Pesticide composition and application thereof

Abstract

The invention belongs to the field of pesticides, and relates to a pesticide composition and application, the pesticide composition comprises an active component A and an active component B, the active component A is any one of imidacloprid, dinotefuran and nitenpyram, the active component B is a compound of formula (I), and the mass ratio of the active component A to the active component B is 1: 64-60: 1. The pesticide composition disclosed by the invention has an excellent prevention and treatment effect on hemiptera pests, has a remarkable synergistic effect under a certain mass ratio, can delay the generation of drug resistance of the pests, and is safe to the environment.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition containing neonicotinoids. Background Technology

[0002] Neonicotinic insecticides are broad-spectrum and highly effective insecticides for controlling piercing-sucking insects, small lepidopteran and coleopteran pests. Their primary site of action is the nicotinic acetylcholine receptor. Once absorbed by organisms, neonicotinic pesticides rapidly bind to nicotinic acetylcholine receptors (nAChRs) in the nervous system, blocking receptor binding and disrupting neurobehavioral toxicity, leading to paralysis, death, or even death in the insects. However, the excessive, frequent, and inappropriate use of neonicotinic insecticides has exacerbated the evolution of insecticide resistance in pests, making control more difficult and causing more severe damage to crops.

[0003] With increasing emphasis on agricultural ecological security, reducing the use of chemical pesticides, implementing "green pest control" strategies, and building environmentally friendly agriculture have become important development directions for plant protection science. This invention demonstrates excellent control effects on various pests by mixing imidacloprid, dinotefuran, and acetamiprid with compounds of formula (I), exhibiting significant synergistic effects and reducing the development of pesticide resistance in pests. Summary of the Invention

[0004] The purpose of this invention is to provide a pesticide composition containing neonicotinoid compounds that has synergistic effects, reduces resistance, and has low usage costs.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a pesticide composition containing neonicotinoids, comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I).

[0006] A pesticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is any one of imidacloprid, dinotefuran, and acetamiprid, and active ingredient B is a compound of formula (I);

[0007] The mass ratio between active ingredient A and active ingredient B is 1:64 to 60:1, such as any ratio of 1:64, 1:58, 1:52, 1:50, 1:45, 1:35, 1:30, 1:24, 1:20, 1:16, 1:12, 1:11, 1:10, 1:6, 3:7, 1:2, 5:1, 7:1, 10:1, 14:1, 15:1, 22:1, 30:1, 35:1, 45:1, 55:1, 60:1.

[0008] The mass ratio of active ingredient A to active ingredient B is 1:58 to 35:1;

[0009] The mass ratio of the imidacloprid to the compound of formula (I) is 1:45 to 30:1;

[0010] The mass ratio of the imidacloprid to the compound of formula (I) is 1:45, 1:30, 1:24, 1:12, 1:2, 7:1, 14:1, or 30:1;

[0011] The mass ratio of the fipronil to the compound of formula (I) is 1:58 to 30:1;

[0012] The mass ratio of the fipronil to the compound of formula (I) is 1:58, 1:30, 1:20, 1:10, 3:7, 10:1, 22:1, or 30:1;

[0013] The mass ratio of the acetamiprid to the compound of formula (I) is 1:50 to 35:1;

[0014] The mass ratio of the acetamiprid to the compound of formula (I) is 1:50, 1:35, 1:16, 1:11, 1:6, 1:2, 5:1, 10:1, 15:1, or 35:1;

[0015] Furthermore, based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5% to 90%.

[0016] Furthermore, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1% to 85%;

[0017] Furthermore, in addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

[0018] Furthermore, the pesticide composition further includes an adjuvant selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, and carriers;

[0019] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0020] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0021] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0022] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0023] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0024] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0025] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0026] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0027] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0028] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0029] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0030] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0031] Furthermore, the pesticide composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from solid and / or liquid formulations;

[0032] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0033] Furthermore, the liquid formulation includes soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions;

[0034] Furthermore, the solid dosage form is selected from wettable powders and water-dispersible granules; the liquid dosage form is selected from emulsifiable concentrates, water-in-oil emulsions, microemulsions, suspensions, suspension emulsions, and dispersible oil suspensions.

