Solid-phase gene chip molecular hybridization instrument

Abstract

The invention discloses a solid-phase gene chip molecular hybridization instrument which comprises a bottom plate, a heat cover, a swing driving assembly and a swing fulcrum assembly, and heating elements are arranged in the bottom plate and the heat cover; the control unit is used for controlling the temperature of the heating element and the swing driving assembly, the heating element emits heat at the same time, and the swing driving assembly drives the bottom plate, the heat cover and the molecular hybridization container to swing around the swing fulcrum assembly in a reciprocating mode at the same time. The molecular hybridization container has the advantages that on the premise that trace liquid does not lose, a variable-speed reciprocating motion field of the molecular hybridization container is constructed through a brand-new asymmetric swing mode with fluid mechanics gain, high-frequency shear force is induced on a solid-liquid interface, and diffusion limitation of the solid-liquid interface under a trace system is overcome; meanwhile, high-precision temperature control and anti-condensation effects are achieved through solid heat transfer on the upper side and the lower side, and the temperature non-uniformity and the fringe effect in traditional solid-phase hybridization are effectively solved.

Description

Technical Field

[0001] This invention belongs to the field of molecular biology technology, and in particular relates to a gene analysis instrument. Background Technology

[0002] Gene chips, also known as DNA microarrays, are a technology that uses a large number of oligonucleotide probes with known sequences to be fixed in an orderly manner on the surface of a solid carrier such as a silicon wafer, glass plate, or plastic sheet in a high-density microarray.

[0003] Molecular hybridization is a core step in gene chip technology. Its basic principle utilizes the denaturation and renaturation properties of nucleic acid molecules to allow fluorescently labeled DNA / RNA target sequences in a liquid phase to form double-stranded structures with probes on a solid-phase support through base complementarity. Qualitative or quantitative analysis of the target sequence is then achieved by detecting the fluorescence signal. This technology is widely used in disease diagnosis, drug screening, gene expression profiling, and pathogen detection. This step is generally performed within a molecular hybridization apparatus. Traditional static hybridization involves adding hybridization solutions to the chip surface, covering it with a coverslip, or placing it in a sealed chamber and then allowing it to react statically in a temperature-controlled incubator or water bath. Alternatively, the structure of the molecular hybridization apparatus, as disclosed in patent CN2485313Y, includes a temperature-controlled platform capable of heating and cooling, and a hybridization container frame capable of reciprocating motion.

[0004] Although the above methods have been applied, in practice, especially for solid-phase hybridization of micro-scale systems (μL level), some functional problems still need to be solved: First, molecular hybridization occurs at the solid-liquid interface (i.e., a two-dimensional interface). After the reaction begins, target molecules near the probe are rapidly bound, resulting in a "depleted layer" or "Nernst diffusion layer" with extremely low target molecule concentration near the solid surface. Target molecules deeper in the solution must rely on Brownian motion to cross this thick diffusion layer to reach the probe surface. Due to the small diffusion coefficient and slow kinetics of long DNA molecules, traditional static hybridization often takes more than 10 hours to reach equilibrium, resulting in extremely low efficiency. Existing hybridization containers with reciprocating motion have also failed to overcome the diffusion limitations at the solid-liquid interface in micro-scale systems.

[0005] Secondly, molecular hybridization is typically performed at high temperatures of 40℃-60℃. Tiny gaps can cause rapid liquid evaporation. Traditional air baths (thermostatic chambers) have low heat capacity and slow heat transfer, resulting in slow temperature recovery after opening. Furthermore, uneven air convection can easily lead to an "edge effect" on the chip, where the temperature differs between the center and edges, severely impacting hybridization specificity. Alternatively, another existing molecular hybridization container uses bottom heating, which causes liquid evaporation and condensation on the container lid, altering the hybridization solution concentration and similarly affecting hybridization performance. Summary of the Invention

[0006] Therefore, in order to solve the above problems, the present invention provides a high-efficiency, evaporation-proof, and temperature-uniform solid-phase gene chip molecular hybridization instrument.

