Compound massage essential oil for relieving pain in neck, shoulders, waist and legs and preparation method of compound massage essential oil

Abstract

The invention discloses compound massage essential oil for relieving neck, shoulder, waist and leg pain and a preparation method of the compound massage essential oil, and belongs to the technical field of traditional Chinese medicine. 8 to 12% of sandalwood; 15 to 20% of frankincense; 5-8% of Chinese ilex; 12-18% of rhizoma curcumae longae; 5-8% of hypericum perforatum; 3-5% of arnica montana; 1.5% to 2.5% of Fe3O4 (at) SiO2 nano particles; 2.5%-3.5% of a photosensitive microcapsule; and 4-6% of a ceramide composite film. The Fe3O4-coated SiO2 nanoparticles are prepared by a nitrogen protection coprecipitation method, have a magnetic targeting function, and can be enriched at a pain part under the guidance of an external magnetic field; the photosensitive microcapsule realizes accurate release of active components triggered by ultraviolet light based on the photoresponse characteristic of azobenzene wrapped by gelatin-Arabic gum; the ceramide composite membrane reconstructs a stratum corneum lipid structure through cholesterol and free fatty acid synergistically, so that the macromolecular transdermal efficiency is improved, and the skin barrier is repaired; the neck, shoulder, waist and leg pain is remarkably relieved, and the device is suitable for sensitive skin and physical therapy combined scenes.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, specifically to a compound massage oil for relieving neck, shoulder, waist and leg pain and its preparation method. Background Technology

[0002] Traditional compound essential oils often focus on simple blends of plant essential oils, such as using meadowfoam seed oil and sandalwood oil to achieve antioxidant and skin-repairing functions. However, this approach suffers from problems such as low transdermal efficiency and poor targeting of active ingredients. Current technologies often use pure herbal extracts (such as calamus and notoginseng) to relieve pain in muscle-relaxing essential oils, but these still rely on passive absorption mechanisms, making precise treatment difficult. Furthermore, conventional essential oil preparation processes (such as cold-press extraction and ethanol reflux) do not retain heat-sensitive components (such as hypericin and curcumin) sufficiently and lack sustained-release technology, resulting in short-lasting effects.

[0003] In recent years, the introduction of nanomedicine delivery systems and photoresponsive microcapsule technology has provided new ideas for the functionalization of essential oils. For example, multi-layer core-shell structures can be embedded using high-pressure microfluidics, but its application in the field of essential oils is still limited by complex processes and insufficient stability. Although existing ceramide composite membrane technology can improve the skin barrier repair effect, it has not been combined with dynamic regulation technologies such as magnetic guidance and photocontrolled release, making it difficult to meet the synergistic needs of pain relief and tissue repair.

[0004] Therefore, there is an urgent need to develop a compound essential oil system that integrates magnetic targeting, photocontrolled active release, and efficient transdermal absorption in order to break through the technical bottlenecks of traditional formulas. Summary of the Invention

[0005] The purpose of this invention is to provide a compound massage essential oil for relieving neck, shoulder, waist and leg pain and its preparation method, which solves the problems of low transdermal efficiency, poor targeting and short duration of efficacy of existing essential oil active ingredients.

[0006] The present invention achieves the above objectives through the following technical solutions:

[0007] A compound massage essential oil for relieving neck, shoulder, waist and leg pain and its preparation method, the raw materials of which include: cypress: 10-15%; sandalwood: 8-12%; frankincense: 15-20%; wintergreen: 5-8%; turmeric: 12-18%; St. John's wort: 5-8%; arnica: 3-5%; Fe3O4@SiO2 nanoparticles: 1.5-2.5%; photosensitizing microcapsules: 2.5-3.5%; ceramide composite membrane: 4-6%; wherein, the photosensitizing microcapsules are prepared by the following steps: dissolving gelatin and gum arabic in water; adding azobenzene powder and stirring until uniformly suspended; slowly dripping the aqueous phase into olive oil; emulsifying with magnetic stirring; adjusting the pH to 4.5 with glacial acetic acid; adding formaldehyde for curing; and exposing the emulsion to ultraviolet light.

[0008] According to a preferred embodiment of the present invention, the cypress was purchased from Guangzhou Keran Trading Co., Ltd.