[0035] The application of the pesticide composition described in this invention in the control of agricultural and forestry pests and sanitary pests;

[0036] The pesticide composition described in this invention can be used to control pests on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops.

[0037] Furthermore, the aforementioned agricultural and forestry pests and sanitary pests are pests belonging to the orders Hemiptera, Thysanoptera, and Lepidoptera.

[0038] Furthermore, the aforementioned Hemiptera pests include: green stink bugs (Acrosternum hilare), chinch bugs (Blissus leucopterus), potato blind bugs (Calocoris norvegicus), aphids, scales, whiteflies, leafhoppers, pea aphids (Acrythosiphon pisum), Adelges spp., cabbage whiteflies (Aleurodes proletella), and spiral whiteflies (Aleurodicus). The following species are listed: * *disperses*, *Aleurothrixus floccosus* (woolly whitefly), *Aluacaspis spp.*, *Aonidiella aurantii*, *Aphis spp.*, *Aphis gossypii* (cotton aphid), *Aphis pomi*, *Aulacorthum solani* (foxglove aphid), *Bemisia spp.* (whitefly), *Bemisia argentifolii*, *Bemisia tabaci* (sweet potato whitefly), *Brachycolus noxius*, *Brachycorynella asparagi* (asparagus aphid), *Brevennia rehi*, and *Brevicoryne*. Species of the genera *Ceroplastes* (brassicae), *Ceroplastes spp.*, *Ceroplastes rubens* (red wax scale), *Chionaspis* (spp.), *Chrysomphalus* (spp.), *Dysaphis plantaginea* (rosy apple aphid), and *Empoasca* (green leafhopper).Apple cotton aphid (Eriosomalanigerum), cottony cushion scale (Icerya purchasi), mango leafhopper (Idioscopus nitidulus), planthopper (Laodelphaxstriatellus), oyster scale (Lepidosaphes spp.), long-tubed aphid (Macrosiphum spp.), euphorbiae, granarium, rosae, quadrilineatus (aster leafhopper), frimbiolata, dirhodum, longicornis, myzus spp., persicae (green peach aphid) The following species are listed: *Nephotettix* spp., *Nephotettix cinctipes* (green leafhopper), *Nilaparvata lugens*, *Parlatoria pergandii*, *Peregrinus maidis* (corndelphacid), *Philaenus* spp., *Phylloxeravitifoliae* (grape phylloxera), *Physokermes piceae* (spruce bud scale), *Planococcus* spp. (mealyptus), *Pseudococcus* spp. (mealyptus), and *Pseudococcus brevipes* (pineapple mealybug). The species *Mealybug*, *Quadraspidiotus perniciosus* (San Josescale), and *Rhopalosiphum spp.*The following aphids are listed: * *Rhapalosiphum maida* (corn leaf aphid), * *Rhapalosiphum padi* (oat bird-cherry aphid), * *Saissetia spp.*, * *Saissetia oleae*, * *Schizaphis graminum* (green bug), * *Sitobion avenae* (English grain aphid), * *Sogatella furcifera*, * *Therioaphis spp.*, * *Toumeyella spp.*, * *Trialeurodes spp.*, and * *Trialeurodes vaporariorum*.

