Multifunctional cryotubes of Artemisia callus suspension cells

Abstract

This invention discloses a multifunctional cryotube for Artemisia callus suspension cells, comprising a tube body and a cap. The cap is located at the top of the tube body, and a first connecting component is located on the opposite side of the tube body and cap, allowing the cap to be detachably connected to the tube body via the first connecting component. A sealing component is located on the side of the cap facing the tube body, improving the seal when the cap is connected to the tube body. A positioning component is located at the bottom of the tube body, and a second connecting component is located on the opposite side of the positioning component and tube body, allowing the positioning component to be detachably connected to the tube body via the second connecting component, and used for positioning the cryotube. A vibration assist component is located on the inner surface of the tube body's sidewall, enhancing the disturbance to the fluid within the tube during vibration. The advantages of this invention are: excellent sealing effect, good fixation on different tube racks and processing equipment, and rapid vibration of the Artemisia callus suspension cell fluid within the tube.

Description

Technical Field

[0001] This utility model relates to the field of cryotube technology, and in particular to a multifunctional cryotube for suspension cells of Artemisia callus. Background Technology

[0002] Artemisia argyi callus suspension cells refer to callus tissue induced by Artemisia argyi explants and dispersed in liquid culture medium through in vitro culture technology, forming free cells or small cell clusters that can proliferate stably.

[0003] When transporting and processing Artemisia callus suspension cells, cryotubes are needed to hold them to facilitate subsequent processing.

[0004] Existing refrigeration tubes have the following disadvantages when in use:

[0005] 1. Poor sealing effect; during handling and processing, the suspended cells of the mugwort callus tissue are easily spilled.

[0006] 2. Unable to achieve proper positioning on different pipe racks and processing equipment;

[0007] 3. During oscillation, the fluid inside the refrigeration tube does not oscillate well and requires a longer oscillation time.

[0008] This invention addresses these issues by proposing a multifunctional cryotube for Artemisia callus suspension cells, aiming to solve the aforementioned problems. The cryotube boasts excellent sealing performance, can be well fixed on different tube racks and processing equipment, and allows for rapid agitation of the Artemisia callus suspension cell fluid within the tube. Utility Model Content

[0009] To address the aforementioned shortcomings in the existing technology, this utility model provides a multifunctional cryotube for Artemisia callus suspension cells.

[0010] To achieve the aforementioned objectives, the technical solution adopted by this utility model is as follows:

[0011] Multifunctional cryotubes for Artemisia callus suspension cells, including tube body and cap.

[0012] A pipe cover is provided on the top of the pipe body, and a first connecting component is provided on the opposite side of the pipe body and the pipe cover. The pipe cover is detachably connected to the pipe body through the first connecting component.

[0013] The pipe cap is provided with a sealing component on the side facing the pipe body, and the sealing component improves the sealing performance when the pipe cap is connected to the pipe body;

[0014] A positioning component is provided below the tube body, and a second connecting component is provided on the side of the positioning component opposite to the tube body. The positioning component is detachably connected to the tube body through the second connecting component. The positioning component is used for the placement and positioning of the freezing tube.

[0015] The inner surface of the side wall of the pipe is provided with an oscillation auxiliary component, which enhances the disturbance to the fluid inside the pipe during oscillation.

[0016] As an improvement, the first connecting component includes a connecting edge and a connecting ring. The connecting edge is disposed on the upper end face of the tube body, and the outer surface of the connecting edge is provided with an external thread. The connecting ring is disposed on the lower end face of the tube cap, and the inner surface of the connecting ring is provided with an internal thread. The connecting ring is threadedly connected to the connecting edge.

[0017] As an improvement, the sealing component includes a sealing ring and a soft plug. The sealing ring is provided on the outer edge of the lower end face of the tube cap. The upper end of the outer surface of the tube is slidably connected to the sealing ring. The soft plug is provided at the center of the lower end face of the tube cap. The soft plug is slidably connected to the inner surface of the upper end of the tube.

