Gold and silver combined inspection test paper for feline and canidae animals important pestilence pathogen and its production

Abstract

A colloidal gold-silver united test paper used for detecting etiology of important disease on Felidae and Canidae animal is prepared mix-enveloping mouse source RV, CDV, RPV and CPV single-anti being labeled with colloidal gold on glass cellulose membrane to form combination pad; enveloping IgG of purified dog-resisting RV, CDV, FPV and CPV on R, D, F and P positions of detection line at nitric acid cellulose membrane and enveloping rabbit resisting mouse IgG on C position of quality control line at nitric acid cellulose membrane to from detection membrane.

Description

Technical field:

[0001] The invention relates to a colloidal gold-silver joint detection test paper for important epidemic diseases of dogs and felines and a preparation method thereof, which is used for rapid detection of important diseases such as rabies, canine distemper, feline distemper and canine parvovirus in dogs and felines. Detection and diagnosis belong to the technical field of animal epidemic detection. Background technique:

[0002] Canine rabies, canine distemper, feline distemper and canine parvovirus and other important diseases of dogs and cats not only seriously endanger my country's fur economic animal industry, but also seriously threaten the health of tigers, lions, wolves and other rare wild animals , and some can lead to human morbidity and death. To fundamentally control these diseases, the most important thing is to make timely and accurate monitoring of the occurrence and prevalence of the above-mentioned diseases. For the important epidemic disea...

Examples

Embodiment 1

[0014] According to shown in Figure 1, the structure of the detection test paper of the present invention is as follows:

[0015] The liner 8 is enveloped in the plastic clip 1, and the surface of the plastic clip 1 is provided with a sampling hole 2 and an observation hole 3, and the surface of the liner 8 is assembled with a processed sample pad 4, a binding pad 5, a detection film 6 and an absorbent. Pad 7. Among them, the binding pad 5 is made of glass cellulose membrane, coated with mouse monoclonal antibodies such as gold-labeled RV, CDV, FPV, and CPV; the detection membrane 6 is made of nitrocellulose membrane, and there are multiple detection points on the upper spray point. Line and 1 quality control line, R, D, F, P, etc. are detection lines, coated with purified dog anti-RV, CDV, FPV and CPV IgG; C is the quality control line, coated with rabbit anti-mouse II Resistance; the detection membrane is connected to the absorbent pad 7 .

Embodiment 2

[0017] 1) Preparation of RV, CDV, FPV, CPV and other pathogenic monoclonal antibodies and polyantibody IgG: Inoculate sensitive cells with RV, CDV, FPV and CPV and other pathogens respectively, culture and proliferate each virus, and immunize Balb / c respectively after concentration and purification For mice and domestic dogs, prepare monoclonal antibody and polyantibody serum for each virus, and further prepare the monoclonal antibody and polyantibody IgG, and store them at -30°C for future use.

[0018] 2) Preparation of RV, CDV, FPV and CPV and other pathogenic solid-phase immunosorbent carriers: apply the prepared RV, CDV, FPV and CPV and other pathogenic polyantibody IgG coated magnetic beads, latex particles and other solid-phase carriers, respectively prepare the above The high-efficiency immunoadsorption carrier of the pathogen is mixed in an appropriate proportion to make a combined pathogen solid-phase immunoadsorption carrier, which is added with preservatives and sto...

Technical field:

[0001] The invention relates to a colloidal gold-silver joint detection test paper for important epidemic diseases of dogs and felines and a preparation method thereof, which is used for rapid detection of important diseases such as rabies, canine distemper, feline distemper and canine parvovirus in dogs and felines. Detection and diagnosis belong to the technical field of animal epidemic detection. Background technique:

[0002] Canine rabies, canine distemper, feline distemper and canine parvovirus and other important diseases of dogs and cats not only seriously endanger my country's fur economic animal industry, but also seriously threaten the health of tigers, lions, wolves and other rare wild animals , and some can lead to human morbidity and death. To fundamentally control these diseases, the most important thing is to make timely and accurate monitoring of the occurrence and prevalence of the above-mentioned diseases. For the important epidemic disea...

Examples

Embodiment 1

[0014] According to shown in Figure 1, the structure of the detection test paper of the present invention is as follows:

[0015] The liner 8 is enveloped in the plastic clip 1, and the surface of the plastic clip 1 is provided with a sampling hole 2 and an observation hole 3, and the surface of the liner 8 is assembled with a processed sample pad 4, a binding pad 5, a detection film 6 and an absorbent. Pad 7. Among them, the binding pad 5 is made of glass cellulose membrane, coated with mouse monoclonal antibodies such as gold-labeled RV, CDV, FPV, and CPV; the detection membrane 6 is made of nitrocellulose membrane, and there are multiple detection points on the upper spray point. Line and 1 quality control line, R, D, F, P, etc. are detection lines, coated with purified dog anti-RV, CDV, FPV and CPV IgG; C is the quality control line, coated with rabbit anti-mouse II Resistance; the detection membrane is connected to the absorbent pad 7 .

Embodiment 2

[0017] 1) Preparation of RV, CDV, FPV, CPV and other pathogenic monoclonal antibodies and polyantibody IgG: Inoculate sensitive cells with RV, CDV, FPV and CPV and other pathogens respectively, culture and proliferate each virus, and immunize Balb / c respectively after concentration and purification For mice and domestic dogs, prepare monoclonal antibody and polyantibody serum for each virus, and further prepare the monoclonal antibody and polyantibody IgG, and store them at -30°C for future use.

[0018] 2) Preparation of RV, CDV, FPV and CPV and other pathogenic solid-phase immunosorbent carriers: apply the prepared RV, CDV, FPV and CPV and other pathogenic polyantibody IgG coated magnetic beads, latex particles and other solid-phase carriers, respectively prepare the above The high-efficiency immunoadsorption carrier of the pathogen is mixed in an appropriate proportion to make a combined pathogen solid-phase immunoadsorption carrier, which is added with preservatives and sto...

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