[0009] According to a preferred embodiment of the present invention, the sandalwood was purchased from Hebei Gucheng Incense Industry Group Co., Ltd.

[0010] According to a preferred embodiment of the present invention, the frankincense was purchased from Bozhou Fushuotang Biotechnology Co., Ltd.

[0011] In this invention, cypress (containing α-pinene) and sandalwood (containing α-santalol) synergistically enhance transdermal absorption through lipid solubility. α-Boswellic acid in frankincense reduces the release of inflammatory mediators (such as PGE2) by inhibiting the COX-2 pathway, and forms an antioxidant-anti-inflammatory dual pathway with curcumin (in turmeric).

[0012] According to a preferred embodiment of the present invention, the holly was purchased from Taian Huarong Agricultural Technology Co., Ltd.

[0013] According to a preferred embodiment of the present invention, the turmeric was purchased from Ningxia Xiangcao Biotechnology Co., Ltd.

[0014] According to a preferred embodiment of the present invention, the St. John's wort was purchased from Lanzhou Waterles Biotechnology Co., Ltd.

[0015] In this invention, methyl salicylate from holly promotes blood circulation through local stimulation, while thymol from arnica and hypericin from St. John's wort synergistically inhibit nerve pain signal transmission (TRPV1 receptor regulation), thus relieving deep muscle pain.

[0016] According to a preferred embodiment of the present invention, the arnica was purchased from Chengdu Ruifensi Biotechnology Co., Ltd.

[0017] According to a preferred embodiment of the present invention, the preparation steps of the Fe3O4@SiO2 nanoparticles include dissolving FeCl2·4H2O and FeCl3·6H2O in deoxygenated water at a molar ratio of 1:2, stirring under nitrogen protection at 80°C, adding ammonia dropwise to adjust the pH to 10-11 to obtain Fe3O4, dispersing Fe3O4 in a mixture of ethanol and water at a volume ratio of 4:1, adding TEOS and ammonia for catalytic hydrolysis, centrifuging, washing, and drying to obtain Fe3O4@SiO2 nanoparticles.

[0018] In this invention, the Fe3O4 core migrates directionally to the painful site under an external magnetic field (0.5T), while the SiO2 shell adsorbs the active ingredients of plant essential oils through silanol groups, prolonging the local drug retention time. The mesoporous structure of the nanoparticles enables the gradient release of fat-soluble components such as curcumin and α-boswellic acid, reducing fluctuations in blood drug concentration. The silanol groups (-Si-OH) on the surface of the Fe3O4@SiO2 nanoparticles form hydrogen bonds with boswellic acid, enhancing the stability of drug loading.

[0019] According to a preferred embodiment of the present invention, the FeCl2·4H2O was purchased from Guangdong Wengjiang Chemical Reagent Co., Ltd.

[0020] According to a preferred embodiment of the present invention, the FeCl3·6H2O was purchased from Wuhan Jixin Yibang Biotechnology Co., Ltd.

[0021] According to a preferred embodiment of the present invention, the TEOS was purchased from Jinhong Gas Co., Ltd.

[0022] According to a preferred embodiment of the present invention, the ethanol was purchased from Nantong Runfeng Petrochemical Co., Ltd.

[0023] According to a preferred embodiment of the present invention, the ammonia water was purchased from Tengzhou Xiangrun Chemical Co., Ltd.

[0024] According to a preferred embodiment of the present invention, the ceramide composite membrane is prepared by the following steps: ceramide is mixed with cholesterol and free fatty acids in a ratio of 1:1:1, and a solvent and a penetration enhancer are added; the oil phase and the water phase are heated to 65-70°C respectively, stirred and mixed evenly, and then homogenized and emulsified at high speed; the emulsion is coated onto the substrate and dried at 45°C for 30 min.

[0025] In this invention, ceramide NP and cholesterol are compounded in a 1:1 ratio to form a layered liquid crystal structure through hydrogen bonds, which simulates the lipid arrangement of the stratum corneum of the skin and reduces the transdermal loss of active ingredients; hydrogenated lecithin (penetration enhancer) is inserted into the lipid bilayer, temporarily expanding the intercellular spaces of keratinocytes and improving the transdermal efficiency of macromolecular components such as wintergreen and turmeric (penetration rate increased by ≥30%).

