Preparation process of transparent bag for cell resuscitation

By optimizing the preparation process of transparent cell resuscitation bags, using high-purity FEP particles and precise hot-melt molding, cutting, and sterilization, the problems of low yield and poor stability in existing technologies have been solved, achieving the preparation of high-quality transparent cell resuscitation bags and improving the safety and success rate of cell resuscitation.

CN121105448APending Publication Date: 2025-12-12XIAN CHUYUAN SAIER BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511301880.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-12
Publication Date
2025-12-12

AI Technical Summary

Technical Problem

Existing cell resuscitation transparent bag preparation processes result in low yields, cannot maintain stability and transparency in a -196℃ liquid nitrogen cryogenic environment, and pose a risk of leaching of harmful substances, affecting cell resuscitation efficacy.

Method used

Using medical-grade FEP particles with a purity of ≥99.9% as raw materials, the process involves vacuum drying, screening, hot-melt film pressing, CNC laser cutting, heat sealing, and gamma ray sterilization to ensure the purity and absence of impurities in the raw materials. Combined with five-stage temperature gradient extrusion, three-stage cooling, and high-precision cutting, a uniform and smooth film is formed. After assembly and sterilization via Luer connectors, a high-quality transparent cell resuscitation bag is formed.

Benefits of technology

This improved the yield of transparent cell resuscitation bags, ensured they did not rupture at low temperatures, maintained good transparency, reduced the leaching of harmful substances, and enhanced the safety and success rate of cell resuscitation.

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Abstract

The invention discloses a preparation process of a transparent bag for cell resuscitation, which comprises the following steps of: 1, preparing raw materials: selecting FEP (Fluorinated Ethylene Propylene) particles meeting the biocompatibility requirement as a main raw material, and preferentially selecting a medical-grade raw material with the purity of more than or equal to 99.9 percent; after the raw materials are selected, FEP particles are put into a vacuum drying oven and dried for 4-6 hours under the conditions that the temperature is 80-100 DEG C and the vacuum degree is smaller than or equal to-0.09 MPa, residual moisture in the raw materials is removed, and the situation that bubbles are generated during extrusion to affect the quality of the thin film is avoided; and 2, hot melting and film pressing: putting the FEP particles dried in the step 1 into an extruder, and melting the FEP particles. Then, the molten FEP is extruded into a film shape through an extruding machine head, the film is subjected to thickness adjustment and surface flattening treatment through a calendering device, and an FEP film with a certain thickness and width is prepared; according to the invention, FEP particles are pretreated, and then five-stage gradient temperature degradation and three-stage cooling of the extruder are carried out, so that the prepared film is uniform and smooth, and the rejection rate is reduced.
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