Lysozyme composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a lysozyme composition as well as a preparation method and application thereof, and the IPC classification number is A61K8. The lysozyme composition is used in cosmetics, can dissolve and adsorb fat and dirt on the skin surface, removes aged cutin of the skin, and achieves the effects of cleaning and smoothing the skin; generation of skin collagen and elastic fibers is promoted, so that the skin is more compact and is rich in elasticity, and the skin tendering effect is achieved; generation of melanin can be inhibited, catabolism of melanin is promoted, and the skin brightening effect is achieved; the acne-removing mask has a bacteriostatic effect, and achieves an acne-removing effect by inhibiting inflammatory response of skin and regulating grease secretion of the skin; meanwhile, the effect of relieving the skin is achieved, and the discomfort of skin tension can be relieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cleaning cosmetics, in particular to a lysozyme composition and a preparation method and application thereof. BACKGROUND

[0002] In today's era of skin care, cleaning mud masks have become an indispensable category in the cosmetics market due to their unique effects. Consumers' pursuit of skin health and beauty continues to rise, and cleaning, as the foundation of skin care, is becoming increasingly important. Cleaning mud masks can deeply penetrate into skin pores, effectively adsorb and remove impurities such as dirt and excess oil on the skin surface, laying a good foundation for subsequent skin care steps, allowing the skin to better absorb nutrients, and thus are favored by consumers.

[0003] The ingredients in the application face mask have strong adsorption, making it have strong cleaning effect. When adsorbing dirt and oil, it will take away the moisture and natural moisturizing factors on the skin surface, causing the skin to appear dry and tight after cleaning. Long-term use may damage the skin barrier, making the skin sensitive and fragile. Although the application face mask has strong cleaning effect, it has limited ability to inhibit bacteria such as Propionibacterium acnes, and the exfoliation function is single and rough, with little effect on brightening skin color, and cannot effectively improve skin dullness and yellowing. At the same time, for the problems of large pores and oily skin caused by excessive oil secretion, the existing application face mask lacks targeted fat-dissolving mechanism and cannot fundamentally reduce skin oil secretion and maintain skin freshness.

[0004] With people's increasing attention to skin health and appearance, consumers' demand for application face masks is becoming increasingly diverse. In addition to basic cleaning functions, consumers expect mud masks to have multiple functions such as bacteria inhibition, acne removal, exfoliation, skin smoothing, skin tendering, brightening, and fat-dissolving, to achieve one-stop skin care and meet the pursuit of efficient skin care in modern fast-paced life. Therefore, it is of great market value and practical significance to develop a composition that can overcome the shortcomings of existing application face masks and integrate bacteria inhibition, acne removal, exfoliation, skin smoothing, skin tendering, brightening, and fat-dissolving into one, which is expected to bring consumers a better and more comprehensive skin care experience. SUMMARY

[0005] The present application aims to provide a lysozyme composition and a preparation method and application thereof. The lysozyme composition provided by the present application is used in the application face mask, which can improve the tightness of using the existing application face mask, and has the effects of bacteria inhibition, acne removal, exfoliation, skin smoothing, skin tendering, brightening, and fat-dissolving.

[0006] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions: The present application provides a lysozyme composition, which comprises the following raw materials in parts by weight: 0.05-0.5 parts of lysozyme, 0.05-0.3 parts of surfactant, 0.05-0.2 parts of superoxide dismutase, 0.1-1 parts of malve extract, 0.5-1.5 parts of lotus extract, 0.5-1 parts of peach blossom extract, 0.5-1.2 parts of magnolia extract and 0.5-2 parts of licorice extract.

[0007] The present application also provides a preparation method of the lysozyme composition, which comprises mixing the lysozyme, the surfactant, the superoxide dismutase, the malve extract, the lotus extract, the peach blossom extract, the magnolia extract and the licorice extract.

[0008] The present application also provides an application of the lysozyme composition in preparing cosmetics (including but not limited to facial mask, lotion, cream, etc.).

[0009] Preferably, the cleaning cosmetic comprises a facial mask.

