Composition for treating porcine reproductive and respiratory syndrome and feeding method thereof

Abstract

The invention discloses a composition for treating porcine reproductive and respiratory syndrome and a feeding method thereof, and belongs to the technical field of veterinary medicines. The composition is composed of veterinary acetylisovaleryltylosin tartrate, isatis root, medium chain triglyceride and glycerol monolaurate according to a specific mass ratio, the veterinary acetylisovaleryltylosin tartrate can specifically inhibit replication of porcine reproductive and respiratory syndrome viruses, the isatis root has the effects of resisting viruses and enhancing the immunity of the organism, and the medium chain triglyceride has the effect of inhibiting replication of the porcine reproductive and respiratory syndrome viruses. The mixture of medium-chain triglyceride and glycerol monolaurate not only can improve the solubility and bioavailability of effective components, but also can synergistically enhance the antiviral effect. Through scientific compounding, the composition disclosed by the invention can effectively prevent and treat the porcine reproductive and respiratory syndrome, and is high in safety and free from drug residue risk; the composition is suitable for popularization and application in large-scale pig farms and free-range households, and effectively solves the problems of single effect, low bioavailability, easy generation of drug resistance and the like of existing drugs for preventing and treating porcine reproductive and respiratory syndrome.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of veterinary medicine, in particular to a composition for treating porcine reproductive and respiratory syndrome and a feeding method thereof. BACKGROUND

[0002] Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV). The clinical manifestations mainly include maternal reproductive disorders (abortion, stillbirth, weak piglets), respiratory distress in piglets, high mortality, and respiratory symptoms in growing and finishing pigs, which has caused huge economic losses to the global pig industry. At present, the prevention and treatment of porcine reproductive and respiratory syndrome mainly includes vaccine immunization and drug treatment. However, the vaccine immunization has the problems of low strain matching degree, short immune protection period, and easy to cause immune stress. As for drug treatment, the existing technology mainly relies on single antibiotic (such as tylosin, tilmicosin, etc.) to inhibit secondary bacterial infection, which cannot directly act on the PRRSV, and long-term use may increase bacterial resistance and drug residue risk.

[0003] In addition, traditional Chinese medicine has certain potential in the prevention and treatment of porcine reproductive and respiratory syndrome. For example, radix isatidis has the effects of clearing heat and detoxifying, anti-virus, and enhancing immune function. However, the effective components of traditional Chinese medicine have poor water solubility and low bioavailability, and it is difficult to achieve ideal therapeutic effect when used alone. At the same time, the existing compositions do not consider the absorption efficiency of the effective components, which further reduces the prevention and treatment effect.

[0004] Therefore, the present application provides a composition which can synergistically inhibit PRRSV, enhance immune function, and improve drug absorption efficiency, and has high safety and no drug resistance risk, and a feeding method thereof. SUMMARY

[0005] In view of the above problems in the prior art, the present application provides a composition for preventing and treating porcine reproductive and respiratory syndrome (commonly known as "porcine reproductive and respiratory syndrome"), and a feeding application method of the composition.

[0006] A composition for treating porcine reproductive and respiratory syndrome, comprising the following ingredients by mass percentage:

[0007] 5-10% of veterinary tylosin, 15-25% of radix isatidis extract, 30-40% of medium-chain triglyceride, and 25-35% of glycerol monolaurate mixture;

[0008] The effective component content of the radix isatidis extract is ≥50%, and the glycerol monolaurate content of the glycerol monolaurate mixture is ≥80%.

[0009] Further, the preparation method of the composition comprises the following steps:

[0010] (1) the dry radix isporae coeruleae is crushed to 80-100 mesh, 70%-80% ethanol is used to reflux extract at 60-70 DEG C for 2-3h, the extract is concentrated to relative density 1.2-1.3 under reduced pressure, and radix isporae coeruleae extract is obtained;

[0011] (2) at room temperature, the medium-chain triglyceride is added into a reaction kettle, the veterinary tylosin is added under stirring, stirring is continued until complete dissolution, and mixture A is obtained;

[0012] (3) the radix isporae coeruleae extract is added into mixture A, stirring is continued for 30-40 min, the glycerol monolaurate mixture is added, and the temperature is increased to 40-50 DEG C, and stirring is continued for 1-1.5h under insulation;

[0013] (4) cooling is continued to room temperature, filtration is carried out through a 0.22 mu m microporous filter membrane, and the composition finished product is obtained.

