A kind of forensic medicine diatom test method

Abstract

The invention relates to the field of forensic medical inspection, and relates to a forensic medical diatom inspection method. It at least includes the following steps: (1) Microwave digestion: add concentrated nitric acid and hydrogen peroxide solution to the test sample and then carry out microwave digestion; (2) Vacuum suction filtration: carry out vacuum suction filtration on the digestion solution, and then Add ultra-pure water to continue the suction filtration to make the surface of the filter membrane close to neutral, and finally add absolute ethanol to remove the water in the filter membrane by suction filtration; (3) Scanning electron microscope automatically takes pictures and stores the images (5) Qualitative and quantitative analysis of diatoms: using manual identification Check, classify and statistically process the diatoms in the captured field of view images by means of automatic recognition by computer. The invention has high detection sensitivity, can effectively avoid pollution, is simple, efficient, environmentally friendly, and has accurate qualitative and quantitative analysis, greatly improves the working environment of forensic diatom inspection technicians, reduces labor intensity, and has good application in the practice of forensic drowning diagnosis prospect.

Description

technical field

[0001] The invention relates to the field of forensic medical inspection, in particular to a forensic medical diatom inspection method for identifying the cause of death of corpses in water by inspecting the diatom species and content in organs, tissues and organs. Background technique

[0002] The cause of death analysis of corpses in water has always been one of the difficulties in the practice of forensic medicine. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter various organs of the large circulation. Although there are still differences in the diagnostic value of diatom detection in theory, in forensic practice, diatom detection is still considered to be a relatively reliable means of diagnosing drowning, especially for the identification of the cause of death of decayed corpses in water. good identification method. Under the condition that pollution can be prevented, more diatoms of the same...

Examples

Embodiment 1

[0044] Embodiment one, experimental embodiment of the present invention

[0045] 1. Experimental materials and equipment

[0046] 30 New Zealand white rabbits, male or female, weighing 1.0-3.0 kg, were provided by the Animal Experiment Center of Guangdong Province, and were randomly divided into two groups: drowning group (n=15) and postmortem submersion group (n=15). The central location of Haizhu Bridge in Guangzhou City on the Pearl River was selected as the experimental site. Put the rabbits in the drowning group in a rabbit cage, sink them into the water at 0.5m, lift them out of the water after 1min, and sink them back into the same water depth after 30s, repeat several times to slowly drown the white rabbits, soak them in water for 30min after death, and put them into the water after death The experimental rabbits in the group were killed by air embolism, placed in the same place and soaked at the same depth for 30 minutes, and then taken out. Extract the lung, liver,...

Embodiment 2

[0122] Typical criminal case 1: A 3-year-old girl named Xie disappeared, and her body was found in a cistern in a residential area 3 months later. Due to the high degree of corruption of the corpse, it is difficult to determine the cause of death and the nature of the case after the autopsy. The diatom test was digested by nitric acid ethanol method and observed by optical microscope. As a result, the same type of diatoms as in the reservoir was detected from the lungs, the content was 550 / 10g lung tissue, and the diatom test in liver and kidney tissues was negative. The MW-VF-Auto SEM method was used to detect the same type of diatoms from the lungs, liver, and kidneys of the deceased as in the water samples of the reservoir, and the contents were 720 / 10g lung tissue and 3 / 10g liver tissue respectively. , 2 / 10g kidney tissue, so the cause of death was diagnosed as drowning. Combined with other investigation results, it was finally confirmed that the deceased died by accident...

Embodiment 3

[0124] Typical criminal case 2: The victim Huang was kidnapped and extorted 1 million yuan. His highly decomposed body was found in a lake in the suburbs 10 days later. In order to confirm whether the deceased was thrown into the water before death or was thrown into the water after death, the lung, liver and kidney tissues were collected after autopsy and digested with nitric acid ethanol. , only the same type of diatoms as in the on-site water samples were detected in the lungs, the content was 2030 / 10g lung tissue, and the diatoms in the liver and kidney tissues were negative, so a clear diagnosis of drowning could not be made. Afterwards, the MW-VF-Auto SEM method was used to detect the same type of diatoms as the on-site water samples from the lungs and livers. The content of diatoms in the lung tissue was 2580 per 10g of lung tissue, and the content of diatoms in the liver tissue It was 5 / 10g liver tissue, and according to the conclusion of this test, the cause of death...

technical field

[0001] The invention relates to the field of forensic medical inspection, in particular to a forensic medical diatom inspection method for identifying the cause of death of corpses in water by inspecting the diatom species and content in organs, tissues and organs. Background technique

[0002] The cause of death analysis of corpses in water has always been one of the difficulties in the practice of forensic medicine. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter various organs of the large circulation. Although there are still differences in the diagnostic value of diatom detection in theory, in forensic practice, diatom detection is still considered to be a relatively reliable means of diagnosing drowning, especially for the identification of the cause of death of decayed corpses in water. good identification method. Under the condition that pollution can be prevented, more diatoms of the same...

Examples

Embodiment 1

[0044] Embodiment one, experimental embodiment of the present invention

[0045] 1. Experimental materials and equipment

[0046] 30 New Zealand white rabbits, male or female, weighing 1.0-3.0 kg, were provided by the Animal Experiment Center of Guangdong Province, and were randomly divided into two groups: drowning group (n=15) and postmortem submersion group (n=15). The central location of Haizhu Bridge in Guangzhou City on the Pearl River was selected as the experimental site. Put the rabbits in the drowning group in a rabbit cage, sink them into the water at 0.5m, lift them out of the water after 1min, and sink them back into the same water depth after 30s, repeat several times to slowly drown the white rabbits, soak them in water for 30min after death, and put them into the water after death The experimental rabbits in the group were killed by air embolism, placed in the same place and soaked at the same depth for 30 minutes, and then taken out. Extract the lung, liver,...

Embodiment 2

[0122] Typical criminal case 1: A 3-year-old girl named Xie disappeared, and her body was found in a cistern in a residential area 3 months later. Due to the high degree of corruption of the corpse, it is difficult to determine the cause of death and the nature of the case after the autopsy. The diatom test was digested by nitric acid ethanol method and observed by optical microscope. As a result, the same type of diatoms as in the reservoir was detected from the lungs, the content was 550 / 10g lung tissue, and the diatom test in liver and kidney tissues was negative. The MW-VF-Auto SEM method was used to detect the same type of diatoms from the lungs, liver, and kidneys of the deceased as in the water samples of the reservoir, and the contents were 720 / 10g lung tissue and 3 / 10g liver tissue respectively. , 2 / 10g kidney tissue, so the cause of death was diagnosed as drowning. Combined with other investigation results, it was finally confirmed that the deceased died by accident...

Embodiment 3

[0124] Typical criminal case 2: The victim Huang was kidnapped and extorted 1 million yuan. His highly decomposed body was found in a lake in the suburbs 10 days later. In order to confirm whether the deceased was thrown into the water before death or was thrown into the water after death, the lung, liver and kidney tissues were collected after autopsy and digested with nitric acid ethanol. , only the same type of diatoms as in the on-site water samples were detected in the lungs, the content was 2030 / 10g lung tissue, and the diatoms in the liver and kidney tissues were negative, so a clear diagnosis of drowning could not be made. Afterwards, the MW-VF-Auto SEM method was used to detect the same type of diatoms as the on-site water samples from the lungs and livers. The content of diatoms in the lung tissue was 2580 per 10g of lung tissue, and the content of diatoms in the liver tissue It was 5 / 10g liver tissue, and according to the conclusion of this test, the cause of death...

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