Zebra fish injection device

Abstract

The utility model discloses a zebra fish injection device which comprises a culture dish box body and a unit plate, a support is arranged at the bottom of the unit plate and used for lifting the unit plate, a groove is formed in the unit plate, a drainage hole is formed in the side face of the groove, and the drainage hole and the bottom of the groove are arranged in a spaced mode. The utility model aims to solve the technical problems in the prior art, and provides the zebra fish injection device, which can transfer zebra fish to the injection vessel at one time and maintain the zebra fish in a proper injection water volume, thereby simplifying the complicated operation process.

Description

Technical Field

[0001] The utility model relates to the technical field of zebrafish embryo microinjection, in particular to a zebrafish injection device. Background Art

[0002] Zebrafish is a vertebrate model animal. Because of its similarity to human genes, short reproductive cycle, in vitro growth, and transparent embryos and larvae, it has been one of the important research objects of life scientists in recent decades. It has very important research value in biological genetics, development, toxicology, etc. Microinjection is the most commonly used technique in zebrafish research. It is used to inject specific substances such as nucleic acids, proteins or specific drugs into embryos or larvae.

[0003] At present, there are several types of injection dishes and their production molds on the market:

[0004] Patent CN203794894U discloses a special culture dish for zebrafish embryo microinjection. The upper end surface of the bottom of the culture dish box body is processed with at least two square grooves along the length direction. The number of culture unit plates is set in accordance with the number of square grooves. Each square groove is provided with a culture unit plate. The upper end surface of the culture unit plate is arranged with hemispherical embryo grooves in a matrix. The bottom of each hemispherical embryo groove is provided with a drainage hole, and each hemispherical embryo groove is provided with a number. The bottom of the culture dish is provided with a drainage hole, and all the water will leak out from the hole; zebrafish embryos are wrapped with egg membranes and can survive for a certain period of time without water, so they are suitable for zebrafish embryo injection. After the zebrafish fry is dehydrated, the state will deteriorate or even die, so the injection dish is not suitable for zebrafish fry injection.

[0005] Patent CN216001119U discloses a mold for making a zebrafish embryo injection dish, the mold comprising a main body and a molding part; wherein a plane is provided on the main body, the molding part is connected and arranged on the plane, and comprises a plurality of ridges arranged parallel and side by side along the extension direction of the plane; the extension direction of the ridge along the plane is defined as its length direction, and the direction in which the ridges are arranged side by side is defined as its width direction, and the ridges are provided with a plurality of notches that penetrate the width direction of the ridges at intervals along its length direction; when the mold is forming the injection dish, the molding part is immersed in liquid agarose: after the liquid agarose is solidified, a receiving slot for accommodating a zebrafish embryo is formed between two adjacent notches on the same ridge. The utility model designs the receiving slot as a triangular prism slot, so that the zebrafish embryo can be fixed after entering the slot and will not roll with the water flow, which is convenient for microinjection operation and counting, and is convenient for distinguishing injected and non-injected embryos.

[0006] Patent CN218227534U discloses a mold for making a zebrafish fry injection dish, which includes a main body and a molding part; a plane is provided on the main body, and the molding part is connected and arranged on the plane; the molding part includes a plurality of ridges arranged in parallel along the extension direction of the plane, and the extension direction of the ridges along the plane is defined as its length direction, and the direction in which the ridges are arranged side by side is its width direction, and the ridges are arranged in an M×N matrix: the ridges are arranged as semi-arc convex structures, and along the length direction, the two sides of the semi-arc convex structure are perpendicularly connected to the plane; along the width direction, one side of the semi-arc convex structure is perpendicularly connected to the plane, and the other side of the semi-arc convex structure is connected to the plane in an arc shape.