[0026] According to a preferred embodiment of the present invention, the photosensitive microcapsules are prepared by the following steps: 2g of gelatin and 1g of gum arabic are dissolved in 50ml of water and stirred at 60°C; 0.3g of azobenzene powder is added and stirred until it is uniformly suspended; the aqueous phase is slowly added dropwise to 100ml of olive oil at an oil-to-water volume ratio of 2:1; the emulsification is carried out by magnetic stirring for 30min; the pH is adjusted to 4.5 with glacial acetic acid; 5ml of formaldehyde is added and the mixture is cured for 30min; the emulsion is exposed to 365nm ultraviolet light for 10min.

[0027] In this invention, gelatin-gum arabic composite aggregates to form a pH-sensitive wall material (pH 4.5 triggers swelling). Azobenzene molecules undergo cis-trans isomerization under 365nm ultraviolet light, instantly destroying the microcapsule structure and releasing the contents. The photo-triggered release of thymol and curcumin forms a high-concentration drug pool at the painful site, synergistically enhancing the local therapeutic effect with magnetic targeting. The azobenzene isomerization rate of the photosensitive microcapsules reaches over 95% after ultraviolet irradiation, ensuring rapid response.

[0028] According to a preferred embodiment of the present invention, the gelatin was purchased from Luohe Wulong Gelatin Co., Ltd.

[0029] According to a preferred embodiment of the present invention, the gum arabic was purchased from Shanxi Jinyang Pharmaceutical Excipients Co., Ltd.

[0030] According to a preferred embodiment of the present invention, the azobenzene powder was purchased from Hubei Nuona Technology Co., Ltd.

[0031] According to a preferred embodiment of the present invention, the glacial acetic acid was purchased from Anhui Chizhou Xinyu Chemical Technology Co., Ltd.

[0032] According to a preferred embodiment of the present invention, the formaldehyde was purchased from Jinan Yifengyuan Biotechnology Co., Ltd.

[0033] According to a preferred embodiment of the present invention, the ceramide is selected from at least one of ceramide NP, ceramide AP and ceramide EOP.

[0034] According to a preferred embodiment of the present invention, the ceramide NP was purchased from Hubei Baidu Chemical Co., Ltd.

[0035] According to a preferred embodiment of the present invention, the ceramide AP was purchased from Huzhou Purui Biomedical Technology Co., Ltd.

[0036] According to a preferred embodiment of the present invention, the ceramide EOP was purchased from Well Health Technology (Wuhan) Co., Ltd.

[0037] According to a preferred embodiment of the present invention, the solvent is glycerol or squalane; the penetration enhancer is hydrogenated lecithin.

[0038] According to a preferred embodiment of the present invention, the glycerol was purchased from Shandong Pingju Biotechnology Co., Ltd.

[0039] According to a preferred embodiment of the present invention, the squalane was purchased from Shanghai Yubo Biotechnology Co., Ltd.

[0040] According to a preferred embodiment of the present invention, the hydrogenated lecithin was purchased from Shanghai Aiyan Biotechnology Co., Ltd.

[0041] According to a preferred embodiment of the present invention, the oil phase is ceramide and lipids; the aqueous phase is glycerol and water.

[0042] The present invention also provides a method for preparing the compound massage essential oil, comprising the following steps:

[0043] S1, cypress, sandalwood, frankincense, wintergreen, turmeric, St. John's wort extract, arnica extract and Fe3O4@SiO2 nanoparticle dispersion were mixed and stirred at 60℃ to form an oil phase;

[0044] S2, photosensitive microcapsules, ceramide composite membrane and grape seed oil or jojoba oil are mixed in a ratio of 1:2:7, heated to 65°C and then added to the oil phase at a uniform rate;

[0045] S3, the two-phase mixture is passed through a high-pressure homogenizer to form a nanoemulsion; the emulsion is placed in a device with a magnetic field strength of 0.5T to obtain the finished product; the finished product is packaged into brown glass bottles and sealed with nitrogen.

[0046] According to a preferred embodiment of the present invention, the pressure of the high-pressure homogenizer is 80 MPa, and the two-phase mixture is circulated three times to form a nanoemulsion with a particle size ≤200 nm.

[0047] According to a preferred embodiment of the present invention, the grape seed oil was purchased from Guizhou Hongxing Development Duyun Lvyou Co., Ltd.

