Liquid oral composition with cod collagen and elastin

Abstract

Liquid oral composition as a food, food supplement or dietary food for improving skin elasticity, skin structure and supporting joint function, comprising hydrolyzed collagen peptides obtained from cod (Gadus spp. especially Gadus morhua) and hydrolyzed elastin peptides, characterized in that it is available as a ready-to-drink or instant powder-soluble oral solution and achieves a synergistically enhanced effect compared to conventional collagen-elastin combination preparations due to the combined, complementary amino acid profile of the active ingredients.

Description

Technical field:

[0001] The invention relates to a liquid oral composition with a synergistic effect on skin elasticity, skin structure, and joint function. It contains hydrolyzed cod collagen and hydrolyzed elastin. Reason for the novelty:

[0002] The invention is novel because a combination of cod collagen and elastin in liquid formulation has not previously been described in scientific literature, patents, or commercial products.

[0003] Although a patent specification (CN 110847297A, Infinitus Ltd.) exists with marine sources (including cod, bonito), it does not include liquid application.

[0004] Commercial elastin products are mostly derived from bovine or snapper skin, sometimes contain less than 20 mg of elastin per more than 1.5 g of collagen, and are available in powder form. The use of bonito elastin or cod elastin with very low molecular weight (<1000 Da) is also not state of the art. The invention uniquely utilizes a highly bioavailable matrix in liquid solution.

[0005] By focusing on marine elastin sources (e.g., from bonito or cod), the previously common use of elastin from bovine ligaments is avoided – the latter was problematic due to BSE and other zoonotic risks. This combination is therefore novel and differs from the state of the art. Technological progress: a) Broad amino acid spectrum of cod collagen

[0006] Cod collagen consists predominantly of type 1 collagen (skin type) and has an exceptionally high content of glycine, proline, and hydroxyproline, the most important building blocks of skin collagen. It also contains particularly high amounts of a broad spectrum of essential amino acids—those amino acids that the body cannot synthesize itself. The concentration of the amino acids glycine, proline, and hydroxyproline, which together make up more than 50% of all amino acids, is higher in this marine collagen hydrolysate compared to other animal sources such as beef, chicken, pork, or other fish. Furthermore, unlike other sources, cod collagen also contains cysteine ​​and a particularly high amount of its precursor methionine, which are crucial for collagen fiber formation.

[0007] Furthermore, it contains a higher proportion of the essential amino acids histidine, phenylalanine, and threonine, which are crucial for collagen formation and skin regeneration. It also contains a significantly higher proportion of serine, which makes up almost 20% of connective tissue. b) Synergistic amino acid supplementation through elastin and bonito-elastin

[0008] Elastin hydrolysate contains elastin-specific amino acids (desmosine, isodesmosine, allysine, valine, alanine) that are almost entirely absent in collagen. Elastin hydrolysate also contains significantly more of these amino acids, especially desmosine / isodesmosine, than other elastin sources (see [reference]). Fig. .

[0009] Thus, the collagen-based structural tissue (strength) is complemented by elastic components (extensibility). This pairing is biologically beneficial, as both proteins work together in skin and connective tissue; see [reference]. Fig. .

[0010] The illustration clearly shows the respective strengths of the two protein sources: collagen hydrolysate from cod with a high proportion of a total of approximately 16 structure-giving amino acids such as glycine, proline and hydroxyproline - and bonito elastin with functional supplementation by 5 additional elastin-specific amino acids such as desmosine, isodesmosine, cystine and tryptophan.

[0011] The combination also offers advantages in the joint area: Collagen peptides can positively influence cartilage structure and bone metabolism. Elastin, on the other hand, is found in ligaments, tendons, and skin, giving tissue elasticity. Recent studies suggest that elastin peptides not only stimulate elastin production (e.g., through the dipeptide Pro-Gly found in elastin) but can also promote bone mineralization. The composition can thus contribute holistically to joint health: Collagen hydrolysate provides essential building blocks for cartilage and strengthens its structural function, while elastin hydrolysate supports the flexibility of connective tissue, ligaments, and skin.