[0007] This invention is achieved through the following technical solution: A solid-phase gene chip molecular hybridization instrument includes a device frame and an outer casing, and further includes: A base plate is placed inside the outer cover to fix the molecular hybridization container in place, and the upper surface of the base plate is in close contact with the bottom of the molecular hybridization container; A hot cap is hinged to one end of the base plate such that when it is flipped to the working position, the lower surface of the hot cap is in close contact with the top of the molecular hybridization container. Heating elements are provided inside both the base plate and the hot cover, and the bottom and top of the molecular hybridization container are located within the heating area of ​​the heating elements. A swing drive assembly and a swing fulcrum assembly are used to cooperate in driving the base plate, the hot cover and the molecular hybridization container to generate reciprocating swing simultaneously. The swing drive assembly includes an eccentric drive unit and a swing plate. The fixed end of the swing plate is fastened to the lower surface of the base plate, and its drive end receives the torque transmitted by the eccentric drive unit. The swing fulcrum assembly is offset from the swing drive assembly. It includes a fixed frame fixed to the equipment frame, and a bearing and a rotating shaft that are sleeved on each other. The outer ring of the bearing is fixed to the fixed frame, and its inner ring is fixed to the rotating shaft. And a control unit for controlling the temperature of the heating element and the swing drive assembly, wherein while the heating element located in the base plate and the hot cover is heating up, the swing drive assembly drives the base plate, the hot cover and the molecular hybridization container to swing back and forth around the rotating axis simultaneously.

[0008] Preferably, the eccentric drive unit includes a motor, a synchronous belt, a driving pulley, a driven pulley, an eccentric wheel, and an eccentric shaft. The driving pulley is fixed on the motor shaft of the motor, and the driving pulley drives the driven pulley to rotate through the synchronous belt. The eccentric wheel is coaxially fixed with the driven pulley, and the eccentric shaft is offset on the end face of the eccentric wheel.

[0009] Preferably, the driving end of the swing plate includes an open slot, and the eccentric shaft extends vertically into the open slot.

[0010] Preferably, the axis of the rotating shaft is offset from the axis of the eccentric wheel to cause the base plate, the hot cover and the molecular hybridization container to reciprocate at varying speeds.

[0011] Preferably, the eccentric drive unit further includes an adjustable tensioner.

[0012] Preferably, the eccentricity of the eccentric shaft on the eccentric wheel is adjustable.

[0013] Preferably, the swing fulcrum assembly further includes an outer peripheral limiting member, which includes an arched structure. The two free ends of the arched structure are respectively fixed to the fixing frame, and the arched part of the arched structure wraps around the outer ring of the bearing.

[0014] Preferably, a vibration motor is fixed on the base plate.

[0015] Preferably, the heating element is a PTC heating element, the base plate and the heat cover are both frame structures, the heating element is disposed in the frame-type receiving space of the base plate and the heat cover, and the base plate is provided with a limiting space for closely adhering to the bottom of the molecular hybridization container.

[0016] Preferably, the heating elements located within the base plate and the heating cover maintain the same temperature under the control of the control unit.

[0017] The beneficial effects of the technical solution of this invention are mainly reflected in: 1. Without losing trace amounts of liquid, a novel asymmetric oscillation mechanism with hydrodynamic gain is used to construct a variable-speed reciprocating motion field for the molecular hybridization container, inducing high-frequency shear force at the solid-liquid interface to overcome the diffusion limitation of the solid-liquid interface in trace systems; at the same time, inefficient air heat transfer is eliminated, and high-precision temperature control and anti-condensation effect are achieved by using solid heat transfer on both the upper and lower sides, effectively solving the temperature non-uniformity and edge effect in traditional solid-phase hybridization. 2. Based on the low-frequency large-amplitude oscillation driven by the eccentric wheel, a high-frequency vibration is superimposed to form a composite motion mode. The high-frequency vibration is used to break the surface tension of the liquid and the stagnant layer at the interface, while the low-frequency oscillation is used to replace the entire liquid, thereby significantly improving the hybridization efficiency and greatly shortening the hybridization time. 3. A dovetail groove is cut on the surface of the eccentric wheel, and the eccentricity of the eccentric shaft is adjusted by screws, thereby adjusting the oscillation amplitude of the low-frequency oscillation to adapt to hybrid liquids of different viscosities. Attached Figure Description