[0039] The present invention also provides a method of using the pesticide composition described above, specifically applying it in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

[0040] The pesticide composition of the present invention has the following advantages:

[0041] 1) The pesticide composition of the present invention has the characteristics of significant synergistic effect within a certain ratio range, broad insecticidal spectrum, and can control a variety of pests with significant control effect;

[0042] 2) The pesticide composition of the present invention can delay the development of resistance and extend the service life of a single pesticide agent. Detailed Implementation

[0043] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0044] The compositions of the present invention can be provided in formulation form. They can be formulated as suspensions, water-dispersible granules, emulsions, pellets, wettable powders, dispersible oil suspensions, etc., as needed. The content of the active ingredient in the compositions of the present invention depends on the application rate when used alone, as well as on the mixing ratio and the degree of synergistic effect. The optimal range of active ingredient content varies depending on the formulation type of the composition.

[0045] Formulation preparation examples

[0046] Example 1:

[0047] 25% Formula (I) Compound·Imidacloprid Suspension Concentrate (15:10)

[0048] Formula: 15% compound of formula (I), 10% imidacloprid, 3% fatty alcohol polyoxyethylene ether, 1.5% lignin sulfonate, 4% styrene phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 1.2% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0049] Preparation method: Add the active ingredient to the wetting and dispersing agent and defoamer, use zirconia beads, and wet pulverize with a sand mill to D. 90 (90% of the particles have a particle size) < 5 μm to obtain a pulverized slurry. Add other additives to the pulverized slurry and mix thoroughly. Add deionized water to make up to 100%, and shear at high speed to obtain a suspension product.

[0050] Example 2:

[0051] 20% Formula (I) Compound·Fipronil Suspension (14:6)

[0052] Formula: 14% compound of formula (I), 6% fipronil, 1.5% lignin sulfonate, 3% isotridecyl alcohol polyoxyethylene ether, 1% naphthalene sulfonate formaldehyde condensate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4.5% styrene phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 1.2% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0053] Preparation method: Same as in Example 1.

[0054] Example 3:

[0055] 26% Formula (I) Compound·Acetaminophen Suspension (14:12)

[0056] Formula: 14% compound of formula (I), 12% acetamiprid, 3.5% isotridecyl alcohol polyoxyethylene ether, 1% naphthalene sulfonate formaldehyde condensate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4.5% styrene phenol polyoxyethylene ether phosphate, 0.5% magnesium aluminum silicate, 0.25% xanthan gum, 1.2% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0057] Preparation method: Same as in Example 1.

[0058] Example 4:

[0059] 40% Formula (I) Compound·Imidacloprid Wettable Powder (25:15)

[0060] Formula: 25% compound of formula (I), 15% imidacloprid, 5% naphthalenesulfonate formaldehyde condensate, 10% tea saponin, 2% sodium dodecyl sulfate, 2% starch, 10% kaolin, and bentonite to make up the balance;

[0061] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0062] Example 5:

[0063] 42% Formula (I) Compound·Fipronil Wettable Powder (28:14)

[0064] Formula: 28% compound of formula (I), 14% fipronil, 4% naphthalene sulfonate formaldehyde condensate, 1.5% sodium lignosulfonate, 15% tea saponin, 2% sodium dodecyl sulfate, 0.5% citric acid, 5.5% starch, kaolin to make up the balance;

[0065] Preparation method: Same as in Example 4.

[0066] Example 6:

[0067] 38% Formula (I) Compound·Acetaminophen Wettable Powder (22:16)

[0068] Formula: 22% compound of formula (I), 16% acetamiprid, 5% naphthalene sulfonate formaldehyde condensate, 1.5% sodium lignosulfonate, 15% tea saponin, 2% sodium dodecyl sulfate, 0.45% citric acid, 6% starch, kaolin to make up the balance;

[0069] Preparation method: Same as in Example 4.

[0070] Example 7:

[0071] 42% Formula (I) Compound·Imidacloprid Water Dispersible Granules (30:12)

[0072] Formula: 30% of compound (I), 12% imidacloprid, 5% sodium lignosulfonate, 4% sodium polycarboxylate, 7.5% naphthalenesulfonate formaldehyde condensate, 2.5% sodium dodecyl sulfate, 4% sodium sulfate, and kaolin to make up the balance;

[0073] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added to it. After mixing, the mixture is pulverized by air jet and then an appropriate amount of water is added. The mixture is then kneaded, granulated, dried and sieved to obtain the water-dispersible granule product.

