A culture device and use method of a saline-alkali soil improvement microbial agent

Abstract

This invention relates to the field of microbial inoculant cultivation technology, specifically a cultivation device and method for a saline-alkali land improvement inoculant, comprising: a nutrient solution tank with a lid on top; a replenishment component including a replenishment rod, the first end of which is located on the outside of the nutrient solution tank, and the second end of which passes through the lid and extends to the bottom of the nutrient solution tank; the replenishment rod having a first replenishment chamber and a second replenishment chamber inside, with a first electrically controlled valve between the first and second replenishment chambers; and an inoculant cultivation component located inside the nutrient solution tank and connected to the output end of a positioning component. In use, external nutrient solution can directly enter the nutrient solution tank through the replenishment rod, allowing for nutrient replenishment without removing the lid or the inoculant cultivation component. The nutrient solution always enters the inoculant cultivation component and mixes thoroughly with the inoculant.

Description

Technical Field

[0001] This invention relates to the field of microbial inoculant cultivation technology, specifically to a cultivation device and method for a saline-alkali land improvement inoculant. Background Technology

[0002] Saline-alkali land improvement microbial agents are biological preparations made using salt-tolerant microbial communities. By secreting organic acids and extracellular polysaccharides, they can lower soil pH, enhance plant resistance, and optimize soil structure. The production of saline-alkali land improvement microbial agents requires the use of nutrient solutions. For example, Chinese invention patent application number "CN202111253155.2" provides a microbial agent cultivation device. In this device, a mounting plate is slidably connected to the inner wall of the nutrient solution tank. Several cultivation groups are fixedly mounted on the mounting plate, each group comprising multiple incubators. The nutrient solution accumulates at the bottom of the nutrient solution tank. When nutrient solution needs to be added to an incubator, a linear drive mechanism moves the mounting plate downwards, and the stirring blades thoroughly agitate the nutrient solution, which automatically enters the storage tank and mixes fully with the microbial agent.

[0003] The drawback of this device is that the mounting plate completely covers the top opening of the nutrient solution tank. This prevents the addition of nutrient solution to the tank without removing the mounting plate and the incubator. Since the preparation and use of the saline-alkali land improvement agent requires multiple additions of nutrient solution, after a period of use, the nutrient solution level at the bottom of the tank will drop, preventing the nutrient solution from properly entering the storage tank and mixing fully with the agent. Summary of the Invention

[0004] The purpose of this invention is to provide a cultivation device and method for a microbial agent for improving saline-alkali land. The external nutrient solution can be directly introduced into the nutrient solution tank through the replenishment rod. The nutrient solution replenishment can be completed without removing the tank cover or the microbial agent cultivation component. The nutrient solution can always enter the microbial agent cultivation component normally and mix thoroughly with the microbial agent.

[0005] To achieve the above objectives, the present invention provides the following technical solution: In the first technical solution, a cultivation device for a saline-alkali land improvement microbial agent includes: a nutrient solution tank, the top of which is provided with a lid; a replenishment component, the replenishment component including a replenishment rod, the first end of which is located on the outside of the nutrient solution tank, the second end of which passes through the lid and extends to the bottom of the nutrient solution tank, the replenishment rod having a first replenishment chamber and a second replenishment chamber inside, a first electrically controlled valve being provided between the first replenishment chamber and the second replenishment chamber, and the second replenishment chamber communicating with the interior of the nutrient solution tank; and a microbial agent cultivation component, located inside the nutrient solution tank and connected to the output end of a positioning component.

[0006] In the first technical solution, preferably, the second end of the replenishing rod is provided with a plurality of stirring paddles, the interior of the stirring paddles is a cavity, the second replenishing cavity is connected to the interior of the stirring paddles, the side wall of the stirring paddles is provided with a plurality of liquid outlet holes, and the first end of the replenishing rod is connected to the output end of the rotary drive assembly.

[0007] In the first technical solution, preferably, the rotary drive assembly includes a pusher threaded rod, a dispensing chamber is provided at the center of the replenishing rod, the top of the dispensing chamber penetrates the top surface of the replenishing rod, the bottom of the pusher threaded rod is located in the dispensing chamber and connected to the pusher block, the top sidewall of the dispensing chamber is provided with an internal thread that mates with the pusher threaded rod, the bottom of the first replenishing chamber communicates with the bottom of the dispensing chamber, and the bottom of the dispensing chamber corresponds to the position of the first electrically controlled valve.

