Accurate temperature control cell culture mechanism

Abstract

The utility model discloses an accurate temperature control cell culture mechanism which is characterized by comprising a heating cavity, a temperature control assembly and a heating cover, the heating cavity comprises a cavity body, and a temperature control assembly mounting position for mounting a temperature control assembly is arranged on the cavity body; the heating cover is positioned at the top of the heating cavity; the heating cover comprises a cover plate and a cover plate heating sheet; a temperature sensor and cover plate heat preservation foam are further included. The temperature control assembly comprises a heating sheet, a temperature sensor and a heat dissipation assembly; the temperature control assemblies are symmetrically installed on the heating cavity body. The heating cavity further comprises an infrared temperature detector and cavity heat preservation foam. And the infrared temperature detector is electrically connected with the temperature control assembly and the heating cover. The utility model provides the cell culture mechanism capable of accurately controlling the temperature, which can monitor the internal environment in real time, keep the whole temperature consistent, eliminate condensed water at the top of the culture device and prevent the condensed water from dripping to generate a shearing force effect on cells.

Description

Technical Field

[0001] The utility model relates to the field of cell culture equipment, in particular to a cell culture mechanism with precise temperature control. Background Art

[0002] During cell culture, cell growth, proliferation and metabolic activities are directly regulated by temperature. Temperature is one of the key factors in regulating cell metabolic activities. Appropriate temperature can accelerate cell metabolism and promote material conversion and biosynthesis. However, if the temperature fluctuates too much, it will lead to disorder of cell metabolic activities, thus affecting the growth rate and survival state of cells. A stable temperature environment is essential for the normal growth and survival of cells. Therefore, the temperature should be kept stable at all times during cell culture.

[0003] In the conventional cell culture system of the prior art, a heating device is installed at the bottom or side of the culture container, and a temperature sensor is installed inside the culture chamber to control the temperature of the cell culture process. Due to the limitation of the overall structure of the culture chamber, the temperature control sensor is unevenly arranged, and there is no insulation material outside the heating device, resulting in uneven and inaccurate temperature control. Usually, a heating device is installed at the bottom or side of the culture vessel, which will cause the liquid in the culture vessel to evaporate. The evaporated water is cooled (the temperature of the upper cover is lower than the temperature of the lower liquid, the upper cover is a cold surface, and the water vapor encounters the cold surface) and condenses into a liquid film at the top of the culture vessel. When the water vapor content in the air exceeds the saturation, the water vapor molecules will condense to form water droplets. The condensed water droplets form larger water droplets and then drip back into the culture liquid, which will produce shear force on the cells and cause adverse risks to cell culture.

[0004] Therefore, there is an urgent need for a cell culture mechanism with closed space and precise temperature control that can monitor the internal environment in real time, maintain a consistent overall temperature, and prevent condensation water from dripping from the top. Utility Model Content

[0005] In order to solve the above technical problems, improve the shortcomings of traditional cell culture mechanisms, and optimize the cell culture mechanisms, the specific solutions are as follows:

[0006] A cell culture mechanism with precise temperature control, characterized in that the cell culture mechanism comprises a heating chamber, a temperature control component and a heating cover; the heating chamber comprises a cavity body, and a temperature control component mounting position for mounting the temperature control component is provided on the cavity body; the heating cover is located at the top of the heating chamber; the heating cover comprises a cover plate and a cover plate heating sheet.

[0007] Furthermore, the heating cover also includes a temperature sensor and cover plate thermal insulation foam.

[0008] Preferably, the cover plate thermal insulation foam covers the cover plate, the cover plate heating plate and the temperature sensor are installed in a cavity surrounded by the cover plate and the cover plate thermal insulation foam, and the cover plate heating plate is electrically connected to the temperature sensor.

[0009] Furthermore, the temperature control component includes a heating plate, a temperature sensor, and a heat dissipation component.

[0010] Preferably, the temperature control assembly is symmetrically mounted on the heating chamber body.

[0011] Preferably, one side of the heating plate is fixedly connected to the heating chamber body, and the other side is in contact with the heat dissipation component, and the temperature sensor is fixed on the heat dissipation component.

[0012] Preferably, the symmetrical installation of the temperature control components can achieve uniform heating of the entire heating chamber, and can accurately control the temperature of the heating chamber in real time.