[0010] The present application also provides a facial mask containing the lysozyme composition, which comprises the following raw materials in parts by weight: 4-6 parts of the lysozyme composition, 35-45 parts of an adsorbent, 2-2.5 parts of a skin conditioning agent, 6-7 parts of a skin feel modifier, 4-6 parts of a humectant, 3-5 parts of an emulsifier, 0.5-1.5 parts of a preservative and 30-45 parts of deionized water.

[0011] Preferably, the adsorbent is obtained by high-temperature treatment of kaolin, montmorillonite and diatomite.

[0012] More preferably, the preparation method of the adsorbent comprises: separately heating the kaolin, the montmorillonite and the diatomite at 100-200℃ for 30-60 min, crushing, sieving through a 300-500 mesh sieve, and mixing according to the weight ratio (15-23):(8-13):(8-13) to obtain the adsorbent.

[0013] Preferably, the skin conditioning agent is one or more of panthenol, ceramide 3 and ursolic acid; and the skin feel modifier is one or more of squalane, ethylhexyl palmitate and cetyl PEG / PPG-10 / 1 dimethicone.

[0014] Preferably, the humectant is one or more of hydrolyzed sodium hyaluronate, 2-methyl-2,4-pentanediol and 1,3-butanediol; the emulsifier is one or more of sorbitan stearate, cetyl stearyl alcohol and PEG-20 stearate; and the preservative is one or more of phenoxyethanol, sodium benzoate and chlorphenesin.

[0015] The application further provides a preparation method of the above-mentioned smearing mask, comprising: mixing deionized water and a humectant to obtain a mixture, melting an emulsifier at 60-80 DEG C and then cooling it to 35-45 DEG C, and mixing the emulsifier, the mixture, an adsorbent, a lysozyme composition, a skin conditioner and a preservative to obtain the smearing mask.

[0016] Compared with the prior art, the application has the following beneficial effects: The application provides a lysozyme composition, comprising: lysozyme, superoxide dismutase, shu tian enzyme, hibiscus mutabilis flower extract, lotus flower extract, peach flower extract, magnolia kobus extract and glycyrrhiza glabra extract. The lysozyme composition is used in the smearing mask, can dissolve and adsorb the fat and dirt on the surface of the skin, remove the aged cutin of the skin, achieve the effect of cleaning and smoothing the skin, promote the generation of collagen and elastic fiber of the skin, make the skin more compact and elastic, achieve the effect of tendering the skin, can inhibit the generation and promote the decomposition and metabolism of melanin, achieve the effect of lightening the skin, have the effect of inhibiting bacteria, can achieve the effect of removing acne by inhibiting the inflammatory reaction of the skin and regulating the secretion of skin oil, and have the effect of soothing the skin, can relieve the tight and uncomfortable feeling of the skin, and the adsorbent in the smearing mask can be heated to relieve the poor skin feeling and the dry and tight feeling after using the mud mask. The components in the mud mask have synergistic effects, can achieve the multiple effects of inhibiting bacteria, removing acne, removing cutin, smoothing the skin, tendering the skin, lightening and dissolving fat. DETAILED DESCRIPTION

[0017] The application provides a lysozyme composition, comprising the following raw materials in parts by weight: 0.05-0.5 parts of lysozyme, 0.05-0.3 parts of superoxide dismutase, 0.05-0.2 parts of shu tian enzyme, 0.1-1 parts of hibiscus mutabilis flower extract, 0.5-1.5 parts of lotus flower extract, 0.5-1 parts of peach flower extract, 0.5-1.2 parts of magnolia kobus extract and 0.5-2 parts of glycyrrhiza glabra extract.

[0018] The lysozyme composition has synergistic effects, can achieve the multiple effects of inhibiting bacteria, removing acne, removing cutin, smoothing the skin, tendering the skin, lightening and dissolving fat. In terms of inhibiting bacteria, the lysozyme as a direct bacteriostatic component can inhibit the growth of various bacteria, create a healthy microbial environment for the skin, the glycyrrhiza glabra extract has the inhibiting effect on common harmful bacteria such as propionibacterium acnes, and has the common effect with the lysozyme, expands the bacteriostatic spectrum and enhances the bacteriostatic effect, and the hibiscus mutabilis flower extract assists the lysozyme and the glycyrrhiza glabra extract to further inhibit the bacterial reproduction, the three components synergistically maintain the balance of the skin microecology and reduce various skin problems caused by bacterial infection.