[0008] In the first technical solution, preferably, it further includes an infusion assembly, which includes an infusion cylinder sleeved on the outside of the push-pull threaded rod with a gap between them. The bottom of the infusion cylinder is rotatably connected to the top of the replenishment rod. The infusion cylinder has an infusion chamber inside, with an infusion tube at the top and a drain tube at the bottom. A second electrically controlled valve is provided in the middle of the infusion chamber. The top of the replenishment rod has an annular storage chamber, with the top of the first replenishment chamber communicating with the annular storage chamber. The bottom of the drain tube extends into the annular storage chamber.

[0009] In the first technical solution, preferably, the bacterial culture component includes a culture box, a culture chamber at the top of the culture box, an annular pipetting chamber at the bottom of the culture box, a pipetting channel and a pushing channel above the annular pipetting chamber, a first end of the pipetting channel and a first end of the pushing channel communicating with the annular pipetting chamber, a second end of the pipetting channel communicating with the culture chamber, a second end of the pushing channel penetrating through the top surface of the culture box, an inlet hole on the outer wall of the culture box communicating with the bottom of the pushing channel, and a pushing component in the middle of the pushing channel.

[0010] In the first technical solution, preferably, the top of the pipetting channel has an inverted V-shaped structure, and the width of the first end of the pipetting channel is greater than the width of the second end.

[0011] In the first technical solution, preferably, the liquid pushing assembly includes an air pump, the air outlet of the air pump is connected to the interior of the elastic air bladder, and a baffle is provided in the annular liquid transfer chamber and between the liquid transfer channel and the liquid pushing channel.

[0012] In the first technical solution, preferably, the baffle is fixedly disposed on the inner top surface of the annular pipetting cavity, the baffle is an arc-shaped plate, and the concave surface is located on the side close to the pipetting channel.

[0013] In the first technical solution, preferably, the culture chamber is equipped with a stirrer, and the inner wall of the nutrient solution tank is equipped with a stirring drive assembly, and the stirrer is connected to the output end of the stirring drive assembly.

[0014] In the second technical solution, a method for using a cultivation device for saline-alkali land improvement microbial agents, using the cultivation device for saline-alkali land improvement microbial agents as described in the first technical solution, includes the following steps: Step 1: Open the box cover and add microbial agents into the microbial agent cultivation component; Step 2: Open the first and second electrically controlled valves and add nutrient solution into the annular storage chamber through the infusion pipe; Step 3: Drive the pusher screw upward, causing the pusher screw to drive the replenishment rod and several of the stirring paddles to rotate in the opposite direction. After passing through the first replenishment chamber, part of the nutrient solution in the annular storage chamber enters the distribution chamber for storage, and the other part enters the second replenishment chamber and the interior of the stirring paddles through the distribution chamber, and then enters through the outlet hole. Step 4: Close the tank cover and the second electrically controlled valve, drive the pusher screw rod to move up and down reciprocally, so that the stirring paddle continuously stirs the nutrient solution in the nutrient solution tank, and drive the bacterial culture component to move downward through the adjustment component, so that the nutrient solution in the nutrient solution tank is added to the bacterial culture component. Then the adjustment component drives the bacterial culture component to move upward and reset. Step 5: When it is necessary to replenish the nutrient solution in the nutrient solution tank, open the second electrically controlled valve, and add nutrient solution to the annular storage chamber through the infusion tube. The nutrient solution in the annular storage chamber passes through the first replenishment chamber, the distribution chamber, the second replenishment chamber and the interior of the stirring paddle in sequence, and finally enters the nutrient solution tank through the outlet hole.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] (i) When using this invention, the external nutrient solution can be directly introduced into the nutrient solution tank through the replenishment rod. The nutrient solution replenishment operation can be completed without removing the tank cover or the bacterial culture component. The nutrient solution can always enter the bacterial culture component normally and mix fully with the bacterial agent.

