Porphyra polysaccharide as well as preparation method and application thereof

Abstract

The invention discloses a porphyra polysaccharide which is prepared by the following method steps: smashing porphyra and extracting for 2-5 hours in water with pH value of 1-5 and temperature of 100-120 DEG C; filtering, regulating the pH value of extract to 6.3-6.7 with potassium-containing alkali, and concentrating the extract until the content of solid substances is 40-50%, so as to obtain a concentrated liquid; adding ethanol in the concentrated liquid for evenly mixing, and then standing for more than 8 hours at the temperature of 4 DEG C so as to obtain polysaccharide precipitate; and carrying out re-dissolution and freeze drying on the polysaccharide precipitate, so as to obtain the porphyra polysaccharide. The porphyra polysaccharide the mass of which is 0.01-1.0% of pipe tobaccosis prepared into a solution, and then the porphyra polysaccharide solution is added to the pipe tobaccos or a filter tip through a spraying mode, thereby effectively reducing the content of free radical in main stream smoke of cigarette and also improving the smoking quality of the cigarette. The porphyra polysaccharide has the functions of reducing stimulation effect on nasal cavity, throat and the like, leading smoke to be smooth, maintaining aroma and improving the quality of tobacco products, and has no any side effects. The porphyra polysaccharide has high extraction efficiency and low cost, is easy to obtain and can meet industrial application.

Description

technical field

[0001] The invention relates to the field of extracting effective components of marine plants, in particular to a laver polysaccharide and its preparation method and application. Background technique

[0002] Laver (Porphyra) is a marine plant with high edible and medicinal value. It contains a variety of active substances beneficial to the human body. It has been used in traditional Chinese medicine for thousands of years. There are related records in "Compendium of Materia Medica", which has The effect of "invigorating the kidney and nourishing the heart, diuresis and detumescence". Laver is a food resource with great potential.

[0003] Porphyra polysaccharide is one of the main components of laver. It is a partially sulfated natural linear polysaccharide extracted from laver. It has various activities, including anti-oxidation, anti-radiation, anti-cancer, enhancing cellular immunity and humoral immunity. . At present, the relevant domestic researches ...

Examples

Embodiment 1

[0035] (1) Extraction: After pulverizing laver into 20-mesh laver powder, take 50g and place it in a container, mix it with deionized water in a weight ratio of 1:30, adjust the pH value of the solution to 2.0 with citric acid, and extract 3 Hour.

[0036] (2) Concentration: adjust the pH value of the solution to 6.5 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 40%.

[0037] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 80% of the volume fraction of the mixture, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0038] (4) Drying: Redissolve the polysaccharide precipitate with deionized water, and freeze-dry to obtain the target product, which is immediately stored in a desiccator, ...

Embodiment 2

[0041] (1) Extraction: After crushing laver into 40-mesh laver powder, take 50g and put it in a container, mix it with deionized water in a weight ratio of 1:25, adjust the pH value of the solution to 2.0 with sulfuric acid, and extract it in an oil bath at 110°C 4 hours.

[0042] (2) Concentration: adjust the pH value of the solution to 6.4 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 45%.

[0043] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 75% of the volume fraction of the mixture, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0044] (4) Drying: the polysaccharide precipitate was redissolved with deionized water, and freeze-dried to obtain the target product, which was imm...

Embodiment 3

[0047] (1) Extraction: After crushing laver into 60-mesh laver powder, take 50g and put it in a container, mix it with deionized water at a weight ratio of 1:35, adjust the pH value of the solution to 1.5 with hydrochloric acid, and extract in an oil bath at 100°C 5 hours.

[0048] (2) Concentration: adjust the pH value of the solution to 6.6 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 48%.

[0049] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 85% of the integral fraction of the mixed liquid, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0050] (4) Drying: the polysaccharide precipitate was redissolved with deionized water, and freeze-dried to obtain the target product, which...

technical field

[0001] The invention relates to the field of extracting effective components of marine plants, in particular to a laver polysaccharide and its preparation method and application. Background technique

[0002] Laver (Porphyra) is a marine plant with high edible and medicinal value. It contains a variety of active substances beneficial to the human body. It has been used in traditional Chinese medicine for thousands of years. There are related records in "Compendium of Materia Medica", which has The effect of "invigorating the kidney and nourishing the heart, diuresis and detumescence". Laver is a food resource with great potential.

[0003] Porphyra polysaccharide is one of the main components of laver. It is a partially sulfated natural linear polysaccharide extracted from laver. It has various activities, including anti-oxidation, anti-radiation, anti-cancer, enhancing cellular immunity and humoral immunity. . At present, the relevant domestic researches ...

Examples

Embodiment 1

[0035] (1) Extraction: After pulverizing laver into 20-mesh laver powder, take 50g and place it in a container, mix it with deionized water in a weight ratio of 1:30, adjust the pH value of the solution to 2.0 with citric acid, and extract 3 Hour.

[0036] (2) Concentration: adjust the pH value of the solution to 6.5 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 40%.

[0037] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 80% of the volume fraction of the mixture, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0038] (4) Drying: Redissolve the polysaccharide precipitate with deionized water, and freeze-dry to obtain the target product, which is immediately stored in a desiccator, ...

Embodiment 2

[0041] (1) Extraction: After crushing laver into 40-mesh laver powder, take 50g and put it in a container, mix it with deionized water in a weight ratio of 1:25, adjust the pH value of the solution to 2.0 with sulfuric acid, and extract it in an oil bath at 110°C 4 hours.

[0042] (2) Concentration: adjust the pH value of the solution to 6.4 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 45%.

[0043] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 75% of the volume fraction of the mixture, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0044] (4) Drying: the polysaccharide precipitate was redissolved with deionized water, and freeze-dried to obtain the target product, which was imm...

Embodiment 3

[0047] (1) Extraction: After crushing laver into 60-mesh laver powder, take 50g and put it in a container, mix it with deionized water at a weight ratio of 1:35, adjust the pH value of the solution to 1.5 with hydrochloric acid, and extract in an oil bath at 100°C 5 hours.

[0048] (2) Concentration: adjust the pH value of the solution to 6.6 with potassium hydroxide, centrifuge at 3000 rpm for 6 minutes in a centrifuge, remove the laver residue to obtain an extract, and concentrate the extract to a solid content of 48%.

[0049] (3) Alcohol precipitation: add absolute ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 85% of the integral fraction of the mixed liquid, and stand at 4° C. for 12 hours. Centrifuge at 4800 rpm for 30 min, remove the supernatant, and precipitate polysaccharides.

[0050] (4) Drying: the polysaccharide precipitate was redissolved with deionized water, and freeze-dried to obtain the target product, which...

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