[0074] Example 8:

[0075] 50% Formula (I) compound·dinotefuran water-dispersible granules (35:15)

[0076] Formula: 35% compound of formula (I), 15% fipronil, 5% sodium lignosulfonate, 4% sodium polycarboxylate, 7.5% naphthalenesulfonate formaldehyde condensate, 2.5% sodium dodecyl sulfate, 3.5% sodium sulfate, and kaolin to make up the balance;

[0077] Preparation method: Same as in Example 7.

[0078] Example 9:

[0079] 46% Formula (I) Compound·Acetaminophen Water Dispersible Granules (32:14)

[0080] Formula: 32% compound of formula (I), 14% acetamiprid, 5% sodium lignosulfonate, 4% sodium polycarboxylate, 7.5% naphthalenesulfonate formaldehyde condensate, 2.5% sodium dodecyl sulfate, 15% silica, and kaolin to make up the balance;

[0081] Preparation method: Same as in Example 7.

[0082] Indoor bioactivity assay

[0083] Example Reference: Guidelines for Indoor Bioassay Testing of Pesticides

[0084] Part 6: Insect Immersion Method NY / T 1154.6-2006;

[0085] Part 7: Determination of the combined effects of mixtures NY / T 1154.7-2006;

[0086] Part 9: Spraying Method NY / T 1154.9-2008;

[0087] Part 14: Leaf dipping method NY / T 1154.14-2008.

[0088] Specific indoor toxicity tests:

[0089] Experimental method: Insect immersion method;

[0090] Test insects: healthy, uniformly grown and developed second-instar nymphs;

[0091] Reagent preparation: Dissolve the test reagent in acetone and prepare five series of concentrations using a 0.1% Tween 80 aqueous solution.

[0092] Experimental treatment: Second-instar larvae with consistent physiological condition and indoor rearing were selected. The test insects were immersed in the drug solution for 5 seconds using an insect dipping device. 15 insects were treated per treatment, and the treatment was repeated 4 times. The control group was treated with 0.1% Tween 80 aqueous solution. After treatment, the insects were transferred to a 9.0 cm diameter culture dish lined with filter paper for rearing. Fresh leaves were changed daily.

[0093] The treated test insects were raised in an artificial intelligence culture room at 25±1℃, with a light duration of L:D = 14h:10h and a relative humidity of 65%±5%.

[0094] Investigation method: The mortality of test insects was investigated 48 hours after the treatment with the agent. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when punctured. The total number of insects and the number of dead insects were recorded.

[0095] Data statistics and analysis:

[0096] Based on the survey data, the corrected mortality rates for each treatment were calculated. The calculations were performed using formulas (1) and (2), and the results were rounded to two decimal places.

[0097]

[0098] In the formula:

[0099] P – Mortality rate, expressed as a percentage (%);

[0100] K represents the number of dead insects, in heads;

[0101] N represents the total number of insects treated, in units of heads.

[0102]

[0103] In the formula:

[0104] P1 – Corrected mortality rate, in percentage (%);

[0105] P t —The mortality rate is expressed as a percentage (%).

[0106] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0107] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is >20%, the experiment needs to be repeated.

[0108] The data was processed using probability value analysis. The DPS statistical analysis system can be used to analyze the data and determine the toxicity regression line and LC. 50 The values, their 95% confidence limits, and correlation coefficients r are used to evaluate the activity of the test reagent on the biological sample.