[0012] The simultaneous administration of both proteins with a coordinated profile thus enables targeted support of: • Skin strength (collagen) • Skin elasticity (elastin) • Connective tissue structure • Musculoskeletal system (joints, ligaments, tendons) c) Liquid formulation with high bioavailability

[0013] Liquid orally administered nutrients are generally absorbed by the body faster and more efficiently than powdered or solid nutrients. This is due to several factors: 1. Solubility: Liquid nutrients are already in dissolved form, which facilitates absorption in the small intestine. Powders, for example in capsules, must first dissolve in the digestive tract. 2. Surface contact: Liquid nutrients generally have a larger contact area with the intestinal cells that absorb the nutrients. This improves absorption, as more nutrients can be absorbed simultaneously. 3. Digestion time: Because liquid nutrients are already dissolved, they partially bypass the mechanical and chemical breakdown process that takes place in the stomach. They pass through the stomach faster than solid or powdered foods and reach the small intestine more quickly, where the main absorption occurs.

[0014] Studies show that di- and tripeptide fragments from fish collagen and bonito elastin are measurable in plasma as early as 30-60 minutes after oral administration.

[0015] Bonito elastin with molecular weights <1000 Da is exceptionally water-soluble and exhibits better absorption compared to elastin from bovine ligaments or fish skin.

[0016] The invention demonstrates a surprising synergistic effect on skin and joints that goes beyond what conventional collagen-elastin combination products offer.

[0017] Finally, marine collagen and elastin products are free from animal pathogens (no BSE risk, no pork components) and are mostly accepted as halal and kosher.

[0018] In summary, the proposed invention presents a novel combination of active ingredients which, through broad amino acid coverage, improved elasticity and structural effects, and superior bioavailability, offers increased benefits for skin, connective tissue, and joints. Amino acid profile (aminogram): Fig. (Annex) shows a comparative aminogram (approximate composition in wt.% of amino acids) of the oral composition of this invention (“Combination 1”) and a conventional composition of another fish collagen and bovine elastin (“Combination 2”).

[0019] Combination 1 offers the following 4 advantages: 1. Broader and more complete amino acid spectrum: Synergy between structure (collagen) and elasticity (elastin)

[0020] Combination 1 covers both structure-giving (e.g. glycine, hydroxyproline) and elasticity-promoting amino acids (e.g. desmosine, isodesmosine, cystine) more completely. • The graph shows noticeably higher values ​​for: Desmosine and isodesmosine. Bonito is the only known marine elastin with a significant amount of desmosine / isodesmosine. ◯ Cystine and allysine. These are almost entirely absent in bovine elastin. Tryptophan. This is only significantly present in combination 1. • Combination 2 shows significant gaps here, especially regarding elastin markers 2. Higher content of skin-relevant amino acids

[0021] Combination 1 provides more glycine, proline and hydroxyproline, which are essential for collagen formation, skin firmness and skin regeneration. • These values ​​are significantly higher in cod than in tilapia. For example, the glycine content is over 20% in cod, but significantly lower in tilapia. • Cod offers the highest-performing collagen profile 3. Improved bioavailability • Bonito-elastin has a low molecular weight < 1000 Da, ideal for effective gastrointestinal absorption. • Bovine elastin is high molecular weight (approx. 60-70 kDa) and more difficult to digest. • Bonito peptides remain stable even under acidic conditions (e.g., at stomach pH) and are water-soluble. This allows for better absorption in the small intestine. 4. Fish-based elastin source without BSE risk and with better consumer acceptance • Beef is more problematic from a legal and ethical standpoint (BSE, animal welfare). • Marine elastin is free from known zoonotic risks, halal / kosher compatible and more environmentally friendly.

[0022] Combination 1 is therefore technically, functionally and regulatory superior to combination 2 and represents an improvement. Concluding remark:

[0023] This invention combines for the first time specifically cod collagen peptides with elastin peptides, specifically bonito or cod elastin, in a ready-to-drink or instant powder-soluble drinking solution for targeted support of skin and joints.

