Nucleic acid error suppression
By using Ultima Genomics' low-cost, high-throughput sequencing platform and deep WGS technology, combined with UMI error suppression, the problems of high sequencing error rate and high cost of ctDNA detection in low-load disease settings were solved, achieving a sequencing error rate as low as 2.7×10-7, which is suitable for accurate cancer monitoring of clinical samples.
CN120813690APending Publication Date: 2025-10-17CORNELL UNIVERSITY
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Patent Information
- Application Number
- CN202380088311.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2022-10-25
- Filing Date
- 2023-10-25
- Publication Date
- 2025-10-17
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Figure CN120813690A_ABST
Abstract
Nucleic acid error inhibition is provided. In various embodiments, DNA is extracted from a collection of plasma samples. A sequence library having a duplex adaptor is prepared. The library is prepared by linking a duplex adaptor having a unique molecular identifier (UMI) to the end of each of a plurality of strands of the extracted DNA and amplifying the extracted DNA with a first polymerase chain reaction (PCR). A subset of the whole genome library is selected and amplified with a second PCR to increase the amount of PCR replicas. A plurality of duplex reads are sequenced from the amplified subset.
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