[0048] According to a preferred embodiment of the present invention, the jojoba oil was purchased from Jiangxi Baolin Natural Fragrance Co., Ltd.

[0049] According to a preferred embodiment of the present invention, the high-pressure homogenizer was purchased from Changzhou Dedu Precision Instruments Co., Ltd.

[0050] The present invention also provides an application of the compound massage oil in relieving neck, shoulder, waist and leg pain.

[0051] The beneficial effects of this invention are as follows:

[0052] This invention significantly improves the targeting, transdermal efficiency, and sustained effects of massage oils through multi-component synergy and formulation technology innovation. The fat-soluble components of cypress and sandalwood synergistically enhance transdermal absorption, while the active molecules of frankincense and turmeric exert a dual anti-inflammatory and analgesic effect by inhibiting inflammatory pathways and scavenging free radicals. The local stimulation and neuromodulation functions of holly and St. John's wort further alleviate deep muscle pain.

[0053] Fe3O4@SiO2 nanoparticles, guided by an external magnetic field, precisely locate pain areas, and their surface-modified mesoporous structure continuously releases active ingredients, prolonging local drug retention time. Photosensitive microcapsules utilize the rapid isomerization characteristics of photoresponsive materials in response to ultraviolet light to achieve spatiotemporally controllable release of active ingredients, overcoming the environmental dependence limitations of traditional sustained-release technologies. Ceramide composite membranes reconstruct the stratum corneum barrier by mimicking the skin's lipid structure, combined with the channel-opening effect of penetration enhancers, improving the transdermal efficiency of macromolecular active ingredients and reducing irritation.

[0054] In terms of preparation process, high-pressure homogenization combined with magnetic field solidification forms a highly stable nanoemulsion, avoiding the damage to heat-sensitive components caused by traditional processes. At the same time, the application of photosensitive microcapsules and ceramide system greatly improves product compatibility, combining barrier repair and precise drug delivery functions, and expanding the application potential of essential oils in the combined application scenarios of sensitive skin populations and physical therapy devices. Detailed Implementation

[0055] The present application will now be described in further detail. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0056] I. Implementation Examples

[0057] Example 1

[0058] 1. Preparation of Fe3O4@SiO2 nanoparticles

[0059] Step details:

[0060] (1) Weigh 9.92g FeCl2·4H2O and 26.96g FeCl3·6H2O, dissolve them in 200ml deoxygenated water (pre-boiled and deoxygenated with nitrogen), place them in an 80℃ constant temperature water bath, continuously purge with nitrogen for protection, and stir at 500rpm for 30 minutes.

[0061] (2) Add 15 ml of ammonia dropwise until the solution pH = 10.5, and continue the reaction for 1 hour to generate black Fe3O4 precipitate.

[0062] (3) Centrifuge (8000 rpm, 10 minutes) to collect the precipitate and wash with deionized water until neutral.

[0063] (4) Disperse the Fe3O4 precipitate in a mixture of 160ml ethanol and 40ml water, add 5ml TEOS (tetraethyl orthosilicate) and 2ml ammonia, and hydrolyze at 50℃ for 4 hours.

[0064] (5) Centrifuge (10000 rpm, 15 minutes), wash with ethanol 3 times, and vacuum dry at 60℃ for 12 hours to obtain 20g of Fe3O4@SiO2 nanoparticles (particle size 50±5nm).

[0065] 2. Preparation of photosensitive microcapsules

[0066] Step details:

[0067] (1) Dissolve 6.0g of gelatin and 3.0g of gum arabic in 150ml of deionized water and stir in a 60℃ water bath until completely dissolved.

[0068] (2) Add 0.9g of azobenzene powder and ultrasonically disperse for 10 minutes (200W power) to form a uniform suspension.

[0069] (3) Slowly drip the suspension into 300ml of olive oil at a rate of 5ml / min, while simultaneously emulsifying with magnetic stirring at 1000rpm for 30 minutes.

[0070] (4) Add 2ml of glacial acetic acid to adjust the pH of the emulsion to 4.5, then add 15ml of formaldehyde solution and stir to solidify for 30 minutes.

[0071] (5) The emulsion was subjected to 365nm ultraviolet light (10mW / cm²). 2 Irradiate for 10 minutes, centrifuge (4000 rpm, 5 minutes) to collect microcapsules, wash with ethanol and dry to obtain 30g of photosensitizing microcapsules.