[0019] In terms of acne, lysozyme can inhibit acne-causing bacteria such as Propionibacterium acnes and reduce inflammation, thereby reducing the occurrence of acne from the root. It also improves the microenvironment around acne and helps to heal acne. Glycyrrhiza glabra extract not only inhibits bacteria but also inhibits the release of inflammatory factors through its anti-inflammatory effect, thereby relieving symptoms such as redness and pain caused by acne. It works with lysozyme to combat acne inflammation. Lotus extract has heat-clearing and detoxifying properties, which, together with the previous two ingredients, improve the condition of acne-prone skin and accelerate the regression of acne.

[0020] In terms of exfoliation, subtilisin can specifically recognize and hydrolyze the proteins that form the intercellular junctions (mainly keratin envelope proteins), promoting the shedding of aged keratin layer. Peach extract regulates keratinocyte metabolism and assists subtilisin to make the exfoliation process more gentle and uniform, avoiding damage to the skin caused by excessive exfoliation. Together, they promote keratin layer metabolism.

[0021] In terms of smoothing the skin, subtilisin can improve the unevenness of the skin surface after removing aged keratin, making the skin smooth. It also promotes skin metabolism, allowing newly born keratinocytes to arrange more neatly and enhancing the smoothness of the skin. Hibiscus mutabilis extract hydrates the skin, fills the skin cells, reduces the roughness caused by dryness, and improves skin texture. Together with subtilisin, it makes the skin feel smoother. Magnolia kobus extract can reduce free radical damage and maintain skin elasticity, making the skin surface smooth and delicate. Together with the previous two ingredients, it improves the smoothness of the skin in all directions.

[0022] In terms of skin rejuvenation, superoxide dismutase efficiently eliminates free radicals, reduces cell damage, prevents skin aging, promotes cell metabolism and repair of damaged cells, and keeps the skin young and delicate. Hibiscus mutabilis extract, lotus extract, peach extract, and magnolia kobus extract are rich in nutrients that provide nourishment to the skin, enhance skin vitality, and keep the skin moist and full, improving skin texture. Together with superoxide dismutase, they achieve the effect of skin rejuvenation. Glycyrrhiza glabra extract improves the overall health of the skin through whitening, anti-inflammatory, and antioxidant effects, making the skin tight and shiny. Together with other ingredients, it enhances the delicacy of the skin.

[0023] In terms of brightening, glycyrrhiza glabra extract can strongly inhibit tyrosinase activity and reduce melanin production, making it a key ingredient for brightening the skin. Peach extract can inhibit melanin production and reduce free radical damage to the skin through antioxidant effects, improving dullness. Together with glycyrrhiza glabra extract, it brightens the skin. Lotus extract can eliminate free radicals, reduce the impact of oxidative stress on the skin, and restore the natural glow of the skin. Together with the previous two ingredients, it enhances the brightening effect and makes the skin whiter and more transparent.

[0024] In terms of fat-dissolving, lysozyme decomposes skin surface oil, cleanses oil accumulation in pores, improves pore blockage caused by excessive oil secretion, and assists mud mask in cleaning oil; glycyrrhiza glabra extract regulates skin metabolic function, reduces excessive oil secretion, cooperates with lysozyme, controls skin oil content, and keeps skin clean and fresh.

[0025] The application further provides a preparation method of the lysozyme composition, comprising: mixing lysozyme, lysozyme, superoxide dismutase, malve extract, lotus extract, peach blossom extract, magnolia extract and glycyrrhiza glabra extract.

[0026] The application further provides application of the lysozyme composition in preparation of a cleaning cosmetic, and the cleaning cosmetic comprises a mask.

[0027] The lysozyme composition can also be preferably used in water, emulsion, essence, cream, and bath cosmetic.