[0014] Further, the prophylactic dosage and the therapeutic dosage are included:

[0015] (1) the prophylactic dosage: piglets are mixed with feed at a mass ratio of 1:500, 3 times a day, for 7-10d; growing and fattening pigs are mixed with feed at a mass ratio of 1:800, 2 times a day, for 10-15d; sows are mixed with feed at a mass ratio of 1:1000, for 10d in the emptying period, 7d per month in the gestation period, and 15d in the lactation period;

[0016] (2) the therapeutic dosage: piglets are mixed with feed at a mass ratio of 1:200, 3 times a day, for 10-14d, and those who cannot eat are diluted 5-10 times for gastric tube feeding, 5-10mL each time, 3 times a day; growing and fattening pigs are mixed with feed at a mass ratio of 1:400, 2 times a day, for 15-20d; sows are mixed with feed at a mass ratio of 1:500, for 20-25d, and those who have lost appetite are diluted 10-15 times for injection, 10-20mL each time, 2 times a day.

[0017] Further, the purity of the veterinary tylosin is greater than or equal to 98%.

[0018] Further, the feed is a complete compound feed for pigs.

[0019] The application has the following beneficial effects:

[0020] 1. synergistic effect of antiviral and immune, cure rate reaches 95%, significantly reduces the mortality rate, and shortens the recovery time of the pig herd;

[0021] 2. medium-chain triglyceride improves drug absorption efficiency, reduces the drug dosage, and reduces the breeding cost;

[0022] 3. the compounding scheme avoids drug resistance, the components are safe and residue-free, and meet the food safety standards;

[0023] 4. The feeding method is suitable for pigs of different ages and in different states of illness, is simple to operate, and is suitable for both large-scale and free-range scenarios;

[0024] 5. The method reduces losses such as piglet mortality and sow reproductive disorders, promotes pig weight gain, and improves economic benefits. BRIEF DESCRIPTION OF DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0026] Figure 1 The flow chart for preparing the composition for treating porcine blue ear disease according to the present application. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the following will combine the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are some of the embodiments of the present application, but not all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0028] A composition for treating porcine blue ear disease, which is composed of the following ingredients in mass percentage:

[0029] Veterinary tylosin: 5%-10%

[0030] Radix isatidis extract (effective ingredient content ≥ 50%): 15%-25%

[0031] Medium-chain triglyceride: 30%-40%

[0032] Glycerol monolaurate mixture (glycerol monolaurate content ≥ 80%): 25%-35%

[0033] Among the above-mentioned components, the veterinary tylosin is a macrolide antibiotic, which can specifically inhibit the replication process of the blue ear disease virus, and has inhibitory effect on common secondary bacterial infections (such as parahaemolyticus, streptococcus, etc.) in the pig group; the alkaloids and flavonoids in the extract of radix isatidis can directly act on the blue ear disease virus, destroy the virus structure, and can enhance the immune function of the pig body and improve the phagocytic ability of macrophages; the medium-chain triglyceride as a solvent and carrier can significantly improve the solubility of the veterinary tylosin and the extract of radix isatidis, promote the absorption of the effective components in the pig intestinal tract, and improve the bioavailability; the glycerol monolaurate mixture has broad-spectrum antiviral effect, can destroy the lipid envelope of the blue ear disease virus, prevent the virus from invading host cells, and has a synergistic effect with the veterinary tylosin and the extract of radix isatidis, further enhancing the antiviral effect.

[0034] The composition of the present application is prepared by the following steps, which is simple in operation and easy for industrialized production:

[0035] 1. Raw material pretreatment: the dried radix isatidis is crushed to 80-100 meshes, and the effective components are extracted by ethanol reflux extraction method (ethanol concentration 70%-80%, extraction temperature 60-70 DEG C, extraction time 2-3h), and the extract is concentrated to relative density 1.2-1.3 (60 DEG C) under reduced pressure to obtain the extract of radix isatidis, which is ready for use;

[0036] 2. Mixing and dissolving: the medium-chain triglyceride is added into the reaction kettle at room temperature, and after stirring uniformly, the veterinary tylosin is slowly added while stirring (stirring speed 100-150 r / min), until the veterinary tylosin is completely dissolved, to obtain a mixed solution A;

[0037] 3. Synergistic compounding: the extract of radix isatidis is added into the mixed solution A, and continues to stir for 30-40 min, then the glycerol monolaurate mixture is added, and the temperature is raised to 40-50 DEG C, and the stirring is maintained for 1-1.5h, so that the components are fully mixed;

[0038] 4. Preparation of finished product: the above-mentioned mixed solution is cooled to room temperature, filtered through a 0.22 mu m microporous filter membrane to remove impurities, to obtain a clear and transparent composition finished product, which can be directly used for subsequent feeding or prepared into a premix.

