Drug structural coating having super-hydrophobic property and preparation method therefor

Abstract

The present application belongs to the technical field of biomedical functional materials, and particularly relates to a drug structural coating having a super-hydrophobic property and a preparation method therefor. The preparation method in the present application uses a phenol amine compound to undergo oxidation, crosslinking and polymerization reactions under the action of an oxidant to generate a polyphenol nanoparticle film layer outside drug-loaded nanoparticles. The polyphenol nano film layer not only serves as a "protective layer" for the drug-loaded nanoparticles and a cross-linking substance between the drug-loaded nanoparticles, but also can immobilize the drug-loaded nanoparticles onto the surface of a substrate material, so that after reaction and deposition, a drug nanometer multi-level structural coating having a micro-nano topological structure can be formed on the surface of the substrate material. During an irradiation process, an ultraviolet cross-linking device can generate a large number of active oxygen free radicals, so that by means of ultraviolet irradiation on a sample, the active oxygen free radicals fully react with a micelle protective layer in the drug coating, so as to convert hydrophilic phenolic hydroxyl in the phenol amine into hydrophobic quinonyl. Therefore, the micro-nano topological structure and the hydrophobic group cooperatively act to endow the surface of the material with the super-hydrophobic property.

Description

A drug structure coating with super-hydrophobic properties and preparation method thereof

[0001] Related applications

[0002] This application claims priority to Chinese patent application No. 202310859596X, filed on July 13, 2023, entitled “A drug structure coating with super-hydrophobic properties and its preparation method,” the entire text of which is hereby incorporated by reference. Technical Field

[0003] The present application belongs to the technical field of biomedical functional materials, and specifically relates to a drug structure coating with super-hydrophobic properties and a preparation method thereof. Background Art

[0004] By studying surfaces with superwetting phenomena in nature, it was found that superhydrophilic and superhydrophobic surfaces are achieved by the synergy of micro / nanotopological structures and chemical groups. According to research, in anti-fouling materials, superhydrophobic surfaces have better resistance and adhesion properties than superhydrophilic surfaces. Superhydrophobic materials are also widely used in self-cleaning, anti-corrosion, flow resistance reduction and other fields besides anti-fouling, and therefore have attracted the attention of scientific researchers and engineering technicians. However, the current research has the following shortcomings:

[0005] 1. At present, researchers use template method, sol-gel method, chemical modification method, self-assembly method, thermal cracking method, chemical deposition method, photolithography method and other process methods based on wetting model theory to biomimetically prepare superhydrophobic material surfaces with different water adhesion forces. Although the required application performance can be achieved, there are still problems such as expensive instruments and reaction raw materials, cumbersome preparation steps, low stability of layered rough structure, severe reaction parameters, damage to the substrate, and difficulty in achieving on the surface of complex structural materials. In addition, the prepared micro-nano topological structures are all constructed of inert substances.

[0006] 2. Specifically in practical applications, for medical anti-fouling materials (especially long-term blood contact materials, such as blood contact catheters, artificial blood vessels, vascular stents, heart valves, etc.), after contact with the human body, the proteins in the body (especially plasma proteins) will quickly adhere to and denature on the material surface, causing acute thrombosis and acute inflammatory reactions, which will have an adverse effect on the service life of the implanted materials. Therefore, the anti-protein adhesion performance of the material in the early stage of implantation plays an important role in preventing acute thrombosis and acute inflammatory reactions. However, as the service time of the device in the body increases, the superhydrophobic layer and the air layer in the body fluid will eventually be destroyed. After the superhydrophobicity is lost, the hydrophobic surface exposed has strong adhesion properties. Proteins in the body fluid will non-specifically adhere and denature on the material surface, causing serious thrombosis and inflammatory reactions. In addition, for devices such as arteriovenous stents, artificial blood vessels, heart valves, etc., although the material surface has certain anti-fouling properties in the early stage, long-term use will also cause problems such as poor anticoagulation, anti-inflammatory, antibacterial and anti-proliferative properties.

[0007] Therefore, based on the above problems at this stage, the technical solution of this application is proposed.

