Modified precursor of RNA interference-inducing nucleic acid molecule comprising structural motif promoting drosha cleavage

By inserting a GAG or GUG motif in the 3p strand of pri-miRNA precursors to form a bulge, the modified precursor enhances Drosha recognition, addressing inaccuracies in pri-miRNA structure prediction and improving RNA interference efficiency.

WO2025178406A1 Publication Date: 2025-08-28SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION +1
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Patent Information

Application Number
PCT/KR2025/002495
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-02-21
Filing Date
2025-02-21
Publication Date
2025-08-28

AI Technical Summary

Technical Problem

Current methods for predicting the secondary structures of pri-miRNAs are inaccurate, leading to uncertainties in understanding miRNA biogenesis, regulation, and evolution, as computational methods often misestimate stem lengths and apical loop structures, resulting in inconsistent processing efficiency and accuracy.

Method used

A modified precursor of RNA interference-inducing nucleic acid molecules is introduced, featuring a GAG or GUG motif inserted in the 3p strand near the Drosha cleavage site, forming a bulge, to enhance Drosha recognition and processing efficiency.

Benefits of technology

The modified precursor leads to higher expression levels of RNA interference-inducing nucleic acid molecules by optimizing Drosha cleavage, improving processing accuracy and homogeneity.

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Abstract

The present invention relates to a novel modified precursor of an RNA interference-inducing nucleic acid molecule, to a method for designing the modified precursor, and to a method for producing an RNA interference-inducing nucleic acid molecule. A modified precursor, according to one aspect, can be efficiently and homogeneously processed, and thus exhibits a higher expression level in cells, thereby being useful for design and regulation studies of RNA interference-inducing nucleic acid molecules.
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