[0109] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0110]

[0111] In the formula:

[0112] ATI – Actual Measured Toxicity Index of Mixtures;

[0113] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0114] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0115] TTI = TI A *P A +TI B *P B

[0116] In the formula:

[0117] TTI – Theoretical Toxicity Index of Mixtures;

[0118] TI A —A. Toxicity index of drug A;

[0119] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0120] TI B —Toxicity index of drug B;

[0121] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0122]

[0123] In the formula:

[0124] CTC – Cotoxicity Coefficient;

[0125] ATI – Actual Measured Toxicity Index of Mixtures;

[0126] TTI – Theoretical Toxicity Index of Mixtures.

[0127] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0128] Results of the combined toxicity test of mixed pairs with alfalfa aphids:

[0129] Table 1 shows the LC50 of imidacloprid against alfalfa aphids. 50 The LC50 of compound (I) against alfalfa aphids was 0.2614 mg / L. 50The concentration was 6.3730 mg / L. Among them, when the mass ratio of imidacloprid to compound of formula (I) was 1:52 to 55:1, the co-toxicity coefficient was greater than 80, showing an additive or synergistic effect; when the mass ratio of imidacloprid to compound of formula (I) was 1:45 to 30:1, the co-toxicity coefficient was greater than 120, showing a synergistic effect.

[0130] Table 1. Toxicity test results of imidacloprid and compound (I) on alfalfa aphids.

[0131]

[0132]

[0133] Table 2 shows the LC50 of fipronil against alfalfa aphids. 50 The LC50 of compound (I) against alfalfa aphids was 0.4187 mg / L. 50 The concentration was 6.4725 mg / L. Among them, when the mass ratio of fipronil to compound (I) was 1:64 to 60:1, the co-toxicity coefficient was greater than 80, showing an additive or synergistic effect; when the mass ratio of fipronil to compound (I) was 1:58 to 30:1, the co-toxicity coefficient was greater than 120, showing a synergistic effect.

[0134] Table 2. Toxicity test results of fipronil combined with compound (I) on alfalfa aphids.

[0135]

[0136] Table 3 shows the LC50 of acetamiprid against alfalfa aphids. 50 The LC50 of compound (I) against alfalfa aphids was 0.5001 mg / L. 50 The concentration was 6.6318 mg / L. Among them, the co-toxicity coefficient of acetamiprid with compound (I) at a mass ratio of 1:50 to 45:1 was greater than 80, showing an additive or synergistic effect; the co-toxicity coefficient of acetamiprid with compound (I) at a mass ratio of 1:50 to 35:1 was greater than 120, showing a synergistic effect.

[0137] Table 3. Toxicity test results of acetamiprid combined with compound (I) on alfalfa aphids.

[0138]

[0139] Field efficacy example 1

[0140] Experimental crops and targets: wheat (Luyuan 502), wheat aphid;

[0141] Experimental location: Experimental field in Wangji Township, Fucheng County, Hengshui City, Hebei Province;

[0142] Application equipment: 3WBS16 backpack electric sprayer;

[0143] Test date: May 15, 2023;

[0144] The field efficacy test design was based on GB / T 17980.79 2000 "Guidelines for Field Efficacy Tests of Pesticides (II) Insecticides for the Control of Wheat Aphids";

[0145] Experimental environment: The cultivation conditions in all experimental plots were uniform and the same level of fertilizer and water management was used;

[0146] Experimental treatment and reagents: 300 L / hm 2 Apply the pesticide evenly by spraying with water. The pesticide formulations are shown in the table below:

[0147] Table 4 Field Trial Design and Pesticide Dosage

[0148]

[0149]

[0150] Experimental method: Apply pesticide once during the peak period of aphid infestation.

[0151] Cell size and replication: Each treatment was replicated 4 times, with each cell size approximately 20m². 2 The buildings are arranged randomly, and protective barriers are set up around each residential area.

[0152] Survey Methods: A diagonal five-point sampling method was used, with 5 plants at each point. A total of 25 wheat plants were tagged in each plot, and the number of live insects on each plant was counted at fixed points. The initial insect population was assessed before pesticide application, and the number of remaining insects was assessed 1 day and 7 days after application.