[0008] Summary of the Invention

[0009] Based on this, according to various embodiments of the present application, a drug structure coating with super-hydrophobic properties and a preparation method thereof are provided, and the technical solution is:

[0010] The present application provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0011] (1) dispersing the drug-loaded nanoparticles in a solvent to obtain a drug-loaded nanoparticle suspension;

[0012] (2) mixing the drug-loaded nanoparticle suspension with a phenolamine compound acidic buffer and an oxidant acidic buffer to obtain a mixed solution;

[0013] (3) adding the mixed solution to the surface of the base material to react, and then washing and drying to obtain a modified base material;

[0014] (4) irradiating the modified substrate material with ultraviolet light to obtain the drug structure coating with super-hydrophobic properties.

[0015] To facilitate understanding of the present application, the reaction process of the present application is described below:

[0016] The present application first disperses the drug-loaded nanoparticles uniformly in a solvent, and then, under an acidic environment, oxidizes, crosslinks, and polymerizes the phenolamine nanoparticles (10 nanometers) film layer by a phenolamine compound under the action of an oxidant. The nanofilm layer firmly fixes the drug-loaded nanoparticles (100 nanometers) on the surface of the substrate while uniformly wrapping them; repeatedly depositing by multiple reactions, thereby obtaining a uniform and dense drug nano multi-level structure surface with a micro-nano topological structure. The active oxygen free radicals generated by the ultraviolet irradiation process are then oxidized to the phenolic hydroxyl group in the coating, and the hydrophilic phenolic hydroxyl group is oxidized to a hydrophobic quinone group, which changes the wettability of the coating, and finally forms a drug structure coating with super-hydrophobic performance with the drug as the main component of the coating. This technology can be used for surface modification of materials and devices such as catheters, vascular stents, artificial blood vessels, heart valves, corrosion protection, water / oil separation, etc.

[0017] Preferably, in step (1), the drug-loaded nanoparticles are one of drug-loaded polymer nanoparticles (polymer nanospheres and nanocapsules), drug-loaded nanoliposomes, drug-loaded polymer micelles, drug-loaded dendrimers or drug-loaded inorganic nanoparticles.

[0018] Preferably, in step (1), the solvent is an aqueous solution, an ethanol solution or a mixed solution of water and ethanol; in the mixed solution of water and ethanol, the volume ratio of ethanol to water is 0.1-5:0.1-5.

[0019] Preferably, in step (2), the phenolamine compound acidic buffer solution is a mixture of a phenolamine compound and an acidic buffer solution; the phenolamine compound is one or a combination of two or more of tannic acid, gallic acid, dopamine, salvianolic acid B, epigallocatechin gallate, epicatechin gallate, epicatechin, norepinephrine, aminophenols, epigallocatechin, catechol, and pyrogallol.

[0020] Preferably, in step (2), the oxidant acidic buffer solution is a mixture of an oxidant and an acidic buffer solution; the oxidant is one or a combination of two or more of hydrogen peroxide, ammonium persulfate, copper chloride, concentrated nitric acid, sodium periodate, ferric chloride, potassium permanganate, silver nitrate, and potassium dichromate.

[0021] Preferably, in step (2), the pH of the acidic buffer is 3 to 7; the acidic buffer is one of acetic acid-acetate buffer, 2-(N-morpholinoline)ethanesulfonic acid buffer, glycine-hydrochloric acid buffer, phthalic acid-hydrochloric acid buffer, potassium hydrogen phthalate-sodium hydroxide buffer, disodium hydrogen phosphate-citric acid buffer, citric acid-sodium hydroxide-hydrochloric acid buffer or citric acid-sodium citrate buffer.

[0022] Preferably, in step (2), in the mixed solution, the concentration of the drug-loaded nanoparticles is 0.5-10 mg / mL, the concentration of the phenolamine compound is 0.5-20 mg / mL, and the concentration of the oxidant is 0.1-10 mg / mL.

[0023] Preferably, in step (3), the substrate material is one of a metal-based biomaterial, a ceramic-based biomaterial, a polymer-based biomaterial or a composite biomaterial; the reaction temperature is 10 to 40°C, the reaction time is 0.1 to 10 hours, and the number of reactions is 1 to 10 times.