[0039] In order to ensure that the composition can exert the best prevention and treatment effect, the present application also provides a matching feeding method, which is divided into a preventive amount and a therapeutic amount according to the age and the stage of the pig group:

[0040] 1. Preventive amount (suitable for non-diseased pig group or high incidence season of blue ear disease):

[0041] Piglets (1-30 days old): the composition is uniformly mixed with the complete formula feed at a mass ratio of 1:500, and is fed 3 times a day, continuously for 7-10 days;

[0042] Growing and fattening pigs (31-180 days old): the composition is mixed with complete formula feed at a mass ratio of 1:800, and fed twice a day for 10-15 days continuously;

[0043] Sows (emptying period, gestation period, lactation period): the composition is mixed with complete formula feed at a mass ratio of 1:1000, and fed twice a day, for 10 days in the emptying period, 7 days per month in the gestation period, and 15 days continuously in the lactation period.

[0044] 2. Therapeutic dose (suitable for pigs with disease):

[0045] Piglets (1-30 days old): the composition is mixed with complete formula feed at a mass ratio of 1:200, and fed three times a day for 10-14 days continuously; if the piglets cannot feed independently, the composition can be diluted 5-10 times, and then fed through a stomach tube, 5-10 mL each time, three times a day;

[0046] Growing and fattening pigs (31-180 days old): the composition is mixed with complete formula feed at a mass ratio of 1:400, and fed twice a day for 15-20 days continuously;

[0047] Sows (disease period): the composition is mixed with complete formula feed at a mass ratio of 1:500, and fed twice a day for 20-25 days continuously; if the sow has lost appetite, the composition can be diluted 10-15 times, and then injected intravenously or intramuscularly, 10-20 mL each time, twice a day, and then changed to feed mixed with the composition when the appetite is restored.

[0048] Example 1

[0049] A composition for treating blue ear disease in pigs, the ingredients and mass percentage of which are as follows: 5% of veterinary tylosin, 25% of radix isatidis extract, 40% of medium-chain triglyceride, and 30% of glycerol monolaurate mixture.

[0050] Preparation method:

[0051] 1. The radix isatidis is ground to 80 mesh, and extracted with 70% ethanol at 60°C for 2 hours. The extract is concentrated under reduced pressure to a relative density of 1.2 (60°C) to obtain radix isatidis extract;

[0052] 2. At room temperature, the medium-chain triglyceride is added to a reaction kettle, and the veterinary tylosin is added under stirring until dissolved to obtain a mixed solution A;

[0053] 3. The radix isatidis extract is added to the mixed solution A, stirred for 30 minutes, and then the glycerol monolaurate mixture is added. The temperature is raised to 40°C, and stirred for 1 hour under heating;

[0054] 4. Cooling to room temperature, filtering through 0.22 μm microporous filter membrane to obtain the composition finished product.

[0055] Feeding method (for 30-day-old sick piglets): the composition is mixed with feed at 1:200, fed 3 times a day for 14 consecutive days; for piglets that cannot eat, dilute 5 times and gavage, 5 mL each time, 3 times a day.

[0056] Example 2

[0057] A composition for treating blue-ear disease of pigs, the ingredients and mass percentage of which are: 10% of veterinary tylosin, 15% of radix isatidis extract, 30% of medium-chain triglyceride, and 45% of glycerol monolaurate mixture.

[0058] Preparation method:

[0059] 1. The radix isatidis is crushed to 100 mesh, and 80% ethanol is used to reflux extract at 70°C for 3 hours, and the extract is concentrated under reduced pressure to a relative density of 1.3 (60°C) to obtain radix isatidis extract;

[0060] 2. At room temperature, the medium-chain triglyceride is added to the reaction kettle, and the veterinary tylosin is added under stirring, and stirring is performed until dissolution to obtain a mixed solution A;

[0061] 3. The radix isatidis extract is added to the mixed solution A, stirring for 40 minutes, and then the glycerol monolaurate mixture is added, and the temperature is increased to 50°C, and the mixture is stirred for 1.5 hours under heating;

[0062] 4. Cooling to room temperature, filtering through 0.22 μm microporous filter membrane to obtain the composition finished product.

[0063] Feeding method (for pregnant sows with disease): the composition is mixed with feed at 1:500, fed 2 times a day for 25 consecutive days; when the appetite is completely lost, intramuscular injection is performed after dilution by 10 times, 15 mL each time, 2 times a day.

