Self-cleaning anti-blocking cell disruption filtration device

Abstract

This utility model relates to the field of cell disruption and filtration technology, and more particularly to a self-cleaning, anti-clogging cell disruption and filtration device. The technical solution is as follows: A self-cleaning, anti-clogging cell disruption and filtration device includes a shell, a collection frame, a fixed ring plate, a connecting shaft, and a fixed shell; the collection frame is slidably placed at the lower part of the shell, the fixed ring plate is fixedly connected inside the shell, the connecting shaft is rotatably connected to the upper part of the fixed ring plate, and the fixed shell is fixedly connected to the left side of the shell. This utility model performs preliminary filtration through a filter membrane, trapping larger fragments, while small particles and liquid permeate through the filter membrane into the collection frame. A motor drives the connecting shaft to rotate, causing the filter membrane to rotate slowly, evenly distributing cell fragments and avoiding localized accumulation. When the ultrasonic generator is activated, the high-frequency electrical signal is converted into mechanical vibration, and the resulting "cavitation effect" not only effectively breaks down the cell membrane but also releases the cell contents.

Description

Technical Field

[0001] This utility model relates to the field of cell disruption and filtration technology, and in particular to a self-cleaning, anti-clogging cell disruption and filtration device. Background Technology

[0002] The cell is the basic unit of life, constituting the fundamental structure and function of all organisms. Whether single-celled organisms like bacteria and yeast, or multicellular organisms like plants, animals, and humans, all are composed of one or more cells. Cells contain genetic material, DNA, which guides cell function and reproduction, and regulates various life activities through a complex signal transduction network. In some cases, to ensure that reagents can fully contact and react with target substances within the cell (such as PCR amplification and enzyme digestion), the cell must first be disrupted to expose its internal components. This disruption leaves behind a large amount of cell debris, including membrane lipids, unruptured organelles, and other impurities. Filtration effectively removes these insoluble particulate matter.

[0003] Cell lysis produces a large amount of cell debris, including cell membranes, organelles (such as mitochondria and endoplasmic reticulum), and incompletely dissolved nuclei. These larger particles easily accumulate on the filter membrane surface, forming a physical barrier that hinders fluid passage. Furthermore, with cell lysis, large amounts of intracellular high-molecular-weight substances (such as DNA, RNA, and protein complexes) are released. Because these macromolecules are highly viscous, they easily entangle and aggregate on the filter membrane surface, forming a gel-like structure. This gel-like structure not only increases the resistance to fluid passage through the filter membrane but also further exacerbates membrane clogging, thus significantly reducing filtration efficiency.

[0004] Therefore, it is necessary to design a self-cleaning, anti-clogging cell disruption filter to solve the above-mentioned technical problems. Utility Model Content

[0005] In order to overcome the shortcomings of cell fragments and high molecular weight substances generated after cell breakage easily clogging the filter membrane, hindering the passage of liquid, and reducing filtration efficiency, this utility model provides a self-cleaning, anti-clogging cell breakage filtration device.

[0006] The technical solution is as follows: A self-cleaning, anti-clogging cell disruption and filtration device includes a housing, a collection frame, a fixed ring plate, a connecting shaft, a fixed shell, a motor, a transmission wheel, a belt, a filter membrane, a sealing cover, a handle, an ultrasonic generator, an ultrasonic transducer, and an anti-clogging component. A collection frame is slidably placed at the bottom of the housing. A fixed ring plate is fixedly connected inside the housing. A connecting shaft is rotatably connected to the upper part of the fixed ring plate. A fixed shell is fixedly connected to the left side of the housing. A motor is fixedly connected to the bottom of the fixed shell. Transmission wheels are fixedly connected to both the motor's output shaft and the connecting shaft. The two transmission wheels are located inside the fixed shell, and a belt is wound between the two transmission wheels. A filter membrane is installed at the top of the connecting shaft and is located inside the housing. A sealing cover is threadedly connected to the top of the housing. A handle is fixedly connected to the top of the sealing cover. An ultrasonic generator is fixedly connected to the middle of the top of the sealing cover. An ultrasonic transducer is fixedly connected to the bottom of the sealing cover. The ultrasonic transducer extends into the filter membrane. An anti-clogging component is provided on the right side of the sealing cover.

[0007] Preferably, the casing is made of a transparent material.

[0008] Preferably, the bottom structure of the filter membrane is conical.

[0009] Preferably, the anti-clogging component includes a discharge pipe, a scraper, a pump body, and a first connector. The discharge pipe is fixedly connected to the right side of the sealing cover. The lower end of the discharge pipe is connected to and communicates with a scraper. The scraper has multiple feed ports vertically distributed and abuts against the inner wall of the filter membrane. The pump body is connected to and communicates with the discharge pipe. The right side of the discharge pipe is connected to and communicates with the first connector.