[0072] 3. Preparation of ceramide composite membrane

[0073] Step details:

[0074] (1) Weigh 16.7g of ceramide NP, 16.7g of cholesterol and 16.7g of linoleic acid, add them to 30g of squalane, and stir in a 70°C water bath until completely dissolved.

[0075] (2) Mix 10g of glycerol with 50ml of deionized water and keep it at 70℃ to form an aqueous phase.

[0076] (3) Add the aqueous phase to the oil phase at a rate of 10 ml / min and homogenize and emulsify at 15000 rpm for 10 minutes.

[0077] (4) The emulsion was coated onto a polypropylene film, dried at 45°C for 30 minutes, and then pulverized to obtain 50g of ceramide composite film.

[0078] 4. Preparation of compound essential oils

[0079] Step details:

[0080] (1) Oil phase mixing:

[0081] Weigh out 120g of cypress extract, 100g of sandalwood extract, 180g of frankincense extract, 60g of holly extract, 150g of turmeric extract, 60g of St. John's wort extract, and 40g of arnica extract in sequence, add them to a reaction vessel, and stir at a constant temperature of 60℃ for 20 minutes.

[0082] Add 20g of Fe3O4@SiO2 nanoparticles and continue stirring for 1 hour until evenly dispersed.

[0083] (2) Functional component premixing:

[0084] Mix 30g of photosensitizing microcapsules, 50g of ceramide composite membrane and 190g of jojoba oil, preheat at 65℃ and stir until uniform.

[0085] (3) Two-phase fusion:

[0086] The premixed functional components were added to the oil phase at a rate of 20 ml / min and stirred continuously at 65°C for 40 minutes.

[0087] (4) High-pressure homogenization:

[0088] The mixture was circulated three times by an 80MPa high-pressure homogenizer, with an outlet temperature ≤35℃, to obtain a nanoemulsion (particle size 180±20nm).

[0089] (5) Magnetic field solidification:

[0090] The emulsion was injected into a 0.5T magnetic field device and left to stand for 2 hours to allow the Fe3O4@SiO2 nanoparticles to align in a specific direction.

[0091] (6) Store in separate containers:

[0092] The finished product is dispensed into brown glass bottles (50ml each), sealed with nitrogen, and stored away from light.

[0093] Example 2

[0094] The specific implementation method is the same as in Example 1, except that the content of ceramide composite membrane is increased to 60g, the solvent is replaced with glycerol, the high-pressure homogenization pressure is adjusted to 70MPa, and the number of cycles is increased to 4.

[0095] Example 3

[0096] The specific implementation method is the same as in Example 1, except that the content of azobenzene in the photosensitive microcapsule is increased to 0.5g, the ultraviolet light irradiation time is extended to 15min, and the content of Fe3O4@SiO2 nanoparticles is reduced to 15g.

[0097] Comparative Example 1

[0098] The specific implementation method is the same as in Example 1, except that Fe3O4@SiO2 nanoparticles are removed, while the remaining components are the same as in Example 1.

[0099] Comparative Example 2

[0100] The specific implementation method is the same as in Example 1, except that the photosensitive microcapsules are replaced with conventional gelatin microcapsules (without azobenzene); and there is no ultraviolet light triggering step.

[0101] Comparative Example 3

[0102] The specific implementation method is the same as in Example 1, except that the ceramide composite membrane is removed and the penetration enhancer is directly added to the oil phase.

[0103] III. Performance Testing

[0104] The compound massage oils prepared in Examples 1-3 and Comparative Examples 1-3 were subjected to performance testing according to the following methods:

[0105] 1. Transdermal efficiency test

[0106] Sample preparation: Isolated pig skin (0.8±0.1 mm thick) was collected, subcutaneous fat was removed, and the sample was equilibrated with PBS buffer for 24 hours. Experimental conditions: effective diffusion cell area 1.77 cm². 2 The receiving solution was 30% ethanol-PBS (pH 7.4), the temperature was 32±0.5℃, and the magnetic stirring speed was 600 rpm. Detection method: Samples were taken at 0.5, 1, 2, 4, 8, and 12 hours, and curcumin was detected by HPLC (mobile phase methanol-0.1% phosphoric acid = 65:35, detection wavelength 425 nm). Data processing: The cumulative permeation per unit area was calculated (Qn = Cn × V + ΣCi × Vi, where Cn is the concentration of the nth sample and V is the volume of the receiving cell).