[0013] Furthermore, the temperature sensor also has an overheat warning function. When the temperature sensor detects that the heating plate exceeds the set temperature standard, it will issue an overheat alarm to prevent the high temperature from having adverse effects on the cultured cells.

[0014] Preferably, the heat dissipation component can keep the temperature of the heating plate stable, so that the heating plate can achieve stable heat dissipation. At the same time, the temperature in the heating chamber is maintained at an appropriate temperature for cell culture, preventing the temperature sensor itself from overheating and failing, causing the temperature control component to malfunction.

[0015] Furthermore, the heat dissipation component includes fixedly connected heat dissipation fins, a channel cover plate and a fan. This design has a compact structure, saves installation space, and effectively maintains the normal working temperature of the temperature control component.

[0016] Furthermore, the temperature sensor is fixedly mounted on the heat sink fins; the heat sink fins can effectively transfer heat from the core components to a larger area, thereby improving the heat dissipation efficiency.

[0017] Preferably, the channel cover is fixedly mounted to the side of the heat dissipation fins, and can guide the air to flow along a preset path, ensuring that the air absorbs and takes away as much heat as possible when flowing through the heat dissipation fins, thereby improving the heat dissipation efficiency.

[0018] Preferably, the fan is fixedly installed at one end of the heat dissipation fins, which can speed up the heat exchange rate between the heat dissipation fins and the air and enhance the active heat dissipation capability of the heat dissipation component.

[0019] Furthermore, the temperature control component also includes a foam orifice plate; the foam orifice plate is aligned and fixedly installed on the heat dissipation fins, and a positioning hole for accommodating the heating plate is provided in the middle of the foam orifice plate. The two sides of the heating plate are respectively abutted against the heat dissipation fins and the cavity, and thermal conductive silicone grease is applied on both sides of the heating plate.

[0020] Preferably, the foam porous plate has good thermal insulation performance and can effectively prevent the transfer and loss of heat.

[0021] Furthermore, the heating chamber also includes an infrared thermometer and cavity insulation foam.

[0022] Furthermore, the infrared thermometer is located at the bottom of the cavity and can monitor the temperature of the liquid in the culture device in real time.

[0023] Furthermore, the cavity insulation foam is located outside the cavity, which can effectively insulate and preserve heat.

[0024] Furthermore, the infrared thermometer is electrically connected to the temperature control component and the heating cover.

[0025] Preferably, the infrared thermometer can measure the temperature in the cavity quickly and accurately without contact. When linked with the temperature control component and the heating cover, the temperature information can be obtained in real time to ensure that the heating process is always carried out within the set temperature range.

[0026] Furthermore, the heating cover is installed in cooperation with the heating cavity to form a relatively closed space.

[0027] Furthermore, the cavity and the cover plate are made of metal heat-conducting material, which can transfer heat quickly and effectively.

[0028] The utility model adopts the design of heating chamber, temperature control component and heating cover for cell culture mechanism with precise temperature control. By setting the cover heating plate on the cover plate, and then symmetrically installing the temperature control components on both sides of the heating chamber, and setting the infrared thermometer at the bottom of the heating chamber, the interior is detected in real time to ensure the temperature of the closed space in the chamber is consistent, and the evaporation of water is avoided during the heating process. At the same time, it prevents the evaporated water from condensing on the top of the culture device, and the dripping water drops from exerting shear force on the cells.

[0029] Compared with the prior art, the beneficial effects of the utility model include:

[0030] 1. The utility model provides a cell culture mechanism with precise temperature control, which can uniformly and accurately control the temperature of the cell culture process, and at the same time eliminate condensed water on the top of the culture device, so as to avoid the condensed water dripping back into the culture solution and impacting the cell culture solution surface to generate shear force, thereby causing adverse risks to cell culture.

[0031] 2. The heating cover of the cell culture mechanism with precise temperature control of the utility model has a cover heating plate and a temperature sensor added to the cover, which can effectively eliminate the condensed water on the top of the culture device and prevent the condensed water from forming large water droplets, which will impact the liquid surface when dripping back into the cell culture liquid, and the shear force generated by the impact will cause cell rupture or damage. The heating cover and the heating chamber are installed in conjunction to form a closed space, which effectively maintains the overall temperature consistency.

[0032] 3. The cell culture mechanism with precise temperature control of the present invention is also provided with temperature control components symmetrically installed on both sides of the heating chamber, which can make the heating chamber as a whole evenly heated and precisely control the temperature of the heating chamber in real time.