[0028] The application further provides a mask containing the lysozyme composition, comprising the following raw materials in parts by weight: 4-6 parts of lysozyme composition, 35-45 parts of adsorbent, 2-2.5 parts of skin conditioner, 6-7 parts of skin feel regulator, 4-6 parts of humectant, 3-5 parts of emulsifier, 0.5-1.5 parts of preservative, and 30-45 parts of deionized water.

[0029] The adsorbent is preferably obtained by high-temperature treatment of kaolin, montmorillonite and diatomite; and the preparation method of the adsorbent preferably comprises: respectively heating kaolin, montmorillonite and diatomite at 100-200℃ for 30-60min, crushing, sieving through a 300-500 mesh sieve, and mixing according to a weight ratio of (15-23):(8-13):(8-13) to obtain the adsorbent.

[0030] The heating treatment of the adsorbent changes its surface charge, pore structure, shape and hardness, thereby forming more active sites and having stronger adsorption capacity to bacteria, deeply penetrating into pores, adsorbing toxins such as excessive oil, dandruff and bacterial metabolites generated by acne, reducing clogging of hair follicles, and reducing the probability of acne generation from the root, helping to regulate the skin microenvironment, reduce inflammation, relieve acne inflammation symptoms, and promote the healing of acne. In the process of applying and washing the mask, the adsorbent contacts the skin surface, enhances its physical friction effect on the aged stratum corneum of the skin, helps to remove the accumulated waste keratinocytes on the skin surface, stimulates and promotes the metabolism of the skin, accelerates the renewal rate of skin cells, improves the overall flatness of the skin, enhances the smoothness and softness of the skin, and makes the skin more delicate. After removing the aged keratinocytes and oil, the light transmittance of the skin is enhanced, and the reflection of light on the skin surface is more uniform, making the skin look brighter and more lustrous. Crushing the adsorbent not only makes it have good adsorption, but also makes it have delicate texture.

[0031] The skin conditioning agent is preferably one or more of panthenol, ceramide 3, and ursolic acid; the skin feel modifier is preferably one or more of squalane, ethylhexyl palmitate, and cetyl PEG / PPG-10 / 1 dimethicone; the humectant is preferably one or more of sodium hyaluronate, 2-methyl-2,4-pentanediol, 1,3-butanediol, and urea; the emulsifier is preferably one or more of sorbitan stearate, cetyl stearyl alcohol, and PEG-20 stearate; and the preservative is preferably one or more of phenoxyethanol, sodium benzoate, and chlorphenesin.

[0032] The application further provides a preparation method of the above-mentioned application mask, comprising: mixing deionized water and a humectant to obtain a mixture, melting the emulsifier at 60-80°C and then cooling it to 35-45°C, and mixing the emulsifier with the mixture, the adsorbent, the lysozyme composition, the skin conditioning agent and the preservative to obtain the application mask.

[0033] The use method of the application mask is as follows: after cleansing, take an appropriate amount of the application mask and apply it to the face (avoiding the eyes and the area around the lips), evenly spread it, make the thickness of 1-2 mm, stand for 10-15 min, and then wash it off with clean water, use it before going to bed every day, and continuously use it for 4 weeks.

[0034] In the application, all raw material components are commercially available products well known to those skilled in the art unless otherwise specified.

[0035] The technical solutions in the present application will be described clearly and completely below in connection with the embodiments in the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative effort belong to the protection scope of the present application.

[0036] In the following examples, unless otherwise specified, all are conventional methods.

[0037] In the following examples, unless otherwise specified, all the materials, reagents, etc. can be obtained from commercial channels.