[0107] 2. Targeted testing (MRI dynamic tracking)

[0108] Animal model: An inflammation model was established in SD rats after hair removal on their backs (carrageenan-induced); Magnetic field guidance: A 0.5T neodymium magnet was fixed 1 cm above the application site, and small animals were scanned with MRI (T2-weighted sequence, TR=3000ms, TE=80ms); Data analysis: The signal attenuation rate of the ROI region was calculated using ImageJ (ΔSI%=(SIpre-SIpost) / SIpre×100%), and the difference between the magnetic field group and the non-magnetic field group was compared.

[0109] 3. Duration of efficacy test (hot plate analgesia test)

[0110] Animal grouping: Female SD rats (180-220g) were randomly divided into 6 groups (n=8), and 0.1mL of essential oil was applied to the soles of their feet; Test procedure: The hot plate was preheated to 55±0.5℃, and the latency of licking the paw or jumping was recorded (baseline value ≤10s). The test was conducted every 30 minutes until the pain threshold returned to the baseline level; Evaluation criteria: Maintenance time = total duration of pain threshold increase >50% (compared with the blank control group).

[0111] 4. Quality Control

[0112] Stability: Accelerated testing (40℃ / 75%RH, 0 / 30 / 60 / 90 / 180 days) was used to detect layering, discoloration, and retention rate of active ingredients; Microbiology: Total bacterial count (≤1000 CFU / g) and pathogenic bacteria were tested according to the "Cosmetic Safety Technical Specifications".

[0113] (2) Test results:

[0114] Table 1: Test results of each embodiment and comparative example

[0115]

[0116]

[0117] Note: * indicates a significant difference from Example 1 (p<0.05, t-test); stability testing was conducted according to GB / T 26516-2011 Accelerated Test Method; microbial testing was conducted according to QB / T 1684 Cosmetic Inspection Rules.

[0118] As shown in Table 1, through a systematic comparison of Examples 1-3 and Comparative Examples 1-3, the present invention clearly verifies the breakthrough improvements of this technical solution in terms of transdermal efficiency, targeting, and efficacy sustainability. Regarding transdermal efficiency, Examples 1-2, by regulating the lipid structure of the stratum corneum through a ceramide composite membrane, achieved a cumulative curcumin penetration of 48.7 μg / cm³. 2 and 56.1 μg / cm 2 Compared to conventional vegetable oil-based control, Example 1 (19.5 μg / cm³) 2 This technology increases the transdermal absorption rate of essential oils by 2.5-2.9 times. By reducing the permeability resistance of active ingredients, it significantly overcomes the limitations of existing essential oil transdermal efficiency (less than 30 μg / cm³). 2The industry bottleneck was addressed. Targeted improvements relied on the synergistic effect of Fe3O4@SiO2 nanoparticles and a 0.5T magnetic field. Example 1 showed an 82.5% lesion enrichment rate in dynamic MRI tracking, a 6.5-fold improvement compared to Comparative Example 1 (12.6%) without a magnetic carrier. Notably, while Comparative Example 3 achieved a targeted enrichment rate of 83.0%, the actual delivery volume was limited due to the lack of transdermal synergistic technology, confirming the necessity of using a combined transdermal-targeted system. Regarding the duration of efficacy, Example 1 employed a photosensitive azobenzene microcapsule controlled-release system, extending the analgesic duration to 6.8 hours, a 151% improvement compared to Comparative Example 2 (2.7 hours) with traditional microcapsules. This UV-triggered sustained-release mechanism, through precise control of the active ingredient release rate, overcomes the technical limitation of traditional essential oils having an action time of less than 4 hours. Comprehensive stability tests showed that Example 1 remained stable for 180 days at 40℃ / 75%RH without stratification and met microbiological standards, while the control groups generally exhibited poor stability (Comparative Example 1 stratification ≤ 30 days) or excessive microbial levels (Comparative Example 2 colony count > 10). 3 Issues such as CFU / g were addressed. These data collectively demonstrate that this technology, through the three-dimensional synergy of ceramide transdermal enhancement, magnetic targeting navigation, and intelligent sustained release, systematically overcomes the three major technical barriers of existing essential oil active ingredient delivery: low efficiency, insufficient targeting precision, and short-lived efficacy.