[0033] 4. The bottom of the heating chamber in the cell culture mechanism with precise temperature control of the utility model is provided with an infrared thermometer, which can detect the cell culture device built into the chamber in real time. The infrared thermometer is electrically connected to the temperature control component and the heating cover, so that multiple groups of temperature sensors can be linked and controlled in real time, making the temperature control in the chamber more precise. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The present invention will be further described below with reference to the accompanying drawings and embodiments, in which:

[0035] Figure 1 An exploded view of the cell culture mechanism with precise temperature control of the utility model;

[0036] Figure 2 This is a schematic diagram of a heating cover for a cell culture mechanism with precise temperature control according to the utility model;

[0037] Figure 3 This is a schematic diagram of the heating chamber of the cell culture mechanism with precise temperature control of the utility model;

[0038] Figure 4 This is a schematic diagram of the temperature control component of the cell culture mechanism with precise temperature control of the utility model;

[0039] Figure 5 This is a schematic diagram of the structure of the cell culture mechanism and culture cup with precise temperature control in Example 1.

[0040] The reference numerals in the figure are as follows: 1-heating cover, 101-cover plate, 102-cover plate heating plate, 103-temperature sensor, 104-cover plate insulation foam, 2-heating chamber, 201-cavity, 202-temperature control component installation position, 203-infrared thermometer, 204-cavity insulation foam, 3-temperature control component, 301-temperature sensor, 302-heating plate, 303-foam orifice plate, 304-heating fins, 305-fan, 306-channel cover, 4-culture cup. DETAILED DESCRIPTION

[0041] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. The exemplary embodiments and descriptions of the present invention are used to explain the present invention and do not constitute improper limitations on the present invention. It should be noted that the embodiments and features in the embodiments of the present invention can be combined with each other without conflict.

[0042] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate directions or positional relationships based on the attached Figure 1 The orientation or positional relationship shown is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the utility model, unless otherwise specified, "multiple" means two or more.

[0043] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0044] refer to Figure 1-4 The utility model provides a cell culture mechanism with precise temperature control, characterized in that the cell culture mechanism comprises a heating chamber 2, a temperature control component 3 and a heating cover 1; the heating chamber 2 comprises a cavity 201, and a temperature control component mounting position 202 is provided on the cavity 201; the heating cover 1 is located at the top of the heating chamber 2; the heating cover 1 comprises a cover plate 101, a cover plate heating sheet 102, a temperature sensor 103 and a cover plate thermal insulation foam 104; the cover plate thermal insulation foam 104 is covered on the cover plate 101, the cover plate heating sheet 102 and the temperature sensor 103 are installed in the cavity surrounded by the cover plate 101 and the cover plate thermal insulation foam 104, and the cover plate heating sheet 102 is electrically connected to the temperature sensor 103.

[0045] The temperature control component 3 includes a heating plate 302, a temperature sensor 301, a heat dissipation component and a foam orifice plate 303. The temperature control component 3 is symmetrically mounted on the temperature control component mounting position 202 of the heating chamber body 201. The heat dissipation component includes a fixedly connected heat dissipation fin 304, a channel cover plate 306 and a fan 305. The temperature sensor 301 is fixedly mounted on the heat dissipation fin 304; the foam orifice plate 303 is aligned and fixedly mounted on the heat dissipation fin 304, the heating plate 302 is located in the middle of the foam orifice plate 303 and is provided with a positioning hole for accommodating the heating plate, the two sides of the heating plate 302 are respectively abutted against the heat dissipation fin 304 and the cavity, and both sides of the heating plate 302 are coated with thermal conductive silicone grease.

[0046] The heating chamber 2 further includes an infrared thermometer 203 and a cavity insulation foam 204. The infrared thermometer 203 is located at the bottom of the cavity 201, and the cavity insulation foam 204 is located outside the cavity 201. The infrared thermometer 203 is electrically connected to the temperature control component 3 and the heating cover 1. The cavity 201 and the cover plate 101 are made of metal heat conductive material.