[0038] In the following examples and comparative examples, lysozyme and superoxide dismutase were purchased from Guangzhou Dr. Papi Biological Technology Co., Ltd., the enzyme activity of lysozyme was 500 billion IU, and the enzyme activity of superoxide dismutase was 500 billion IU; surufatin was purchased from Shanghai Yu Yu Biological Technology Co., Ltd., with a specification of 4000 u / mg; hibiscus sabdariffa flower extract was purchased from Shanghai Yuyu New Material Technology Co., Ltd., lotus flower extract was purchased from Lanzhou Waterless Biological Technology Co., Ltd., peach flower extract was purchased from Shaanxi Greenley Biological Technology Co., Ltd., magnolia kobus extract was purchased from Wuhan Greenfield Biological Technology Co., Ltd., licorice extract was purchased from Sichuan Huanxi Biological Technology Co., Ltd., cetyl PEG / PPG-10 / 1, and dimethicone were purchased from Wuhan Lanya Bai Pharmaceutical Chemical Co., Ltd., kaolin was purchased from Xinzhou Xin Fu District Xinzhe Kaolin Building Material Co., Ltd., montmorillonite was purchased from Wuhu Shuohua New Material Technology Co., Ltd., and diatomite was purchased from Lingshou Zhengdong Mineral Products Co., Ltd.

[0039] I. Preparation of the smearing facial mask in the examples and comparative examples The formula (in mass percentage) of the smearing facial mask in examples 1-3 and comparative examples 1-9 is shown in Table 1 and Table 2. The total mass of each component in the examples and comparative examples is 100%.

[0040] Table 1 Formula composition of smearing facial mask in examples 1-3 and comparative examples 1-4 (%)

[0041] Table 2 Formula composition of smearing facial mask in comparative examples 5-10 (%)

[0042] The preparation method of the smearing facial mask of Examples 1-3 and Comparative Examples 1-8 is as follows: the kaolin, montmorillonite and diatomite are heated at 150℃ for 45 min, and after cooling to room temperature, they are crushed, passed through a 500 mesh sieve, mixed to obtain the adsorbent; the ingredients are weighed according to the mass percentage in Tables 1 and 2; the deionized water and humectant are stirred at 500 r / min for 30 min to obtain a mixture; the emulsifier is stirred at 70℃ and 800 r / min for 50 min, and then stirred with the mixture at 1500 r / min for 90 min, and after cooling to 40℃, the adsorbent, lysozyme composition, skin conditioner and preservative are added in turn at a stirring speed of 200 r / min for 30 min, and then cooled to room temperature, sterilized to obtain the smearing facial mask.

[0043] The smearing facial mask of Comparative Example 9 is prepared by the same method as that of Example 1, except that the adsorbent is not subjected to heating treatment.

[0044] The smearing facial mask of Comparative Example 10 is prepared by the same method as that of Example 1, except that the crushing particle size is 100 mesh.

[0045] II. Functional test of smearing facial mask 1. Temperature resistance stability Heat resistance: 10 g of the smearing facial mask of Examples 1-3 and Comparative Examples 1-10 is respectively placed in a transparent test bottle and kept in a (40±1)℃ dry box for 24 h, and then observed after recovery to room temperature.

[0046] Cold resistance: 10 g of the smearing facial mask of Examples 1-3 and Comparative Examples 1-10 is respectively placed in a transparent test bottle and kept in a (-8±2)℃ refrigerator for 24 h, and then observed after recovery to room temperature.

[0047] High-low temperature cycle: 10 g of the smearing facial mask of Examples 1-3 and Comparative Examples 1-10 is respectively placed in a transparent test bottle and kept in a high-low temperature alternating box with high temperature (40±0.5)℃ and low temperature (0±0.5)℃ for one cycle, and then observed after recovery to room temperature.

[0048] After 24 h of heat resistance, cold resistance and high-low cycle, the smearing facial masks of Examples 1-3 and Comparative Examples 1-10 are not layered and not agglomerated, and the texture has no obvious change compared with before the test, indicating that the smearing facial masks of Examples 1-3 and Comparative Examples 1-10 have good stability.

[0049] 2. Bacteriostasis and acne removal Propionibacterium acnes is a key factor in the pathogenesis of acne, and exists in large quantities in the acne lesion site, which can cause inflammatory reactions and lead to the occurrence of acne. Inhibiting the growth and reproduction of Propionibacterium acnes can effectively reduce inflammation and reduce the occurrence of acne. By detecting the influence of the test product on Propionibacterium acnes, the effect in the acne removal process can be directly reflected.

[0050] Test bacteria is Propionibacterium acnes Cutibacterium acnes CICC ® 10312, purchased from China Industrial Microbial Culture Collection Center.

