Method for detecting mononucleotide polymorphism of scalper SREBP1c gene

A single nucleotide polymorphism, gene technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of research and blank, and achieve the effect of excellent genetic resources

CN101875977AInactive Publication Date: 2010-11-03NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2010-11-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting mononucleotide polymorphism of a scalper SREBP1c gene, which comprises the following steps of: using a scalper whole genome DNA to be detected containing the SREBP1c gene as a template, using a primer pair P as a primer, and performing PCR amplification on the scalper SREBP1c gene; using a restriction endonuclease Bg1 I to digest a PCR amplification product, and then performing sepharose gel electrophoresis on an amplified fragment after enzyme digestion; and identifying the mononucleotide polymorphism of the 10914th bit of the scalper SREBP1c gene according to the result of the sepharose gel electrophoresis. Because the functions of the SREBP1c gene relate to four important growth traits of body weight, daily gain, body length and chest circumference, the detection method provided by the invention lays a good foundation for establishing a relation between SNP and the growth traits of the SREBP1c gene, and is convenient to be used in a marker assistant selection (MAS) of the growth traits of Chinese scalper beef to quickly establish a scalper population with an excellent genetic resource.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle SREBP1c (sterol regulatory element binding protein 1c) gene. Background technique

[0002] Gene polymorphism refers to the differences in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, mainly including base substitutions, insertions, deletions and duplications. Changes in sequence copy number.

[0003] Single Nucleotide Polymorphism (SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism due to acid (A / T / C / G) substitution. Its variant forms are: transversion, conversion, insertion and deletion, etc., ma...

Examples

Embodiment Construction

[0029] The invention detects the single nucleotide polymorphism of the 10914th position missense mutation of the yellow cattle SREBP1c gene that may produce a change in the conformation of the encoded protein, so as to be used for the marker-assisted selection of the growth traits of Chinese yellow beef, and rapidly establish genetic resources Excellent cattle population. The present invention will be further described in detail below in conjunction with the detection of specific samples and the correlation of traits, which are to explain rather than limit the present invention.

[0030] a. Design of PCR primers containing nt10914 mutation in cattle SREBP1c gene

[0031]Taking the sequence of Hereford cattle (NC_NC_007317.3) published by NCBI as a reference, using Primer5.0 to design PCR primers that can amplify the region of exon 9 of cattle SREBP1c gene, the primer sequences are as follows:

[0032] Upstream primer F1: cgact acatc cgctt cctt 19;

[0033] Downstream primer ...