Fluid pool for clamping solid nanopore chip

Abstract

The utility model discloses a fluid pool for clamping a solid nanopore chip, belongs to the technical field of fluid pools, and aims to solve the problems that an existing device is complex in design and high in cost, the chip is easily damaged due to misoperation, the rapid liquid injection efficiency is low, a product is damaged due to repeated disassembly, and the service life and the detection effect are influenced. The sample adding device comprises an integrated sample adding pool, sample adding holes are symmetrically formed right above the two sides of the integrated sample adding pool, a push-pull type clamping groove is formed in the middle of the interior of the integrated sample adding pool, a groove is formed in the middle of the clamping groove, the nanopore chip is placed in the groove, a solution flows through a solution hole in the middle, and the nanopore chip is placed in the solution hole in the middle of the integrated sample adding pool. The left side and the right side of the integrated sample adding pool are respectively provided with a silver wire electrode interface located right above the integrated sample adding pool, and the silver wire electrode interfaces are located at the inner sides of the sample adding holes.

Description

TECHNICAL FIELD

[0001] The utility model relates to a kind of fluid pools, more specifically to a kind of fluid pool of clamping solid nanopore chip. BACKGROUND

[0002] Since the first solid nanopore chip was manufactured in 2001, solid nanopore detection has experienced a vigorous development. With the development of science and technology for decades, it has been proven that solid nanopore technology has unique advantages in virus, bacteria, protein detection and sequencing. However, as a necessary detection consumable for solid nanopore detection, the fluid pool is still in the stage of full manual installation and detection.

[0003] To solve the above problems, a fluid pool device for clamping a solid nanopore chip is disclosed in Chinese Patent No. CN118109268A. The device includes a housing assembly, a liquid pool assembly, a nanopore chip, a locking mechanism, and a sealing element. The liquid pool assembly includes a left liquid pool and a right liquid pool, which are installed in the receiving cavity of the housing body. The head of the sealing element is fixedly clamped in the limiting groove group I or the limiting groove group II, and the waist-shaped hole on the sealing element is fitted on the outer wall of the first boss or the third boss. The nanopore chip is fixedly clamped between the two sealing elements. The locking mechanism is provided in the blind hole of the left liquid pool. When the left and right liquid pools are installed in the installation cavity, the locking mechanism is in contact with the inner wall of the receiving cavity, which presses the spring inward and seals and clamps the left and right liquid pools. The device can effectively ensure the clamping and fixing of the nanopore chip by the sealing element, and improve the sealing performance under the condition of ensuring the smoothness of the flow channel.

[0004] However, the above device has a complex structure design. If not handled properly or the clamping force of the lock tongue and spring is too large, it may cause damage to the chip, especially when the chip is thin or fragile. Moreover, the excessive number of lock tongues, springs, limiting grooves, and sealing elements increases the manufacturing difficulty and cost, especially for large-scale production and disposable use scenarios, where cost may become a limiting factor. Since the solid nanopore detection device needs to be hydrophilized and pretreated (usually using piranha solution) before each use, repeated disassembly and assembly of the device may cause wear and damage to the product, affecting the service life of the fluid pool, and improper handling may also damage the chip. Although the tapered structure and inclined flow channel design of the liquid injection groove reduces the possibility of air bubble generation, in the case of rapid liquid injection or high-throughput application, the design of the flow channel may limit the flow rate of the liquid, resulting in low efficiency of liquid injection and drainage. SUMMARY

[0005] In view of the deficiencies of the prior art, the utility model provides a kind of fluid cell of clamping solid nanopore chip, including integrated sample adding pool, the left and right sides of the integrated sample adding pool, respectively the silver wire electrode interface of being located its directly above, integrated sample adding pool inside middle position is provided with push-pull type clamping groove, solution hole is equipped on the push-pull type clamping groove, the upper symmetry of the two sides of integrated sample adding pool is provided with sample adding hole, and the silver wire electrode interface is located the inboard of sample adding hole, recess is opened in the push-pull type clamping groove, and nanopore chip is arranged in the recess.

[0006] As a further improvement of the utility model, the two ends of the push-pull type clamping groove are arranged on the inner end face of the integrated sample adding pool by buckles.

