Vacuum irradiation device for living cells and experimental method

Abstract

The invention relates to the technical field of biological experiment equipment, in particular to a living cell vacuum irradiation device and an experiment method. The living cell vacuum irradiation device comprises a driving mechanism which is provided with a first flange and a telescopic rod; the vacuum chamber is provided with a first port and a second port; the living cell carrier is used for keeping cell activity; wherein the first flange is connected with the first port, the first end of the telescopic rod extends into the vacuum chamber from the first port, and the living cell carrier is mounted at the first end of the telescopic rod; the second port is connected with a proton accelerator, and the living cell carrier is driven by the telescopic rod to adjust the position relative to the second port so as to receive ion irradiation. In the invention, by optimizing the structure of the irradiation device, the living cell carrier can be placed in the vacuum chamber, so that the ion beam directly irradiates the living cells, and the irradiation of the living cells can be implemented by using a low-energy accelerator of which the proton energy is more than 0.32 MeV.

Description

Technical Field

[0001] This invention relates to the field of biological experimental equipment technology, and in particular to a live cell vacuum irradiation device and experimental method. Background Technology

[0002] Cell models are among the most widely used models in space life sciences, and cell culture is the foundation of cell experiments. However, limitations exist due to the differences between the experimental environment of ion irradiation and the ideal cell culture environment. For example, lower carbon dioxide levels under experimental conditions can lead to increased pH, and temperature changes can affect cell metabolism. Inevitably, cells undergo structural and functional changes during experiments, even significantly shortening their survival time. Therefore, to eliminate these adverse effects, it is essential to control the environmental conditions during irradiation experiments to simulate the in vivo survival environment as closely as possible.

[0003] Currently, with the rapid development of manned spaceflight, lunar and Martian landings, and proton and heavy ion therapy for cancer, radiation medicine is widely used, leading to a surge in demand for cell irradiation experiments. When irradiating living cells with ions, placing the cell culture dish directly in a vacuum environment will cause cell dehydration and death. Therefore, in current technology, to maintain the survival environment of living cells, the cell culture dish is usually placed in an atmospheric environment, and then the ion beam is passed through a titanium window that separates the vacuum environment from the atmospheric environment to achieve ion irradiation.

[0004] When the ion beam travels from the vacuum environment of a proton accelerator to the atmosphere, it encounters a titanium window that causes energy attenuation. Furthermore, to withstand the pressure difference between the vacuum and atmosphere, the titanium window is typically thicker than 30 micrometers. Calculations show that for a proton to pass through a 30-micrometer titanium window, its energy must be at least 11.9 MeV. This means that many low-energy proton accelerators (approximately 75% of research-grade proton accelerators have energies concentrated in the 1-10 MeV range) cannot be directly used for live-cell irradiation experiments.

[0005] In view of this, there is an urgent need to design an irradiation device that can use a low-energy proton accelerator to conduct live cell irradiation experiments. Summary of the Invention

[0006] This invention aims to solve the technical problems existing in related technologies. To this end, this invention proposes a live cell vacuum irradiation device and experimental method to achieve the purpose of ion irradiation of live cells in a vacuum environment while maintaining cell viability.

[0007] In a first aspect, the present invention provides a live cell vacuum irradiation device, comprising: The drive mechanism is equipped with a first flange and a telescopic rod; The vacuum chamber is equipped with a first port and a second port; Live cell carriers are used to maintain cell viability; Wherein, the first flange is connected to the first port, the first end of the telescopic rod extends into the vacuum chamber from the first port, and the live cell carrier is installed at the first end of the telescopic rod; The second port is connected to a proton accelerator, and the live cell carrier is adjusted relative to the second port by the telescopic rod to receive ion irradiation.

[0008] According to the present invention, a vacuum irradiation device for live cells is provided, wherein the live cell carrier comprises: A seat body, wherein a cavity is provided on one side of the seat body; A fixing plate, wherein the fixing plate is provided with beam interfaces extending through both sides thereon; A metal composite film is sandwiched between the base and the fixing plate, and seals the opening of the cavity; The cavity is filled with a culture medium, and the beam interface is aligned with the opening of the cavity.

[0009] According to the present invention, a vacuum irradiation device for live cells is provided, wherein a sink is provided on one side of the fixing plate, the central axis of the sink coincides with the central axis of the beam interface, and the metal composite film is embedded in the sink.

[0010] According to the present invention, a vacuum irradiation device for live cells is provided, wherein a boss extending outward from the seat body is provided on one side of the seat body, the cavity is provided inside the boss, and the opening of the cavity is provided on the end face of the boss.