[0024] Preferably, in step (4), the wavelength of the ultraviolet light irradiation is 365 nm (UVA), 302 nm (UVB) or 254 nm (UVC), and the time of the ultraviolet light irradiation is 1 to 48 h.

[0025] Based on the same technical concept, another solution of the present application is to provide a drug structure coating with super-hydrophobic properties obtained by the above preparation method.

[0026] The beneficial effects of this application include:

[0027] 1. The preparation method described herein, based on the oxidation, cross-linking, polymerization, and deposition of phenolamine compounds in the presence of an oxidant, requires only a simple "one-pot" method to form a uniform polyphenol nanoparticle film on the surface of drug-loaded nanoparticles. This polyphenol nanoparticle film not only increases the stability of the drug-loaded nanoparticles but also firmly anchors them to the substrate surface, expanding the application of drug-loaded nanoparticles on super-hydrophobic surfaces. Furthermore, the polyphenol nanoparticle film is material-independent and can be modified on substrates of varying shapes and properties, thus possessing broad applicability.

[0028] 2. Superhydrophobic surfaces prepared by traditional methods are not biologically active (constructed with inert materials) and rely solely on superhydrophobic properties for passive antifouling. The drug-structured coating described in this application achieves the performance of a bulk material constructed with drugs as the coating. As long as the coating exists, the drugs are present and can exert their biological functions for a long time. The superhydrophobic platform (passive antifouling) and the drugs (active antifouling) synergistically maintain the stability of the material microenvironment, making it possible to achieve the transformation from artificial antifouling surfaces to natural antifouling surfaces.

[0029] 3. Unlike physical dipping and chemical grafting methods for drug loading in coatings, the preparation method described in this application loads the drug into nanoparticles (e.g., polymer micelles), significantly increasing the drug loading capacity of the coating. Furthermore, the prepared drug-loaded nanoparticles are protected by a phenolamine nanofilm layer on their outer surface, effectively protecting the drug's biological activity from destruction during UV irradiation, maintaining the drug's original biological properties.

[0030] 4. This application is simple to operate, has efficient reactions, mild conditions, and broad-spectrum practicality. Different drug-loaded nanoparticles can be modified on substrate surfaces of different materials and shapes to prepare super-hydrophobic drug nano-multilevel structure coatings that have drugs present as long as the coating exists and exert long-lasting biological functions. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0032] FIG1 is a scanning electron microscope (SEM) image of the surface morphology of the drug structure coating with super-hydrophobic properties obtained in Example 1;

[0033] FIG2 is a dynamic water contact angle diagram (a) and a rolling angle diagram (b) of the drug structure coating with super-hydrophobic properties obtained in Example 1;

[0034] FIG3 is a fluorescent staining image of the long-lasting antibacterial effect of the drug structure coating with super-hydrophobic properties prepared in Example 1. DETAILED DESCRIPTION

[0035] To make the purpose, technical solutions, and advantages of this application more clear, the technical solutions of this application will be described in detail below. Obviously, the embodiments described are only some of the embodiments of this application, rather than all of them. Based on the embodiments in this application, all other implementation methods obtained by ordinary technicians in this field without making any creative work are within the scope of protection of this application.

[0036] Example 1

[0037] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0038] (1) dispersing drug-loaded Tween-80 (polymer micelles) in a mixed solvent of ethanol / water to obtain a drug-loaded nanoparticle suspension; wherein the volume ratio of ethanol to water is 1:4;

[0039] (2) dissolving tannic acid and copper chloride in sodium acetate buffer at pH=5 to obtain tannic acid buffer and copper chloride buffer respectively;

[0040] (3) mixing the drug-loaded nanoparticle suspension, the tannic acid buffer, and the copper chloride buffer to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, tannic acid, and copper chloride are 2 mg / mL, 1 mg / mL, and 0.5 mg / mL, respectively;

[0041] (4) Adding the mixed solution to the surface of the clean metal-based biomaterial, reacting at 25°C for 3 h, washing with RO water, repeating the reaction and deposition twice, and then drying with nitrogen to obtain a modified base material;

[0042] (5) The modified substrate material is placed in an ultraviolet cross-linking instrument with a wavelength of 254 nm (the ultraviolet cross-linking instrument will generate a large amount of active oxygen free radicals during the irradiation process) and irradiated for 5 hours to obtain a drug structure coating with super-hydrophobic properties.