[0007] As a further improvement of the utility model, one side of the silicon pad is provided with a second buckle, and the nanopore chip is fixedly connected to the silicon pad by the second buckle.

[0008] As a further improvement of the utility model, a sealing element is arranged between the push-pull type clamping groove and the inner end face of the integrated sample adding pool.

[0009] The fluid cell for clamping the solid nanopore chip provided by the device ensures that the nanopore is correctly installed in the fluid cell, ensures the clamping and fixing of the nanopore chip by the fixing buckle, avoids liquid leakage and ensures the accuracy and stability of detection, and specifically comprises:

[0010] 1. The left and right liquid pools are cancelled, and an integrated sample adding pool is designed. Compared with the existing fluid cell, this design improves the detection efficiency, simplifies the installation process, and reduces the wear between device elements and the damage of the chip or device caused by uneven stress during installation.

[0011] 2. The material of the sample adding pool is mainly plastic (fully transparent). This design facilitates the observation of the sample adding condition, thereby improving the detection accuracy and effectively reducing the cost.

[0012] 3. A pullable clamping groove is designed at the central position of the fluid cell. This design reduces the disassembly frequency of the device, making it more convenient to clean the solid nanopore chip before use and replace the damaged chip. The chip is fixed in the recess in the middle of the clamping groove, which reduces the pressure on the chip and reduces the risk of chip damage.

[0013] 4. When the pullable clamping groove is inserted into the sample adding pool, the solution can flow in the small hole of the clamping groove, thereby further improving the detection efficiency. The bottom of the clamping groove is provided with a first fixing buckle to ensure that the clamping groove can be stably fixed after being inserted into the sample adding pool. In addition, sealing elements are arranged on both sides of the clamping groove, which can maintain good sealing after being inserted into the sample adding pool, thereby avoiding the problem of liquid leakage.

[0014] 5. Silver wire electrode interface is designed symmetrically on the top of the left and right sides of the whole integrated sample adding pool, and silica gel washer is arranged on the insertion port. The electrode can be vertically inserted from the interface, and the electrode can be in good contact with the electrode joint and is not easy to slip off due to the gravity of the electrode wire itself and the effect of the silica gel washer. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 is the structure top view of the utility model;

[0016] Fig. 2 is the recess part enlarged schematic view of the utility model;

[0017] Mark: 1, integrated sample adding pool, 2, silver wire electrode interface, 3, push-pull type clamping groove, 4, sample adding hole, 5, recess, 6, nanometer hole chip, 7, first buckle, 8, silica pad, 9, second buckle, 10, movable slot. DETAILED DESCRIPTION

[0018] In order to facilitate understanding of the present application, the present application will be more fully described below with reference to the relevant drawings. The embodiments described in the drawings. Conversely, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0019] As Figs. 1-2 shown, a fluid pool for clamping a solid nanometer hole chip comprises an integrated sample adding pool 1, silver wire electrode interfaces 2 are arranged on the top of the left and right sides of the integrated sample adding pool 1 respectively, a push-pull type clamping groove 3 is arranged at the middle position inside the integrated sample adding pool 1, a solution hole is arranged on the push-pull type clamping groove 3, sample adding holes 4 are symmetrically arranged on the top of the two sides of the integrated sample adding pool 1, and the silver wire electrode interfaces 2 are located on the inner side of the sample adding holes 4, recesses 5 are arranged on the push-pull type clamping groove 3, and nanometer hole chips 6 and silica pads 8 are placed in the recesses 5. Movable slots 10 are arranged on the inner end surface of the recesses 5, silica pads 8 are arranged on the inner end surface of the movable slots 10, nanometer hole chips 6 are clamped on the silica pads 8, the movable slots 10 can be pulled out, and the left two second buckles 9 are used to fix the silica pads 8 and the nanometer hole chips 6. The two ends of the push-pull type clamping groove 3 are arranged on the inner end surface of the integrated sample adding pool 1 through first buckles 7. The outer side of the nanometer hole chip 6 is tightly combined with the inner side of the silica pad 8. The nanometer hole chip 6 is tightly fixed with the silica pad 8 through the second buckle 9. Sealing elements are arranged between the push-pull type clamping groove 3 and the inner end surface of the integrated sample adding pool 1.