[0047] In order to better understand the technical solution of the utility model, the following specific embodiments are selected to illustrate the installation and use method of the cell culture mechanism with precise temperature control of the utility model. In view of the problems related to the temperature control of cell culture, in combination with patent CN202320467188, a cell culture device with perfusion function is disclosed, which is used in conjunction with the precise temperature control cell culture mechanism of the utility model. (The interior of the cavity fits the shape of the culture cup, and the heat of the temperature control component can be quickly transferred to the entire cavity, and the bottom and surrounding of the culture cup are fully heated and insulated) Figure 5 shown.

[0048] Example 1

[0049] 1) First, install the temperature control component, and install the heat sink fin 304, the channel cover 306 and the fan 305 in sequence. The channel cover 306 is located on the side of the heat sink fin 304, and the fan 305 is fixedly installed on one end of the heat sink fin 304. Then fix the whole, and then fix the temperature sensor 301 on the heat sink fin 304, and then align the foam orifice plate 303 and fix it on the heat sink fin 304. Apply thermal grease evenly on both sides of the heating plate 302. Finally, install the heating plate 302 coated with thermal grease in the middle positioning hole of the foam orifice plate 303, and the two sides of the heating plate 302 are respectively in contact with the heat sink fin 304 and the cavity.

[0050] 2) Then install the temperature control assembly 3 on the heating chamber 2. The two sets of temperature control assemblies 3 are symmetrically installed on the temperature control assembly installation positions 202 on the side of the heating chamber 2, and then fixed.

[0051] 3) Then, the heating cover 1 is installed, and the temperature sensor 103 and the cover heating plate 102 are fixedly installed on the cover plate 101 in sequence, and finally, the cover insulation foam 104 set is covered on the cover plate 101 .

[0052] 4) The culture cup drive device injected with cell culture fluid and cells is adsorbed onto the culture cup cover, the culture cover is covered and the sealing is checked. The interior of the cavity 201 of the heating chamber 2 is adapted to the shape of the culture cup. Then the prepared culture cup 4 is assembled with the cavity 201 and then fixed.

[0053] 5) Finally, the cover plate 101 of the heating cover 1 is installed in cooperation with the heating chamber 2 to ensure that the space where the culture cup 4 is located is closed.

[0054] 6) According to the temperature requirement of cell culture, the bottom and surrounding of the culture cup 4 are fully heated and insulated, and the infrared temperature detector 203 at the bottom of the heating chamber 2 detects the liquid inside the culture cup in real time, and is linked with the temperature control component and the heating cover for control.

[0055] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structural transformation made using the contents of the present invention specification, or directly or indirectly applied in other related technical fields, is also included in the patent protection scope of the present invention.

Claims

1. A cell culture mechanism with precise temperature control, characterized in that: The cell culture mechanism comprises a heating chamber, a temperature control component and a heating cover; The heating chamber comprises a chamber body, on which a temperature control component mounting position for mounting the temperature control component is arranged; the heating cover is located at the top of the heating chamber; the heating cover comprises a cover plate and a cover plate heating sheet.

2. The cell culture mechanism according to claim 1, characterized in that: The heating cover also includes a temperature sensor and a cover plate thermal insulation foam.

3. The cell culture mechanism according to claim 1, characterized in that: The temperature control component includes a heating plate, a temperature sensor, and a heat dissipation component.

4. The cell culture mechanism according to claim 3, characterized in that: The heat dissipation assembly includes heat dissipation fins, a channel cover plate and a fan which are fixedly connected.

5. The cell culture mechanism according to claim 3, characterized in that: The temperature sensor is fixedly mounted on the heat dissipation fins.

6. The cell culture mechanism according to claim 3, characterized in that: The temperature control component also includes a foam orifice plate; the foam orifice plate is aligned and fixedly installed on the heat dissipation fins, and a positioning hole for accommodating the heating plate is provided in the middle of the foam orifice plate. The two sides of the heating plate are respectively abutted against the heat dissipation fins and the cavity, and thermal conductive silicone grease is applied on both sides of the heating plate.

7. The cell culture mechanism according to claim 1, characterized in that: The heating cavity also includes an infrared temperature detector and cavity thermal insulation foam.

8. The cell culture mechanism according to claim 7, characterized in that: The infrared thermometer is located at the bottom of the cavity, and the cavity insulation foam is located outside the cavity.

9. The cell culture mechanism according to claim 7, characterized in that: The infrared temperature detector is electrically connected to the temperature control component and the heating cover.

10. The cell culture mechanism according to any one of claims 1 to 9, characterized in that: The cavity and the cover plate are made of metal heat-conducting material.

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