Composite biological preparation for sewage treatment and preparation method thereof

Abstract

The present application belongs to the technical field of sewage treatment, and particularly relates to a composite biological agent for sewage treatment and a preparation method thereof. The composite biological agent comprises the following raw materials in parts by weight: modified bentonite 20-30 parts, unicellular algae 5-10 parts, Rhus succedanea leaf extract 5-10 parts, biological enzyme 1-3 parts, chitin 20-30 parts, trace elements 10-15 parts, and inorganic salt 10-20 parts. The preparation process of the modified bentonite is as follows: bentonite is added into a hexadecylmethyl dihydroxyethyl ammonium bromide aqueous solution and stirred, and then the pretreated bentonite is obtained by filtration and drying; the pretreated bentonite and ferric nitrate are added into water, and stirred to obtain an A solution; sodium carbonate and sodium phosphate are added into water, and stirred to obtain a B solution; the A solution is heated, and the B solution is added under stirring to react, and then the product is obtained by purification and calcination. The biological agent can improve the sewage treatment effect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of sewage treatment, and particularly relates to a composite biological agent for sewage treatment and a preparation method thereof. BACKGROUND

[0002] With the rapid development of industrial economy and cities, the amount of domestic sewage, kitchen wastewater and chemical wastewater in cities is increasing year by year. Investigations have found that sewage contains a large amount of organic matter and pollutants such as nitrogen and phosphorus, and even toxic and harmful substances. It can be seen that the composition of sewage is relatively complex, and if the sewage is directly discharged into groundwater, it will not only cause the water quality to deteriorate sharply, but also bring great negative effects to people's health and the sustainable development of society.

[0003] Sewage treatment methods include physical, chemical and biological methods, and the above-mentioned treatment methods can reduce the content of pollutants in sewage and achieve the purpose of improving water quality. The biological method is concerned by the sewage treatment field due to its low cost and easy practice. The principle of the biological method for treating sewage is to use microorganisms to degrade organic matter in sewage, remove nutrients such as ammonia nitrogen and total phosphorus in sewage, and improve the biodegradability of sewage.

[0004] At present, a variety of biological agents for sewage treatment have appeared on the market. However, it is found in practice that the existing biological agents for sewage treatment have the technical problem of poor sewage treatment effect. Therefore, it is urgent to provide a new biological agent for sewage treatment to accelerate the process of sewage treatment. SUMMARY

[0005] The first object of the present application is to provide a composite biological agent for sewage treatment, which can improve the sewage treatment effect.

[0006] The second object of the present application is to provide a preparation method of a composite biological agent for sewage treatment, which has simple preparation steps.

[0007] In order to achieve the above object, the technical solution adopted by the present application is as follows:

[0008] A composite biological agent for sewage treatment comprises the following raw materials by weight: modified bentonite 20-30 parts, unicellular algae 5-10 parts, Rhus succedanea leaf extract 5-10 parts, biological enzyme 1-3 parts, chitin 20-30 parts, trace elements 10-15 parts, and inorganic salt 10-20 parts.

[0009] The preparation process of the modified bentonite is as follows:

[0010] The bentonite is added to a hexadecylmethyl dihydroxyethyl ammonium bromide aqueous solution for stirring, and pretreated bentonite is prepared through filtration, washing and drying.

[0011] The pretreated bentonite and iron nitrate of step are added into water, and after being stirred uniformly, A solution is obtained; sodium carbonate and sodium phosphate are added into water, and after being stirred uniformly, B solution is obtained; the A solution is heated, and then B solution is added under stirring to react, and after the reaction is completed, the modified bentonite is prepared through purification and calcination.

[0012] Further, the solid-liquid ratio of the bentonite and the aqueous solution of cetyl methyl dihydroxyethyl ammonium bromide in step is 1g: (8-12)mL; and the concentration of the aqueous solution of cetyl methyl dihydroxyethyl ammonium bromide is 28-35g / L.

[0013] Further, the temperature of the stirring in step is 45-55℃, and the time of the stirring is 3-5h.

[0014] Further, in step , the mass ratio of the pretreated bentonite, iron nitrate and water in the A solution is (5-10):(6-12):(100-120); and the mass ratio of sodium carbonate, sodium phosphate and water in the B solution is (8-12):(4-8):(70-90).

[0015] Further, in step , the mass ratio of the pretreated bentonite in the A solution to sodium carbonate in the B solution is 1:(1.5-2.5).

