Coenzyme Q10-containing composition

Abstract

To provide a coenzyme Q10-containing composition that exhibits excellent antioxidant activity. [Solution] A composition comprising coenzyme Q10 and one or more antioxidants selected from the following: water chestnut plant extract, red wine extract, tea extract, and onion extract.

Description

TECHNICAL FIELD

[0001] The present invention relates to a coenzyme Q10-containing composition. Specifically, the present invention relates to a coenzyme Q10-containing composition that provides a synergistic antioxidant effect by combining coenzyme Q10 (Component A) with at least one antioxidant substance (Component B) selected from the group consisting of Trapa genus plant extracts, red wine extracts, tea extracts, and onion extracts.

BACKGROUND ART

[0002] Normally, in the bodies of humans and animals, a balance is maintained between the amount of active oxygen generated and the amount of active oxygen reduced by active oxygen-scavenging enzymes, and the amount of active oxygen in the body is kept constant. However, it has been pointed out that active oxygen in the body increases under the influence of living environments such as aging, stress, and unbalanced diets. The presence of unnecessary active oxygen in the body causes oxidative damage, so-called oxidative stress, which harms various tissues and organs in the body, and exerts adverse effects such as accelerated aging, increased risk of lifestyle-related diseases, and increased carcinogenic risk.

[0003] Specifically, for example, it has long been known that active oxygen in the body is involved in cardiovascular and cerebrovascular diseases such as arteriosclerosis, myocardial infarction, cerebral infarction, and cerebral hemorrhage, as well as inflammatory diseases such as rheumatism, collagen diseases, inflammatory bowel diseases, and autoinflammatory diseases. In recent years, it is said that active oxygen is also involved in the development of cancer, lifestyle-related diseases such as diabetes and hyperlipidemia, and even sleep disorders, urination disorders, and dementia.

[0004] For these reasons, in recent years, there has been growing interest in supplementing the body's antioxidant function using antioxidants that have the effect of scavenging reactive oxygen species. Certain antioxidants are incorporated into pharmaceutical preparations for the purpose of preventing and treating diseases related to reactive oxygen species in the body, such as arteriosclerosis, heart diseases such as cerebral infarction and myocardial infarction that result from arteriosclerosis, lifestyle-related diseases such as cancer and diabetes, and dementia associated with aging. For example, the antioxidant probucol is known to be used in the treatment of hyperlipidemia, ubicarenone in the treatment of congestive heart failure symptoms, and edaravone as a neuroprotective agent to improve neurological symptoms, daily living activities impairment, and functional impairment associated with the acute phase of cerebral infarction. Furthermore, antioxidants such as α-tocopherol and ascorbic acid are incorporated into various pharmaceutical preparations.

[0005] Coenzyme Q10, also known as ubicarenone or coenzyme Q10, is a type of coenzyme found in higher animals. Coenzyme Q10 is known not only for its biological activity as a coenzyme, but also for its role in energy production and antioxidant effects.

[0006] Regarding the antioxidant activity of coenzyme Q10, it is known that the combined use of reduced coenzyme Q10 and carotenoids synergistically enhances the scavenging effect of reactive oxygen species and / or free radicals (Patent Document 1).

[0007] Furthermore, compositions containing coenzyme Q10 and ampelopsin with enhanced antioxidant capacity are also known (Patent Document 2). [Prior art documents] [Patent Documents]

[0008] [Patent Document 1] Japanese Patent Publication No. 2013-079287 [Patent Document 2] Japanese Patent Publication No. 2018-188371 [Non-patent literature]

[0009] [Non-Patent Document 1] S. Takeshita et al., “Reducing effect of the long term intake of water chestnut (Trapa bispinosa Roxb.) pericap extract on glycative stress in the placebo-controlled double blinded clinical trial and in vitro inhibitory actions on low-density lipoprotein (LDL) glycation”, Glycative Stress Research,Vol.4,No.4,299-316, 31 December 2017 [Non-Patent Document 2] C. Tomiya et al., “Impact of Intake of Water Chestnut Pericarp-derived Polyphenols on Voiding-related Quality of Life in Healthy Middle-aged and Older People -A Rndomized, Placebo-controlled, Double-blind, Parallel-group Study-”, Jpn Pharmacol Ther,Vol.51,No.12,1835-1845(2023) [Non-Patent Document 3] Matsufuji et al., DPPH radical scavenging activity in a two-component mixed system of antioxidants, Journal of the Japanese Society for Food Science and Technology, Vol. 56, No. 3, 129-136 (2009). [Overview of the project] [Problems that the invention aims to solve]

[0010] The object of the present invention is to provide a coenzyme Q10-containing composition that exhibits superior antioxidant effects. [Means for solving the problem]