[0011] According to the present invention, a live cell vacuum irradiation device is provided, wherein the live cell carrier further includes an annular sealing member, and the end face of the boss is provided with a sealing groove surrounding the opening of the cavity, the sealing member being embedded in the sealing groove and abutting against the metal composite membrane.

[0012] According to the present invention, a vacuum irradiation device for live cells is provided, wherein the first flange is installed at one end of the drive mechanism, and the telescopic rod passes through the first flange; The drive mechanism also includes a first motor, which is connected to the second end of the telescopic rod.

[0013] According to the present invention, a vacuum irradiation device for live cells is provided, wherein the driving mechanism further includes: A second motor, the output shaft of which is parallel to the telescopic rod; The first gear is fitted onto the output shaft of the second motor; The second gear is fitted onto the telescopic rod and meshes with the first gear.

[0014] According to the present invention, a live cell vacuum irradiation device is provided, wherein the end face of the boss is further provided with a threaded hole, and at least four of the threaded holes are evenly distributed on the outer periphery of the cavity opening, and the fixing plate is provided with a through hole adapted to the threaded hole for threading bolts.

[0015] According to the present invention, a vacuum irradiation device for living cells is provided, wherein the metal composite membrane is made of Havar alloy.

[0016] Secondly, the present invention also provides an experimental method for performing an ion irradiation step using any of the above-described live cell vacuum irradiation devices, wherein the live cell carrier comprises: A seat body, wherein a cavity is provided on one side of the seat body; A fixing plate, wherein the fixing plate is provided with beam interfaces extending through both sides thereon; A metal composite film is sandwiched between the base and the fixing plate, and seals the opening of the cavity; The experimental steps include: filling the cavity with culture medium; HeLa cells were inoculated on the side of the metal composite membrane that was in contact with the culture medium. HeLa cells were irradiated with ions, and changes in cell morphology were recorded.

[0017] The above-described one or more technical solutions of this invention have at least one of the following technical effects: By optimizing the structure of the irradiation device, a live cell carrier can be placed in a vacuum chamber. By separating the vacuum environment from the atmospheric environment through the live cell carrier, the ion beam can directly irradiate the cells, avoiding the attenuation effect of the titanium window on the ion beam. This allows live cell irradiation to be carried out using a low-energy proton accelerator with a proton energy above 0.32 MeV, filling the gap that low-energy proton accelerators with proton energies between 0.32 MeV and 11.9 MeV cannot perform live cell irradiation experiments. This meets the cell-level experimental needs of scenarios such as aerospace radiation protection, cancer radiotherapy mechanism research, and radiation biology.

[0018] In addition to the technical problems solved by the present invention, the technical features of the technical solutions constituted by the present invention, and the advantages brought about by the technical features of these technical solutions as described above, other technical features of the present invention and the advantages brought about by these technical features will be further explained in conjunction with the accompanying drawings, or will be learned through the practice of the present invention. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or related technologies, the drawings used in the description of the embodiments or related technologies will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a three-dimensional structural diagram of the vacuum irradiation device for live cells provided in an embodiment of the present invention.

[0021] Figure 2 This is a three-dimensional schematic diagram of a live cell carrier installed on a drive mechanism, as provided in an embodiment of the present invention.

[0022] Figure 3 A front view of a live cell carrier installed on a drive mechanism, as provided in an embodiment of the present invention.

[0023] Figure 4 This is a front view of a live cell carrier provided in an embodiment of the present invention.

[0024] Figure 5 for Figure 4 Cross-sectional view of section AA of the live cell carrier.

[0025] Figure 6 for Figure 5 Enlarged schematic diagram of partial view B in the middle.

[0026] Figure 7 for Figure 4 Three-dimensional cross-sectional view of the live cell carrier along section AA.

[0027] Figure 8 Microscopic images obtained after irradiating cells with 4 Gy, 6 Gy and 8 Gy respectively, as provided in embodiments of the present invention.

[0028] Figure label: 100. Drive mechanism; 110. First flange; 120. Telescopic rod; 130. First motor; 140. Second motor; 150. First gear; 160. Second gear; 200. Vacuum chamber; 210. First port; 220. Second port; 300. Live cell carrier; 310. Base; 311. Cavity; 312. Boss; 313. Sealing groove; 320. Fixing plate; 321. Beam interface; 322. Settling tank; 330. Metal composite membrane; 340. Seal. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0030] In an embodiment of the present invention, a vacuum irradiation device for living cells is described.

