm for 10-15 minutes to obtain a mixed solution; Step 4: Cool the mixed solution to room temperature, add ammonia water into the mixing tank to adjust the pH value to 5-6.5, add antibacterial agent, and mix to obtain teat antifreeze bath solution.

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