Cellular assay plate

Abstract

The application provides a cell detection plate and belongs to the technical field of cell detection. The cell detection plate comprises a detection plate body, and a tilting mechanism for driving the detection plate to tilt is arranged below the detection plate body. The supernatant extracted from a cell culture tank can be conveyed to the inside of the third tower head through a pipeline, the second tower head is communicated with a diluent through a pipeline, the diluent flows into the second flow channel, the supernatant flows into the third flow channel, and then flows into the inside of the first tower head from the first flow channel after passing through the detection area. Through the arrangement of multiple groups of converging points, the supernatant and the diluent can be fully mixed and uniformly mixed, so that the cells in the inside of the detection plate body can be detected, thereby solving the problem that the cells may be contaminated when the cells are exposed to air during the transfer process during cell detection.

Description

Technical Field

[0001] This application relates to the field of cell detection, and more specifically, to cell detection plates. Background Technology

[0002] Cell detection chips are miniaturized devices used for rapid and efficient detection and analysis of cells, and are widely used in biomedical research, clinical diagnostics, and drug development. Cell detection chips have broad application prospects in the biomedical field, and with technological advancements, they will play an even greater role in disease diagnosis and drug screening in the future.

[0003] In existing technologies, cell sampling and detection are usually performed by injecting the sample into a cell detection plate using a pipette, and then detecting it using a cell counter or by dropping it onto a glass slide for observation under a microscope.

[0004] However, existing cell detection devices still have the following shortcomings during use: During the transfer process of existing cell detection devices, the cells may be contaminated due to exposure to air. Utility Model Content

[0005] To address the above deficiencies, this application provides a cell detection plate, which aims to improve the risk of cell contamination during the transfer process when cells are exposed to air.

[0006] This application provides a cell detection plate, including a detection plate body. A tilting mechanism for tilting the detection plate is provided below the detection plate body. A detection platform is provided below the detection plate body. A fixing mechanism for preventing the detection plate body from moving is provided above the detection platform.

[0007] The detection plate body includes an upper plate, a lower plate is fixedly connected to the bottom of the upper plate, a first pagoda head, a second pagoda head and a third pagoda head are connected to the top of the upper plate, and a first flow channel, a second flow channel, a third flow channel and a detection area are opened at the bottom of the upper plate.

[0008] In one specific implementation, the first pagoda head is connected to one end of the first flow channel, the second pagoda head is connected to one end of the second flow channel, the third pagoda head is connected to one end of the third flow channel, the other ends of the second and third flow channels are connected to one end of the detection area, and the other end of the detection area is connected to the other end of the first flow channel.

[0009] In the above implementation process, by setting the second pagoda head, diluent can be introduced into the second pagoda head, and supernatant containing the cells to be tested can be introduced into the third pagoda head. The diluent flows into the second flow channel, the supernatant flows into the third flow channel, and after passing through the detection area, it flows into the interior of the first pagoda head from the first flow channel.

[0010] In one specific implementation, the second and third flow channels are provided with multiple sets of confluence points.

[0011] In the above process, by setting up multiple sets of confluence points, the supernatant and diluent can be fully mixed, resulting in a more uniform mixture.

[0012] In one specific implementation, the tilting mechanism includes four sets of vertical rods. The vertical rods are hinged to the bottom of the lower plate. The other end of the vertical rod is connected to a first circular plate. The bottom of the first circular plate is connected to a telescopic damping rod. The other end of the telescopic damping rod is connected to a second circular plate. A first spring is sleeved on the outer surface of the telescopic damping rod.

[0013] In the above implementation process, by setting the first spring, the detection plate body can be tilted under force, which will compress the first spring and the telescopic damping rod. After no liquid enters the second and third flow channels, the detection plate body can be tilted to allow the solution inside the detection area, the first flow channel, the second flow channel and the third flow channel to enter the interior of the first pagoda head through the first flow channel.

[0014] In one specific implementation, four sets of pressing plates are connected to the outer surface of the upper plate.

[0015] In the above implementation process, by setting up the pressing plate, the pressing plate can be manually pressed, causing one side of the detection plate body to tilt.

[0016] In one specific implementation, the fixing mechanism includes four sets of fixing members. The top of each fixing member has a slot, and a card is movably inserted into the slot. The card is connected to the bottom of the second circular plate.

