Novel protein gel filtration chromatography column

Abstract

The utility model relates to the technical field of gel filtration chromatography, in particular to a novel protein gel filtration chromatography column which comprises a filtration chromatography column body, a protection component, a feeding component and a filtrate guide outlet, the feeding component comprises a feeding hopper, a supporting rod is arranged on the inner side of the feeding hopper, a flow guide block is arranged on the lower side of the supporting rod, and the filtrate guide outlet is arranged on the lower side of the supporting rod. A discharging port is formed in the lower end of the feeding hopper, a rubber plug is arranged at the lower end of the outer side of the feeding hopper, a mounting frame is arranged on the outer side of the feeding hopper, a first sliding rod is arranged at the lower end of the mounting frame, and a first clamping ring is arranged on one side of the first sliding rod. According to the utility model, the material is guided through the feeding component, so that the material slides to the lower end of the filtering chromatographic column main body through the inner wall of the filtering chromatographic column main body to finish feeding, the collision among the materials is reduced, the generation of bubbles is reduced, the filtering chromatographic column main body is protected through the protection component, and the use safety is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of gel filtration chromatography, in particular to a novel protein gel filtration chromatography column. Background Art

[0002] Gel filtration chromatography is a technique that utilizes the molecular sieving effect of particles with a porous network structure to elute and separate based on the differences in relative molecular weights of the components in the sample being separated. Gel filtration chromatography, also known as size exclusion chromatography or molecular sieve method, is mainly used to separate and purify proteins based on their size and shape, that is, their mass.

[0003] A Chinese patent discloses a novel protein gel filtration chromatography column (authorization announcement number CN219630672U). The patented technology is provided with a filtering and draining mechanism. During use, by turning the adjusting knob, the adjusting knob drives the rotating cylinder to rotate inside the fixed cylinder, thereby draining the liquid, filtering the dripping liquid through the filter screen, and when replacing the filter screen, only the rotating block needs to be rotated to separate the rotating block from the block, and then the filter screen can be pulled out to replace the filter screen, which saves trouble and labor. However, during the introduction of the materials of the gel filtration chromatography column, the collision force between the materials is large, which will produce a large number of bubbles, affecting the subsequent filtration efficiency, and the column of the filtration chromatography column is exposed to the outside, which is easy to break under the action of air pressure, and there is a safety hazard. Therefore, those skilled in the art provide a novel protein gel filtration chromatography column to solve the problems raised in the above-mentioned background technology. Utility Model Content

[0004] The utility model aims to provide a novel protein gel filtration chromatography column to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A novel protein gel filtration chromatography column comprises a filtration chromatography column body, a protective component, a feed component and a filtrate outlet, wherein the protective component is located at the outer side of the filtration chromatography column body, the feed component is located at the upper end of the filtration chromatography column body, and the filtrate outlet is located at the lower end of the filtration chromatography column body. The feed component comprises a feeding hopper, a support rod is provided on the inner side of the feeding hopper, a guide block is provided on the lower side of the support rod, a discharge port is provided at the lower end of the feeding hopper, a rubber plug is provided at the lower end of the outer side of the feeding hopper, a mounting frame is provided at the outer side of the feeding hopper, a first sliding rod is provided at the lower end of the mounting frame, a first clamping ring is provided on one side of the first sliding rod, and a first spring is installed at one end of the outer side of the first sliding rod.

[0007] As a further solution of the utility model: the protective component includes a protective net, rings are provided at both ends of the protective net, a second sliding rod is provided at the outer end of the ring, a second clamping ring is provided on one side of the second sliding rod, and a second spring is installed at one end of the outer side of the second sliding rod.

[0008] As a further solution of the utility model: the lower end of the hopper is connected to the upper end of the filtration chromatography column body through the discharge port, and the lower end of the filtration chromatography column body is connected to the upper end of the filtrate outlet.

[0009] As a further solution of the utility model: the upper end of the support rod is fixed to the inner side of the feeding hopper, the outer end of the first slide rod slides to the lower end of the mounting frame, and the first clamping ring slides to the inner side of the mounting frame through the first slide rod.

[0010] As a further solution of the utility model: the rubber plug is located at the inner side of the filtration chromatography column body, and the feeding hopper is engaged with the inner side of the filtration chromatography column body through the rubber plug.