[0016] Further, in step II, the temperature is raised to 75-85℃; the temperature of the reaction is 75-85℃, and the time of the reaction is 6-8h; and the temperature of the calcination is 800-1000℃, and the time of the calcination is 6-9h.

[0017] Further, the single-cell algae is photosynthetic green algae or nitrogen-fixing blue algae; and the biological enzyme is protease or amylase.

[0018] Further, the trace element is selected from one of manganese, cobalt, zinc and molybdenum; and the inorganic salt is composed of the following raw materials in weight parts: calcium chloride 10-15 parts, potassium dihydrogen phosphate 4-6 parts and magnesium sulfate 3-6 parts.

[0019] The preparation method of the composite biological preparation for sewage treatment comprises the following steps:

[0020] According to the weight part ratio of the raw materials, the raw materials are mixed uniformly, and then the composite biological preparation for sewage treatment is obtained.

[0021] Compared with the prior art, the beneficial effects of the present application mainly lie in that:

[0022] (1) The composite biological agent of the present application adds modified bentonite, Rhus succedanea leaf extract and other raw materials in the formula of the composite biological agent. Hexadecyl methyl dihydroxyethyl ammonium bromide is added in the bentonite to enhance the adsorption performance and dispersibility of the bentonite; then, iron nitrate, sodium carbonate and sodium phosphate are added in the pretreated bentonite to promote the change of the surface structure of the bentonite and form more pore channels, thereby improving the adsorption capacity thereof. The antioxidant components such as polyphenols in the Rhus succedanea leaf extract promote the growth and metabolism of microorganisms, thereby enhancing the treatment effect of the composite biological agent on sewage. The composite biological agent obtained by the present application is used for sewage treatment, and the results show that the use of the Rhus succedanea leaf extract and the modified bentonite can effectively improve the treatment effect of the sewage.

[0023] (2) After the sewage is treated by the composite biological agent of the present application for 3 days, the removal rate of COD in the sewage is 90.51% or more, the removal rate of ammonia nitrogen is 92.37% or more, and the removal rate of total phosphorus is 90.03% or more.

[0024] (3) The present application also provides a preparation method of the composite biological agent, and the preparation steps are simple and easy to practice. DETAILED DESCRIPTION

[0025] The technical solutions of the present application are further described below in combination with specific embodiments. However, those skilled in the art should understand that the following examples are only used to illustrate the present application, and should not be regarded as limiting the present application. The specific conditions not mentioned in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, such as those not specifically mentioned, are conventional products obtained through market channels.

[0026] The preparation method of the Rhus succedanea leaf extract is as follows: the Rhus succedanea leaves are dried and crushed to obtain Rhus succedanea leaf powder; the Rhus succedanea leaf powder is extracted with 65% ethanol aqueous solution at 60℃ for 2 hours according to the solid-liquid ratio of 1g:20mL, the extraction liquid is filtered and collected, and the crude extract is obtained by removing the solvent and drying; the crude extract is separated by using a macroporous resin, eluted with 65% ethanol aqueous solution, and the eluate is concentrated and dried to obtain the Rhus succedanea leaf extract.

[0027] Example 1

[0028] A composite biological agent for sewage treatment, the composite biological agent comprises the following raw materials in parts by weight: modified bentonite 25 parts, Chlorella 8 parts, Rhus succedanea leaf extract 6 parts, protease (200000U / g) 2 parts, chitin 22 parts, manganese 13 parts, inorganic salt 17 parts, wherein the inorganic salt is composed of the following raw materials in parts by weight: calcium chloride 12 parts, potassium dihydrogen phosphate 5 parts, magnesium sulfate 4 parts.

[0029] The preparation process of the modified bentonite is as follows:

[0030] The pretreated bentonite is prepared by adding bentonite to a 30 g / L aqueous solution of cetyl methyldihydroxyethyl ammonium bromide at a solid-liquid ratio of 1 g:10 mL, stirring at 50°C for 4 h, filtering, washing, and drying.

[0031] The pretreated bentonite of step is added to water, and stirred to obtain solution A. Sodium carbonate and sodium phosphate are added to water at a mass ratio of 10:6:80, and stirred to obtain solution B. Solution B is added dropwise to solution A at a mass ratio of pretreated bentonite to sodium carbonate of 1:2, and stirred at 80°C for 7 h. After the reaction is completed, the product is filtered, washed, dried, ground, and calcined at 900°C for 8 h to obtain modified bentonite.