[0018] Figure 1 This is a three-dimensional schematic diagram of the solid-phase gene chip molecular hybridization instrument of the present invention; Figure 2 This is a three-dimensional schematic diagram of the solid-phase gene chip molecular hybridization instrument of the present invention, with part of the outer cover hidden; Figure 3 This is a front view of the solid-phase gene chip molecular hybridization instrument of the present invention; Figure 4 This is an axonometric sectional view of the solid-phase gene chip molecular hybridization instrument of the present invention, with the molecular hybridization container loaded at this time; Figure 5 yes Figure 4 Enlarged view of section A; Figure 6 This is a front view of the solid-phase gene chip molecular hybridization instrument of the present invention, with the molecular hybridization container loaded at this time; Figure 7 This is a schematic diagram of the adjustable tension wheel of the eccentric drive unit of the solid-phase gene chip molecular hybridization instrument of the present invention. Figure 8 This is a schematic diagram of the base plate of the solid-phase gene chip molecular hybridization instrument of the present invention in its initial position; Figure 9 This is a schematic diagram of the base plate of the solid-phase gene chip molecular hybridization instrument of the present invention in the right-handed position; Figure 10 This is a schematic diagram of the base plate of the solid-phase gene chip molecular hybridization instrument of the present invention in the left-hand swing position; Figure 11 This is a front view of another embodiment of the solid-phase gene chip molecular hybridization instrument of the present invention. Detailed Implementation

[0019] To make the objectives, advantages, and features of the present invention clearer and more detailed, the following non-limiting description of preferred embodiments will be illustrated and explained. These embodiments are merely typical examples of applying the technical solutions of the present invention; all technical solutions formed by equivalent substitutions or equivalent transformations fall within the scope of protection claimed by the present invention.

[0020] It should also be noted that in the description of the solution, the terms "center," "upper," "lower," "left," "right," "front," "rear," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These are used only for ease of description and simplification, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first" and "second" in this solution are used for descriptive purposes only and should not be construed as indicating or implying a ranking of importance, or implicitly specifying the number of technical features shown. Therefore, features defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0021] The solid-phase gene chip molecular hybridization instrument disclosed in this invention will be described below with reference to the accompanying drawings, such as... Figures 1-3As shown, the device includes a frame 1 and an outer cover 10. The frame 1 generally includes various interlocking beams and supports, forming a fixed working platform. In a preferred embodiment of the invention, the frame 1 is configured as a double-layer structure. The upper layer is configured as an electrical control room, mainly used to house the control unit 100, which serves as the electrical main control center of the device. The lower layer is configured as a chip molecular hybridization space, used to house the mechanical components for chip molecular hybridization. The outer cover 10 is used to enclose the frame 1, providing isolation and dust protection.

[0022] The lower equipment frame 1 inside the outer casing 10 is provided with a base plate 2 and a heat cover 3, combined with Figure 4 As shown, a molecular hybridization container 6 is disposed between the two. The molecular hybridization container 6 adopts a conventional structure, with the chip 60 and the hybridization solution located inside it. The molecular hybridization container 6 has a cuboid structure, including a planar top and bottom.

[0023] Reference Figure 3 and Figure 6 The base plate 2 and the heat cover 3 are connected at one end by a hinge 5. Specifically, a hinge platform 21 is provided on the outer side of the base plate 2, and the hinge 5 is located on it. Through this structure, the heat cover 3 can be flipped relative to the base plate 2, such as... Figure 6 As shown in the right-hand workstation, flipping open the heated cover 3 allows the molecular hybridization container 6 to be placed on the base plate 2, with the upper surface of the base plate 2 pressed against the bottom of the molecular hybridization container 6. Then, flipping the heated cover 3 back to its working position allows its lower surface to press against the top of the molecular hybridization container 6. In this way, the molecular hybridization container 6 is fixed between the base plate 2 and the heated cover 3. Of course, additional fixing mechanisms such as clips can be added between the base plate 2 and the heated cover 3.

[0024] Heating elements 4, which are PTC heating elements, are both provided within the base plate 2 and the hot cover 3. In this preferred embodiment, both the base plate 2 and the hot cover 3 are frame structures, and the heating elements 4 are disposed within the frame-like receiving space of the base plate 2 and the hot cover 3, avoiding the influence of environmental changes on the heating temperature. For example, the hot cover 3 includes a lower plate 31 and an upper plate 32, and the heating element 4 is located in the receiving space between the two. The base plate 2 can also adopt a similar frame structure. Furthermore, a support plate 33 is provided at the other end of the upper plate 32 opposite to the hinge 5. When the hot cover 3 is flipped to its working position, the support plate 33 is just supported on the base plate 2, providing overall support for the hot cover 3.