[0153] Calculation formulas and data analysis:

[0154]

[0155] Data analysis: Microsoft Excel 2010 software was used to calculate the effectiveness of the treatment, and DPS statistical analysis software was used to perform differential analysis.

[0156] Results of efficacy trials:

[0157] The results in Table 5 show that, 1 day after application, 25% compound (I)·imidacloprid suspension (15:10) exhibited good rapid-acting properties, with the effective ingredient dosage being 30 g ai / hm. 2 Its efficacy was 90.37%; 7 days after administration, each mixed formulation also showed good sustained efficacy, with an efficacy of 95.48%, significantly higher than its control single agent.

[0158] Table 5 Field control effects of different pesticides

[0159]

[0160] Note: The above efficacy data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0161] Safety: During and after the trial period, the wheat grew normally and no obvious phytotoxicity was observed.

[0162] Field efficacy example 2

[0163] Experimental crops and targets: peach trees (nectarines), peach aphids;

[0164] Experimental location: Tianbao Peach Orchard Experimental Field, Pingyi County, Linyi City, Shandong Province;

[0165] Test date: March 10, 2024;

[0166] Experimental environment: The cultivation conditions in all experimental plots were uniform and the same level of fertilizer and water management was used;

[0167] Experimental treatment and pesticides: Before the peach trees bloom, a backpack electric sprayer was used to spray the peach trees with pesticides (working pressure: 0.3 MPa) until the pesticide solution formed water droplets on the leaves but did not drip.

[0168] The prescribed medications are shown in the table below:

[0169] Table 6 Field Trial Design and Pesticide Dosage

[0170]

[0171] Experimental method: Apply pesticide once during the peak period of aphid infestation.

[0172] Two peach trees were surveyed in each plot. Five affected shoots were fixed on each tree, oriented east, west, south, north, and center. The number of live aphids on the top 10 leaves of each shoot was surveyed. The initial aphid population was surveyed before the pesticide was applied, and the number of live aphids was surveyed 1 and 7 days after the pesticide was applied, for a total of 3 surveys.

[0173] Calculation formulas and data analysis:

[0174]

[0175] Data analysis: Microsoft Excel 2010 software was used to calculate the effectiveness of the treatment, and statistical analysis software was used to conduct differential analysis.

[0176] Experimental results:

[0177] Table 7 shows the results 1 day after application: 10, 20, and 30 g ai / hm of 20% compound (I)·fipronil suspension (14:6). 2The control efficacy against peach aphids was 82.55%, 87.43%, and 90.45%, respectively; 7 days after application, 20% compound (I)·fipronil suspension (14:6) at doses of 10, 20, and 30 g ai / hm 2 The control efficacy against peach aphids was 87.47%, 90.70%, and 95.87%, respectively. Among them, 20% compound (I)·fipronil suspension (14:6) 30g ai / hm 2 It has the highest control efficacy against peach aphids, significantly higher than its control single agent.

[0178] Table 7 Field control effects of different pesticides

[0179]

[0180] Note: The above efficacy data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0181] Safety: During the trial period and later observations, the peach trees grew normally and no obvious phytotoxicity was observed.

[0182] Field efficacy example 3

[0183] Experimental crops and targets: cotton (Zhongmian Institute 63), cotton aphid;

[0184] Test location: Jinxiang Science and Technology Demonstration Park, Jining City, Shandong Province;

[0185] Test date: June 25, 2023;

[0186] Experimental environment: The cultivation conditions in all experimental plots were uniform and the same level of fertilizer and water management was used;

[0187] Experimental treatment and pesticides: 3WBS16 backpack electric sprayer; uniform spraying with 15L / acre of water; pesticides used are shown in the table below:

[0188] Table 8 Field Trial Design and Pesticide Dosage

[0189]

[0190] Experimental method: Apply pesticide once during the peak period of aphid infestation.