[0032] The preparation method of the composite biological agent for sewage treatment is as follows:

[0033] The raw materials are mixed according to the weight ratio of each raw material, and the mixture is uniformly mixed.

[0034] Example 2

[0035] A composite biological agent for sewage treatment, the composite biological agent comprising the following raw materials by weight: 20 parts of modified bentonite, 5 parts of Synechococcus, 5 parts of Rhus succedanea leaf extract, 1 part of amylase (50000 U / g), 20 parts of chitin, 10 parts of zinc, and 10 parts of inorganic salt, wherein the inorganic salt is composed of the following raw materials by weight: 10 parts of calcium chloride, 4 parts of potassium dihydrogen phosphate, and 3 parts of magnesium sulfate.

[0036] The preparation process of the modified bentonite is as follows:

[0037] The pretreated bentonite is prepared by adding bentonite to a 28 g / L aqueous solution of cetyl methyldihydroxyethyl ammonium bromide at a solid-liquid ratio of 1 g:8 mL, stirring at 45°C for 5 h, filtering, washing, and drying.

[0038] The pretreated bentonite of step The pretreated bentonite, iron nitrate and water are added into water, stirred uniformly to obtain A solution; according to the mass ratio of sodium carbonate, sodium phosphate and water 8:4:70, sodium carbonate and sodium phosphate are added into water, stirred uniformly to obtain B solution; the A solution is heated to 75 DEG C, according to the mass ratio of pretreated bentonite in A solution and sodium carbonate in B solution 1:1.5, B solution is added into A solution under stirring, continuously reacts at 75 DEG C for 8h, after the reaction is completed, the modified bentonite is prepared through filtering, washing, drying, grinding and calcining at 800 DEG C for 9h.

[0039] The preparation method of the composite biological agent for sewage treatment is as follows:

[0040] According to the weight ratio of each raw material, the raw materials are mixed uniformly.

[0041] Example 3

[0042] A composite biological agent for sewage treatment, the composite biological agent comprises the following raw materials in parts by weight: modified bentonite 30 parts, Chlorella 10 parts, Rhus succedanea leaf extract 10 parts, protease (200000U / g) 3 parts, chitin 30 parts, molybdenum 15 parts, inorganic salt 20 parts, wherein the inorganic salt is composed of the following raw materials in parts by weight: calcium chloride 15 parts, potassium dihydrogen phosphate 6 parts, magnesium sulfate 6 parts.

[0043] The preparation process of the modified bentonite is as follows:

[0044] According to the solid-liquid ratio of bentonite and cetyl methyldihydroxyethyl ammonium bromide aqueous solution 1g:12mL, the bentonite is added into the cetyl methyldihydroxyethyl ammonium bromide aqueous solution with a concentration of 35g / L, stirred at 55 DEG C for 5h, and the pretreated bentonite is prepared through filtering, washing and drying;

[0045] According to the mass ratio of pretreated bentonite, iron nitrate and water 10:12:120, the pretreated bentonite and iron nitrate of step are added into water, stirred uniformly to obtain A solution; according to the mass ratio of sodium carbonate, sodium phosphate and water 12:8:90, sodium carbonate and sodium phosphate are added into water, stirred uniformly to obtain B solution; the A solution is heated to 85 DEG C, according to the mass ratio of pretreated bentonite in A solution and sodium carbonate in B solution 1:2.5, B solution is added into A solution under stirring, continuously reacts at 85 DEG C for 6h, after the reaction is completed, the modified bentonite is prepared through filtering, washing, drying, grinding and calcining at 1000 DEG C for 6h.

[0046] The preparation method of the composite biological agent for sewage treatment is as follows:

[0047] According to the weight ratio of each raw material, the raw materials are mixed uniformly.

[0048] Comparative Example 1

[0049] Comparative Example 1 differs from Example 1 in that no sumac leaf extract is added.

[0050] Comparative Example 2

[0051] Comparative Example 2 differs from Example 2 in that the modified bentonite is replaced by bentonite.

[0052] Performance detection

[0053] The sewage treatment effect of the preparations obtained in Examples 1-3 and Comparative Examples 1-2 is detected, and the detection process is as follows:

[0054] The preparations obtained in Examples 1-3 and Comparative Examples 1-2 are added to the sewage at a ratio of 1 g / 100 L, and the content of each index before sewage treatment is shown in Table 1. The content of COD, ammonia nitrogen and total phosphorus in the sewage is detected after 3 days, and the removal rate is calculated.