[0043] Example 2

[0044] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0045] (1) dispersing drug-loaded polylactic acid nanospheres (polymer nanoparticles) in water to obtain a drug-loaded nanoparticle suspension;

[0046] (2) dissolving dopamine and potassium permanganate in acetic acid-acetate buffer at pH 4 to obtain dopamine buffer and potassium permanganate buffer respectively;

[0047] (3) mixing the drug-loaded nanoparticle suspension, the dopamine buffer, and the potassium permanganate buffer to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, dopamine, and potassium permanganate are 5 mg / mL, 2 mg / mL, and 2 mg / mL, respectively;

[0048] (4) adding the mixed solution to the surface of the clean polymer-based biomaterial, reacting at 20°C for 5 h, then washing with RO water and repeating the reaction and deposition three times, and then drying with nitrogen to obtain a modified base material;

[0049] (5) The modified substrate material is placed in a UV cross-linking instrument with a wavelength of 254 nm and fully irradiated for 6 hours to obtain a drug structure coating with super-hydrophobic properties.

[0050] Example 3

[0051] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0052] (1) dispersing drug-loaded lecithin (solid lipid nanoparticles) in water to obtain a drug-loaded nanoparticle suspension;

[0053] (2) Dissolving epicatechin gallate and ferric chloride in a phthalic acid-hydrochloric acid buffer solution at pH 3.5, respectively, to obtain epicatechin gallate buffer solution and ferric chloride buffer solution;

[0054] (3) mixing the drug-loaded nanoparticle suspension, the epicatechin gallate buffer solution, and the ferric chloride buffer solution to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, epicatechin gallate, and ferric chloride are 6 mg / mL, 3 mg / mL, and 3 mg / mL, respectively;

[0055] (4) Adding the mixed solution to the surface of the clean ceramic-based biomaterial, reacting at 15°C for 10 h, washing with RO water, and repeating the reaction and deposition 5 times, followed by nitrogen drying to obtain a modified base material;

[0056] (5) The modified substrate material is placed in a UV cross-linking instrument with a wavelength of 365 nm and fully irradiated for 8 hours to obtain a drug structure coating with super-hydrophobic properties.

[0057] Example 4

[0058] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0059] (1) dispersing the drug-loaded nanoliposomes in water to obtain a drug-loaded nanoparticle suspension;

[0060] (2) dissolving norepinephrine and ammonium persulfate in a citric acid-sodium citrate buffer solution at pH = 4, respectively, to obtain a norepinephrine buffer solution and an ammonium persulfate buffer solution;

[0061] (3) mixing the drug-loaded nanoparticle suspension, the norepinephrine buffer solution, and the ammonium persulfate buffer solution to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, norepinephrine, and ammonium persulfate are 2 mg / mL, 1 mg / mL, and 1 mg / mL, respectively;

[0062] (4) Add the mixed solution to the surface of the clean metal-based biomaterial, react at 35°C for 1.5 hours, then rinse thoroughly with RO water and repeat the reaction and deposition once, and then dry with nitrogen to obtain a modified base material;

[0063] (5) The modified substrate material is placed in a UV cross-linking instrument with a wavelength of 302 nm and fully irradiated for 2 h to obtain a drug structure coating with super-hydrophobic properties.

[0064] Example 5

[0065] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0066] (1) dispersing drug-loaded porous silica nanoparticles (inorganic nanoparticles) in water to obtain a drug-loaded nanoparticle suspension;

[0067] (2) dissolving epigallocatechin gallate and silver nitrate in a glycine-hydrochloric acid buffer solution at pH 6, respectively, to obtain epigallocatechin gallate buffer solution and silver nitrate buffer solution;

[0068] (3) mixing the drug-loaded nanoparticle suspension, the epigallocatechin gallate buffer solution, and the silver nitrate buffer solution to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, epigallocatechin gallate, and silver nitrate are 6 mg / mL, 2 mg / mL, and 2 mg / mL, respectively;

[0069] (4) adding the mixed solution to the surface of the clean polymer-based biomaterial, reacting at 35°C for 1 hour, then washing with RO water and repeating the reaction and deposition three times, and then drying with nitrogen to obtain a modified base material;

[0070] (5) The modified substrate material is placed in a UV cross-linking instrument with a wavelength of 254 nm and fully irradiated for 4 hours to obtain a drug structure coating with super-hydrophobic properties.