[0025] In this preferred embodiment, a limiting space is provided on the base plate 2 for closely adhering to the bottom of the molecular hybridization container 6. As is well known to those skilled in the art, this limiting space can match the outer contour of a single molecular hybridization container 6 or the outer contours of multiple molecular hybridization containers 6. Of course, it is also possible to provide a smooth surface on the base plate 2 to hold the molecular hybridization container 6, as long as the bottom and top of the molecular hybridization container 6 are both located within the heating area of ​​the heating element 4. This ensures that the temperature of each chamber in one or more molecular hybridization containers 6 is essentially uniform.

[0026] In this invention, solid-state heat transfer is used between the base plate 2, the heating cap 3, and the molecular hybridization container 6. Furthermore, the first heating element 41 located within the heating cap 3 and the second heating element 42 located on the base plate 2 maintain the same temperature under the control of the control unit 100, ensuring that the molecular hybridization container 6 and its internal environment remain within the set range. Simultaneously, solid-state heat transfer offers faster heat transfer speeds compared to gas-based methods, resulting in smaller and more uniform temperature overshoot during heating.

[0027] More preferably, in this invention, the temperatures of the heating elements 4 of the base plate 2 and the heating cover 3 can be controlled separately. By using dual probes to individually detect the temperatures of the first heating element 41 and the second heating element 42, redundant heating can be avoided even if a single module of the base plate 2 or the heating cover 3 fails, reducing the risk of experimental failure. In practical operation, considering heat loss, the actual temperature of the inner surface near the heating cover 3 may be slightly lower than that near the inner surface of the base plate 2, potentially causing liquid evaporation and condensation on the heating cover 3, thus altering the hybridization solution concentration. As is well known to those skilled in the art, temperature compensation can be used for control, i.e., controlling the heating temperature of the first heating element 41 to be 1-3°C higher than the heating temperature of the second heating element 42. This optimized control method, as an equivalent solution of this preferred embodiment, is also within the scope of protection of this invention.

[0028] The first heating element 41 and the second heating element 42 can be disposed within the frame structure of the base plate 2 and the heat cover 3 in various ways. They can be directly fixed or dynamically compensated and fixed using a flexible thermally conductive layer (such as a high thermal conductivity silicone pad). Other feasible fixing methods known to those skilled in the art, such as snap-fit, are alternatives to the present invention.

[0029] Another feature of this invention is that the base plate 2, the heating cap 3, and the molecular hybridization container 6 simultaneously reciprocate. (See attached diagram for specific structure.) Figures 4-7 It includes a swing drive assembly 8 and a swing fulcrum assembly 7, which are used to cooperate in driving.

[0030] The swing drive assembly 8 includes an eccentric drive unit and a swing plate 808. The eccentric drive unit includes a motor 801, a synchronous belt 802, a drive pulley 803, a driven pulley 804, an eccentric wheel 806, and an eccentric shaft 807. The drive pulley 803 is fixed to the motor shaft of the motor 801, thereby driving the drive pulley to rotate. The drive pulley 803 drives the driven pulley 804 to rotate via the synchronous belt 802. Figure 3 As shown, the eccentric wheel 806 and the driven pulley 804 are fixed to each other by a shaft 811 and a spline, and the eccentric shaft 807 is offset on the end face of the eccentric wheel 806.

[0031] The swing plate 808 includes a fixed end and a driving end. The fixed end of the swing plate 808 is fastened to the lower surface of the base plate 2. The driving end of the swing plate 808 is a fork mechanism 810, including an opening slot 809, into which the eccentric shaft 807 extends vertically.

[0032] The swing fulcrum assembly 7 is offset from the swing drive assembly 8. It includes a fixed frame 701, an outer peripheral limiting member 702, a bearing 703, and a rotating shaft 704, all fixed to the equipment frame 1. The bearing 703 is a deep groove ball bearing, which is sleeved with the rotating shaft 704. The outer peripheral limiting member 702 includes an arched structure, with its two free ends fixed to the fixed frame 701. The arched portion of the arched structure encloses the outer ring of the bearing 703. The inner ring of the bearing 703 is fixed to the rotating shaft 704. Specifically, a fastener 705 is fixed to the lower surface of the base plate 2, and the rotating shaft 704 extends into the fastener 705, with the two locked together by bolts 706.

