Preparation method of copper-based metal complex and application thereof in antioxidant activity

Abstract

The application discloses a preparation method of a copper-based metal complex and application of the copper-based metal complex in antioxidation activity, relates to the technical field of functional materials, and has the chemical formula [Cu(H3dhbdc)2(H2O)2], wherein H3dhbdc is deprotonated 2,3-dihydroxyterephthalic acid. The copper-based metal complex has specific crystal structure and spatial structure, high synthesis purity, and good stability, and can be used in antioxidation activity. Under the same condition, the scavenging ability of H4dhbdc and the copper-based metal complex on •OH and O2 •‑ is explored, the copper-based metal complex is equivalent to H4dhbdc in terms of the scavenging effect on •OH free radicals, and is better than H4dhbdc in terms of the scavenging effect on O2 •‑ free radicals, and it is shown that the complex Cu 2+ after complexation not only retains the antioxidation activity of H4dhbdc, but also enhances the enzyme activity of H4dhbdc.

Description

Technical Field

[0001] This invention relates to the field of functional materials technology, and more specifically, to a method for preparing copper-based metal complexes and their application in antioxidant activity. Background Technology

[0002] In enzyme-mediated cellular metabolism, oxygen undergoes a series of single-electron reductions leading to the generation of reactive oxygen species (ROS). Excessively high ROS levels disrupt redox balance, causing oxidative stress and severe damage to the structure and function of macromolecules within cells. Existing natural enzymes are limited in their application for antioxidant and anti-inflammatory purposes due to their high cost, low yield, and instability. Nanozymes, with their superior catalytic activity and stability compared to natural enzymes, can serve as alternatives. Metal-based antioxidant materials containing polyphenolic compounds exhibit strong antioxidant and anti-inflammatory effects due to their phenolic hydroxyl groups and possess high biocompatibility, making them a major focus of current biomedical research.

[0003] Metal-organic materials are coordination materials formed by the linkage of metal ions or metal clusters with organic ligands through various interactions, exhibiting high biocompatibility. Combining the characteristics of inorganic and organic materials, and utilizing the antioxidant and low-toxicity properties of polyphenols and the antibacterial properties of metal ions, metal-organic materials are gradually becoming prominent candidates in the fields of antioxidant and anti-inflammatory applications.

[0004] No effective solutions have yet been proposed to address the problems in the relevant technologies. Summary of the Invention

[0005] To address the problems in related technologies, this invention proposes a method for preparing copper-based metal complexes and their application in antioxidant activity, thereby overcoming the aforementioned technical problems existing in the prior art.

[0006] Therefore, the specific technical solution adopted by the present invention is as follows:

[0007] According to a first aspect of the present invention, a copper-based metal complex is provided, the chemical formula of which is:

[0008] [Cu(H3dhbdc)2(H2O)2], where H3dhbdc is deprotonated 2,3-dihydroxyterephthalic acid.

[0009] Furthermore, the copper-based metal complex has a green, rice-grain-like crystal form and a specific crystal structure and spatial structure.

[0010] Furthermore, the crystal structure parameters of the copper-based metal complex include: space group triclinic P-1; unit cell parameters: a = 6.8864(2) Å, b = 6.9466(2) Å, c = 9.6984(3) Å, α = 69.266°, β = 75.085°, γ = 76.367°; and unit cell volume of 413.88(2) Å. 3 The number of asymmetric units Z in the unit cell is 2.

[0011] According to a second aspect of the present invention, a method for preparing a copper-based metal complex is provided, the method comprising the following steps:

[0012] S1. Mix H2O and MeOH to prepare a mixed solvent;

[0013] S2. Cu(NO3)2·3H2O and H4dhbdc are added to the obtained mixed solvent and reacted under preset conditions to obtain green rice-grain-shaped crystals.

[0014] Furthermore, H2O and MeOH are mixed in a molar ratio of 1:1.

[0015] Furthermore, the molar ratio of Cu(NO3)2·3H2O to H4dhbdc is 1:1.