[0017] (ii) When the present invention is in use, when the pusher screw moves upward, the nutrient solution at the bottom of the nutrient solution tank can enter the distribution chamber through the outlet hole. When the pusher screw moves downward, the pusher screw drives the pusher block to move downward. The pusher block slides along the inner wall of the distribution chamber and pushes the nutrient solution in the distribution chamber back into the nutrient solution tank through the outlet hole, thereby enhancing the flow effect and stirring effect of the nutrient solution.

[0018] (III) In use, when the positioning component moves the bacterial culture component downwards, the nutrient solution in the nutrient solution tank enters the annular pipetting chamber through the inlet hole. Subsequently, the pushing component in the pushing channel operates, pushing the nutrient solution in the annular pipetting chamber into the pipetting channel. The nutrient solution in the pipetting channel is then injected into the culture chamber, coming into contact with the bacterial agent. Finally, the stirrer operates to ensure thorough mixing of the nutrient solution and the bacterial agent. Attached Figure Description

[0019] Figure 1 This is an isometric view of the present invention;

[0020] Figure 2 A front sectional view of the present invention when the microbial culture component is located at the top inside the nutrient solution tank;

[0021] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0022] Figure 4 for Figure 2 Enlarged view of point B in the middle;

[0023] Figure 5 A front sectional view of the present invention when the microbial culture component is located at the bottom of the nutrient solution tank;

[0024] Figure 6 for Figure 5 Enlarged view of point C in the middle;

[0025] Figure 7 This is an isometric sectional view of the nutrient solution tank in this invention;

[0026] Figure 8 This is an isometric sectional view of the fluid replenishment component in this invention;

[0027] Figure 9 This is an isometric sectional view of the bacterial culture component in this invention;

[0028] Figure 10 This is an isometric view of the positioning component in this invention;

[0029] Figure 11 This is an isometric view of the rotary drive assembly in this invention;

[0030] Figure 12 This is an isometric view of the infusion assembly in this invention.

[0031] The reference numerals in the figures include:

[0032] 1-Nutrient solution tank, 11-Tank lid, 2-Replenishment component, 21-Replenishment rod, 211-First replenishment chamber, 212-Second replenishment chamber, 213-Dispensing chamber, 214-Internal thread, 215-Annular storage chamber, 22-First electrically controlled valve, 23-Agitator, 231-Dispensing port, 3-Inoculum culture component, 31-Cultivation box, 311-Cultivation chamber, 312-Annular pipetting chamber, 313-Pipeting channel, 314-Pushing channel, 315-Inlet, 316-Baffle, 32-Pushing component, 321-Air pump, 322-Elastic airbag 33-Agitator, 331-Agitator shaft, 3311-Threaded hole, 332-Agitator blade, 4-Adjustment assembly, 41-Bearing ring, 42-First adjustment frame, 43-First electric telescopic rod, 5-Rotary drive assembly, 51-Pushing threaded rod, 52-Pushing block, 53-Second adjustment frame, 54-Second electric telescopic rod, 6-Infusion assembly, 61-Infusion cylinder, 611-Infusion chamber, 62-Infusion tube, 63-Drain tube, 64-Second electrically controlled valve, 65-Extension plate, 7-Agitator drive assembly, 71-Positioning plate, 72-Adjustment threaded rod. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] Example 1

[0035] Please see Figure 1-12This invention provides a technical solution: a cultivation device for microbial agents used to improve saline-alkali land, comprising a nutrient solution tank 1, a replenishment component 2, a microbial agent cultivation component 3, and a positioning component 4. The nutrient solution tank 1 has a lid 11 on its top, which is detachably connected to the nutrient solution tank 1 and is suitable for both anaerobic and aerobic cultivation. The replenishment component 2 includes a replenishment rod 21 and a first electrically controlled valve 22. The replenishment rod 21 has a first replenishment chamber 211 and a second replenishment chamber 212 inside. In use, the lid 11 is opened to place the microbial agent into the microbial agent cultivation component 3, nutrient solution is added to the bottom of the nutrient solution tank 1, and then the lid 11 is closed. In this embodiment, the positioning component 4 includes a support ring 41, a first positioning frame 42, and a first electrically telescopic rod 43. Several microbial agent cultivation components 3 are arranged in a ring and are all fixedly connected to the support ring 41. The support ring 41 is connected to the bottom of the first adjusting frame 42, and the top of the first adjusting frame 42 passes through the box cover 11 and is connected to the output end of the first electric telescopic rod 43. The first adjusting frame 42 is slidably connected to the box cover 11. If the output end of the first electric telescopic rod 43 shortens, it can drive several bacterial culture components 3 to move downwards; if the output end of the first electric telescopic rod 43 extends, it can drive several bacterial culture components 3 to move upwards. When it is necessary to add nutrient solution to the bacterial agent, the adjusting component 4 works, driving the bacterial culture components 3 to move downwards, so that the bottom of the bacterial culture components 3 moves below the liquid surface of the nutrient solution at the bottom of the nutrient solution tank 1. The nutrient solution can enter the interior of the bacterial culture components 3 and mix thoroughly with the bacterial agent. Then, the adjusting component 4 drives the bacterial culture components 3 to move upwards and reset.