[0017] In the above implementation process, by setting the card slot, the card can be fixed inside the card slot, so that when people apply force to press the pressing plate, the detection plate will not move back and forth or left and right.

[0018] In one specific implementation, a housing is connected to one side of the fixing member, a movable plate is slidably connected to the inner wall of the housing, a pull rod is connected to one side of the movable plate, two sets of pull rods are connected to a set of pull plates, and a second spring is sleeved on the outer surface of the pull rod.

[0019] In the above implementation process, by setting up the pull plate, the pull rod can be moved by pulling the pull plate, which in turn moves the moving plate and compresses the second spring. After the pull plate is no longer under tension, the second spring releases its elastic potential energy and drives the moving plate to reset.

[0020] In one specific implementation, a positioning hole is provided through one side of the card.

[0021] In the above implementation process, by setting the positioning hole, the positioning element can be inserted into the positioning hole to limit the card inside the card slot and prevent the card from falling out of the card slot.

[0022] In one specific implementation, a positioning member is connected to the other side of the movable plate, one end of which passes through the fixing member and is movably inserted into the positioning hole.

[0023] In the above implementation process, by setting the positioning component, the positioning component can be moved when the moving plate moves, so that the positioning component can move away from the positioning hole, thereby releasing the positioning component from the limiting position of the card, and the card can be released from the card slot.

[0024] In one specific implementation, both the upper and lower plates are made of transparent glass.

[0025] In the above process, both the upper and lower plates are made of transparent glass, which makes it easy for the testing personnel to observe the cells inside the testing plate.

[0026] Compared with the prior art, the beneficial effects of this application are as follows:

[0027] By designing the detection plate itself, the supernatant extracted from the cell culture tank can be transported through pipes to the interior of the third pagoda head. The second pagoda head is connected to a diluent through pipes, which flows into the second flow channel, while the supernatant flows into the third flow channel. After passing through the detection zone, it flows into the interior of the first pagoda head from the first flow channel. The multiple confluence points ensure that the supernatant and diluent are fully mixed, resulting in a more uniform mixture. This allows cells to be detected inside the detection plate itself, thus solving the problem of potential cell contamination risks during cell detection when cells are exposed to air during transit. Attached Figure Description

[0028] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0029] Figure 1 This is a schematic diagram of the cell detection plate provided in the embodiments of this application;

[0030] Figure 2 A schematic diagram of the first pagoda head structure provided for the embodiments of this application;

[0031] Figure 3 A schematic diagram of the pressing plate structure provided for an embodiment of this application;

[0032] Figure 4 A schematic diagram of the lower plate structure provided for an embodiment of this application;

[0033] Figure 5 for Figure 4 Enlarged view of point A in the middle;

[0034] Figure 6 A schematic diagram of the positioning hole structure provided for an embodiment of this application;

[0035] Figure 7 A schematic diagram of the fastener structure provided for an embodiment of this application;

[0036] Figure 8 A schematic diagram of the second spring structure provided for an embodiment of this application.

[0037] In the diagram: 1. Detection plate body; 101. Upper plate; 102. Lower plate; 103. First pagoda head; 104. Second pagoda head; 105. Detection area; 106. First flow channel; 107. Second flow channel; 108. Third pagoda head; 109. Third flow channel; 2. Tilting mechanism; 201. Pressing plate; 202. Vertical rod; 203. First circular plate; 204. Clamping device; 205. Second circular plate; 206. First spring; 207. Telescopic damping rod; 3. Fixing mechanism; 301. Positioning hole; 302. Fixing component; 303. Housing; 304. Slot; 305. Moving plate; 306. Positioning component; 307. Second spring; 308. Pull rod; 309. Pull plate; 4. Detection table. Detailed Implementation

[0038] The present invention will be described more clearly and completely below with reference to the accompanying drawings, using a preferred embodiment.

[0039] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0040] Please see Figure 1 This application provides a cell detection plate, including a detection plate body 1.

[0041] Please see Figure 1 and Figure 2 The detection plate body 1 is provided with a tilting mechanism 2 for tilting the detection plate below it, and a detection platform 4 is provided below the detection plate body 1. A fixing mechanism 3 is provided above the detection platform 4 to prevent the detection plate body 1 from moving.

[0042] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 The detection plate body 1 includes an upper plate 101, a lower plate 102 is fixedly connected to the bottom of the upper plate 101, a first pagoda head 103, a second pagoda head 104 and a third pagoda head 108 are connected to the top of the upper plate 101, and a first flow channel 106, a second flow channel 107, a third flow channel 109 and a detection area 105 are opened at the bottom of the upper plate 101.

