A plant-based composition that promotes keratin renewal and refines skin texture, and its application.

Abstract

This invention discloses a plant-based composition for promoting keratinocyte renewal and refining skin, and its application, belonging to the field of cosmetic technology. The composition comprises pomegranate fruit extract, kale leaf extract, Dendrobium officinale stem extract, and phytosterols in a specific ratio. The Dendrobium officinale stem extract is obtained using a choline chloride-oxalic acid eutectic solvent combined with microfluidic extraction. In this composition, pomegranate fruit extract promotes keratinocyte regeneration, kale leaf extract accelerates the shedding of dead skin cells, and the two synergistically regulate keratinocyte metabolism. Dendrobium officinale stem extract enhances skin's moisturizing ability, softens keratinocytes, and creates a moist renewal environment. Phytosterols repair the skin barrier and enhance the vitality of basal cells. These four components form a complete chain of action involving deep activation, metabolic regulation, and barrier repair, endogenously promoting keratinocyte renewal. It also provides exfoliation, skin refinement, long-lasting moisturizing, and barrier repair effects, and is gentle and non-irritating, making it suitable for sensitive skin.

Description

Technical Field

[0001] This invention belongs to the technical field of cosmetics, specifically relating to a plant composition that promotes keratin renewal and refines the skin, and its application. Background Technology

[0002] Abnormal thickening of the stratum corneum and accumulation of aged cells are common causes of a range of skin problems. When the stratum corneum is excessively thickened, cell metabolism slows down, causing keratin buildup around pores, leading to enlarged pores, rough and uneven skin surface, dull complexion, and dry, flaky skin. Furthermore, this abnormal buildup hinders the skin's normal absorption of skincare products, reducing their effectiveness. Additionally, the obstructed excretion of sebum can easily lead to skin infections such as acne and folliculitis.

[0003] Currently, the main methods for promoting keratinocyte renewal include chemical exfoliation and physical exfoliation. Chemical exfoliation, such as glycolic acid (alpha-hydroxy acid) and salicylic acid (beta-hydroxy acid), promotes the shedding of dead skin cells by dissolving the connections between keratinocytes; while physical exfoliation directly removes the surface keratinocytes through mechanical friction using abrasive particles, cleansing devices, etc. Although these methods can improve rough skin in the short term, they have significant limitations: overuse of irritating chemical ingredients (such as glycolic acid and trichloroacetic acid) or physical friction can easily damage the skin barrier function, leading to skin sensitivity, redness, stinging, and even post-inflammatory hyperpigmentation. Studies have shown that over-cleansing or using products containing irritating ingredients can disrupt the skin's water-oil balance, leading to compensatory thickening of the stratum corneum. Laser treatments (such as erbium YAG lasers) are highly effective, but they have drawbacks such as long downtime, high costs, and potential adverse reactions. These limitations of existing technologies have prompted researchers to seek gentler, innovative solutions that can regulate the differentiation process of keratinocytes to achieve truly healthy keratinocyte renewal and skin barrier repair. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a plant-based composition that promotes keratinocyte renewal and refines the skin. This composition utilizes pomegranate fruit extract and kale leaf extract to synergistically promote keratinocyte metabolism and renewal by promoting regeneration and accelerating shedding, respectively. Dendrobium officinale stem extract provides powerful moisturizing and softening of the stratum corneum, improving roughness and creating a favorable humid environment for keratinocyte renewal. Phytosterols enhance basal cell vitality, thicken the epidermis, and repair barrier integrity, fundamentally improving skin health and metabolic capacity, resulting in renewed, more resilient, and refined skin. These four ingredients work synergistically to endogenously promote keratinocyte renewal, achieving gentle skin rejuvenation, refinement, moisturizing, and repair effects. Furthermore, it is gentle and non-irritating, making it suitable for sensitive skin.

[0005] The specific technical solution is disclosed as follows:

[0006] In a first aspect, the present invention provides a plant composition comprising pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols in a weight ratio of (0.01-3):(0.01-5):(0.05-5):(0.001-0.5).

[0007] Preferably, the composition comprises pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols in a weight ratio of (0.1-1):(0.1-3):(0.5-3):(0.01-0.2).