[0051] 0.1 ml of test bacteria suspension with a concentration of 3.46 x 10 6 CFU / mL was dropped onto the surface of the nutrient agar culture medium plate and evenly coated on the entire plate surface.

[0052] 10 μL of the coating mask of Examples 1-3 and Comparative Examples 1-8 was respectively smeared on a 3 mm radius sterile filter paper disc, 10 μL of sterile saline was smeared as a negative control, the mask was pasted on the bacteria-containing plate, and the plate was cultured at 37°C for 12 h, the diameter of the bacteriostatic ring of the filter paper disc was measured, and each group was repeated for 3 times to take the average value. The specific results are shown in Table 3.

[0053] Table 3 Diameter of bacteriostatic ring (mm) of the coating mask of Examples 1-3 and Comparative Examples 1-9

[0054] Note: The diameter of the bacteriostatic ring is less than 8 mm, which is insensitive, 8-13 mm is low sensitive, 13-19 is moderate sensitive, and more than 19 mm is highly sensitive.

[0055] The negative control has no bacteriostatic ring.

[0056] As can be seen from the data in Table 3, the coating masks of Examples 1-3 and Comparative Examples 1-8 are all moderately sensitive to Propionibacterium acnes. By comparing the data of Examples 1 and Comparative Examples 1-8, it can be seen that the lysozyme, superlanzyme, superoxide dismutase, hibiscus mutabilis extract, lotus extract, peach blossom extract, magnolia kobus extract and glycyrrhiza glabra extract in the lysozyme composition have different degrees of inhibition on Propionibacterium acnes. The lysozyme composition of the present application can improve the bacteriostatic and acne-removing effects of the cleaning mask.

[0057] 3, brightening Tyrosinase is a key rate-limiting enzyme in the process of melanin synthesis. Inhibiting the activity of tyrosinase can reduce the synthesis of melanin, thereby achieving the purpose of whitening and brightening. By detecting the influence of the test product on the activity of tyrosinase, the brightening effect of the test product can be directly reflected.

[0058] Because the mud mask contains water-insoluble ingredients, which affect the absorbance determination, the inhibition rate of the lysozyme composition on tyrosinase is tested in this test.

[0059] The detection method of tyrosinase inhibition rate is as follows: The concentration of PBS buffer is 0.02 mol / L, and the pH value is 6.8.

[0060] The lysozyme compositions in Examples 1-3 and Comparative Examples 1-8 were respectively prepared into 50mg / mL solutions with PBS buffer.

[0061] Preparation of tyrosinase solution: 9.9mg of tyrosinase was accurately weighed in a 25mL volumetric flask, dissolved with 15mL of prepared PBS, and then diluted to the mark with PBS, mixed well, and stored in the dark. The dopa substrate was prepared by accurately weighing 4.93mg of tyrosine in a 25mL volumetric flask, dissolving with 15mL of prepared PBS, and then diluting to the mark with PBS, mixing well, and storing in the dark.

[0062] The optimal reaction conditions were 30℃ and the optimal pH value was 6.8. The reaction system was 3mL, and PBS and L-dopa were added according to Table 4 and incubated in a 30℃ water bath for 10min. After mixing the sample solution and the tyrosinase solution, the absorbance was measured at 475nm, and the tyrosinase inhibition rate was calculated according to the following formula: I%=[(A-D)-(B-C)] / (A-D)×100%.

[0063] The tyrosinase inhibition rates of the lysozyme compositions in Examples 1-3 and Comparative Examples 1-8 are shown in Table 5.

[0064] Table 4: Sample addition table for tyrosinase test

[0065] Table 5: Tyrosinase inhibition rates (%) of the lysozyme compositions in Examples 1-3 and Comparative Examples 1-8

[0066] As can be seen from the data in Table 5, the lysozyme compositions in Examples 1-3 and Comparative Examples 1-8 have different inhibitory effects on tyrosinase. As can be seen from the comparison of the data in Example 1 and Comparative Examples 1-3, the inhibitory effects of lysozyme, superoxide dismutase, and superoxide dismutase on tyrosinase are not obvious. As can be seen from the comparison of the data in Example 1 and Comparative Examples 4-8, the inhibitory effect of glycyrrhiza glabra extract on tyrosinase is the most obvious. Overall, the lysozyme, superoxide dismutase, superoxide dismutase, malvaviscus drummondii extract, lotus extract, peach blossom extract, magnolia kobus extract, and glycyrrhiza glabra extract in the lysozyme composition of the present application have a synergistic effect, which can significantly inhibit the activity of tyrosinase and achieve the effect of brightening the skin.