[0071] Example 6

[0072] This embodiment provides a method for preparing a drug structure coating having super-hydrophobic properties, the preparation method comprising the following steps:

[0073] (1) dispersing drug-loaded poloxamer nanoparticles in water to obtain a drug-loaded nanoparticle suspension;

[0074] (2) dissolving dopamine and copper chloride in acetic acid-acetate buffer at pH 6.5 to obtain dopamine buffer and copper chloride buffer respectively;

[0075] (3) mixing the drug-loaded nanoparticle suspension, the dopamine buffer, and the copper chloride buffer to obtain a mixed solution; wherein the concentrations of the drug-loaded nanoparticles, dopamine, and copper chloride are 8 mg / mL, 4 mg / mL, and 4 mg / mL, respectively;

[0076] (4) adding the mixed solution to the surface of the clean polymer-based biomaterial, reacting at 25°C for 2 h, then washing with RO water and repeating the reaction and deposition three times, and then drying with nitrogen to obtain a modified base material;

[0077] (5) The modified substrate material is placed in a UV cross-linking instrument with a wavelength of 302 nm and fully irradiated for 5 h to obtain a drug structure coating with super-hydrophobic properties.

[0078] Experimental example

[0079] The SEM morphology of the drug nano-hierarchical structure coating with super-hydrophobic properties of the product obtained in Example 1 is shown in Figure 1. It can be seen from the figure that a layer of nano-hierarchical structure coating composed mainly of drug-loaded nanoparticles is generated on the surface of the base material. The outer surface of the drug-loaded nanoparticles in the coating is uniformly covered by phenolamine nanoparticles with a particle size of 10 to 20 nm, and the reaction process does not change the morphology of the drug-loaded nanoparticles, and has a nano-hierarchical structure.

[0080] From the water contact angle (a) and water droplet rolling angle test (b) of the coating in Figure 2, it can be seen that the prepared coating has super hydrophobic properties that mimic the lotus leaf structure.

[0081] The water contact angle of the coating surface was measured using a dynamic water contact angle meter. Figure 2 (a) shows the contact state of the water droplet on the sample surface: at 2 seconds (t = 2s), the water droplet is in contact with the sample surface; from 2 seconds to 5 seconds (t = 2s to 5s), the water droplet is in full contact with the surface; from 6 seconds (t = 6s), the water droplet leaves the sample surface. The results show that the water droplet cannot stay on the sample surface, proving that the coating has superhydrophobic properties.

[0082] Figure 2 (b) shows a simulation of a superhydrophobic coating deposited directly on a 9-cm diameter petri dish, measuring a coating roll angle of less than 5 degrees. At 1.25 seconds (t = 1.25s), two water droplets contact the surface; at 1.69 seconds (t = 1.69s), the droplets roll on the coating surface; and between 2.88 and 3.97 seconds (t = 2.88s and 3.97s), the droplets mix and roll on the surface.

[0083] Furthermore, the base material in FIG3 is the metal-based biomaterial described in step (4) of Example 1. After the base material is fully cleaned with anhydrous ethanol and water, it is directly cultured with Staphylococcus aureus as a control. Then, the drug nano-multilevel structure coating with super-hydrophobic properties of the product obtained in Example 1 is directly cultured with Staphylococcus aureus (0D), and then the drug nano-multilevel structure coating with super-hydrophobic properties of the product obtained in Example 1 is immersed in a 1 mg / mL bovine serum albumin solution diluted with an alkaline phosphate solution for 7 days (7D) and 14 days (14D). After being fully cleaned, they are cultured with Staphylococcus aureus for 6 hours, and after DAPI staining, the surface bacterial adhesion is observed by laser confocal microscopy.