[0008] Preferably, the composition comprises pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols in a weight ratio of (0.3-0.5):(0.5-1):(1-1.5):(0.05-0.1).

[0009] More preferably, the preparation method of the Dendrobium officinale stem extract includes the following steps:

[0010] The stems of Dendrobium officinale were dried, pulverized, and added to a eutectic solvent for microfluidic extraction. The product was collected, centrifuged, and the supernatant was taken. The supernatant was precipitated with alcohol, centrifuged, and the precipitate was dried to obtain the Dendrobium officinale stem extract.

[0011] The eutectic solvent is composed of choline chloride and oxalic acid.

[0012] More preferably, at least one of the following conditions is met:

[0013] (a) The molar ratio of choline chloride to oxalic acid is choline chloride: oxalic acid = 1:(2-3);

[0014] (b) The temperature of the microjets extraction is 70–90°C, or / and the homogenization pressure of the microjets extraction is 120–160 MPa;

[0015] (c) The material-to-liquid ratio of the microjet extraction is 1g:(10-30)mL.

[0016] The pomegranate fruit extract is rich in polyphenols such as ellagic acid and proteases, which accelerate epidermal renewal by stimulating the proliferation of keratinocytes and promoting the shedding of keratinocytes.

[0017] The kale leaf extract is rich in active ingredients such as sulfides and isothiocyanates, which restore epidermal homeostasis by protecting the skin and inhibiting inflammatory responses, and promote the natural shedding of dead skin cells.

[0018] The Dendrobium officinale stem extract is rich in polysaccharide components, which enhances the expression of aquaporin AQP3 to improve skin moisturizing ability and improve roughness and scaling.

[0019] Phytosterols, with their structure similar to skin cholesterol, restore the integrity of the skin barrier by regulating epidermal endogenous factors, stimulating the vitality of basal cells, and balancing the proliferation and differentiation of keratinocytes.

[0020] These four components work synergistically across multiple targets and levels to promote keratinocyte renewal and skin smoothness: pomegranate fruit extract and kale leaf extract work together to promote keratinocyte metabolism and renewal by promoting new cell growth and accelerating shedding, respectively; dendrobium officinale stem extract softens the keratinocytes and improves roughness through powerful moisturizing, creating a favorable moisturizing environment for keratinocyte renewal; and phytosterols enhance the vitality of basal cells, thicken the epidermis, and repair the barrier integrity, fundamentally improving skin health and metabolic capacity, making the renewed skin more resilient and smooth. These four components complement each other, forming a complete chain of action from deep activation and metabolic regulation to barrier repair.

[0021] This application research found that the preparation method of Dendrobium officinale stem extract, which uses optimized conditions such as low eutectic melting and microfluidic extraction, can improve extraction efficiency and also extract active components that synergistically interact with other components, thereby improving the overall effect of the plant composition.

[0022] Secondly, the present invention provides a cosmetic product comprising the plant composition described in the first aspect.

[0023] Thirdly, the present invention provides the application of the plant composition described in the first aspect in the preparation of skin care products having exfoliating, refining, moisturizing and / or repairing effects.

[0024] Fourthly, the present invention provides a skin product that promotes keratin renewal, the skin product containing the plant composition described in the first aspect;

[0025] The skin products mentioned are one or more of the following: toner, lotion, cream, mask, serum, and spray.

[0026] Fifthly, the present invention provides a lotion that promotes keratin renewal, said lotion comprising the following components in weight percentages:

[0027] Thickener 0.05-0.3%, humectant 0.5-1%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, the plant composition 1-5%, and deionized water as the balance.

[0028] Preferably, the thickener is at least one of the following: polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, sclerotium taurate gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, acrylate copolymer, and sclerotium taurate gum;

[0029] The moisturizer is at least one of the following: glycerin, 1,2-butanediol, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding stalk polysaccharide, and ceramide.

[0030] The pH adjuster is at least one of arginine, tromethamine, and disodium EDTA.

[0031] The emulsifier is at least one of the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, sodium polyacrylate, hydrogenated lecithin, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate.

[0032] The preservative is at least one of p-hydroxyacetophenone and polyol.