[0020] A fluid pool for clamping a solid nanometer hole chip, the shell of which is an integrated sample adding pool 1, is used to carry sample liquid and buffer solution. The nanometer hole chip 6 is fixed in the recess 5 of the push-pull type clamping groove 3, and the chip is processed when manufactured and can be used at any time after cleaning treatment; the solution hole on the push-pull type clamping groove 3 is a solution flow channel.

[0021] The push-pull type clamping groove 3 is composed of two components, a groove is formed in the middle, and is designed for placing the nanopore chip 6. When the push-pull type clamping groove 3 is inserted into the integrated sample adding pool 1, it divides the integrated sample adding pool 1 into two areas. In addition, a sealing element is arranged between the push-pull type clamping groove 3 and the inner end face of the integrated sample adding pool 1 to ensure the sealing performance of the whole system and prevent liquid leakage, wherein the sealing element is made of rubber or silicone. The material of the integrated sample adding pool 1 is insulating and transparent.

[0022] The left and right upper parts of the integrated sample adding pool 1 are respectively provided with silver wire electrode interfaces 2 for connecting external power supply or detection equipment. The interfaces are provided with sealing rubber rings to prevent liquid leakage and silver wire electrode head slipping. A sample adding hole 4 is arranged beside the silver wire electrode interface 2, which is convenient for experimenters to inject sample liquid and buffer into the integrated sample adding pool 1.

[0023] The material of the sealing element is rubber or silicone, and the material of the integrated sample adding pool is insulating and transparent.

[0024] The device adopts an integrated sample adding pool 1 design to improve detection efficiency, and adopts a push-pull type clamping groove structure for the nanopore chip 6 to reduce the disassembly frequency of the device, facilitate the cleaning and replacement of the chip. At the same time, the design avoids the problem of chip damage caused by excessive spring force or uneven installation stress. The push-pull type clamping groove 3 is provided with a fixed buckle and a sealing element at both ends to ensure its stability and sealing performance, prevent liquid leakage, and thus avoid the influence of interfering current on detection. In addition, the silver wire electrode interface 2 is changed to the left and right upper parts and is provided with a silicone gasket, which also ensures good contact between the electrode and the electrode interface and prevents the electrode from slipping off, thereby preventing adverse effects on detection. The device is easy to disassemble as a whole, has few parts, the integrated sample adding pool is made of insulating and transparent material, can be repeatedly washed and disassembled, reduces cost, and is suitable for large-scale production and use and disposable use.

[0025] The above-described embodiments only express several implementation manners of the present application, the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the application patent. It should be noted that for ordinary skilled persons in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.

Claims

1. A fluidic pool for mounting a solid-state nanoporous chip, characterized in that, The sample includes an integrated sample loading chamber (1). On the left and right sides of the integrated sample loading chamber (1), there are silver wire electrode interfaces (2) located directly above them. A push-pull type slot (3) is provided in the middle of the integrated sample loading chamber (1). The push-pull type slot (3) is provided with a solution hole. Sample loading holes (4) are symmetrically arranged on the upper sides of the integrated sample loading chamber (1). The silver wire electrode interface (2) is located inside the sample loading hole (4). A groove (5) is opened on the push-pull type slot (3). A movable groove (10) is provided on the inner end face of the groove (5). A silicon pad (8) is provided on the inner end face of the movable groove (10). A nanoporous chip (6) is attached to the silicon pad (8).

2. The fluidic pool for mounting a solid-state nanoporous chip according to claim 1, characterized in that, The two ends of the push-pull slot (3) are set on the inner end face of the integrated sample filling pool (1) by the first buckle (7).

3. The fluidic pool for mounting solid-state nanoporous chips according to claim 2, characterized in that, A second snap fastener (9) is installed on one side of the silicon pad (8), and the nanopore chip (6) is tightly fixed to the silicon pad (8) by the second snap fastener (9).

4. The fluidic pool for mounting a solid-state nanoporous chip according to claim 3, characterized in that, A sealing element is provided between the push-pull slot (3) and the inner end face of the integrated sample filling pool (1).

Images