[0024] The formulated combination stands out from the state of the art due to its broader, synergistic amino acid spectrum and is technically superior, particularly in its support of connective tissue's regenerative capacity, strength, and elasticity. Areas of application include: • Nutricosmetic applications (anti-aging, skin tightening, moisture retention, wrinkle reduction) • Joint health (cartilage regeneration, mobility, ligament elasticity) List of sources 1. US Patent 10,869,948 B2 (December 22, 2020) - Composition of collagen peptide and elastin peptide. (Disclosing a combination of collagen and elastin peptides, among others from cod and bonito; varying ratios and not a liquid formulation) 2. Alpha Foods - Collagen + Elastin + Hyaluronic Acid Powder (Product Information). (Example of a commercially available collagen-elastin product in powder form, bovine collagen + fish elastin, shows synergistic effects for skin and joints) 3. Wang et al. (2024) - Fish Collagen Tripeptide and Elastin Peptide Supplement Enhances Skin Health (Study, Shanghai Ninth People's Hospital). (Clinical study: oral intake of fish collagen and elastin peptides improves skin hydration, elasticity and reduces wrinkles) 4. Chen et al. (2021) - Synergistic Effects of Fish Collagen Peptide and Bonito Elastin Peptide (Biomedical Science & Research, Vol. 17). (In vitro study: Combination of fish collagen and elastin peptides increases collagen I production in fibroblasts more than individual components; elastin peptide (from skipjack) contains pro-gly sequences, promotes elastin formation and bone mineralization) 5. Zheng et al. (2021) - Elastin Structure, Synthesis, and Relationship With Cardiovascular Disease (Front. Cell Dev. Biol. 9:596702). (Review article: Elastin consists of >75% glycine, valine, alanine, proline; ~33% glycine, ~11% proline; long-lasting, cross-linked protein for tissue elasticity) 6. Saito M et al. (2021) - Low-molecular-weight elastin peptides and skin elasticity. Int J MolSci, 22(4):1897. 7. Savenkova et al. (2021) - Chemical Characterization of Atlantic Cod Collagen Hydrolysate (Mar. Drugs 19(9):502). (Analytical analysis: Amino acid profile of cod collagen is typical for fish collagen: very rich in glycine (~35%) and significant in hydroxyproline; hydrolysate highly water-soluble, low molecular weight) 8. Infinitus (2019) - Chinese Patent Appl. 201910036842.5. (Background information: Combination of elastin + collagen in small doses can increase elastin, collagen and hyaluronic acid levels and inhibit skin inflammation; elastin from animal sources replaced by fish elastin due to BSE concerns) QUOTES INCLUDED IN THE DESCRIPTION

[0000] This list of documents cited by the applicant was automatically generated and is included solely for the reader's convenience. The list is not part of the German patent or utility model application. The DPMA accepts no liability for any errors or omissions. Cited patent literature

[0000] CN 110847297A

[0003] US 10,869,948 B2

[0024] CN 201910036842.5

[0024] Cited non-patent literature

[0000] Wang et al. (2024) - Fish Collagen Tripeptide and Elastin Peptide Supplement Enhances Skin Health (Study, Shanghai Ninth People's Hospital). (Clinical study: oral intake of fish collagen and elastin peptides improves skin hydration, elasticity and reduces wrinkles

[0024] Chen et al. (2021) - Synergistic Effects of Fish Collagen Peptide and Bonito Elastin Peptide (Biomedical Science & Research, Vol. 17

[0024] Zheng et al. (2021) - Elastin Structure, Synthesis, and Relationship With Cardiovascular Disease (Front. Cell Dev. Biol. 9:596702

[0024] Saito M et al. (2021) - Low-molecular-weight elastin peptides and skin elasticity. Int J MolSci, 22(4):1897

[0024] Savenkova et al. (2021) - Chemical Characterization ofAtlantic Cod Collagen Hydrolysate (Mar. Drugs 19(9):502

[0024]

Claims

[1] Liquid oral composition for use as a food, food supplement or dietary food to improve skin elasticity, skin structure and support joint function, comprising hydrolyzed collagen peptides obtained from cod (Gadus spp. especially Gadus morhua) and hydrolyzed elastin peptides, characterized by , that it is available as a ready-to-drink or instant powder solution and achieves a synergistically enhanced effect compared to conventional collagen-elastin combination products due to the combined, complementary amino acid profile of the active ingredients. [2] Oral composition according to claim 1, characterized by that the elastin is obtained exclusively from marine sources. [3] Oral composition according to claim 2, characterized by , that the elastin is obtained from skipjack (Katsuwonus pelamis) or cod (Gadus spp. especially Gadus morhua). [4] Oral composition according to any one of the preceding claims, characterized by that the molecular weights of the peptides are below 3000 Da (collagen) or below 1000 Da (elastin). [5] Oral composition according to any one of the preceding claims, characterized by that the cod collagen peptides and / or the elastin peptides exist in the form of tripeptides. Tripeptides are smaller, with a defined, very short sequence.

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