[0033] The following is a brief description of the working process of this preferred embodiment. First, the molecular hybridization container 6 is placed and fixed between the base plate 2 and the heating cover 3. The control unit 100 controls the temperature of the heating element 4 to start heating, and simultaneously controls the start of the motor 801. The rotational power is transmitted to the eccentric wheel 806 through the synchronous belt 802, the driving pulley 803, and the driven pulley 804. The eccentric wheel 806 drives the eccentric shaft 807 to rotate eccentrically. Since the eccentric shaft 807 extends vertically into the opening groove 809, the eccentric shaft 807 transmits torque to the swing plate 808. Due to the sliding limit of the swing fulcrum assembly 7, the base plate 2, the heating cover 3, and the molecular hybridization container 6 can only swing back and forth around the rotating shaft 704 simultaneously. For details, please refer to [reference needed]. Figure 8 , Figure 9 , Figure 10The demonstration of reciprocating oscillation. This reciprocating oscillation form constructs a variable-speed reciprocating motion field inside the molecular hybridization container 6, and uses the abrupt change of inertial force to generate chaotic convection, inducing high-frequency shear force at the solid-liquid interface, overcoming the diffusion limitation of the solid-liquid interface in the micro-system, and effectively breaking the Nernst diffusion layer at the solid-liquid interface.

[0034] Furthermore, such as Figure 5 As shown, the axis m of the rotating shaft 704 and the axis n of the eccentric wheel 806 can also be offset by a distance L, so that the base plate 2, the heated cover 3, and the molecular hybridization container 6 can produce variable-speed reciprocating oscillations. This is because, due to the existence of geometric offset, the rotation angle of the eccentric shaft 807 in the first half of the cycle (corresponding to the outward oscillation) is greater than the rotation angle in the second half of the cycle (corresponding to the return oscillation) under the action of torque, forming a slow inward (mixing) to a rapid return (turbulence) effect. At the instant of the "rapid return", the angular acceleration α of the hybridization solution increases sharply, generating a greater shear force, instantly destroying the Nernst diffusion layer at the solid-liquid interface, forcing fresh target sequence molecules to contact the probe surface. This non-periodic velocity change prevents the formation of stable streamlines and achieves a macroscopic effect similar to "stirring".

[0035] Based on this, those skilled in the art can foresee that a dovetail groove is provided on the eccentric wheel 806, and the eccentricity of the eccentric shaft 807 is adjusted by screws, thereby adjusting the amplitude of the swing to adapt to hybridization solutions of different viscosities. Alternatively, according to actual needs, a counterweight can be provided on the eccentric wheel 806 to counteract centrifugal vibration and make the motor load more stable.

[0036] This invention employs an eccentric mechanism, enabling the motor to rotate in only one direction to achieve the reciprocating oscillation of the base plate 2, the hot cover 3, and the molecular hybridization container 6. This avoids the instability caused by an excessively heavy oscillation mechanism and the motor's back-and-forth reversal. Thus, the unidirectional rotation of the motor means that a high-torque, unidirectional asynchronous motor can be selected, eliminating the need for a costly and low-torque stepper motor. Simultaneously, the drive pulley 803 is locked to the motor shaft of the motor 801 by screws 815. The unidirectional rotation of the motor also tightens the drive pulley 803 over time, preventing it from slipping off. Furthermore, the synchronous belt 802 remains taut, ensuring that the oscillation is not disrupted due to loosening of the synchronous belt 802 after prolonged operation.

[0037] Of course, although unidirectional rotation can tighten the belt, the belt will inevitably stretch over long-term operation. Therefore, in another embodiment of the present invention, the eccentric drive unit further includes an adjustable tensioner 805. See details below. Figure 7As shown, a fixing block 812 is provided on the equipment frame 1, and a row of threaded holes is formed on its upper surface. A sliding block 813 is provided above the fixing block 812. The sliding block 813 has a waist-shaped groove 814. By selectively screwing a bolt into any one of the threaded holes, the position change and fixation between the sliding block 813 and the fixing block 812 can be realized. An adjustable tensioning wheel 805 is provided at one end of the sliding block 813. When the sliding block 813 changes position relative to the fixing block 812, the tension force applied to the synchronous belt 802 by the adjustable tensioning wheel 805 also changes.

[0038] like Figure 11 Another embodiment of the present invention shown differs from the preferred embodiment in that a vibration motor 9 is fixed on the base plate 2. Based on the low-frequency large-amplitude oscillation driven by the eccentric wheel of the present invention, a high-frequency micro-vibration is superimposed. This high-frequency micro-vibration breaks the surface tension of the hybridization solution and the stagnant layer at the solid-liquid interface, while the low-frequency oscillation displaces the entire liquid, thereby significantly improving hybridization efficiency and shortening hybridization time.