[0033] The beneficial effects of this invention are:

[0034] 1. The composition provided by this invention contains pomegranate fruit extract and kale leaf extract, which synergistically promote the metabolism and renewal of the stratum corneum by promoting regeneration and accelerating shedding, respectively; Dendrobium officinale stem extract effectively moisturizes and softens the stratum corneum and improves roughness, creating a good moist environment for stratum corneum renewal; and phytosterols enhance the vitality of basal cells, thicken the epidermis, and repair the barrier integrity, fundamentally improving skin health and metabolic capacity, making the renewed skin more resilient and delicate.

[0035] 2. The four ingredients provided by this invention—pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols—work synergistically to endogenously promote the renewal of skin keratinocytes, achieving gentle skin rejuvenation, refinement, moisturization, and repair. Furthermore, they are gentle and non-irritating, making them suitable for people with sensitive skin. Attached Figure Description

[0036] Figure 1 The images show a comparison of skin texture before and after 14 days of use in the blank application example and application example 1 in test example 3. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Unless otherwise specified, specific conditions in the embodiments are performed under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the components of the formulations in the embodiments are all conventional commercially available products. The following embodiments are further illustrations of the present invention, but not limitations thereof.

[0038] Unless otherwise specified, the reagents, methods and equipment used in this invention are all conventional reagents, methods and equipment in the field; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0039] In this invention:

[0040] Pomegranate fruit extract: HK-030, Shaanxi Huike Plant Development Co., Ltd.;

[0041] Kale leaf extract: GY250419, Shaanxi Guanyiyuan Biotechnology Co., Ltd.;

[0042] Phytosterols: TZ-2023071406, Peptide Biotechnology (Xi'an) Co., Ltd.;

[0043] Guava leaf extract: HK189, Shaanxi Huike Plant Development Co., Ltd.;

[0044] Sea Breeze Vine Stem Extract: HK201, Shaanxi Huike Plant Development Co., Ltd.;

[0045] Isatis leaf extract: XTYDQY, Shaanxi Xintianyu Biotechnology Co., Ltd.;

[0046] Coconut oil alcohol: 112-53-8, Nantong Maruei International Trade Co., Ltd.;

[0047] Dendrobium officinale stem: The stem of Dendrobium officinale, a species belonging to the genus Dendrobium in the family Orchidaceae, is commercially available.

[0048] Dendrobium officinale stem extract 1

[0049] Homemade, the preparation method includes the following steps:

[0050] 1) Mix choline chloride and oxalic acid in a molar ratio of 1:2 and heat to melt to obtain a eutectic solvent;

[0051] 2) After drying and pulverizing the stems of Dendrobium officinale, add them to a eutectic solvent. The mass-volume ratio of the Dendrobium officinale stems to the eutectic solvent is 1g:10mL. Then, use a microfluidic homogenizer to cycle the mixture twice at a pressure of 120MPa, a stirring speed of 600rpm, and a temperature of 90℃. After the cycle, crush the mixture and centrifuge it at 12000g for 20min. Collect the supernatant, add ethanol (final concentration 95%, v / v), and precipitate it at 4℃ for 12h. Centrifuge the precipitate and vacuum dry it at 25℃ to obtain Dendrobium officinale stem extract.

[0052] Dendrobium officinale stem extract 2

[0053] The preparation method of this self-made product differs from that of Dendrobium officinale stem extract 1 in that, in step 1), choline chloride and oxalic acid are mixed in a molar ratio of 1:3, and in step 2), the mass-volume ratio of Dendrobium officinale stem and eutectic solvent is 1g:30mL. The microfluidic extraction temperature is 70℃ and the pressure is 160MPa.

[0054] Dendrobium officinale stem extract 3

[0055] The preparation method of this self-made product differs from that of Dendrobium officinale stem extract 1 in that, in step 1), choline chloride and oxalic acid are mixed in a molar ratio of 1:4, and in step 2), the mass-volume ratio of Dendrobium officinale stem and eutectic solvent is 1g:40mL. The microfluidic extraction temperature is 60℃ and the pressure is 80MPa.