[0016] Furthermore, the preset conditions are:

[0017] The reaction was carried out at a constant temperature of 70℃ for 2 days.

[0018] According to a third aspect of the present invention, the application of a copper-based metal complex in an antioxidant reaction is also provided.

[0019] Furthermore, the ability of the copper-based metal complex to scavenge •OH was verified using a Fenton-like reaction; and the ability of the copper-based metal complex to scavenge O2 was verified using an NBT photoreduction reaction. •- The ability.

[0020] The beneficial effects of this invention are as follows: the copper-based metal complex of this invention possesses specific crystal and spatial structures, exhibits high synthetic purity and good stability, and can be used for antioxidant activity. The scavenging of •OH and O2 by H4dhbdc and the copper-based metal complex was investigated under the same conditions. •- In terms of ability, copper-based metal complexes are comparable to H4dhbdc in scavenging •OH free radicals, and also in scavenging O2. •- It performs better in terms of free radicals, indicating that the complexed Cu 2+ It not only retained the antioxidant activity of H4dhbdc, but also enhanced its enzyme activity. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a flowchart of a method for preparing a copper-based metal complex according to an embodiment of the present invention;

[0023] Figure 2 This is a schematic diagram of a copper-based metal complex according to an embodiment of the present invention;

[0024] Figure 3 This is a schematic diagram of the smallest asymmetric unit of a copper-based metal complex according to an embodiment of the present invention;

[0025] Figure 4 This is a schematic diagram of X-ray diffraction powder of a copper-based metal complex according to an embodiment of the present invention;

[0026] Figure 5 Different concentrations of copper-based metal complexes and H4dhbdc for scavenging O2 are described in embodiments of the present invention. •- Situation diagram;

[0027] Figure 6 Different concentrations of copper-based metal complexes and H4dhbdc for scavenging O2 are described in embodiments of the present invention. •- Clearance rate graph;

[0028] Figure 7 This is a schematic diagram illustrating the removal of •OH by H4dhbdc from copper-based metal complexes at different concentrations according to embodiments of the present invention.

[0029] Figure 8 This is a graph showing the scavenging rate of H4dhbdc for •OH at different concentrations in the copper-based metal complex according to embodiments of the present invention. Detailed Implementation

[0030] To further illustrate the various embodiments, the present invention provides accompanying drawings, which are part of the disclosure of the present invention. These drawings are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these drawings, those skilled in the art should be able to understand other possible implementation methods and the advantages of the present invention. The components in the drawings are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0031] According to embodiments of the present invention, a method for preparing copper-based metal complexes and their application in antioxidant activity are provided.

[0032] The present invention will now be further described with reference to the accompanying drawings and specific embodiments. According to an embodiment of the present invention, the copper-based metal complex has the following chemical formula:

[0033] [Cu(H3dhbdc)2(H2O)2], where H3dhbdc is deprotonated 2,3-dihydroxyterephthalic acid.

[0034] In this optional embodiment, the copper-based metal complex has a green rice-grain-like crystal form and a specific crystal structure and spatial structure.

[0035] In this optional embodiment, the crystal structure parameters of the copper-based metal complex include: space group triclinic P-1; unit cell parameters: a = 6.8864(2) Å, b = 6.9466(2) Å, c = 9.6984(3) Å, α = 69.266°, β = 75.085°, γ = 76.367°; and unit cell volume of 413.88(2) Å. 3 The number of asymmetric units Z in the unit cell is 2.

[0036] It should be added that, such as Figure 2 As shown, the copper-based metal complex has a specific crystal structure and spatial structure, and exhibits high synthesis purity and good stability.

[0037] like Figure 1 As shown, according to a second embodiment of the present invention, a method for preparing a copper-based metal complex is also provided, the method comprising the following steps:

[0038] S1. Mix H2O and MeOH to prepare a mixed solvent;

[0039] S2. Cu(NO3)2·3H2O and H4dhbdc are added to the obtained mixed solvent and reacted under preset conditions to obtain green rice-grain-shaped crystals.