[0039] This invention has many other embodiments, and all technical solutions formed by equivalent transformation or equivalent transformation fall within the protection scope of this invention.

Claims

1. A solid-phase gene chip molecular hybridization instrument, comprising an equipment frame (1) and an outer casing (10), characterized in that: Also includes: A base plate (2) is placed inside the outer cover (10) for fixing the molecular hybridization container (6). The upper surface of the base plate (2) is in close contact with the bottom of the molecular hybridization container (6). A hot cover (3) is connected to one end of the base plate (2) by a hinge (5) such that when it is flipped to the working position, the lower surface of the hot cover (3) is in close contact with the top of the molecular hybridization container (6); Heating elements (4) are provided in both the base plate (2) and the hot cover (3), and the bottom and top of the molecular hybridization container (6) are located within the heating area of ​​the heating elements (4); A swing drive assembly (8) and a swing fulcrum assembly (7) are used to cooperate in driving the base plate (2), the hot cover (3) and the molecular hybridization container (6) to generate reciprocating swing simultaneously; The swing drive assembly (8) includes an eccentric drive unit and a swing plate (808). The fixed end of the swing plate (808) is fastened to the lower surface of the base plate (2), and its drive end receives the torque transmitted by the eccentric drive unit. The swing fulcrum assembly (7) is offset from the swing drive assembly (8). It includes a fixed frame (701) fixed on the equipment frame (1), and a bearing (703) and a rotating shaft (704) that are sleeved together. The outer ring of the bearing (703) is fixed to the fixed frame (701), and its inner ring is fixed to the rotating shaft (704). And a control unit (100) for controlling the temperature of the heating element (4) and the swing drive assembly (8). While the heating element (4) located in the base plate (2) and the hot cover (3) is heating up, the swing drive assembly (8) drives the base plate (2), the hot cover (3) and the molecular hybridization container (6) to swing back and forth around the rotating shaft (704) at the same time.

2. The solid-phase gene chip molecular hybridization instrument according to claim 1, characterized in that: The eccentric drive unit includes a motor (801), a synchronous belt (802), a drive pulley (803), a driven pulley (804), an eccentric wheel (806), and an eccentric shaft (807). The drive pulley (803) is fixed on the motor shaft of the motor (801). The drive pulley (803) drives the driven pulley (804) to rotate through the synchronous belt (802). The eccentric wheel (806) is coaxially fixed with the driven pulley (804). The eccentric shaft (807) is offset on the end face of the eccentric wheel (806).

3. The solid-phase gene chip molecular hybridization instrument according to claim 2, characterized in that: The drive end of the swing plate (808) includes an opening slot (809), and the eccentric shaft (807) extends vertically into the opening slot (809).

4. The solid-phase gene chip molecular hybridization instrument according to claim 3, characterized in that: The axis (m) of the rotating shaft (704) is offset from the axis (n) of the eccentric wheel (806) so that the base plate (2), the heat cover (3) and the molecular hybridization container (6) can reciprocate at varying speeds.

5. The solid-phase gene chip molecular hybridization instrument according to claim 1, characterized in that: The eccentric drive unit also includes an adjustable tensioner (805).

6. The solid-phase gene chip molecular hybridization instrument according to claim 2, characterized in that: The eccentricity of the eccentric shaft (807) on the eccentric wheel (806) is adjustable.

7. The solid-phase gene chip molecular hybridization instrument according to claim 1, characterized in that: The swing fulcrum assembly (7) also includes an outer peripheral limiting member (702), which includes an arched structure. The two free ends of the arched structure are respectively fixed to the fixing frame (701), and the arched part of the arched structure wraps around the outer ring of the bearing (703).

8. The solid-phase gene chip molecular hybridization instrument according to any one of claims 1-7, characterized in that: A vibration motor (9) is fixed on the base plate (2).

9. The solid-phase gene chip molecular hybridization instrument according to claim 1, characterized in that: The heating element (4) is a PTC heating element. The base plate (2) and the heat cover (3) are both frame structures. The heating element (4) is set in the frame-type receiving space of the base plate (2) and the heat cover (3). The base plate (2) is provided with a limiting space for closely adhering to the bottom of the molecular hybridization container (6).

10. The solid-phase gene chip molecular hybridization instrument according to claim 1, characterized in that: The heating element (4) located in the base plate (2) and the heat cover (3) is kept at the same temperature under the control of the control unit (100).

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