[0056] Dendrobium officinale stem extract 4

[0057] The preparation method of this self-made product differs from that of Dendrobium officinale stem extract 1 in that, in step 1), choline chloride and oxalic acid are mixed in a 1:1 molar ratio, and in step 2), the mass-volume ratio of Dendrobium officinale stem and eutectic solvent is 1 g: 5 mL. The microfluidic extraction temperature is 100 °C and the pressure is 200 MPa.

[0058] Dendrobium officinale stem extract 5

[0059] Homemade, the preparation method includes the following steps:

[0060] The stems of Dendrobium officinale were dried, pulverized, and added to a 70% ethanol aqueous solution for ultrasonic extraction. The material-to-liquid ratio was 1g:30mL. The ultrasonic extraction temperature was 40℃, the power was 120W, and the time was 30min. After extraction, the extract was centrifuged, the supernatant was collected, concentrated, and dried to obtain Dendrobium officinale stem extract 5.

[0061] Preparation of Examples

[0062] Table 1

[0063]

[0064]

[0065] The preparation method of the plant composition provided in Example 1 is as follows: the components are mixed evenly to obtain the plant composition.

[0066] The preparation methods of the plant compositions in Examples 2-11 and Comparative Examples 1-8 are consistent with those in Example 1.

[0067] Comparative Example 9

[0068] The present invention provides a plant composition in a comparative example, the only difference between the plant composition and Example 1 being that guava leaf extract is used instead of pomegranate fruit extract.

[0069] Comparative Example 10

[0070] The present invention provides a plant composition in a comparative example, the only difference between the plant composition and Example 1 being that the extract of Isatis tinctoria leaf is used instead of the extract of kale leaf.

[0071] Comparative Example 11

[0072] The present invention provides a plant composition in a comparative example, the only difference between the plant composition and Example 1 being that the extract of *Dendrobium officinale* stem is replaced with extract of *Piper kadsura* stem.

[0073] Comparative Example 12

[0074] The present invention provides a plant composition in a comparative example, the only difference between the plant composition and Example 1 being the use of coconut oil alcohol instead of phytosterol.

[0075] Preparation of application examples

[0076] The present invention provides an emulsion in application examples and comparative application examples, the components (mass percentage) of which are shown in Table 2; wherein, the plant compositions used in application examples 1-11 are the plant compositions prepared in examples 1-11 respectively, and the plant compositions used in application examples 12-13 are the plant compositions prepared in example 1; the plant compositions used in comparative application examples 1-12 are the plant compositions prepared in comparative examples 1-12 respectively; in addition, a blank application example is provided, the only difference between the blank application example and application example 1 is that no plant composition is added;

[0077] Table 2

[0078]

[0079]

[0080] This embodiment provides a method for preparing an emulsion, the method comprising the following steps:

[0081] (1) Mix the humectant, thickener and part of the pH adjuster (EDTA-disodium) with water and stir. Heat to 85°C and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared phase A.

[0082] (2) Mix the emulsifier, heat to 75°C, and homogenize at 1300 rpm for 4 min. After homogenization, keep warm for later use to obtain the pre-prepared phase B.

[0083] (3) Mix the preservatives and heat to 60°C to melt them to obtain the pre-prepared C phase;

[0084] (4) Heat the pre-prepared phase A to 80°C, add the pre-prepared phase B at 300 rpm, stir and mix, then cool down to 60°C, add the pre-prepared phase C at 300 rpm and stir and mix, then cool down to below 45°C, add the plant composition and continue stirring for 8 minutes, finally add the remaining pH adjuster (arginine) to adjust the pH to 5.5-7.0, then stop stirring, discharge the material, and obtain the emulsion.

[0085] Effect Experiment

[0086] Test Example 1: Cell experiments demonstrated that the composition promotes keratinocyte turnover.

[0087] Experimental methods:

[0088] This study investigates the effect of the compositions prepared in Examples 1-11 and Comparative Examples 1-12 on promoting keratin renewal.

[0089] The cell line used was human keratinocytes (HaCaT, Beina Biotechnology). The testing conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to groups, followed by testing. Specifically, the levels of serine protease KLK5 and transglutaminase TGM1 were detected using the following methods:

[0090] The cell suspension was seeded into 96-well cell culture plates at a density of 2000 cells / well. 100 μL of DMEM medium (2105341, Gibco) was added to each well and the cells were cultured for 24 h.