[0010] Preferably, the device also includes a high-pressure nozzle, a water supply pipe, a valve, and a second connector. The high-pressure nozzle is fixedly mounted on the right side of the housing. The right side of the high-pressure nozzle is connected to and communicates with the water supply pipe. The right side of the water supply pipe is connected to and communicates with the valve. The right side of the valve is connected to and communicates with the second connector.

[0011] Preferably, the filter also includes a rotating shaft and a cleaning brush, wherein the rotating shaft is fixedly connected to the top of one of the drive wheels, and the cleaning brush is fixedly connected to the rotating shaft, and the cleaning brush abuts against the outer wall of the filter membrane.

[0012] The beneficial effects of this utility model are as follows: 1. This utility model performs preliminary filtration through a filter membrane, where larger fragments are trapped, while small particles and liquids pass through the filter membrane and flow into the collection frame. The motor drives the connecting shaft to rotate, causing the filter membrane to rotate slowly and evenly distribute cell fragments, avoiding local accumulation and reducing the risk of blockage. When the ultrasonic generator is activated, the high-frequency electrical signal is converted into mechanical vibration, and the resulting "cavitation effect" not only effectively breaks the cell membrane and releases the cell contents, but also loosens the macromolecular substances attached to the surface of the filter membrane, preventing the formation of a gel-like structure and ensuring that the entire surface of the filter membrane is thoroughly cleaned.

[0013] 2. This utility model achieves dynamic scraping during cell disruption and filtration by closely cooperating with the scraper and the inner wall of the filter membrane. As the filter membrane rotates, the scraper continuously scrapes off impurities such as cell debris and protein complexes attached to the surface, preventing them from accumulating and forming a physical barrier or gel layer. The scraped-off impurities enter the interior through the feed port on the scraper, and are drawn into the discharge pipe by the pump body. Finally, they are discharged from the device through the waste pipe connected to the first connector, effectively removing the source of blockage, improving filtration efficiency and throughput stability, and reducing the frequency of manual maintenance.

[0014] 3. This utility model achieves efficient rinsing of the outer surface of the filter membrane by connecting the second connector to a high-pressure water source and coordinating with the water supply pipe, valve and high-pressure nozzle. This improves the cleaning efficiency of the filter membrane, prevents clogging, extends the service life of the filter membrane, and also improves the level of automation and operational stability.

[0015] 4. This utility model uses a motor to drive the transmission wheel to rotate, which in turn drives the rotating shaft and cleaning brush to rotate synchronously. During the cell disruption and filtration process, the outer wall of the filter membrane is dynamically brushed. The cleaning brush is made of soft, corrosion-resistant material and adheres closely to the surface of the filter membrane to continuously scrape off attached cell fragments, macromolecular gel layers and other impurities, preventing them from accumulating and forming a clogging layer. This effectively maintains the permeability of the filter membrane, avoids damage to the filter membrane, and extends the service life of the filter membrane. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0017] Figure 2 This is a three-dimensional structural diagram of the shell, collection frame, and fixing ring plate of this utility model.

[0018] Figure 3 This is a three-dimensional structural diagram of the components of this utility model, including the fixed shell, motor, and transmission wheel.

[0019] Figure 4 This is an exploded structural diagram of the sealing cap, handle, and ultrasonic generator components of this utility model.

[0020] Figure 5 This is a three-dimensional structural diagram of the components of this utility model, including the discharge pipe, scraper, and pump body.

[0021] Figure 6 This is a three-dimensional structural diagram of the water supply pipe, high-pressure nozzle, valve, and other components of this utility model.

[0022] Figure 7 This is a three-dimensional structural diagram of the housing, rotating shaft, and cleaning brush of this utility model.

[0023] Explanation of reference numerals in the attached drawings: 1_Housing, 2_Collection frame, 3_Fixing ring plate, 4_Connecting shaft, 5_Fixing shell, 6_Motor, 7_Drive wheel, 8_Belt, 9_Filter membrane, 10_Sealing cover, 11_Handle, 12_Ultrasonic generator, 13_Ultrasonic transducer, 14_Discharge pipe, 1401_Scraper, 15_Pump body, 16_First connector, 17_Water supply pipe, 18_High-pressure nozzle, 19_Valve, 20_Second connector, 21_Rotating shaft, 22_Cleaning brush. Detailed Implementation