[0055] The detection method of each index is as follows:

[0056] COD: detected according to GB / T 11914-89 potassium dichromate method, and the results are shown in Table 2;

[0057] Ammonia nitrogen: detected according to HJ / T 195-2005 gas phase molecular absorption spectrum method, and the results are shown in Table 2;

[0058] Total phosphorus: detected according to GB 11893-89 ammonium molybdate spectrophotometric method, and the results are shown in Table 2.

[0059] Table 1: Content of each index before sewage treatment

[0060]

[0061] Table 2: Content of each index after sewage treatment and removal rate

[0062]

[0063] As shown in Table 2, the preparations obtained in Examples 1-3 have excellent sewage treatment effect. Compared with Example 1, Comparative Example 1 does not add sumac leaf extract, and Comparative Example 2 replaces the modified bentonite with bentonite, and the sewage treatment effect of the obtained preparations is reduced, which shows that the use of sumac leaf extract and modified bentonite can effectively improve the sewage treatment effect.

[0064] It can be known from the analysis that the addition of the cetyl methyldihydroxyethyl ammonium bromide enhances the adsorption performance and dispersibility of the bentonite, and the addition of the ferric nitrate, sodium carbonate and sodium phosphate promotes the change of the surface structure of the bentonite, more pore channels are formed, and the adsorption capacity is improved. In conclusion, the modified bentonite has better adsorption performance and higher adsorption capacity, and can remove the pollutants in the sewage more effectively. The antioxidant components such as polyphenols in the rhus succedanea leaf extract can promote the growth and metabolism of the microorganisms, and the treatment effect of the composite biological agent on the sewage is enhanced.

[0065] Finally, it should be pointed out that the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit it. The basic principles and main features of the present application have been described above with specific embodiments, and some modifications or replacements can be made on the basis of the present application, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the present application.

Claims

1. A compound biological agent for wastewater treatment, characterized in that, The raw materials include the following parts by weight: 20-30 parts modified bentonite, 5-10 parts single-celled algae, 5-10 parts sumac leaf extract, 1-3 parts bio-enzyme, 20-30 parts chitin, 10-15 parts trace elements, and 10-20 parts inorganic salts. The preparation process of the modified bentonite is as follows: Bentonite was added to an aqueous solution of hexadecylmethyldihydroxyethylammonium bromide and stirred. After filtration, washing, and drying, pretreated bentonite was obtained. Steps Pretreated bentonite and ferric nitrate were added to water and stirred until homogeneous to obtain solution A; sodium carbonate and sodium phosphate were added to water and stirred until homogeneous to obtain solution B; solution A was heated and solution B was added under stirring to react; after the reaction was completed, the solution was purified and calcined to obtain modified bentonite. step The solid-liquid ratio of the bentonite and hexadecylmethyldihydroxyethylammonium bromide aqueous solution is 1 g: (8-12) mL; the concentration of the hexadecylmethyldihydroxyethylammonium bromide aqueous solution is 28-35 g / L. step The mass ratio of pretreated bentonite, ferric nitrate, and water in solution A is (5-10):(6-12):(100-120); the mass ratio of sodium carbonate, sodium phosphate, and water in solution B is (8-12):(4-8):(70-90). step The mass ratio of pretreated bentonite in solution A to sodium carbonate in solution B is 1:(1.5-2.5). step The calcination temperature described herein is 800-1000℃, and the calcination time is 6-9 hours.

2. The composite biological agent for wastewater treatment as described in claim 1, characterized in that, step The stirring temperature is 45-55℃ and the stirring time is 3-5 hours.

3. The composite biological agent for wastewater treatment as described in claim 1, characterized in that, step The temperature is raised to 75-85℃; the reaction temperature is 75-85℃, and the reaction time is 6-8h.

4. The composite biological agent for wastewater treatment as described in claim 1, characterized in that, The single-celled algae are photosynthetic green algae or nitrogen-fixing cyanobacteria; the biological enzymes are proteases or amylases.

5. The composite biological agent for wastewater treatment as described in claim 1, characterized in that, The trace element is selected from one of manganese, cobalt, zinc, and molybdenum; the inorganic salt is composed of the following raw materials in parts by weight: 10-15 parts calcium chloride, 4-6 parts potassium dihydrogen phosphate, and 3-6 parts magnesium sulfate.

6. The method for preparing the composite biological agent for wastewater treatment according to any one of claims 1-5, characterized in that, Includes the following steps: Mix the raw materials thoroughly according to their respective weight proportions.

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