[0007] The above two molds can keep the zebrafish relatively fixed after being placed in the groove and will not move. However, in actual operation, we found that the process of transferring the zebrafish to the groove is quite cumbersome. Using a straw to suck up zebrafish embryos or larvae and accurately place them in the groove cannot be completed in one go, and multiple repeated operations are required to achieve the transfer. In addition, after the transfer, in order to ensure the stability of the zebrafish and the smooth progress of the injection, we also need to frequently adjust the amount of water in the groove. Too much water will cause the fish to float and affect the injection operation; too little water may worsen the condition of the fish or even cause death. This process of repeatedly adjusting the water volume is not only time-consuming, but also very easy to cause damage to the fish. Utility Model Content

[0008] 1. Technical problems to be solved by the utility model

[0009] The utility model aims to solve the technical problems existing in the prior art and provides a zebrafish injection device, which can transfer the zebrafish to an injection dish at one time and maintain it in a suitable amount of water for injection, thereby simplifying the complicated operation process.

[0010] 2. Technical solution

[0011] In order to solve the above problems, the technical solution provided by the utility model is:

[0012] A zebrafish injection device comprises a culture dish box and a unit plate, wherein a bracket is provided at the bottom of the unit plate for lifting the unit plate, a groove is provided on the unit plate, a drainage hole is provided on the side of the groove, and the drainage hole is set at a distance from the bottom of the groove.

[0013] Optionally, the groove is a V-shaped groove, including a vertical surface and a slope surface, and the drainage hole is arranged on the slope surface.

[0014] Optionally, the included angle between the vertical surface and the slope surface is 30°.

[0015] Optionally, the unit plate is an agar plate.

[0016] Optionally, the thickness of the unit plate is 4-7 mm.

[0017] Optionally, the diameter of the drainage hole is 2 mm.

[0018] Optionally, the height of the drainage hole from the bottom of the groove is 2 mm.

[0019] Optionally, the bracket is detachably connected to the unit plate.

[0020] 3. Beneficial effects

[0021] Compared with the prior art, the technical solution provided by the utility model has the following beneficial effects:

[0022] The zebrafish injection device can transfer the zebrafish to the injection dish at one time and maintain it in a water volume suitable for injection, which simplifies the complicated operation process; the unit plate can be extracted at will, which is convenient for cleaning and replacement, and can greatly improve the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the structure of a zebrafish injection device proposed in an embodiment of the utility model;

[0024] Figure 2 A cross-sectional schematic diagram of a zebrafish injection device proposed in an embodiment of the utility model;

[0025] 1. Culture dish box body; 2. Unit plate; 3. Bracket; 4. Groove; 4a. Vertical surface; 4b. Sloped surface; 5. Drainage hole. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods. It should be understood that the specific implementation methods described here are only used to explain the utility model and do not limit the protection scope of the utility model.

[0027] It should be noted that when an element is referred to as being "fixed to", "disposed on", "fixed on" or "installed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. Further, when an element is considered to be "fixedly connected to" another element, the two may be fixed in a detachable connection manner or in a non-detachable connection manner, such as socketing, snap-on, one-piece fixation, welding, etc., which can be achieved in the prior art and will not be repeated here. When an element is perpendicular or approximately perpendicular to another element, it means that the ideal state of the two is vertical, but due to the influence of manufacturing and assembly, there may be a certain vertical error. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0029] The “first” and “second” involved in the present invention do not represent specific quantities and orders, but are merely used to distinguish names.