[0024] Example: A self-cleaning, anti-clogging cell disruption filter device, such as... Figure 1-7 As shown, the system includes a housing 1, a collection frame 2, a fixing ring plate 3, a connecting shaft 4, a fixed shell 5, a motor 6, a transmission wheel 7, a belt 8, a filter membrane 9, a sealing cover 10, a handle 11, an ultrasonic generator 12, an ultrasonic transducer 13, a discharge pipe 14, a scraper 1401, a pump body 15, and a first connector 16. The housing 1 is made of transparent material, allowing observation of cell disruption and filtration inside the housing. The collection frame 2 is slidably placed on the lower front side of the housing 1. The fixing ring plate 3 is screwed onto the lower side of the inside of the housing 1. The connecting shaft 4 is rotatably connected to the upper side of the inside of the fixing ring plate 3. The fixed shell 5 is screwed onto the left side of the inside of the housing 1. The motor 6 is screwed onto the bottom of the fixed shell 5. The output shaft of the motor 6 and the connecting shaft 4 are both connected to transmission wheels 7 via couplings. The two transmission wheels 7 are located inside the fixed shell 5, and a belt 8 is wound between the two transmission wheels 7. The connecting shaft 4... A filter membrane 9 is installed at the top. The bottom structure of the filter membrane 9 is conical to reduce pressure loss and filtration efficiency reduction caused by sediment accumulation. The filter membrane 9 is located inside the middle of the housing 1. A sealing cover 10 is threadedly connected to the top of the housing 1. A handle 11 is welded to the top of the sealing cover 10. An ultrasonic generator 12 is installed in the middle of the top of the sealing cover 10 by screws. An ultrasonic transducer 13 is installed in the bottom of the sealing cover 10 by screws. The ultrasonic transducer 13 extends into the filter membrane 9. A discharge pipe 14 is installed through the right side of the sealing cover 10 by screws. A scraper 1401 is connected to and communicates with the lower end of the discharge pipe 14. The scraper 1401 has multiple vertically distributed material ports, which are connected to the discharge pipe 14. The scraper 1401 abuts against the inner wall of the filter membrane 9. A pump body 15 is connected to and communicates with the middle of the discharge pipe 14. A first connector 16 is connected to and communicates with the right end of the discharge pipe 14.

[0025] First, rotate the sealing cap 10 clockwise to open it, ensuring the filter membrane 9 is correctly installed. Initially, add the cell suspension to be treated into the filter membrane 9. Then, rotate the sealing cap 10 counterclockwise. At this point, the ultrasonic transducer 13 extends into the filter membrane 9, and the liquid undergoes preliminary filtration through the membrane. Larger cell fragments are trapped on the surface of the membrane 9, while smaller particles and liquid flow through the membrane into the collection frame 2 below. After the motor 6 starts, it drives the connecting... The shaft 4 rotates, causing the filter membrane 9 to rotate slowly. This rotational motion helps to evenly distribute cell debris, avoiding localized accumulation and reducing the risk of clogging. When cell disruption or clogging prevention is needed, the ultrasonic generator 12 activates, generating high-frequency electrical signals. These signals are transmitted to the ultrasonic transducer 13, which converts them into high-frequency mechanical vibrations. These vibrations act directly on the surface of the filter membrane 9, generating microbubbles and inducing a cavitation effect. The microbubbles burst, producing strong shock waves that effectively break down cell membranes, releasing cell contents and loosening macromolecules attached to the surface of the filter membrane 9, preventing the formation of a gel-like structure. As the connecting shaft 4 continues to rotate, the ultrasonic transducer 13 continuously applies vibrations to different areas of the filter membrane 9, ensuring that the entire surface of the filter membrane 9 is effectively cleaned and preventing localized blockage due to long-term accumulation of macromolecules. After a period of treatment, the motor 6 and the ultrasonic generator 12 are turned off, the sealing cover 10 is opened clockwise, and the filter membrane 9 and the collection frame 2 are removed for cleaning or replacement. Membrane 9 is ready for the next round of operation. During cell disruption and filtration, the scraper 1401 remains in close contact with the inner wall of the filter membrane 9. As the filter membrane 9 rotates, the scraper 1401 scrapes off cell debris, protein complexes, and other substances from the surface of the filter membrane 9. The scraped-off impurities enter the interior of the scraper 1401 through the feed port, and are then pumped into the scraper 1401 by activating the pump body 15. The impurities are then discharged through the discharge pipe 14. The first connector 16 is connected to the waste pipe, so the impurities are discharged outside the device. After discharge, the pump body 15 is turned off, which effectively prevents the formation of a physical barrier or gel layer on the surface of the filter membrane 9 and maintains a high filtration efficiency.

[0026] It also includes a high-pressure nozzle 18, a water supply pipe 17, a valve 19, and a second connector 20. The high-pressure nozzle 18 is installed on the right side of the housing 1 by screws. The right side of the high-pressure nozzle 18 is connected to and connected to the water supply pipe 17. The right end of the water supply pipe 17 is connected to and connected to the valve 19. The right end of the valve 19 is connected to and connected to the second connector 20.