[0091] Discard the supernatant. Add 100 μL of DMEM medium to the blank control group and add 100 μL of DMEM medium containing 0.05 wt% of the corresponding plant composition (plant compositions prepared in Examples 1-11 and Comparative Examples 1-12) to the sample groups. Each group has 6 wells for parallel replication and is cultured for 24 h.

[0092] Collect cells from each group, discard the supernatant, lyse the cells with RIPA lysis buffer containing protease inhibitor (Shanghai Merck, V900854), centrifuge the lysed sample at 12000g for 5 minutes, and take the supernatant for subsequent experiments.

[0093] (4) Detection of KLK5 serine protease content: The KLK5 content of each group was detected using the KLK5 ELISA Kit (Human) (catalog number ASB-OKCD06351-96W, Xinbosheng). The specific operation was as follows: equal amounts of supernatant were added to the corresponding reaction wells of the kit. After incubation, washing, and color development, the absorbance OD value was measured at a specific wavelength using an ELISA reader. The KLK5 content of each group was calculated based on the standard curve.

[0094] (5) Detection of transglutaminase TGM1 content: The TGM1 content of each group was detected using a human transglutaminase 1 (TGM1) ELISA kit (catalog number ml 105480, ELISA Bio). The specific operation was as follows: equal amounts of supernatant were added to the corresponding reaction wells of the kit. After incubation, washing, and color development, the absorbance OD value was measured at a specific wavelength using an ELISA reader. The TGM1 content of each group was calculated according to the standard curve.

[0095] Serine protease KLK5 is a serine protease that promotes keratin exfoliation, while transglutaminase TGM1 is a key cross-linking enzyme that forms a protective keratin capsule. The two work synergistically to regulate the normal renewal of the stratum corneum and the stability of the skin barrier.

[0096] The effect of the composition on promoting keratin renewal is represented by the improvement in KLK5, and the effect on repairing the skin barrier is represented by the improvement in TGM1. The calculation method is as follows:

[0097] Improvement rate = (OD value) 样品组 -OD value 空白对照组 ) / OD value 空白对照组 ×100%;

[0098] The results are shown in Table 3.

[0099] Table 3

[0100]

[0101]

[0102] Results analysis:

[0103] The experimental results show that the improvement rates of KLK5 in Examples 1-5 reached 21.2%-24.3%, and the improvement rates of TGM1 reached 47.3%-53.8%, which are significantly higher than those of Examples 6-7 (16.4%-17.5% for KLK5 and 39.7%-42.2% for TGM1). This indicates that, within the preferred weight ratio range, the components can more efficiently and synergistically regulate keratin shedding and keratin capsule formation, achieving a balance between shedding and regeneration.

[0104] The improvement rates of KLK5 in Comparative Examples 1-2 were only 8.6%-9.4%, and the improvement rates of TGM1 were 25.8%-27.5%, which were far lower than those in the Example Group. This demonstrates that an imbalance in the component ratio leads to a significant decrease in efficacy and verifies the key role of weight ratio.

[0105] The improvement rate of KLK5 in comparative examples 3-6 was only 4.3%-7.3%, while the improvement rate of TGM1 was 14.2%-18.3%; the improvement rate of comparative examples 7-8 was also significantly lower. This indicates that pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols play an indispensable and crucial role in this formulation system.

[0106] In Examples 1 and 8, which employed a low-eutectic solvent and microfluidic extraction optimized process, the improvement rates for KLK5 reached 24.3%-24.6% and TGM1 reached 52.7%-53.8%. However, the improvement rates in Examples 9 and 10 decreased to 22.6% / 50.8% and 20.9% / 47.7%, respectively. Example 11, which used conventional ethanol ultrasonic extraction, showed an improvement rate of 19.3% / 45.1%. This indicates that the low-eutectic microfluidic extraction process provided by this invention can effectively extract active polysaccharides from Dendrobium officinale stems, enabling them to form a highly efficient synergistic effect with other components, further demonstrating the importance of process optimization for the overall efficacy of the composition.