[0036] As the above operation is repeated multiple times, the nutrient solution level at the bottom of the nutrient solution tank 1 gradually decreases. At this point, the first electrically controlled valve 22 is opened, and external nutrient solution is then delivered to the first replenishment chamber 211. The external nutrient solution can move from the top to the bottom of the replenishment rod 21, pass through the first electrically controlled valve 22, and enter the second replenishment chamber 212, ultimately entering the nutrient solution tank 1, causing the nutrient solution level at the bottom of the nutrient solution tank 1 to gradually rise. Therefore, external nutrient solution can directly enter the interior of the nutrient solution tank 1 through the replenishment rod 21, completing the nutrient solution replenishment operation without removing the tank cover 11 or the bacterial culture component 3. The nutrient solution can always enter the bacterial culture component 3 normally and mix thoroughly with the bacterial agent.

[0037] Example 2

[0038] Based on Example 1, please refer to Figure 1-2 and Figure 7-8The bottom of the replenishing rod 21 is equipped with several stirring paddles 23, and the side walls of the stirring paddles 23 are equipped with several outlet holes 231. The nutrient solution in the second replenishing chamber 212 first enters the cavity inside the stirring paddle 23, and then enters the interior of the nutrient solution tank 1 through the outlet holes 231. The top of the replenishing rod 21 is connected to the output end of the rotary drive assembly 5. When the nutrient solution is left for a period of time, the elements in the nutrient solution will precipitate. At this time, the rotary drive assembly 5 works, driving the replenishing rod 21 and the stirring paddles 23 to rotate continuously, which plays the role of stirring the nutrient solution. When the elements in the nutrient solution are evenly distributed, the positioning assembly 4 then drives the bacterial culture assembly 3 to move downward.

[0039] Please see Figure 1-2 , Figure 4 , Figure 7-8 and Figure 11 The rotary drive assembly 5 includes a pusher threaded rod 51, a pusher block 52, a second adjusting frame 53, and a second electric telescopic rod 54. A dispensing chamber 213 is located at the center of the replenishing rod 21, and the top sidewall of the dispensing chamber 213 has an internal thread 214 that mates with the pusher threaded rod 51. The output end of the second electric telescopic rod 54 extends, causing the second adjusting frame 53, the pusher threaded rod 51, and the pusher block 52 to move upwards together. At this time, the pusher threaded rod 51 drives the replenishing rod 21 to rotate in the opposite direction. A portion of the nutrient solution in the first replenishing chamber 211 enters the dispensing chamber 213 for storage, while another portion enters the second replenishing chamber 212 and the interior of the stirring paddle 23 through the dispensing chamber 213, and then enters the nutrient solution tank 1 through the outlet hole 231. When elements in the nutrient solution precipitate, the output end of the second electric telescopic rod 54 continuously switches between extended and shortened states, driving the pusher threaded rod 51 to reciprocate up and down, causing the stirring paddle 23 to repeatedly switch between forward and reverse rotation, continuously stirring the nutrient solution in the nutrient solution tank 1. When the pusher threaded rod 51 moves upward, the nutrient solution at the bottom of the nutrient solution tank 1 can enter the distribution chamber 213 through the outlet hole 231. When the pusher threaded rod 51 moves downward, it drives the pusher block 52 to move downward. The pusher block 52 slides along the inner wall of the distribution chamber 213 and pushes the nutrient solution in the distribution chamber 213 back into the nutrient solution tank 1 through the outlet hole 231, enhancing the flow and stirring effects of the nutrient solution. In this embodiment, the movement of the pusher threaded rod 51 is electrically controlled; in other embodiments, the pusher threaded rod 51 can also be manually controlled.