[0018] As an improvement, a sealing ring one is provided on the inner surface of the lower end of the sealing ring, and a sealing ring two is provided on the outer surface of the lower end of the soft plug.

[0019] As an improvement, the outer surface of the sealing ring is uniformly provided with anti-slip stripes, and the extension direction of the anti-slip stripes is consistent with the height direction of the pipe cap.

[0020] As an improvement, the positioning component includes a base block and a insertion groove. The base block is connected to the tube body through a second connecting component, and the insertion groove is provided at the center of the lower end face of the base block.

[0021] As an improvement, the second connecting component includes a connecting block and a connecting groove. The connecting block is provided on the lower end face of the tube body, and the outer surface of the connecting block is provided with an external thread. The connecting groove is provided on the upper end face of the bottom block, and the inner surface of the connecting groove is provided with an internal thread. The connecting block is threadedly connected to the connecting groove.

[0022] As an improvement, the oscillation auxiliary component adopts vertical plates, which are evenly distributed on the lower end of the inner surface of the tube, and the included angle between two adjacent vertical plates is 120°.

[0023] As an improvement, a scale strip is provided on the outer surface of the tube.

[0024] Compared to traditional technologies, the advantages of this utility model are:

[0025] 1. When the cap and body are connected, the sealing component improves the sealing performance when the cap and body are connected, preventing the suspension of cell fluid from the mugwort callus tissue inside the tube from spilling out.

[0026] 2. The positioning component is detachably connected to the tube body through the second connecting component. The positioning component with different shaped insertion slots can be replaced according to the shape of the tube rack and equipment insertion component, so that the refrigeration tube can be well fixed on different tube racks and processing equipment.

[0027] 3. An oscillation auxiliary component is provided at the lower end of the pipe body. The oscillation auxiliary component enhances the disturbance to the fluid in the pipe body during oscillation, thereby improving the oscillation effect and efficiency. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the structure of the present invention. Figure 1 ;

[0029] Figure 2 This is a schematic diagram of the structure of the present invention. Figure 2 ;

[0030] Figure 3 This is a cross-sectional view of the pipe cap of this utility model;

[0031] Figure 4 This is a schematic diagram of the tube body of this utility model;

[0032] Reference table for attached figures:

[0033] 1. Pipe body; 2. Pipe cap; 3. Connecting edge; 4. Connecting ring; 5. Sealing ring; 6. Soft plug; 7. Sealing ring one; 8. Sealing ring two; 9. Anti-slip stripes; 10. Base block; 11. Insertion groove; 12. Connecting block; 13. Connecting groove; 14. Vertical plate; 15. Scale strip. Detailed Implementation

[0034] The specific embodiments of this utility model will be further described below with reference to the accompanying drawings. Identical components are indicated by the same reference numerals.

[0035] It should be noted that the terms “front,” “back,” “left,” “right,” “up,” and “down” used in the following description refer to the directions shown in the attached diagram, while the terms “inside” and “outside” refer to the directions toward or away from the geometric center of a specific component, respectively.

[0036] To make the content of this utility model easier to understand, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of this utility model.

[0037] Example 1

[0038] Combined with appendix Figure 1-3 The pipe body 1 is provided with a pipe cover 2 above it. The pipe body 1 and the pipe cover 2 are provided with a first connecting component on the opposite side. The pipe cover 2 is detachably connected to the pipe body 1 through the first connecting component.

[0039] The pipe cap 2 is provided with a sealing component on the side facing the pipe body 1, and the sealing component improves the sealing performance when the pipe cap 2 is connected to the pipe body 1.

[0040] The first connecting component includes a connecting edge 3 and a connecting ring 4. The connecting edge 3 is disposed on the upper end face of the tube body 1, and the outer surface of the connecting edge 3 is provided with an external thread. The connecting ring 4 is disposed on the lower end face of the tube cover 2, and the inner surface of the connecting ring 4 is provided with an internal thread. The connecting ring 4 is threadedly connected to the connecting edge 3.