[0011] As a further solution of the utility model: the outer end of the second sliding rod slides at the outer end position of the collar, and the second clamping ring slides at the inner side position of the collar through the second sliding rod.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The utility model is a novel protein gel filtration chromatography column that guides the introduced material through the feeding component, so that the material slides through the inner wall of the filtration chromatography column body to the lower end of the filtration chromatography column body to complete the feeding, thereby reducing the collision between the materials and the generation of bubbles, which is beneficial to the filtration efficiency of the filtration chromatography column;

[0014] 2. The filter chromatography column body is protected by the protective component to improve the safety of use, and the filter chromatography column body and the protective component are detachable structures for easy installation. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the structure of a new type of protein gel filtration chromatography column;

[0016] Figure 2 It is a schematic diagram of the structure of a feed component in a novel protein gel filtration chromatography column;

[0017] Figure 3 This is a schematic diagram of the structure of a protective component in a new type of protein gel filtration chromatography column.

[0018] In the figure: 1. filter chromatography column body; 2. protective component; 3. feed component; 4. filtrate outlet; 5. feeding hopper; 6. support rod; 7. guide block; 8. discharge port; 9. rubber plug; 10. mounting frame; 11. first slide bar; 12. first spring; 13. first clamping ring; 14. protective net; 15. collar; 16. second slide bar; 17. second clamping ring; 18. second spring. DETAILED DESCRIPTION

[0019] See also Figures 1 to 3 In the embodiment of the utility model, a novel protein gel filtration chromatography column comprises a filtration chromatography column body 1, a protective component 2, a feed component 3 and a filtrate outlet 4, wherein the protective component 2 is located at the outer side of the filtration chromatography column body 1, the feed component 3 is located at the upper end of the filtration chromatography column body 1, the filtrate outlet 4 is located at the lower end of the filtration chromatography column body 1, the feed component 3 comprises a hopper 5, a support rod 6 is arranged on the inner side of the hopper 5, a guide block 7 is arranged on the lower side of the support rod 6, and a guide block 7 is arranged on the lower side of the hopper 5. A discharge port 8 is provided at the end, a rubber stopper 9 is provided at the lower end of the outer side of the feeding hopper 5, a mounting frame 10 is provided at the outer side of the feeding hopper 5, a first slide bar 11 is provided at the lower end of the mounting frame 10, a first clamping ring 13 is provided on one side of the first slide bar 11, a first spring 12 is installed at one end of the outer side of the first slide bar 11, the lower end of the feeding hopper 5 is connected with the upper end of the filtration chromatography column body 1 through the discharge port 8, the lower end of the filtration chromatography column body 1 is connected with the upper end of the filtrate outlet 4, and the upper end of the support rod 6 is fixed to At the inner side of the hopper 5, the outer end of the first slide bar 11 slides at the lower end of the mounting frame 10, the first clamping ring 13 slides at the inner side of the mounting frame 10 through the first slide bar 11, the rubber plug 9 is located at the inner side of the filtration chromatography column body 1, and the hopper 5 is engaged with the inner side of the filtration chromatography column body 1 through the rubber plug 9. First, a new type of protein gel filtration chromatography column is carried to the use position, the protective component 2 is put on the outer side of the filtration chromatography column body 1, and the guide block 7 is inserted into the filtration chromatography column body. The inner side of the body 1, the rubber stopper 9 is stuck to the upper end of the filtration chromatography column body 1, the first spring 12 rebounds, the outer end of the first slide rod 11 slides on the lower end of the mounting frame 10, and the first clamping ring 13 is pressed to the outer side of the filtration chromatography column body 1 to complete the installation of the feeding component 3, and then, the material is poured into the inside of the feeding hopper 5, and the material inside the feeding hopper 5 is discharged through the discharge port 8, the guide block 7 guides the material to the inner wall of the filtration chromatography column body 1, the material slides into the lower end of the filtration chromatography column body 1, and the feeding component 3 is removed for filtering.