[0040] Please see Figure 1-2 , Figure 4 , Figure 7-8 and Figure 11-12The top of the fluid replenishment assembly 2 is equipped with an infusion assembly 6. Since the position of the first fluid replenishment chamber 211 changes when the fluid replenishment rod 21 rotates, the infusion assembly 6 ensures that, regardless of the position of the first fluid replenishment chamber 211, external nutrient solution can enter the interior of the fluid replenishment rod 21 from the same position during nutrient replenishment. The infusion assembly 6 includes an infusion cylinder 61, an infusion tube 62, a drain tube 63, a second electrically controlled valve 64, and an expansion plate 65. The infusion cylinder 61 has an infusion chamber 611 inside, and the top of the fluid replenishment rod 21 has an annular storage chamber 215. The bottom of the drain tube 63 extends into the annular storage chamber 215. The bottom of the infusion cylinder 61 is rotatably connected to the top of the fluid replenishment rod 21, so the infusion cylinder 61 remains stationary when the fluid replenishment rod 21 rotates. When nutrient solution needs to be replenished, the external nutrient solution first enters the infusion chamber 611 through the infusion tube 62. After the second electrically controlled valve 64 is opened, the nutrient solution in the infusion chamber 611 first passes through the second electrically controlled valve 64, and then enters the annular storage chamber 215 through the drain tube 63. At this time, regardless of the position of the first replenishment chamber 211, the nutrient solution in the annular storage chamber 215 can enter the first replenishment chamber 211, then enter the second replenishment chamber 212 and the dispensing chamber 213, and finally enter the nutrient solution tank 1. In this embodiment, the infusion cylinder 61 is sleeved on the outside of the push threaded rod 51, and there is a gap between them. Therefore, when the push threaded rod 51 moves, it will not interfere with the infusion cylinder 61. When the push threaded rod 51 moves, the second electrically controlled valve 64 is in the closed state, so the nutrient solution in the nutrient solution tank 1 will not enter the infusion tube 62, and the infusion tube 62 will not be contaminated. The expansion plate 65 is fixedly mounted on the outer wall of the infusion cylinder 61 and is fixedly connected to the external limiting mechanism. A snap-fit ​​block and a snap-fit ​​groove are installed between the expansion plate 65 and the cover 11 (the external limiting mechanism, the snap-fit ​​block and the snap-fit ​​groove are not shown in the figure). When the cover 11 is opened and moved upward, the cover 11 can be fixed together with the expansion plate 65, which facilitates the placement of microbial agents into the bacterial agent culture component 3.

[0041] Example 3

[0042] Based on Example 1, please refer to Figure 1-7 and Figure 9The bacterial culture component 3 includes a culture box 31, a dispensing component 32, and a stirrer 33. The culture box 31 has a culture chamber 311 at its top and an annular dispensing chamber 312 at its bottom. Above the annular dispensing chamber 312 are a dispensing channel 313 and a dispensing channel 314. An inlet hole 315 is located on the outer wall of the culture box 31. When the adjusting component 4 moves the bacterial culture component 3 downwards, the nutrient solution level at the bottom of the nutrient solution tank 1 will submerge the inlet hole 315, allowing the nutrient solution in the nutrient solution tank 1 to enter the annular dispensing chamber 312 through the inlet hole 315. Subsequently, the dispensing component 32 in the dispensing channel 314 operates, pushing the nutrient solution in the annular dispensing chamber 312 into the dispensing channel 313. The nutrient solution in the dispensing channel 313 then flows into the culture chamber 311, contacting the bacterial agent. Finally, the stirrer 33 operates, ensuring thorough mixing of the nutrient solution and the bacterial agent.