[0011] As a result of diligent research to solve the above problems, the present inventors have discovered that combining coenzyme Q10 with one or more antioxidants from among water chestnut plant extract, red wine extract, tea extract, and onion extract results in a synergistic promotion of antioxidant activity through cooperative action, thus completing the present invention. [Modes for carrying out the invention]

[0012] Embodiments of the present invention include the following aspects. [1] A composition comprising coenzyme Q10 and one or more antioxidants selected from the following: water chestnut plant extract, red wine extract, tea extract, and onion extract. [2] The composition according to [1], wherein the mass ratio of antioxidants to coenzyme Q10 is 0.05 to 5.0. [3] An antioxidant composition comprising the composition described in [1] or [2] above as an active ingredient. [4] An oral composition comprising the composition described in [1] or [2] above as an active ingredient.

[0013] (Coenzyme Q10) The coenzyme Q10 used in this invention can be obtained by known means, such as extraction from living organisms, synthesis, or fermentation, without any particular limitations. Furthermore, various commercially available products can also be used. Examples of commercially available products include Kaneka QH® and Kaneka Coenzyme Q10® from Kaneka Corporation, ShiroQ® and 40SP from PetroEuroAsia Co., Ltd., and AquaQ from Nissin Pharma Co., Ltd. 10 Examples include (registered trademark) P40-NF, etc.

[0014] There are no particular restrictions on the amount of coenzyme Q10 in the composition of the present invention, but preferably 1 The amount is approximately 80% by mass, more preferably 5-40% by mass.

[0015] (antioxidants) Examples of the antioxidant substance used in the present invention include Trapa genus plant extracts, red wine extracts, tea extracts, and onion extracts. Among these, Trapa genus plant extracts, particularly water caltrop pericarp extracts, are preferred.

[0016] The content of the antioxidant substance in the composition of the present invention is not particularly limited, but the mass ratio thereof relative to coenzyme Q10 is preferably 0.05 to 5.0, more preferably 0.1 to 2.0, even more preferably 0.5 to 1.5, and still even more preferably 0.5 to 1.0.

[0017] (Trapa Genus Plant Extract) The Trapa genus plant extract is an extract extracted from the pericarp of a plant belonging to the Trapa genus of the Trapaceae family, particularly the pericarp of Trapa bispinosa Roxb. The pericarp of Trapa bispinosa is dried and consumed as a health tea. The Trapa genus plant extract has anti-glycation activity and antioxidant activity, and it has been confirmed to improve skin elasticity, suppress postprandial blood glucose elevation, improve total cholesterol level, and improve QOL associated with urination (Non-Patent Documents 1 and 2).

[0018] The Trapa genus plant extract used in the present invention is not particularly limited, and various products are commercially available, so these commercially available products can also be used. Examples of commercially available products include Trapa extracts manufactured by Hayashikane Sangyo Co., Ltd. and Kotobuki Chemical Co., Ltd.

[0019] (Red Wine Extract) The red wine extract is an extract extracted from red wine. Trans-resveratrol contained in the red wine extract has been confirmed to have sirtuin gene activation, antioxidant activity, neuroprotective effects and the like.

[0020] The red wine extract used in the present invention is not particularly limited, and various products are commercially available, so these commercially available products can also be used. Examples of commercially available products include ANOX manufactured by Nippon Yakuhin Co., Ltd., and Red Wine Effect R manufactured by Medience Co., Ltd..

[0021] (tea extract) Tea extract is an extract obtained from green tea leaves. Tea extract has been shown to have antibacterial activity, antioxidant activity, and effects that reduce body fat.

[0022] There are no particular restrictions on the tea extract used in this invention, but various commercially available products can also be used. Examples of commercially available products include Sunphenon® manufactured by Taiyo Kagaku Co., Ltd. and green tea extract manufactured by Bioactives Japan Co., Ltd.

[0023] (Onion extract) Onion extract is an extract derived from onions. Onion extract has been shown to have antioxidant activity, anti-inflammatory effects, and improve cognitive function.

[0024] There are no particular restrictions on the onion extract used in this invention, but various products are commercially available and can be used. Examples of commercially available products include Quercea manufactured by Biocorp Japan Co., Ltd. and onion peel extract powder manufactured by Taiho Co., Ltd. [Effects of the invention]

[0025] The composition of the present invention can be incorporated into pharmaceuticals, health foods, health drinks, and other food and beverage products, as well as animal feed, for the prevention and alleviation of symptoms of diseases involving reactive oxygen species, such as arteriosclerosis, cerebral infarction, heart disease, cancer, diabetes, sleep disorders, and dementia. [Examples]

[0026] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to the following examples.