[0027] Connect the second connector 20 to the high-pressure water source, open the valve 19 to allow high-pressure water to flow into the water supply pipe 17 and be delivered to the high-pressure nozzle 18. The high-pressure nozzle 18 sprays water evenly onto the outer surface of the filter membrane 9 in a jet form. The high-pressure water flow has a strong impact force and can effectively remove contaminants such as cell debris and protein gel layer attached to the outer wall of the filter membrane 9. The impurities washed off fall to the bottom of the filter membrane 9 with the water flow. After rinsing is completed, close the valve 19, disconnect the second connector 20, and restore the normal filtration process.

[0028] It also includes a rotating shaft 21 and a cleaning brush 22. The rotating shaft 21 is welded to the top of one of the drive wheels 7, and the cleaning brush 22 is glued to the rotating shaft 21. The cleaning brush 22 abuts against the outer wall of the filter membrane 9.

[0029] During the cell disruption and filtration process, the output shaft of the motor 6 continuously drives the transmission wheel 7 to rotate; the rotating shaft 21 linked with it drives the cleaning brush 22 to rotate and scrape off impurities such as cell debris and macromolecular gel layer attached to the outer wall of the filter membrane 9, preventing them from accumulating and forming a blockage layer. The cleaning brush 22 is made of soft corrosion-resistant material, which can effectively remove dirt without damaging the filter membrane 9.

Claims

1. A self-cleaning anti-clogging cell disruption filtration device, characterized in that, The assembly includes a housing (1), a collection frame (2), a fixing ring plate (3), a connecting shaft (4), a fixing shell (5), a motor (6), a drive wheel (7), a belt (8), a filter membrane (9), a sealing cover (10), a handle (11), an ultrasonic generator (12), an ultrasonic transducer (13), and an anti-clogging component. The collection frame (2) is slidably placed at the bottom of the housing (1). The fixing ring plate (3) is fixedly connected inside the housing (1). The connecting shaft (4) is rotatably connected to the upper part of the fixing ring plate (3). The fixing shell (5) is fixedly connected to the left side of the housing (1). The motor (6) is fixedly connected to the bottom of the fixing shell (5). The output shaft of the motor (6) and the connecting shaft are connected to the connecting shaft. A drive wheel (7) is fixedly connected to the shaft (4). The two drive wheels (7) are located inside the fixed shell (5). A belt (8) is wound between the two drive wheels (7). A filter membrane (9) is provided on the top of the connecting shaft (4). The filter membrane (9) is located inside the shell (1). A sealing cover (10) is threadedly connected to the top of the shell (1). A handle (11) is fixedly connected to the top of the sealing cover (10). An ultrasonic generator (12) is fixedly connected to the middle of the top of the sealing cover (10). An ultrasonic transducer (13) is fixedly connected to the bottom of the sealing cover (10). The ultrasonic transducer (13) extends into the filter membrane (9). An anti-clogging component is provided on the right side of the sealing cover (10).

2. A self-cleaning anti-clogging cell disruption filtration device according to claim 1, wherein, The shell (1) is made of transparent material.

3. The self-cleaning anti-clogging cell disruption and filtration device of claim 1, wherein, The bottom structure of the filter membrane (9) is conical.

4. The self-cleaning anti-clogging cell disruption and filtration device of claim 1, wherein, The anti-clogging component includes a discharge pipe (14), a scraper (1401), a pump body (15), and a first connector (16). The right side of the sealing cover (10) is fixedly connected to the discharge pipe (14). The lower end of the discharge pipe (14) is connected to and communicates with the scraper (1401). The scraper (1401) has multiple material ports vertically distributed. The scraper (1401) abuts against the inner wall of the filter membrane (9). The pump body (15) is connected to and communicates with the discharge pipe (14). The right side of the discharge pipe (14) is connected to and communicates with the first connector (16).

5. The self-cleaning anti-clogging cell disruption and filtration device of claim 1, wherein, It also includes a high-pressure nozzle (18), a water supply pipe (17), a valve (19), and a second connector (20). The high-pressure nozzle (18) is fixedly mounted on the right side of the housing (1). The right side of the high-pressure nozzle (18) is connected to and communicates with the water supply pipe (17). The right side of the water supply pipe (17) is connected to and communicates with the valve (19). The right side of the valve (19) is connected to and communicates with the second connector (20).

6. The self-cleaning, anti-clogging, cell disruption and filtration device of claim 1, wherein, It also includes a rotating shaft (21) and a cleaning brush (22), wherein the rotating shaft (21) is fixedly connected to the top of one of the drive wheels (7), and the cleaning brush (22) is fixedly connected to the rotating shaft (21), and the cleaning brush (22) abuts against the outer wall of the filter membrane (9).

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