[0043] In the specific configuration, the first pagoda head 103 is connected to one end of the first flow channel 106, the second pagoda head 104 is connected to one end of the second flow channel 107, and the third pagoda head 108 is connected to one end of the third flow channel 109. The other ends of the second flow channel 107 and the third flow channel 109 are connected to one end of the detection area 105, and the other end of the detection area 105 is connected to the other end of the first flow channel 106. Through the configuration of the second pagoda head 104, diluent can be introduced into the second pagoda head 104, and supernatant containing the cells to be tested can be introduced into the third pagoda head 108. The diluent flows into the second flow channel 107, and the supernatant flows into the third flow channel 109. After passing through the detection area 105, it flows into the interior of the first pagoda head 103 from the first flow channel 106.

[0044] In the specific configuration, the second flow channel 107 and the third flow channel 109 are provided with multiple sets of confluence points. The multiple sets of confluence points enable the supernatant and diluent to be fully mixed, resulting in a more uniform mixing.

[0045] In a specific configuration, the tilting mechanism 2 includes four sets of vertical rods 202. The vertical rods 202 are hinged to the bottom of the lower plate 102. The other end of the vertical rods 202 is connected to a first circular plate 203. The bottom of the first circular plate 203 is connected to a telescopic damping rod 207. The other end of the telescopic damping rod 207 is connected to a second circular plate 205. A first spring 206 is sleeved on the outer surface of the telescopic damping rod 207. The first spring 206 can compress the first spring 206 and the telescopic damping rod 207 when the detection plate body 1 is tilted under force. After no liquid enters the second flow channel 107 and the third flow channel 109, the solution inside the detection area 105, the first flow channel 106, the second flow channel 107 and the third flow channel 109 can enter the interior of the first pagoda head 103 through the first flow channel 106 by tilting the detection plate body 1.

[0046] In a specific configuration, four sets of pressing plates 201 are connected to the outer surface of the upper plate 101. By setting the pressing plates 201, one side of the detection plate body 1 can be tilted by manually pressing the pressing plates 201.

[0047] In a specific configuration, the fixing mechanism 3 includes four sets of fixing members 302. The top of the fixing member 302 is provided with a slot 304, and a card 204 is movably inserted into the slot 304. The card 204 is connected to the bottom of the second circular plate 205. By setting the slot 304, the card 204 can be fixed inside the slot 304, so that when people apply force to press the pressing plate 201, the detection plate will not move back and forth or left and right.

[0048] In the specific configuration, a housing 303 is connected to one side of the fixing member 302, and a movable plate 305 is slidably connected to the inner wall of the housing 303. A pull rod 308 is connected to one side of the movable plate 305, and a set of pull plates 309 are connected to two sets of pull rods 308. A second spring 307 is sleeved on the outer surface of the pull rod 308. By setting the pull plate 309, the pull rod 308 can be moved by pulling the pull plate 309, which in turn moves the movable plate 305, compressing the second spring 307. After the pull plate 309 is no longer under tension, the second spring 307 releases its elastic potential energy, causing the movable plate 305 to return to its original position.

[0049] In a specific configuration, a positioning hole 301 is provided through one side of the card 204. By inserting the positioning member 306 into the positioning hole 301, the card 204 can be limited to the inside of the card slot 304, thus preventing the card 204 from detaching from the card slot 304.

[0050] In a specific configuration, a positioning member 306 is connected to the other side of the movable plate 305. One end of the positioning member 306 passes through the fixing member 302 and is movably inserted into the positioning hole 301. By setting the positioning member 306, when the movable plate 305 moves, the positioning member 306 can be moved away from the positioning hole 301, thereby releasing the positioning member 306 from limiting the card 204, and the card 204 can then disengage from the card slot 304.

[0051] In the specific setup, both the upper plate 101 and the lower plate 102 are made of transparent glass. The fact that both the upper plate 101 and the lower plate 102 are made of transparent glass makes it easy for the testing personnel to observe the cells inside the testing plate body 1.