[0064] In order to verify the prevention and treatment effect of the composition of the present application, the following comparative experiments are carried out:

[0065] 1. Experimental materials: 100 piglets of 28 days old with consistent clinical symptoms of blue-ear disease are selected and randomly divided into 5 groups, 20 piglets in each group, which are:

[0066] Experimental group 1: the composition of the present application (veterinary tylosin 8%, radix isatidis extract 20%, medium-chain triglyceride 35%, and glycerol monolaurate mixture 37%) is used for feed mixing according to the treatment dosage;

[0067] Control group 1: veterinary tylosin is used alone (feed mixing according to the recommended dosage);

[0068] Control group 2: using radix isatidis extract alone (mixed with feed according to recommended dosage);

[0069] Control group 3: tylosin for veterinary use + radix isatidis extract (mixed with feed according to recommended dosage);

[0070] Blank control group: without using any medicine, only feeding basic feed.

[0071] 2. Experimental index: continuously observing for 20 days, recording the cure rate, mortality rate, average recovery time and weight gain of piglets in each group.

[0072] 3. Experimental results:

[0073] Table 1

[0074] Group Cure rate (%) Mortality rate (%) Average recovery time (d) 20 d average weight gain (kg) Experimental group 1 95 5 8.2 3.8 Control group 1 60 30 12.5 2.1 Control group 2 45 40 15.3 1.8 Control group 3 75 20 10.8 2.9 Blank control group 10 85 - 0.5

[0075] The experimental results show that the cure rate of the composition of the present application is significantly higher than that of the control group and the blank control group, the mortality rate is significantly reduced, the average recovery time is obviously shortened, and the piglet weight gain effect is excellent, which fully proves the synergistic effect between the components and the significant prevention and treatment effect.

[0076] The above only describes the preferred embodiments of the present application patent and should not be used to limit the present application patent, and any modification, equivalent replacement and improvement made within the spirit and principle of the present application patent should be included in the protection scope of the present application patent.

Claims

1. A composition for treating porcine reproductive and respiratory syndrome, characterized by comprising: It is composed of the following ingredients in percentage by mass: ​ 5%-10% of veterinary tylosin, 15%-25% of radix isatidis extract, 30%-40% of medium-chain triglyceride, and 25%-35% of glycerol monolaurate mixture; The effective component content of the radix isatidis extract is ≥50%, and the glycerol monolaurate content of the glycerol monolaurate mixture is ≥80%.

2. The composition for treating porcine reproductive and respiratory syndrome according to claim 1, characterized in that: The preparation method of the composition comprises the following steps: (1) grinding dried radix isatidis to 80-100 mesh, refluxing extraction at 60-70℃ for 2-3h with 70%-80% ethanol, concentrating the extract under reduced pressure to relative density 1.2-1.3 to obtain radix isatidis extract; (2) adding medium-chain triglyceride into a reaction kettle at room temperature, stirring to add veterinary tylosin, and stirring until completely dissolved to obtain mixture A; (3) adding radix isatidis extract into mixture A, stirring for 30-40min, and then adding glycerol monolaurate mixture, heating to 40-50℃, and stirring for 1-1.5h under insulation; (4) cooling to room temperature, filtering through 0.22μm microporous filter membrane to obtain finished product of the composition.

3. A method of feeding based on the composition of claim 1, characterized in that, It includes prophylactic dosage and therapeutic dosage: (1) prophylactic dosage: for piglets, mixing the composition with feed at mass ratio 1:500, 3 times a day, for 7-10d; for growing and fattening pigs, mixing at 1:800, 2 times a day, for 10-15d; for sows, mixing at 1:1000, for 10d during emptying period, 7d per month during pregnancy period, and 15d during lactation period; (2) therapeutic dosage: for piglets, mixing the composition with feed at mass ratio 1:200, 3 times a day, for 10-14d, and for those who cannot eat, diluting 5-10 times for gastric tube gavage, 5-10mL each time, 3 times a day; for growing and fattening pigs, mixing at 1:400, 2 times a day, for 15-20d; for sows, mixing at 1:500, for 20-25d, and for those who have no appetite, diluting 10-15 times for injection, 10-20mL each time, 2 times a day.

4. The composition for treating porcine reproductive and respiratory syndrome according to claim 1, wherein, The purity of the veterinary tylosin is ≥98%.

5. The method of feeding of claim 3, wherein, The feed is full-price compound feed for pigs.

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