[0119] The embodiments described above are merely examples of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A compound massage oil, characterized in that, By weight percentage, its raw materials include: Cypress: 10-15%; Sandalwood: 8-12%; Frankincense: 15-20%; Holly: 5-8%; Turmeric: 12-18%; St. John's wort: 5-8%; Arnica: 3-5%; Fe3O4@SiO2 nanoparticles: 1.5-2.5%; Photosensitizing microcapsules: 2.5-3.5%; Ceramide composite membrane: 4-6%; The photosensitive microcapsules are prepared by the following steps: dissolving gelatin and gum arabic in water; adding azobenzene powder and stirring until uniformly suspended; slowly dripping the aqueous phase into olive oil; emulsifying with magnetic stirring; adjusting the pH to 4.5 with glacial acetic acid; adding formaldehyde for curing; and exposing the emulsion to ultraviolet light.

2. The compound massage oil according to claim 1, characterized in that, The preparation steps of the Fe3O4@SiO2 nanoparticles include dissolving FeCl2·4H2O and FeCl3·6H2O in deoxygenated water at a molar ratio of 1:2, stirring under nitrogen protection at 80°C, adding ammonia dropwise to adjust the pH to 10-11 to obtain Fe3O4, dispersing Fe3O4 in a mixture of ethanol and water at a volume ratio of 4:1, adding TEOS and ammonia for catalytic hydrolysis, centrifuging, washing, and drying to obtain Fe3O4@SiO2 nanoparticles.

3. The compound massage oil according to claim 1, characterized in that, The ceramide composite membrane is prepared by the following steps: ceramide is mixed with cholesterol and free fatty acids in a ratio of 1:1:1, and solvent and penetration enhancer are added; the oil phase and water phase are heated to 65-70℃ respectively, stirred and mixed evenly, and then homogenized and emulsified at high speed; the emulsion is coated onto the substrate and dried at 45℃ for 30 min.

4. The compound massage oil according to claim 1, characterized in that, The photosensitive microcapsules were prepared by the following steps: 2g of gelatin and 1g of gum arabic were dissolved in 50ml of water and stirred at 60℃; 0.3g of azobenzene powder was added and stirred until it was uniformly suspended; the aqueous phase was slowly added dropwise to 100ml of olive oil at an oil-to-water volume ratio of 2:1; the emulsification was carried out by magnetic stirring for 30min; the pH was adjusted to 4.5 with glacial acetic acid; 5ml of formaldehyde was added and the mixture was cured for 30min; the emulsion was exposed to 365nm ultraviolet light for 10min.

5. The compound massage oil according to claim 3, characterized in that, The ceramide is selected from at least one of ceramide NP, ceramide AP, and ceramide EOP.

6. The compound massage oil according to claim 3, characterized in that, The solvent is glycerol or squalane; the penetration enhancer is hydrogenated lecithin.

7. The compound massage oil according to claim 3, characterized in that, The oil phase consists of ceramides and lipids; the aqueous phase consists of glycerol and water.

8. A method for preparing a compound massage essential oil according to any one of claims 1-7, characterized in that, Includes the following steps: S1, cypress, sandalwood, frankincense, wintergreen, turmeric, St. John's wort extract, arnica extract and Fe3O4@SiO2 nanoparticle dispersion were mixed and stirred at 60℃ to form an oil phase; S2, photosensitive microcapsules, ceramide composite membrane and grape seed oil or jojoba oil are mixed in a ratio of 1:2:7, heated to 65°C and then added to the oil phase at a uniform rate; S3, the two-phase mixture is passed through a high-pressure homogenizer to form a nanoemulsion; the emulsion is placed in a device with a magnetic field strength of 0.5T to obtain the finished product; the finished product is packaged into brown glass bottles and sealed with nitrogen.

9. The preparation method according to claim 8, characterized in that, The pressure of the high-pressure homogenizer is 80 MPa. The two-phase mixture is circulated three times to form a nanoemulsion with a particle size ≤200 nm.

10. The application of a compound massage oil according to any one of claims 1-7 in relieving neck, shoulder, waist and leg pain.

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