[0020] exist Figure 1 , 3In the figure: the protective component 2 includes a protective net 14, and a ring 15 is provided at both ends of the protective net 14. A second slide bar 16 is provided at the outer end of the ring 15, and a second clamping ring 17 is provided on one side of the second slide bar 16. A second spring 18 is installed at one end of the outer side of the second slide bar 16. The outer end of the second slide bar 16 slides at the outer end position of the ring 15, and the second clamping ring 17 slides at the inner side position of the ring 15 through the second slide bar 16. The protective component 2 is sleeved onto the outer side of the filtration chromatography column body 1, and the second spring 18 rebounds so that the outer end of the second slide bar 16 slides at the outer end of the ring 15, and the second clamping ring 17 is pressed to the outer side of the filtration chromatography column body 1 to fix the protective component 2.

[0021] The working principle of the utility model is as follows: first, a novel protein gel filtration chromatography column is carried to the use position, the protective component 2 is sleeved onto the outer side of the filtration chromatography column body 1, the second spring 18 rebounds the outer end of the second slide bar 16 to slide on the outer end of the sleeve ring 15, the second clamping ring 17 is pressed onto the outer side of the filtration chromatography column body 1 to fix the protective component 2, the guide block 7 is inserted into the inner side of the filtration chromatography column body 1, the rubber plug 9 is clamped onto the upper end of the filtration chromatography column body 1, the first spring 12 rebounds the outer end of the first slide bar 11 to slide on the lower end of the mounting frame 10, the first clamping ring 13 is pressed onto the outer side of the filtration chromatography column body 1, the installation of the feeding component 3 is completed, and then, the material is poured into the inside of the feeding hopper 5, the material inside the feeding hopper 5 is discharged through the discharge port 8, the guide block 7 guides the material to the inner wall of the filtration chromatography column body 1, the material slides into the lower end of the filtration chromatography column body 1, and the feeding component 3 is removed for filtering.

[0022] What is described above is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A novel protein gel filtration chromatography column, comprising a filtration chromatography column body (1), a protective component (2), a feed component (3) and a filtrate outlet (4), wherein the protective component (2) is located at the outer side of the filtration chromatography column body (1), the feed component (3) is located at the upper end of the filtration chromatography column body (1), and the filtrate outlet (4) is located at the lower end of the filtration chromatography column body (1), characterized in that: The feeding component (3) comprises a hopper (5), a support rod (6) is arranged on the inner side of the hopper (5), a guide block (7) is arranged on the lower side of the support rod (6), a discharge port (8) is arranged at the lower end of the hopper (5), a rubber plug (9) is arranged at the lower end of the outer side of the hopper (5), a mounting frame (10) is arranged on the outer side of the hopper (5), a first slide bar (11) is arranged at the lower end of the mounting frame (10), a first retaining ring (13) is arranged on one side of the first slide bar (11), and a first spring (12) is installed at one end of the outer side of the first slide bar (11).

2. A novel protein gel filtration chromatography column according to claim 1, characterized in that: The protective component (2) comprises a protective net (14), wherein collars (15) are provided at both ends of the protective net (14), a second slide bar (16) is provided at the outer end of the collar (15), a second clamping ring (17) is provided on one side of the second slide bar (16), and a second spring (18) is installed at one end of the outer side of the second slide bar (16).

3. A novel protein gel filtration chromatography column according to claim 1, characterized in that: The lower end of the feeding hopper (5) is connected to the upper end of the filtration chromatography column body (1) through the discharge port (8), and the lower end of the filtration chromatography column body (1) is connected to the upper end of the filtrate outlet (4).

4. A novel protein gel filtration chromatography column according to claim 1, characterized in that: The upper end of the support rod (6) is fixed to the inner side of the hopper (5), the outer end of the first slide rod (11) slides to the lower end of the mounting frame (10), and the first clamping ring (13) slides to the inner side of the mounting frame (10) through the first slide rod (11).

5. A novel protein gel filtration chromatography column according to claim 1, characterized in that: The rubber stopper (9) is located at an inner side of the filtration chromatography column body (1), and the feeding hopper (5) is engaged with the inner side of the filtration chromatography column body (1) through the rubber stopper (9).

6. A novel protein gel filtration chromatography column according to claim 2, characterized in that: The outer end of the second slide bar (16) slides at the outer end position of the collar (15), and the second clamping ring (17) slides at the inner side position of the collar (15) through the second slide bar (16).

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