[0067] 4. Animal experiment of skin irritation Forty-five healthy adult guinea pigs weighing 250-350g were selected and divided into 9 groups, with no significant difference between the groups.

[0068] 24 hours before the experiment, the guinea pigs' back spine was shaved on both sides, about 3-5 cm on each side 2 , exposing the skin, taking care not to damage the skin.

[0069] The guinea pigs were fixed, and the skin on one side of the depilated area was evenly coated with the face mask of Example 1, Comparative Examples 1-8, and then covered and fixed with gauze and adhesive tape. The other side of the depilated area was left untreated as a blank control. The skin reactions were observed and recorded at 24 h, 48 h and 72 h after application. After each observation, the remaining test substance was gently removed with warm water, and the observation was repeated.

[0070] None of the guinea pigs in the Example 1, Comparative Examples 1-8 face mask groups and the blank control group showed erythema and edema, and there was no significant difference from the other side of the skin, i.e., no toxicity. This indicates that the face masks of Example 1, Comparative Examples 1-8 have no irritation and acute toxicity, and are safe.

[0071] 5. Clean skin feel, acne removal, and skin lightening Subjects with mild (grade I) (only comedones) and moderate (grade II) (inflammatory papules) acne were selected for the test according to the "Chinese Guide to Acne Treatment".

[0072] Exclusion criteria: pregnant or planning to become pregnant, using immunosuppressants or antihistamines, using external products or drug cosmetics to treat facial acne within 14 days, taking antibiotics to treat acne within 30 days, or having severe systemic diseases and currently taking systemic medication, and being allergic or sensitive to cosmetics / skin drugs.

[0073] Screening of subjects: 220 subjects, aged 20-45 years, were selected and divided into 11 groups, with 20 subjects in each group, and no significant difference between the subjects in each group.

[0074] Two days before the test, the subjects stopped using similar products, and after cleansing, an appropriate amount of the face mask was applied to the face (avoiding the eyes and the area around the mouth), evenly spread to a thickness of 1-2 mm, left for 10-15 min, and then rinsed with water. The application was performed every night before sleep.

[0075] (1) Clean skin feel After rinsing with water, the skin feel was evaluated in terms of skin smoothness, skin comfort and skin tightness, using a 5-point scale, with 1 point being very dissatisfied, 2 points being dissatisfied, 3 points being average, 4 points being satisfied, and 5 points being very satisfied. The scores for skin smoothness, skin comfort and skin tightness were recorded every day, and the average values were taken, as shown in Table 6.

[0076] Table 6 Evaluation of the clean skin feel of the face masks in each group

[0077] From the data of Table 6, it can be seen that the change of the components in the smearing facial mask and the preparation method have certain influence on the skin cleaning feeling. The comparison of the data of Example 1 and Comparative Examples 9-10 shows that the present application significantly improves the skin cleaning feeling of the smearing facial mask by heating and crushing the adsorbent.

[0078] (2) Oil content After 28 days, after washing the cleansing facial mask for 30 min, the forehead skin oil was measured by the multifunctional skin tester under the condition that the skin was dry and sweatless, the environmental temperature was 24℃, and the environmental relative humidity was 50%. The higher the value, the higher the oil content on the skin surface. The average skin surface oil content of each group is shown in Table 7.

[0079] Table 7 Oil content after using the smearing facial mask of each group

[0080] From Table 7, it can be seen that the change of the components in the smearing facial mask and the preparation method have different influences on the oil content in the skin. Among them, the preparation method of lysozyme and adsorbent has a greater influence on the oil content in the skin. The present application significantly reduces the oil content in the skin by adding lysozyme and heating and crushing the adsorbent, thereby achieving the cleaning effect.