[0043] Please see Figure 3 , Figure 6 and Figure 9 The top of the pipetting channel 313 has an inverted V-shaped structure. When the nutrient solution moves to the top of the pipetting channel 313, it will be ejected downwards, allowing the nutrient solution to quickly reach the bottom of the bacterial culture chamber 311. The width of the first end of the pipetting channel 313 is greater than the width of the second end. Therefore, the speed of the nutrient solution in the pipetting channel 313 gradually increases as it moves upwards, giving the nutrient solution ejected from the pipetting channel 313 sufficient kinetic energy.

[0044] Please see Figure 3 , Figure 6 and Figure 9 The liquid dispensing assembly 32 includes an air pump 321 and an elastic airbag 322, with a baffle 316 inside the annular dispensing chamber 312. When the liquid dispensing assembly 32 is working, the air pump 321 draws air from the top of the nutrient solution tank 1 and the dispensing channel 314 into the elastic airbag 322. After the elastic airbag 322 inflates, it first blocks the inlet hole 315, and then pushes the nutrient solution in the annular dispensing chamber 312 into the dispensing channel 313. The baffle 316 is an arc-shaped plate, and its concave surface is located on the side near the dispensing channel 313. Therefore, the elastic airbag 322 can slide along the convex surface of the baffle 316 without the baffle 316 scratching the elastic airbag 322. The baffle 316 can limit the elastic airbag 322, preventing it from blocking the bottom opening of the dispensing channel 313. After the nutrient solution and bacterial agent are fully mixed, the air pump 321 draws the air out of the elastic air bag 322 and discharges it into the nutrient solution tank 1. The elastic air bag 322 contracts, and the liquid inlet 315 is no longer blocked.

[0045] Please see Figure 1-7 and Figure 9-10The stirrer 33 includes a stirring shaft 331 and stirring blades 332. The bottom of the stirring shaft 331 is rotatably connected to the bottom surface of the culture chamber 311, and the top of the stirring shaft 331 is provided with a threaded hole 3311. A stirring drive assembly 7 is provided on the inner wall of the nutrient solution tank 1. The stirring drive assembly 7 includes a positioning plate 71 and an adjusting threaded rod 72. The positioning plate 71 is fixedly connected to the inner wall of the nutrient solution tank 1, and one end of the adjusting threaded rod 72 is fixedly connected to the positioning plate 71, while the other end is screwed into the threaded hole 3311. When the adjusting assembly 4 moves the bacterial culture assembly 3 downwards, the stirring shaft 331 gradually moves away from the adjusting threaded rod 72. At this time, the adjusting threaded rod 72 drives the stirring shaft 331 and the stirring blades 332 to rotate in the opposite direction, and the stirring blades 332 stir the bacterial culture in the culture chamber 311. When the adjusting assembly 4 moves the bacterial culture assembly 3 upwards, the stirring shaft 331 gradually approaches the adjusting threaded rod 72. At this time, the adjusting screw rod 72 drives the stirring shaft 331 and the stirring blade 332 to rotate in the forward direction together. The stirring blade 332 stirs the nutrient solution and bacterial agent in the culture chamber 311, so that the nutrient solution and bacterial agent are fully mixed.

[0046] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0047] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A cultivation device for a saline-alkali land improvement microbial agent, characterized in that, include: A nutrient solution tank, the top of which is equipped with a lid; A fluid replenishment assembly includes a fluid replenishment rod. The first end of the fluid replenishment rod is located on the outside of the nutrient solution tank, and the second end of the fluid replenishment rod passes through the tank cover and extends to the bottom inside the nutrient solution tank. The fluid replenishment rod has a first fluid replenishment chamber and a second fluid replenishment chamber inside. A first electrically controlled valve is provided between the first fluid replenishment chamber and the second fluid replenishment chamber. The second fluid replenishment chamber is in communication with the inside of the nutrient solution tank. The microbial culture component is located inside the nutrient solution tank and is connected to the output end of the adjustment component; The second end of the replenishing rod is provided with several stirring paddles, the interior of the stirring paddles is a cavity, the second replenishing cavity is connected to the interior of the stirring paddles, the side wall of the stirring paddles is provided with several liquid outlet holes, and the first end of the replenishing rod is connected to the output end of the rotary drive assembly. The rotary drive assembly includes a pusher threaded rod, a dispensing chamber is provided at the center of the replenishing rod, the top of the dispensing chamber penetrates the top surface of the replenishing rod, the bottom of the pusher threaded rod is located in the dispensing chamber and connected to the pusher block, the top side wall of the dispensing chamber is provided with an internal thread that mates with the pusher threaded rod, the bottom of the first replenishing chamber communicates with the bottom of the dispensing chamber, and the bottom of the dispensing chamber corresponds to the position of the first electrically controlled valve; It also includes an infusion assembly, which includes an infusion cylinder sleeved on the outside of the push-pull threaded rod with a gap between them. The bottom of the infusion cylinder is rotatably connected to the top of the replenishment rod. The infusion cylinder has an infusion chamber inside, with an infusion tube at the top and a drain tube at the bottom. A second electrically controlled valve is located in the middle of the infusion chamber. The top of the replenishment rod has an annular storage chamber. The top of the first replenishment chamber communicates with the annular storage chamber, and the bottom of the drain tube extends into the annular storage chamber.