[0040] In this optional embodiment, H2O and MeOH are mixed in a molar ratio of 1:1.

[0041] In this optional embodiment, the molar ratio of Cu(NO3)2·3H2O to H4dhbdc is 1:1.

[0042] In this optional embodiment, the preset conditions are:

[0043] The reaction was carried out at a constant temperature of 70℃ for 2 days.

[0044] According to a third embodiment of the present invention, the application of a copper-based metal complex in an antioxidant reaction is also provided.

[0045] In this optional embodiment, a Fenton-like reaction is used to verify the ability of the copper-based metal complex to scavenge •OH; and an NBT photoreduction reaction is used to verify the ability of the copper-based metal complex to scavenge O2. •- The ability.

[0046] It should be added that, such as Figures 3-8 As shown in the figure (Synthesis represents the synthesis of the compound; Simulate represents the structural simulation of the compound; degree represents the angle; Concentration represents the concentration of the compound; scavenging rate represents the scavenging rate), the copper-based metal complex can be used for antioxidant activity. The ability to scavenge •OH is an important indicator for measuring the antioxidant capacity of a substance. After reacting 1 Mm •OH generated by a Fenton-like reaction with materials of different concentrations in the dark for 30 min, the absorbance at 904 nm was measured. The results showed that the scavenging ability of the copper-based metal complex to scavenge •OH showed no statistically significant difference when the concentration was 20 μg / mL, indicating that the scavenging effect was comparable. The O2 scavenging ability of the copper-based metal complex was evaluated using the NBT photoreduction reaction. •- The results indicate its ability to remove O2. •- The ability of the copper-based metal complex to improve with increasing concentration is shown to be better than that of H4dhbdc, indicating that the complexed Cu... 2+ It not only retained the antioxidant activity of H4dhbdc, but also enhanced its enzyme activity.

[0047] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A copper-based metal complex, characterized in that, The chemical formula of the copper-based metal complex is: [Cu(H3dhbdc)2(H2O)2], where H3dhbdc is deprotonated 2,3-dihydroxyterephthalic acid.

2. The copper-based metal complex according to claim 1, characterized in that, The copper-based metal complex has a green, rice-grain-like crystal form and a specific crystal structure and spatial structure.

3. The copper-based metal complex according to claim 2, characterized in that, The crystal structure parameters of the copper-based metal complex include: space group triclinic P-1; unit cell parameters: a = 6.8864(2) Å, b = 6.9466(2) Å, c = 9.6984(3) Å, α = 69.266°, β = 75.085°, γ = 76.367°; and unit cell volume of 413.88(2) Å. 3 The number of asymmetric units Z in the unit cell is 2.

4. A method for preparing a copper-based metal complex, comprising the preparation method of the copper-based metal complex as described in claim 3, characterized in that, The preparation method includes the following steps: S1. Mix H2O and MeOH to prepare a mixed solvent; S2. Cu(NO3)2·3H2O and H4dhbdc are added to the obtained mixed solvent and reacted under preset conditions to obtain green rice-grain-shaped crystals.

5. The method for preparing a copper-based metal complex according to claim 4, characterized in that, The H2O and MeOH are mixed in a molar ratio of 1:

1.

6. The method for preparing a copper-based metal complex according to claim 5, characterized in that, The molar ratio of Cu(NO3)2·3H2O to H4dhbdc is 1:

1.

7. The method for preparing a copper-based metal complex according to claim 6, characterized in that, The preset conditions are: The reaction was carried out at a constant temperature of 70℃ for 2 days.

8. The application of a copper-based metal complex as described in claim 1 in antioxidant active reactions.

9. The application of a copper-based metal complex according to claim 8 in antioxidant reactions, characterized in that, The ability of the copper-based metal complex to scavenge •OH was verified using a Fenton-like reaction; the ability of the copper-based metal complex to scavenge O2 was verified using an NBT photoreduction reaction. •- The ability.

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