[0031] like Figures 1 to 3 As shown, the live cell vacuum irradiation device mainly includes a drive mechanism 100, a vacuum chamber 200, and a live cell carrier 300. The drive mechanism 100 is equipped with a first flange 110 and a telescopic rod 120. The first flange 110 is installed at one end of the drive mechanism 100. The telescopic rod is movably inserted through the first flange 110.

[0032] The vacuum chamber 200 is a shell structure with an internal cavity. The shell structure is provided with a first port 210 and a second port 220.

[0033] The live cell carrier 300 includes a cavity 311 and a metal composite membrane 330. The cavity 311 is filled with culture medium. The metal composite membrane 330 seals the opening of the cavity 311, isolating it from a vacuum environment. Simultaneously, when the ion beam irradiates the live cells in the culture medium from the metal composite membrane 330, it must pass through the metal composite membrane 330, the culture medium, and the live cells to reach the cell nucleus and chromosomes. The thickness of the metal composite membrane 330 is approximately 5 μm, and the thickness of the live cells is approximately 5 μm to 20 μm. To reduce the proton energy required for irradiation experiments, it is preferable to use a cell adhesion method for sealed culture within the live cell carrier 300.

[0034] In this way, by inoculating live cells on the side of the metal composite membrane 330 that is in contact with the culture medium so that the live cells face the opening of the cavity 311, the required proton energy can be reduced by shortening the irradiation range, and the cells can be kept active when the live cell carrier 300 is in a vacuum environment.

[0035] The first flange 110 is connected to the first port 210. The first end of the telescopic rod 120 extends into the vacuum chamber 200 from the first port 210. The live cell carrier 300 is mounted on the first end of the telescopic rod 120. Connecting the second port 220 to the proton accelerator allows the ion beam to directly enter the interior of the vacuum chamber 200 through the second port 220.

[0036] Thus, the live cell carrier 300 is adjusted relative to the second port 220 under the action of the telescopic rod 120, and the ion beam generated by the proton accelerator can directly irradiate the cells in the live cell carrier 300 through the second port 220.

[0037] Furthermore, the live cell carrier 300 mainly includes a base 310, a fixing plate 320, and a metal composite membrane 330.

[0038] The base 310 has a cavity 311 on one side. The fixing plate 320 has a beam interface 321 that extends through both sides of it.

[0039] The metal composite film 330 is sandwiched between the base 310 and the fixing plate 320. Furthermore, the metal composite film 330 seals the opening of the recess 311.

[0040] The beam interface 321 is aligned with the opening of the cavity 311. HeLa cells are inoculated on the side of the metal composite membrane 330 that is in contact with the culture medium.

[0041] Furthermore, a recessed groove 322 is provided on one side of the fixing plate 320. The central axis of the recessed groove 322 coincides with the central axis of the beam interface 321. The metal composite membrane 330 is embedded in the recessed groove 322.

[0042] The vacuum chamber 200 also includes a rapid pressure relief valve, which is used to rapidly increase the gas pressure inside the vacuum chamber to atmospheric pressure, so as to quickly remove cells for cell parameter detection after ion irradiation.

[0043] The live cell vacuum irradiation device in this embodiment can place the live cell carrier 300 inside the vacuum chamber 200. By separating the vacuum environment from the atmospheric environment through the live cell carrier 300, the ion beam can directly irradiate the cells, avoiding the attenuation effect of the titanium window on the ion beam. This allows live cell irradiation to be carried out using a low-energy proton accelerator with a proton energy of 0.32 MeV or higher, filling the gap that low-energy proton accelerators with proton energies between 0.32 MeV and 11.9 MeV cannot perform live cell irradiation experiments. This meets the cell-level experimental needs of scenarios such as aerospace radiation protection, cancer radiotherapy mechanism research, and radiation biology.

[0044] Based on the above embodiments, another embodiment of the present invention introduces a live cell vacuum irradiation device.

[0045] like Figures 4 to 7As shown, a boss 312 is also provided on one side of the seat 310. The boss 312 extends outward from the seat 310. The cavity 311 is disposed inside the boss 312. The opening of the cavity 311 is disposed on the end face of the boss 312.

[0046] In addition, to improve the sealing between the metal composite membrane 330 and the boss 312, the live cell carrier 300 also includes an annular seal 340.

[0047] The end face of the boss 312 is provided with a sealing groove 313. The sealing groove 313 is arranged around the opening of the cavity 311. The sealing member 340 is embedded in the sealing groove 313. Furthermore, the sealing member 340 abuts against the metal composite film 330.

[0048] Based on the above embodiments, another embodiment of the present invention introduces a live cell vacuum irradiation device.

[0049] The first flange 110 is installed at one end of the drive mechanism 100. The telescopic rod 120 passes through the first flange 110.