[0081] (3) Acne removal After 4 weeks of use, the acne removal effect was counted according to the acne removal evaluation standard, and the specific results are shown in Table 8.

[0082] Acne removal evaluation standard: significant improvement: symptoms disappear, acne subsides; relief: symptoms are reduced, acne partially subsides or disappears; ineffective: no improvement in symptoms and acne.

[0083] Table 8 Acne removal effect of the smearing facial mask of each group

[0084] From the data of Table 8, it can be seen that the change of the components in the smearing facial mask and the preparation method have different influences on acne removal. Among them, the preparation method of lysozyme and adsorbent has a greater influence on acne removal. The present application improves the acne removal effect by adding lysozyme and heating and crushing the adsorbent.

[0085] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A lysozyme composition, characterized in that, The raw materials include the following weight parts: lysozyme 0.05-0.5 parts, suiflase 0.05-0.3 parts, superoxide dismutase 0.05-0.2 parts, malve extract 0.1-1 parts, lotus extract 0.5-1.5 parts, peach flower extract 0.5-1 parts, magnolia kobus extract 0.5-1.2 parts and glycyrrhiza glabra extract 0.5-2 parts.

2. A process for the preparation of the lysozyme composition of claim 1, characterized in that, The raw materials include the following weight parts: lysozyme 0.05-0.5 parts, suiflase 0.05-0.3 parts, superoxide dismutase 0.05-0.2 parts, malve extract 0.1-1 parts, lotus extract 0.5-1.5 parts, peach flower extract 0.5-1 parts, magnolia kobus extract 0.5-1.2 parts and glycyrrhiza glabra extract 0.5-2 parts. The lysozyme composition of claim 1 is used for preparing cosmetics. The raw materials include the following weight parts: lysozyme composition 4-6 parts, adsorbent 35-45 parts, skin conditioning agent 2-2.5 parts, skin feel modifier 6-7 parts, humectant 4-6 parts, emulsifier 3-5 parts, preservative 0.5-1.5 parts and deionized water 30-45 parts.

4. A smearing pack comprising the lysozyme composition according to claim 1, wherein The adsorbent is obtained by high-temperature treatment of kaolin, montmorillonite and diatomite.

5. The applicator mask of claim 4, wherein, The preparation method of the adsorbent includes: respectively heating kaolin, montmorillonite and diatomite at 100-200℃ for 30-60min, crushing, sieving through 300-500 mesh, and mixing according to the weight ratio (15-23):(8-13):(8-13) to obtain the adsorbent.

6. The applicator mask of claim 5, wherein, The skin conditioning agent is one or more of panthenol, ceramide 3 and ursolic acid; the skin feel modifier is one or more of squalane, ethylhexyl palmitate and cetyl PEG / PPG-10 / 1 dimethicone.

7. The applicator mask of claim 4, wherein, The humectant is one or more of sodium hyaluronate, 2-methyl-2,4-pentanediol and 1,3-butanediol; the preservative is one or more of phenoxyethanol, sodium benzoate and chlorphenesin.

8. The applicator mask of claim 4, wherein, The emulsifier is one or more of sorbitan stearate, cetylstearyl alcohol and PEG-20 stearate.

9. The applicator mask of claim 4, wherein, The raw materials include the following weight parts: lysozyme 0.05-0.5 parts, suiflase 0.05-0.3 parts, superoxide dismutase 0.05-0.2 parts, malve extract 0.1-1 parts, lotus extract 0.5-1.5 parts, peach flower extract 0.5-1 parts, magnolia kobus extract 0.5-1.2 parts and glycyrrhiza glabra extract 0.5-2 parts.

10. Process for the preparation of the spreading mask according to any one of claims 4 to 9, characterized in that, The deionized water is mixed with the humectant to obtain a mixture, the emulsifier is melted at 60-80℃ and then cooled to 35-45℃, and then the mixture, the adsorbent, the lysozyme composition, the skin conditioning agent and the preservative are mixed to obtain the smearing mask. ​

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