[0052] The working principle of this cell detection plate is as follows: When using the cell detection plate, during the extraction of the supernatant, the supernatant extracted from the cell culture tank is transported through a pipe to the interior of the third pagoda head 108. The second pagoda head 104 is connected to a diluent through a pipe. The diluent flows into the second flow channel 107, and the supernatant flows into the third flow channel 109. After passing through the detection zone 105, it flows into the interior of the first pagoda head 103 from the first flow channel 106. The setting of multiple confluence points allows the supernatant and diluent to be fully mixed, resulting in a more uniform mixture. This allows the cells to be detected inside the detection plate body 1. After no liquid enters the second flow channel 107 and the third flow channel 109, by manually pressing the pressing plate 201, one side of the detection plate body 1 is tilted, causing the detection zone 105 and the first pagoda head 103 to be detected. The solution inside the flow channel 106, the second flow channel 107, and the third flow channel 109 enters the interior of the first pagoda head 103 through the first flow channel 106. By inserting the positioning member 306 into the positioning hole 301, the locking member 204 is limited inside the slot 304, preventing the locking member 204 from disengaging from the slot 304. Thus, when people apply force to press the pressing plate 201, the detection plate will not move back, forth, left, or right. Alternatively, by pulling the pull plate 309, the pull rod 308 can be moved, which in turn moves the moving plate 305, compressing the second spring 307. This allows the positioning member 306 to move away from the positioning hole 301, thereby releasing the positioning member 306 from limiting the locking member 204. The locking member 204 can then disengage from the slot 304, disconnecting the detection plate body 1 from the detection platform 4.

[0053] The above are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A cell detection plate, characterized in that, It includes The detection plate body (1) is provided with a tilting mechanism (2) for tilting the detection plate below the detection plate body (1), and a detection platform (4) is provided below the detection plate body (1). A fixing mechanism (3) for preventing the detection plate body (1) from moving is provided above the detection platform (4). The detection plate body (1) includes an upper plate (101), a lower plate (102) is fixedly connected to the bottom of the upper plate (101), a first pagoda head (103), a second pagoda head (104) and a third pagoda head (108) are connected to the top of the upper plate (101), and a first flow channel (106), a second flow channel (107), a third flow channel (109) and a detection area (105) are opened at the bottom of the upper plate (101).

2. The cell detection plate according to claim 1, characterized in that, The first pagoda head (103) is connected to one end of the first flow channel (106), the second pagoda head (104) is connected to one end of the second flow channel (107), the third pagoda head (108) is connected to one end of the third flow channel (109), the other ends of the second flow channel (107) and the third flow channel (109) are connected to one end of the detection area (105), and the other end of the detection area (105) is connected to the other end of the first flow channel (106).

3. The cell detection plate according to claim 1, characterized in that, The second flow channel (107) and the third flow channel (109) are provided with multiple sets of confluence points.

4. The cell detection plate according to claim 1, characterized in that, The tilting mechanism (2) includes four sets of vertical rods (202). The vertical rods (202) are hinged to the bottom of the lower plate (102). The other end of the vertical rods (202) is connected to a first circular plate (203). The bottom of the first circular plate (203) is connected to a telescopic damping rod (207). The other end of the telescopic damping rod (207) is connected to a second circular plate (205). A first spring (206) is sleeved on the outer surface of the telescopic damping rod (207).

5. The cell detection plate according to claim 1, characterized in that, Four sets of pressing plates (201) are connected to the outer surface of the upper plate (101).

6. The cell detection plate according to claim 1, characterized in that, The fixing mechanism (3) includes a fixing member (302), and the number of fixing members (302) is four. A slot (304) is provided on the top of the fixing member (302). A card (204) is movably inserted into the slot (304). The card (204) is connected to the bottom of the second circular plate (205).

7. The cell detection plate according to claim 6, characterized in that, The fixing member (302) is connected to a housing (303) on one side. A movable plate (305) is slidably connected to the inner wall of the housing (303). A pull rod (308) is connected to one side of the movable plate (305). A pull plate (309) is connected to two sets of pull rods (308). A second spring (307) is sleeved on the outer surface of the pull rod (308).

8. The cell detection plate according to claim 6, characterized in that, A positioning hole (301) is provided through one side of the card (204).

9. The cell detection plate according to claim 7, characterized in that, The other side of the movable plate (305) is connected to a positioning member (306), one end of which passes through the fixing member (302) and is movably inserted into the positioning hole (301).

10. The cell detection plate according to claim 1, characterized in that, Both the upper plate (101) and the lower plate (102) are made of transparent glass.

Images