[0191] Cell size and replication: Each treatment was replicated 4 times, with each cell size approximately 50m². 2 The buildings are arranged randomly, and protective barriers are set up around each residential area.

[0192] Safety survey: Visually inspect the cotton growth in each treated area on days 1, 7 and 14 after application, and compare it with the blank control area for any abnormalities.

[0193] Control efficacy survey: Five random sampling points were taken in each plot, with 10 cotton plants fixed at each point. The initial population of cotton aphids on each plant was investigated before spraying, and the residual number of aphids on the marked cotton plants in each plot was investigated 3 and 14 days after spraying.

[0194] Calculation formulas and data analysis:

[0195]

[0196] Data Analysis: The efficacy was calculated using Microsoft Excel 2010 software, and statistical analysis software was used for difference analysis. Field efficacy trial results:

[0197] Table 9 shows that all tested pesticides were effective in controlling cotton aphids. Three days after application, the 26% compound (I)·acetamiprid suspension (14:12) showed better rapid action, with effective dosages of 15, 22.5, and 30 g ai / hm². 2 The control efficacy against cotton aphids was 80.05%, 85.56%, and 90.40%, respectively. 14 days after application, the mixed formulations also showed good residual efficacy, with control efficacies of 86.67%, 90.30%, and 95.62%, respectively, which were significantly higher than their control single agents.

[0198] Table 9 Field control effects of different pesticides

[0199]

[0200] Note: The above efficacy data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0201] Safety: No phytotoxicity was found in any of the surveys after application, and there was no significant difference in cotton growth between the treatments and the control, indicating that each pesticide was safe for cotton at the dosages used in this experiment.

[0202] The pesticide compositions or formulations obtained by this invention exhibit significant preventative efficacy, demonstrating superior performance compared to single-agent formulations in delaying the development of resistance and prolonging pesticide retention. Furthermore, no phytotoxicity was observed in the experiments with the compounded pesticides, indicating that the enhanced synergistic insecticidal effect of the resulting pesticide compositions or formulations can reduce production and usage costs while ensuring crop safety.

[0203] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is any one of imidacloprid, dinotefuran, or acetamiprid, and active ingredient B is a compound of formula (I). The mass ratio of active ingredient A to active ingredient B is 1:64 to 60:

1.

2. The pesticide composition according to claim 1, characterized in that: The mass ratio of active ingredient A to active ingredient B is 1:58 to 35:

1.

3. The pesticide composition according to claim 1, characterized in that: The mass ratio of imidacloprid to compound (I) is 1:45 to 30:1, the mass ratio of dinotefuran to compound (I) is 1:58 to 30:1, and the mass ratio of acetamiprid to compound (I) is 1:50 to 35:

1. Preferably, the mass ratio of imidacloprid to compound (I) is 1:30 to 7:1, the mass ratio of dinotefuran to compound (I) is 1:30 to 10:1, and the mass ratio of acetamiprid to compound (I) is 1:35 to 5:

1.

4. The pesticide composition according to claim 1, characterized in that: Based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1% to 85%.

5. The pesticide composition according to claim 1, characterized in that: In addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, or carriers.

6. The pesticide composition according to claim 5, characterized in that: The pesticide composition is prepared into an agriculturally permissible formulation, wherein the formulation is selected from one or more of wettable powders, water-dispersible granules, water-in-oil emulsions, microemulsions, suspensions, suspension emulsions, and dispersible oil suspensions.

7. The application of the pesticide composition according to any one of claims 1-6 in the control of agricultural and forestry pests and sanitary pests.

8. The application according to claim 7, characterized in that: The agricultural and forestry pests and sanitary pests mentioned are pests belonging to the orders Hemiptera, Thysanoptera, and Lepidoptera.

9. The application according to claim 8, characterized in that: The hemiptera pests mentioned are aphids.

10. The application according to claim 1, characterized in that: The pesticide composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

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