[0107] Test Example 2: Human Skin Patch Test

[0108] Sixty volunteers were recruited, 30 men and 30 women, aged 20-50 years, and randomly divided into two groups of 15 men and 15 women each. A closed patch test was used. Equal volumes (0.020 mL–0.025 mL) of the test sample (emulsions prepared in Application Examples 1–13, Control Application Examples 1–12, and the blank application example) were placed in a specific patch applicator. The patch was applied to the volunteer's arm with hypoallergenic adhesive tape, and gently pressed to ensure even application to the skin. The patch was left on for 24 hours. The blank control group used distilled water, and the blank application examples used an emulsion without plant-based ingredients. One patch applicator was applied to each volunteer's arm, and each applicator had 10 wells, allowing for the simultaneous testing of 20 samples per volunteer (both arms). The first group used emulsions prepared in Application Examples 1–11 and the blank application example, while the second group used emulsions prepared in Control Application Examples 1–12 and the blank application example. A blank control was included in each group. After 24 hours, the patch applicator was removed, and skin reactions were observed at 0.5 hours, 24 hours, and 48 hours, with results recorded. The severity of adverse skin reactions is shown in Table 4 below.

[0109] Table 4

[0110]

[0111] After testing, the lotions provided in Application Examples 1-13, Comparative Application Examples 1-12, and Blank Application Examples of the present invention all showed negative reactions after human patch testing, indicating that they are safe and non-irritating to human skin.

[0112] Test Example 3: Human efficacy test of the composition in exfoliation, skin smoothing, moisturizing, and repair.

[0113] Experimental methods:

[0114] 1. Experimental basis:

[0115] The human efficacy evaluation test method shall be followed in accordance with the "Technical Specifications for Cosmetic Safety" (2015 edition).

[0116] 2. Subject screening:

[0117] Inclusion criteria: We are recruiting Asian adult volunteers aged 18-60 who meet the following conditions:

[0118] a) The individual perceives rough facial skin, with a skin texture feature count > 1000 and a skin texture score > 5.0;

[0119] b) A self-reported history of skin allergies (confirmed by a dermatologist);

[0120] c) No history of serious systemic diseases or skin diseases.

[0121] Exclusion criteria: pregnant / lactating women, individuals with severe allergies, and individuals who have participated in other clinical trials within the past 3 months.

[0122] Number of participants: A total of 130 qualified volunteers were included and divided into 26 groups of 5 people each using a random number table.

[0123] 3. Sample application method:

[0124] Test samples: emulsions prepared in Application Examples 1-13, Comparative Application Examples 1-12, and Blank Application Examples (double-blind method numbering).

[0125] How to use: After cleansing in the morning and evening, volunteers should take 1mL of the sample and apply it evenly to the entire face, gently massaging until fully absorbed.

[0126] 4. Testing cycle and process:

[0127] Test period: 14 days (D0 to D14).

[0128] Visit time points: Instrument testing was conducted at D0 (baseline period) and D14 (end of the test).

[0129] Preparation before testing:

[0130] a) After the participants arrived, they used a uniform, non-irritating facial cleanser to clean their faces;

[0131] b) Rest for 30 minutes in a constant temperature and humidity environment (temperature 21±1℃, humidity 50±10%);

[0132] c) Keep your eyes closed and relax during the test to avoid facial expressions and movements that may interfere with the process.

[0133] 5. Testing instruments and test indicators:

[0134] Before testing, the instruments were calibrated to standard parameters. Researchers used the CK GL200 skin gloss test probe to measure the gloss of the volunteers' cheekbones; used VISIA-CR to photograph and analyze the volunteers' skin texture; used the Corneometer skin moisture test probe to measure the skin moisture content of the volunteers' cheeks; and used the TewaMeter skin moisture loss test probe to measure the transepidermal water loss (TEWL) value of the volunteers' cheekbones. During the test, probe pressure and measurement time were kept constant, and measurements were repeated three times at the same location, with the average value taken.