2. The cultivation device for the saline-alkali land improvement microbial agent according to claim 1, characterized in that, The microbial culture assembly includes a culture box with a culture chamber at the top and an annular pipetting chamber at the bottom. Above the annular pipetting chamber are a pipetting channel and a push channel. The first end of the pipetting channel and the first end of the push channel are both connected to the annular pipetting chamber. The second end of the pipetting channel is connected to the culture chamber. The second end of the push channel penetrates the top surface of the culture box. An inlet hole is provided on the outer wall of the culture box, which is connected to the bottom of the push channel. A push assembly is provided in the middle of the push channel.

3. The cultivation device for the saline-alkali land improvement microbial agent according to claim 2, characterized in that, The top of the pipetting channel has an inverted V-shaped structure, and the width of the first end of the pipetting channel is greater than the width of the second end.

4. The cultivation device for the saline-alkali land improvement microbial agent according to claim 3, characterized in that, The liquid pushing assembly includes an air pump, the air outlet of which is connected to the interior of the elastic air bladder, and a baffle is provided inside the annular liquid transfer chamber and between the liquid transfer channel and the liquid pushing channel.

5. The cultivation device for the saline-alkali land improvement microbial agent according to claim 4, characterized in that, The baffle is fixedly disposed on the inner top surface of the annular pipetting cavity. The baffle is an arc-shaped plate, and the concave surface is located on the side close to the pipetting channel.

6. The cultivation device for the saline-alkali land improvement microbial agent according to claim 3, characterized in that, The culture chamber is equipped with a stirrer, and the inner wall of the nutrient solution tank is equipped with a stirring drive assembly. The stirrer is connected to the output end of the stirring drive assembly.

7. A method of using a cultivation device for saline-alkali land improving microbial agents, comprising using the cultivation device for saline-alkali land improving microbial agents as described in claim 1, characterized in that, Includes the following steps: Step 1: Open the box lid and add bacterial agent into the bacterial agent culture component; Step 2: Open the first and second electrically controlled valves, and add nutrient solution into the annular storage chamber through the infusion tube; Step 3: Drive the pusher screw upward, so that the pusher screw drives the replenishment rod and several of the stirring paddles to rotate in the opposite direction. After the nutrient solution in the annular storage chamber passes through the first replenishment chamber, part of it enters the distribution chamber for storage, and the other part enters the second replenishment chamber and the interior of the stirring paddle through the distribution chamber, and then enters the nutrient solution tank through the outlet hole. Step 4: Close the tank cover and the second electrically controlled valve, drive the pusher screw rod to move up and down reciprocally, so that the stirring paddle continuously stirs the nutrient solution in the nutrient solution tank, and drive the bacterial culture component to move downward through the adjustment component, so that the nutrient solution in the nutrient solution tank is added to the bacterial culture component. Then the adjustment component drives the bacterial culture component to move upward to reset. Step 5: When it is necessary to replenish the nutrient solution in the nutrient solution tank, open the second electrically controlled valve and add nutrient solution to the annular storage chamber through the infusion tube. The nutrient solution in the annular storage chamber passes through the first replenishment chamber, the distribution chamber, the second replenishment chamber and the interior of the stirring paddle in sequence, and finally enters the nutrient solution tank through the outlet hole.

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