[0030] Combined with Figure 1-2, a zebrafish injection device of the present embodiment comprises a culture dish box body 1 and a unit plate 2, the culture dish box body 1 is a circular box body with an open top, the unit plate 2 is placed in the culture dish box body 1, a bracket 3 is provided at the bottom of the unit plate 2 for lifting the unit plate 2, the bracket 3 is provided on both sides of the culture dish box body 1, specifically a plastic bracket, the unit plate 2 can be easily mounted on the bracket 3, a long groove 4 is provided on the unit plate 2 along its length direction, the left and right ends of the groove are closed, the upper end of the groove 4 is open, and a drainage hole 5 is provided on the side of the groove 4, and the drainage hole 5 is set at a distance from the bottom of the groove 4. In this embodiment, the groove 4 can accommodate a maximum of 2.8mL of water. When performing an injection experiment, the operator can use a straw to suck 3mL of water and zebrafish fry at one time, and slowly put them into the groove 4 of the unit plate 2. The drainage hole design of the groove 4 ensures that when water is injected, the excess water will be discharged through the drainage hole 5 until the remaining water in the groove 4 is about 300-500μL. This amount of water ensures that the zebrafish fry can be completely immersed in it, and the fish will not float up, nor will they die due to insufficient water. After the injection experiment is completed, the operator can easily pull out the unit plate 2 from the bracket 3 and perform necessary cleaning. At the same time, the residual water at the bottom of the unit plate 2 can also be directly poured out.

[0031] As a preferred embodiment of the present invention, the groove 4 is a V-shaped groove, including a vertical surface 4a and a slope surface 4b. The included angle between the vertical surface 4a and the slope surface 4b is 30°. If the angle is too large, the injection space will be narrow, which is inconvenient for injection; if the angle is too small, the zebrafish will be difficult to fix. The drainage hole 5 is provided on the slope surface 4b. After the zebrafish is placed in the groove 4, the vertical surface 4a can be abutted and relatively fixed in the groove 4, which is convenient for subsequent injection.

[0032] As a preferred embodiment of the utility model, the unit plate 2 is an agar plate. The surface of the agar plate is soft and smooth, which will not damage the zebrafish, and the needle is not easy to break when it touches the plate surface. Because when performing the injection, we still need to use the injection needle made of capillary tube and move it to the appropriate position. Due to the compact design of the card slot, the injection needle is very easy to touch the card slot wall during the moving process, resulting in the needle breaking, so that the injection cannot be performed.

[0033] As a preferred solution of the utility model, the thickness of the unit plate 2 is 4-7 mm. Too thin is not conducive to the production of the groove 4, and too thick affects drainage. This thickness makes the groove easy to produce and water can be discharged smoothly.

[0034] As a preferred solution of the utility model, the diameter of the drain hole 5 is 2 mm. The height of the drain hole 5 from the bottom of the groove 4 is 2 mm. This design can ensure that the zebrafish will not leak out of the drain hole 5, and the drain hole 5 will not be completely blocked by the body of the zebrafish so that water cannot be discharged.

[0035] As a preferred solution of the present invention, the bracket 3 is detachably connected to the unit plate 2, and the bracket 3 can be extracted or replaced at any time according to experimental needs.

[0036] The above schematically describes the present invention and its implementation methods, which are not restrictive. The drawings show only one implementation method of the present invention, and the actual structure is not limited thereto. Therefore, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A zebrafish injection device, characterized in that: The invention comprises a culture dish box body and a unit plate, wherein a bracket is arranged at the bottom of the unit plate for lifting the unit plate, a groove is arranged on the unit plate, a drainage hole is arranged on the side of the groove, and the drainage hole is set at a distance from the bottom of the groove.

2. A zebrafish injection device according to claim 1, characterized in that: The groove is a V-shaped groove, including a vertical surface and a slope surface, and the drainage hole is arranged on the slope surface.

3. A zebrafish injection device according to claim 2, characterized in that: The included angle between the vertical surface and the slope surface is 30°.

4. A zebrafish injection device according to any one of claims 1 to 3, characterized in that: The unit plate is an agar plate.

5. A zebrafish injection device according to claim 4, characterized in that: The thickness of the unit plate is 4-7 mm.

6. A zebrafish injection device according to claim 4, characterized in that: The diameter of the drainage hole is 2 mm.

7. A zebrafish injection device according to claim 4, characterized in that: The height of the drainage hole from the bottom of the groove is 2 mm.

8. A zebrafish injection device according to any one of claims 1 to 3, characterized in that: The bracket is detachably connected to the unit plate.

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