[0084] As can be seen from Figure 3, a large number of bacteria adhered to the surface of the substrate; however, virtually no bacteria adhered to any of the superhydrophobic surfaces treated according to the method described in Example 1. This indicates that the superhydrophobic drug nanostructured coating can effectively inhibit the adhesion and growth of proteins and bacteria.

[0085] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A method for preparing a drug structure coating having super-hydrophobic properties, characterized in that: The preparation method comprises the following steps: (1) dispersing the drug-loaded nanoparticles in a solvent to obtain a drug-loaded nanoparticle suspension; (2) mixing the drug-loaded nanoparticle suspension with a phenolamine compound acidic buffer and an oxidant acidic buffer to obtain a mixed solution; (3) adding the mixed solution to the surface of the base material to react, and after completion, washing and drying to obtain a modified base material; and (4) The modified substrate material is irradiated with ultraviolet light to obtain the drug structure coating with super-hydrophobic properties.

2. The method for preparing a drug structure coating having super-hydrophobic properties according to claim 1, characterized in that: In step (1), the drug-loaded nanoparticles are one of drug-loaded polymer nanoparticles, drug-loaded nanoliposomes, drug-loaded polymer micelles, drug-loaded dendrimer macromolecules or drug-loaded inorganic nanoparticles.

3. The method for preparing a drug structure coating having super-hydrophobic properties according to claim 1 or 2, characterized in that: In step (1), the solvent is an aqueous solution, an ethanol solution, or a mixed solution of water and ethanol; In the mixed solution of water and ethanol, the volume ratio of ethanol to water is about 0.1-5:0.1-5.

4. The method for preparing a drug structure coating having super-hydrophobic properties according to any one of claims 1 to 3, characterized in that: In step (2), the phenolamine compound acidic buffer solution is formed by mixing a phenolamine compound and an acidic buffer solution; The phenolamine compound is one or a combination of two or more of tannic acid, gallic acid, dopamine, salvianolic acid B, epigallocatechin gallate, epicatechin gallate, epicatechin, norepinephrine, aminopolyphenols, epigallocatechin, catechol and pyrogallol.

5. The method for preparing a drug structure coating having super-hydrophobic properties according to any one of claims 1 to 4, characterized in that: In step (2), the oxidant acidic buffer solution is formed by mixing an oxidant and an acidic buffer solution; The oxidant is one or a combination of two or more of hydrogen peroxide, ammonium persulfate, cupric chloride, concentrated nitric acid, sodium periodate, ferric chloride, potassium permanganate, silver nitrate and potassium dichromate.

6. The method for preparing a drug structure coating having super-hydrophobic properties according to claim 4 or 5, characterized in that: The pH of the acidic buffer is about 3 to 7; The acidic buffer is one of acetic acid-acetate buffer, 2-(N-morpholine)ethanesulfonic acid buffer, glycine-hydrochloric acid buffer, phthalic acid-hydrochloric acid buffer, potassium hydrogen phthalate-sodium hydroxide buffer, disodium hydrogen phosphate-citric acid buffer, citric acid-sodium hydroxide-hydrochloric acid buffer or citric acid-sodium citrate buffer.

7. The method for preparing a drug structure coating having super-hydrophobic properties according to any one of claims 1 to 6, characterized in that: In step (2), in the mixed solution, the concentration of the drug-loaded nanoparticles is about 0.5-10 mg / mL, the concentration of the phenolamine compound is about 0.5-20 mg / mL, and the concentration of the oxidant is about 0.1-10 mg / mL.

8. The method for preparing a drug structure coating having super-hydrophobic properties according to any one of claims 1 to 7, characterized in that: In step (3), the substrate material is one of a metal-based biomaterial, a ceramic-based biomaterial, a polymer-based biomaterial and a composite biomaterial; The reaction temperature is about 10-40° C., the reaction time is about 0.1-10 h, and the reaction times are about 1-10 times.

9. The method for preparing a drug structure coating having super-hydrophobic properties according to any one of claims 1 to 8, characterized in that: In step (4), the wavelength of the ultraviolet light irradiation is about 365 nm, about 302 nm or about 254 nm, and the time of the ultraviolet light irradiation is about 1 to 48 h.

10. The drug structure coating with super-hydrophobic properties obtained by the preparation method according to any one of claims 1 to 9.

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