[0041] The sealing component includes a sealing ring 5 and a soft plug 6. The sealing ring 5 is provided on the outer edge of the lower end face of the tube cap 2. The upper end of the outer surface of the tube body 1 is slidably connected to the sealing ring 5. The soft plug 6 is provided at the center of the lower end face of the tube cap 2. The soft plug 6 is slidably connected to the inner surface of the upper end of the tube body 1.

[0042] In use, pour the Artemisia callus suspension into tube 1, and connect tube cap 2 to the upper end of tube 1 via connecting ring 4 and threaded connection 3. When tube cap 2 is connected to tube 1, sealing ring 5 is fitted on the outside of tube 1, and soft plug 6 is inserted into the upper end of tube 1. The sealing ring 5 and soft plug 6 work together to effectively improve the sealing effect when tube cap 2 and tube 1 are connected.

[0043] Combined with appendix Figure 1-2 A positioning component is provided below the tube body 1, and a second connecting component is provided on the side of the positioning component opposite to the tube body 1. The positioning component is detachably connected to the tube body 1 through the second connecting component. The positioning component is used for the placement and positioning of the freezing tube.

[0044] The positioning component includes a base block 10 and a insertion groove 11. The base block 10 is connected to the tube body 1 through a second connecting component. The insertion groove 11 is provided at the center of the lower end face of the base block 10.

[0045] The second connecting component includes a connecting block 12 and a connecting groove 13. The lower end face of the tube body 1 is provided with a connecting block 12, the outer surface of the connecting block 12 is provided with an external thread, the connecting groove 13 is provided on the upper end face of the bottom block 10, the inner surface of the connecting groove 13 is provided with an internal thread, and the connecting block 12 is threadedly connected to the connecting groove 13.

[0046] When it is necessary to place cryotubes or process Artemisia callus suspension cell fluid, the positioning components of the insertion slots 11 of different shapes are replaced according to the shape of the insertion blocks on the tube rack or processing equipment. The bottom block 10 with the insertion slots 11 is threadedly connected to the connecting block 12 at the lower end of the tube body 1 through the connecting groove 13. The bottom block 10 can be flexibly and quickly disassembled and assembled with the tube body 1, so that the cryotube can be well fixed on different tube racks and processing equipment.

[0047] Combined with appendix Figure 4The inner surface of the side wall of the pipe body 1 is provided with an oscillation auxiliary component, which enhances the disturbance to the fluid inside the pipe body 1 during oscillation;

[0048] The vibration auxiliary component adopts a vertical plate 14, which is evenly distributed on the lower end of the inner surface of the tube body 1, and the included angle between two adjacent vertical plates 14 is 120°.

[0049] During the oscillation, the vertical plate 14 at the lower end of the inner surface of the side wall of tube 1 enhances the disturbance of the suspended cell fluid of the Artemisia callus tissue inside tube 1, thereby improving the effect and efficiency of oscillation.

[0050] Example 2

[0051] Combined with appendix Figure 1-3 The sealing ring 5 has a sealing ring 7 on its lower inner surface and a sealing ring 8 on its lower outer surface; the sealing ring 5 has anti-slip stripes 9 evenly distributed on its outer surface, and the extension direction of the anti-slip stripes 9 is consistent with the height direction of the tube cap 2; the tube body 1 has a scale strip 15 on its outer surface.

[0052] Based on Example 1, sealing ring 7 and sealing ring 8 further enhance the sealing effect when the tube cap 2 and tube body 1 are connected; the anti-slip stripes 9 on the outer surface of the sealing ring 5 facilitate the rotation of the tube cap 2; the scale strips 15 on the outer surface of the tube body 1 allow for direct viewing of the liquid level of the suspended cell fluid of the Artemisia callus tissue inside the tube body 1.