[0027] [Evaluation of the antioxidant capacity of a coenzyme Q10-containing composition (containing components A and B), and example of tablet manufacturing] (raw materials) The following were used. Coenzyme Q10 For antioxidant capacity evaluation (product name "Ubiquinol", manufactured by TRC) For tablets (product name "Aqua Q") 10 (Registered Trademark) P40-NF, manufactured by Nissin Pharma Co., Ltd.) • Extract from the Trapa genus (product name "Hishi Extract", manufactured by Hayashikane Sangyo Co., Ltd.) • Red wine extract (product name "ANOX-R5", manufactured by Nippon Yakuhin Co., Ltd.) • Tea extract (product name "Sunphenon (registered trademark) 80-OP", manufactured by Taiyo Kagaku Co., Ltd.) • Onion extract (product name "Quercea 20", manufactured by Biocorp Japan) • Cellulose (product name "Ceolus®", manufactured by Asahi Kasei) • Maltose (product name "Sanmalto (registered trademark)", manufactured by Nagase Vita) • Calcium stearate (product name "Calcium Stearate", manufactured by Taihei Chemical Industry Co., Ltd.)

[0028] (Evaluation method) The procedure was carried out in reference to the method described in Non-Patent Document 3, which has high measurement accuracy and has been applied to the evaluation of two-component antioxidant systems. Specifically, an ethanol solution of a predetermined concentration was prepared for each test substance, and then an ethanol solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was added to evaluate the scavenging activity of DPPH radicals.

[0029] DPPH radical scavenging activity was measured using an antioxidant capacity measurement kit (DPPH Antioxidant Assay, manufactured by Dojin Chemical Laboratories Co., Ltd.), and the DPPH radical scavenging rate was calculated using the following formula. DPPH radical scavenging rate I(%) = {(Ac-As) / Ac} × 100 In the formula, As represents the absorbance of the test substance sample solution, and Ac represents the absorbance of the control solution.

[0030] (Evaluation method when two components are mixed) The DPPH radical scavenging rate of each test substance listed in Tables 1 and 2 was measured individually. Subsequently, the DPPH radical scavenging rate of the mixed samples (liquid mixtures) listed in Tables 1 and 2 was measured (measured value IM). The predicted elimination rate of the mixed sample was calculated using the following formula, and the synergistic effect of mixing was evaluated by comparing the predicted and measured elimination rates of the mixed sample. The predicted elimination rate of the mixed sample of test substance A (component A) and test substance B (component B) was calculated using the following formula. Predicted value IE(%) = IA + IB In the formula, IA represents the "elimination rate of test substance A," and IB represents the "elimination rate of test substance B." We considered synergistic effects to be present if the IM / IE ratio was 1.1 or higher.

[0031] Coenzyme Q10 exists in both reduced and oxidized forms, but only the reduced form possesses antioxidant activity. Oxidized coenzyme Q10 is converted to reduced coenzyme Q10 in the body after ingestion. It exhibits antioxidant properties. In this experiment, since it would be impossible to evaluate the antioxidant properties of coenzyme Q10 if the oxidized form (ubiquinone) was used, the reduced form (trade name "Ubiquinol," manufactured by TRC) was used.

[0032] (Example of tablet manufacturing) The ingredients listed in Table 3 were mixed and compressed into tablets of approximately 300 mg each using a tablet press to produce tablets containing coenzyme Q10.

[0033] [Examples 1-11 and Comparative Examples 1-3] (result) Synergistic effect of a composition containing coenzyme Q10 and a plant extract of the Trapa genus. [Table 1]

[0034] • Composition comprising coenzyme Q10 and red wine extract, tea extract, or onion extract. Synergistic effect [Table 2]

[0035] • Formulation of Coenzyme Q10-containing tablets (manufacturing example) [Table 3]

[0036] The results in Tables 1 and 2 above indicate that compositions containing coenzyme Q10 and one or more antioxidants from among water chestnut plant extract, red wine extract, tea extract, and onion extract exhibit synergistic antioxidant effects.

[0037] Therefore, by incorporating the composition of the present invention into pharmaceuticals, health foods, health drinks, and other food and beverage products for the prevention and alleviation of diseases caused by the action of reactive oxygen species, which have been pointed out as being involved in various health problems, it is expected that enhanced effects will be exhibited based on its excellent antioxidant activity, and it is particularly highly usable in the fields of food and health foods.

Claims

1. A composition comprising coenzyme Q10 and one or more antioxidants selected from the following: water chestnut plant extract, red wine extract, tea extract, and onion extract.

2. The composition according to claim 1, wherein the mass ratio of antioxidants to coenzyme Q10 is 0.05 to 5.

0.

3. An antioxidant composition comprising the composition described in claim 1 or 2 as an active ingredient.

4. An oral composition comprising the composition described in claim 1 or 2 as an active ingredient.

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