[0050] The drive mechanism 100 also includes a first motor 130. The first motor 130 is mounted at the other end of the drive mechanism 100. The first motor 130 is connected to the second end of the telescopic rod 120.

[0051] Furthermore, in order to enable the drive mechanism 100 to rotate the live cell carrier 300 within the vacuum chamber 200, correct the relative position of the live cell carrier 300 and the second port 220, and allow the ion beam to better irradiate the cells, the drive mechanism 100 also includes a second motor 140, a first gear 150, and a second gear 160.

[0052] Specifically, the second motor 140 is mounted at the other end of the drive mechanism 100. The output shaft of the second motor 140 is parallel to the telescopic rod 120. The first gear 150 is sleeved on the output shaft of the second motor 140.

[0053] The second gear 160 is sleeved on the telescopic rod 120. Furthermore, the second gear 160 meshes with the first gear 150, realizing the transmission connection between the second motor 140 and the telescopic rod 120, which can drive the telescopic rod 120 to rotate.

[0054] Furthermore, to improve the sealing between the metal composite membrane 330 and the boss 312, threaded holes are also provided on the end face of the boss 312. At least four threaded holes are evenly distributed on the outer periphery of the opening of the cavity 311, and the fixing plate 320 is provided with through holes that are adapted to the threaded holes for threading bolts.

[0055] like Figure 4 As shown, the six threaded holes are evenly distributed on the same circumference, and the fixing plate 320 is provided with through holes that are adapted to the threaded holes, thereby ensuring that the compressive force applied by the fixing plate 320 to the metal composite film 330 is evenly distributed.

[0056] Preferably, the metal composite film 330 is made of Havar alloy.

[0057] Furthermore, in another embodiment of the present invention, an experimental method is also provided. This experimental method employs the live-cell vacuum irradiation apparatus described in any of the above embodiments to perform the ion irradiation step.

[0058] The live cell vacuum irradiation device mainly includes a drive mechanism 100, a vacuum chamber 200, and a live cell carrier 300. The drive mechanism 100 is equipped with a first flange 110 and a telescopic rod 120. The first flange 110 is installed at one end of the drive mechanism 100. The telescopic rod is movably inserted through the first flange 110.

[0059] The vacuum chamber 200 is a shell structure with an internal cavity. The shell structure is provided with a first port 210 and a second port 220.

[0060] The live cell carrier 300 includes a cavity 311 and a metal composite membrane 330. The cavity 311 is filled with culture medium. The metal composite membrane 330 seals the opening of the cavity 311, thus sealing the cavity 311 to isolate it from a vacuum environment.

[0061] The experimental steps include: S1, filling the cavity 311 with culture medium; S2, inoculating HeLa cells on the side of the metal composite membrane 330 that is in contact with the culture medium; S3, irradiating the HeLa cells with ions and recording changes in cell morphology.

[0062] Specifically, DMEM culture medium was infused into the cavity 311 of the live cell carrier 300. HeLa cells (density 2 × 10⁻⁶) were seeded inside the metal composite membrane 330. 4 / cm²). The live cell carrier 300 is mounted onto the telescopic tube of the drive mechanism 100, and then the vacuum chamber 200 is evacuated until the internal vacuum level of the chamber 200 reaches 5×10⁻⁻⁻⁶. 4 After Pa, the proton accelerator is then controlled to output 0.8 MeV of alpha protons (flux rate 1 × 10⁻⁶). 8 Cells were irradiated with 4 Gy, 6 Gy, and 8 Gy, respectively, at concentrations of ions / cm²·s. Changes in cell morphology were recorded simultaneously.

[0063] After irradiation, the cells were stained with fluorescence and the irradiation effect was observed.

[0064] The cells were divided into two groups and incubated in an incubator (37℃, 5% CO2) for 1 h and 2 h, respectively. Afterward, the cell culture medium was aspirated, the cells were washed twice with PBS, and then fixed with 2 ml of pre-cooled 4% paraformaldehyde fixative for 20 min.

[0065] Wash the slides three times with PBS, 5 min each time; permeabilize the cells by adding 1 ml of pre-chilled 0.5% Triton-100 and incubating on ice for 10-15 min.

[0066] At room temperature, add 2 ml of 5% BSA blocking solution and incubate for 1 hour. Wash once with TBST.

[0067] Then, anti-incubation was performed: 0.5 ml of r-H2AX antibody diluted 1500 times was added, and the mixture was incubated overnight at 4°C; the mixture was washed three times with TBST for 5-10 minutes each time.