[0053] The above description is only a preferred embodiment of this utility model patent and is not intended to limit this utility model patent. Any modifications, equivalent substitutions and improvements made within the spirit and principles of this utility model patent should be included within the protection scope of this utility model patent.

Claims

1. A multifunctional cryotube for Artemisia callus suspension cells, comprising a tube body (1) and a tube cap (2), characterized in that: The tube body (1) is provided with a tube cap (2) on its upper part. A first connecting component is provided on the opposite side of the tube body (1) and the tube cap (2). The tube cap (2) is detachably connected to the tube body (1) through the first connecting component. The pipe cap (2) is provided with a sealing component on the side facing the pipe body (1), and the sealing component improves the sealing performance when the pipe cap (2) is connected to the pipe body (1); A positioning component is provided below the tube body (1), and a second connecting component is provided on the side of the positioning component opposite to the tube body (1). The positioning component is detachably connected to the tube body (1) through the second connecting component. The positioning component is used for positioning the freezing tube. The inner surface of the side wall of the pipe (1) is provided with an oscillation auxiliary component, which enhances the disturbance to the fluid inside the pipe (1) during oscillation.

2. The multifunctional cryotube for Artemisia callus suspension cells according to claim 1, characterized in that: The first connecting component includes a connecting edge (3) and a connecting ring (4). The connecting edge (3) is disposed on the upper end face of the tube body (1). The outer surface of the connecting edge (3) is provided with an external thread. The connecting ring (4) is disposed on the lower end face of the tube cap (2). The inner surface of the connecting ring (4) is provided with an internal thread. The connecting ring (4) is threadedly connected to the connecting edge (3).

3. The multifunctional cryotube for Artemisia callus suspension cells according to claim 1, characterized in that: The sealing component includes a sealing ring (5) and a soft plug (6). The sealing ring (5) is provided on the outer edge of the lower end face of the tube cap (2). The upper end of the outer surface of the tube body (1) is slidably connected to the sealing ring (5). The soft plug (6) is provided at the center of the lower end face of the tube cap (2). The soft plug (6) is slidably connected to the inner surface of the upper end of the tube body (1).

4. The multifunctional cryotube for Artemisia callus suspension cells according to claim 3, characterized in that: The sealing ring (5) has a sealing ring 1 (7) on the inner surface of its lower end, and the soft plug (6) has a sealing ring 2 (8) on the outer surface of its lower end.

5. The multifunctional cryotube for Artemisia callus suspension cells according to claim 4, characterized in that: The outer surface of the sealing ring (5) is uniformly provided with anti-slip stripes (9), and the extension direction of the anti-slip stripes (9) is consistent with the height direction of the pipe cap (2).

6. The multifunctional cryotube for Artemisia callus suspension cells according to claim 1, characterized in that: The positioning component includes a bottom block (10) and a insertion groove (11). The bottom block (10) is connected to the tube body (1) through a second connecting component. The bottom block (10) has an insertion groove (11) at the center of its lower end face.

7. The multifunctional cryotube for Artemisia callus suspension cells according to claim 6, characterized in that: The second connecting component includes a connecting block (12) and a connecting groove (13). The lower end face of the tube body (1) is provided with a connecting block (12). The outer surface of the connecting block (12) is provided with an external thread. The connecting groove (13) is provided on the upper end face of the bottom block (10). The inner surface of the connecting groove (13) is provided with an internal thread. The connecting block (12) is threadedly connected to the connecting groove (13).

8. The multifunctional cryotube for Artemisia callus suspension cells according to claim 1, characterized in that: The vibration auxiliary component adopts a vertical plate (14), which is evenly distributed on the lower end of the inner surface of the tube (1), and the included angle between two adjacent vertical plates (14) is 120°.

9. The multifunctional cryotube for Artemisia callus suspension cells according to claim 1, characterized in that: The outer surface of the tube (1) is provided with a scale strip (15).

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