Single-sample multi-omics component extraction method for multi-omics conjoint analysis

By employing zinc ion-mediated protein capture and Fe-IMAC phosphorylation enrichment technology, the problem of high salt content and interfering substances coexisting in nucleic acid extraction residues has been solved. This enables efficient multi-omics analysis in a single sample, simultaneously acquiring DNA, RNA, and proteomic data, and is suitable for rare cell populations and clinical biopsy samples.

CN122256333APending Publication Date: 2026-06-23GUANGZHOU NAT LAB +1
6 Cites 0 Cited by

Patent Information

Application Number
CN202610445886.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-07
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing technologies struggle to efficiently extract and analyze multi-omics information from limited samples. In particular, proteomics analysis of residual solutions after nucleic acid extraction is affected by high salt content and interfering substances, leading to protein loss and PTM modification shedding, thus hindering reliable deep proteomics analysis.

Method used

By employing zinc ion-mediated protein capture technology and Fe-IMAC phosphorylation enrichment technology, combined with nucleic acid extraction via centrifugation column method, an integrated multi-omics analysis workflow was constructed. Proteins were precipitated in high-salt effluent using a zinc ion precipitation strategy, and interfering substances were removed using Fe-IMAC enrichment technology, achieving efficient protein capture and mass spectrometry-compatible conversion.

Benefits of technology

Simultaneous acquisition of high-quality DNA, RNA, and proteomic data from a single sample enables in-depth proteomic and phosphorylated proteomic identification, reduces sample requirements and shortens preprocessing time, avoids the use of high-risk organic reagents, and ensures data quality.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a single-sample multi-omics component extraction method for multi-omics conjoint analysis. The method comprises the following steps: adding a nucleic acid extracting solution into a to-be-detected sample, transferring to a DNA adsorption column, centrifuging, and collecting filtrate; washing the DNA adsorption column; eluting the DNA (Deoxyribonucleic Acid); adding ethanol into the filtrate, transferring to an RNA adsorption column, centrifuging, and collecting the filtrate; washing the RNA adsorption column; the RNA is eluted; and extracting protein. According to the method, a DNA / RNA co-extraction technology based on a centrifugal column, a zinc ion mediated protein precipitation capture strategy and an efficient Fe-IMAC phosphorylated peptide fragment enrichment process are integrated, efficient precipitation and in-situ cleaning of the protein are achieved, chemical components interfering mass spectrometric detection are thoroughly removed, and the method is suitable for mass spectrometric detection. Extraction of high-quality nucleic acid and coverage of deep proteome and phosphorylated proteome are synchronously achieved in a single sample, and the bottlenecks of a traditional method in the aspects of limited sample size, complex process, poor reproducibility and the like are effectively solved.
Need to check novelty before this filing date? Find Prior Art