[0068] Then perform secondary antibody incubation: Add 0.5 ml of Affinipure Goat Anti-Rabbit IgG (H+L) secondary antibody and incubate in the dark for 1 hour. Wash twice with TBST, 5-10 min each time.

[0069] Add 2 drops of DAPI staining solution containing mounting medium and incubate at room temperature for 5 minutes; finally, observe using a laser confocal microscope. The data below are obtained.

[0070] Based on the above data, it can be seen that at radiation doses of 4 Gy, 6 Gy, and 8 Gy, the higher the dose, the more severe the DNA damage. Furthermore, the repair effect is better after 2 hours of irradiation compared to 1 hour, as shown in the observed images. Figure 8 As shown.

[0071] In the description of the embodiments of the present invention, it should be noted that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of the present invention. In addition, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0072] In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention based on the specific circumstances.

[0073] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0074] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms are not limited to the same embodiments or examples. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A vacuum irradiation device for living cells, characterized in that, include: The drive mechanism (100) is provided with a first flange (110) and a telescopic rod (120). The vacuum chamber (200) is provided with a first port (210) and a second port (220); Live cell carrier (300) for maintaining cell viability; Wherein, the first flange (110) is connected to the first port (210), the first end of the telescopic rod (120) extends into the vacuum chamber (200) from the first port (210), and the live cell carrier (300) is installed at the first end of the telescopic rod (120); The second port (220) is connected to the proton accelerator, and the live cell carrier (300) is adjusted relative to the second port (220) by the telescopic rod (120) to receive ion irradiation.

2. The live cell vacuum irradiation device according to claim 1, characterized in that, The live cell carrier (300) includes: A seat (310) has a cavity (311) on one side. A fixing plate (320) is provided with a beam interface (321) that extends through both sides of the fixing plate (320). A metal composite film (330) is sandwiched between the base (310) and the fixing plate (320) and seals the opening of the cavity (311); The cavity (311) is filled with culture medium, and the beam interface (321) is aligned with the opening of the cavity (311).

3. The live cell vacuum irradiation device according to claim 2, characterized in that, A groove (322) is provided on one side of the fixing plate (320), the central axis of the groove (322) coincides with the central axis of the beam interface (321), and the metal composite film (330) is embedded in the groove (322).

4. The live cell vacuum irradiation device according to claim 3, characterized in that, A boss (312) extending outward from the seat (310) is also provided on one side of the seat (310), and a cavity (311) is provided inside the boss (312), with the opening of the cavity (311) provided on the end face of the boss (312).

5. The live cell vacuum irradiation device according to claim 4, characterized in that, The live cell carrier (300) also includes an annular seal (340), and the end face of the boss (312) is provided with a sealing groove (313) surrounding the opening of the cavity (311). The seal (340) is embedded in the sealing groove (313) and abuts against the metal composite membrane (330).

6. The live cell vacuum irradiation device according to any one of claims 1 to 5, characterized in that, The first flange (110) is installed at one end of the drive mechanism (100), and the telescopic rod (120) passes through the first flange (110). The drive mechanism (100) further includes a first motor (130), which is connected to the second end of the telescopic rod (120) in a transmission connection.

7. The live cell vacuum irradiation device according to claim 6, characterized in that, The drive mechanism (100) further includes: The output shaft of the second motor (140) is parallel to the telescopic rod (120); The first gear (150) is sleeved on the output shaft of the second motor (140); The second gear (160) is sleeved on the telescopic rod (120) and meshes with the first gear (150).

8. The live cell vacuum irradiation device according to claim 4 or 5, characterized in that, The end face of the boss (312) is also provided with threaded holes, and at least four of the threaded holes are evenly distributed on the outer periphery of the opening of the cavity (311). The fixing plate (320) is provided with through holes that are adapted to the threaded holes for threading bolts.

9. The live cell vacuum irradiation device according to claim 2, characterized in that, The metal composite film (330) is made of Havar alloy.

10. An experimental method, characterized in that, The ion irradiation step is performed using a live cell vacuum irradiation device as described in any one of claims 1 to 9, wherein the live cell carrier (300) comprises: A seat (310) has a cavity (311) on one side. A fixing plate (320) is provided with a beam interface (321) that extends through both sides of the fixing plate (320). A metal composite film (330) is sandwiched between the base (310) and the fixing plate (320) and seals the opening of the cavity (311); The experimental steps include: filling the cavity (311) with culture medium; HeLa cells were inoculated on the side of the metal composite membrane (330) that was in contact with the culture medium. HeLa cells were irradiated with ions, and changes in cell morphology were recorded.

Images