[0135] The exfoliating ability of the samples is represented by the improvement in skin radiance; the skin smoothing effect is represented by the improvement in skin texture score; the moisturizing effect is represented by the improvement in skin moisture content; and the skin barrier repair ability is represented by the improvement in transepidermal water loss (TEWL) value. The formulas are as follows:

[0136] Gloss improvement rate = (gloss value) T14 - Glossiness T0 Gloss T0 ×100%;

[0137] Skin texture score improvement rate = (skin texture score) T0 -Skin texture score T14 Skin texture score T0 ×100%;

[0138] Skin moisture content improvement rate = (skin moisture content) T14 -Skin moisture content T0 ) / Skin moisture content T0 ×100%;

[0139] TEWL value improvement rate = (TEWL value) T0 -TEWL value T14 ) / TEWL value T0 ×100%;

[0140] The results are shown in Table 5.

[0141] Table 5

[0142]

[0143]

[0144] Results analysis:

[0145] Application Examples 1-5 showed improvements in gloss (17.2%-18.8%), skin texture (40.1%-43.5%), moisture content (62.5%-66.2%), and TEWL value (19.5%-21.1%), demonstrating a significant improvement in efficacy compared to the blank application examples. Figure 1 These are comparative photographs of skin texture analyzed by a VISIA-CR instrument after volunteers in Application Example 1 and the blank application example used the product for 14 days. The above results demonstrate that the composition provided by this invention can effectively achieve four effects: exfoliation, refinement, moisturizing, and repair.

[0146] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A plant composition, characterized in that, The plant composition consists of pomegranate fruit extract, kale leaf extract, dendrobium stem extract, and phytosterols in a weight ratio of (0.1-1):(0.1-3):(0.5-3):(0.01-0.2).

2. The plant composition according to claim 1, characterized in that, The plant composition consists of pomegranate fruit extract, kale leaf extract, dendrobium officinale stem extract, and phytosterols in a weight ratio of (0.3-0.5):(0.5-1):(1-1.5):(0.05-0.1).

3. The plant composition according to claim 1 or 2, characterized in that, The preparation method of the Dendrobium officinale stem extract includes the following steps: The stems of Dendrobium officinale were dried, pulverized, and added to a eutectic solvent for microfluidic extraction. The product was collected, centrifuged, and the supernatant was taken. The supernatant was precipitated with alcohol, centrifuged, and the precipitate was dried to obtain the Dendrobium officinale stem extract. The eutectic solvent is composed of choline chloride and oxalic acid.

4. The plant composition according to claim 3, characterized in that, Satisfy at least one of the following: (a) The molar ratio of choline chloride to oxalic acid is choline chloride: oxalic acid = 1: (2-3); (b) The temperature of the microjets extraction is 70–90°C, or / and the homogenization pressure of the microjets extraction is 120–160 MPa; (c) The material-to-liquid ratio of the microjet extraction is 1g:(10-30)mL.

5. The use of the plant composition according to any one of claims 1-4 in a skin care product having exfoliating, refining, moisturizing and / or repairing effects.

6. A cosmetic product, characterized in that, The cosmetic product comprises the plant composition according to any one of claims 1-4.

7. A skin product that promotes keratin renewal, characterized in that, The skin product contains the plant composition according to any one of claims 1-4; The skin products mentioned are one or more of the following: toner, lotion, cream, mask, serum, and spray.

8. A lotion that promotes keratin renewal, characterized in that, The emulsion consists of the following components in weight percentage: Thickener 0.05-0.3%, humectant 0.5-1%, pH adjuster 0.01-0.3%, emulsifier 0.5-5%, preservative 0.5-3%, plant composition according to any one of claims 1-4 1-5%, deionized water balance.

9. The emulsion according to claim 8, characterized in that, The thickener is at least one of the following: carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, sclerotium tauren gum, guar gum, ammonium acryloyl dimethyl taurate / VP copolymer, acrylate copolymer, and sclerotium tauren gum; The moisturizer is at least one of the following: glycerin, 1,2-butanediol, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, caprylyl glycol, dipropylene glycol, tremella polysaccharide, trehalose, betaine, allantoin, sodium hyaluronate, β-glucan, budding stalk polysaccharide, and ceramide. The pH adjuster is at least one of arginine, tromethamine, and disodium EDTA. The emulsifier is at least one of the following: caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, sodium polyacrylate, hydrogenated lecithin, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate. The preservative is at least one of p-hydroxyacetophenone and polyol.

Images