Preparation method and application of ethanol extract of laminarin

Abstract

The invention discloses a preparation method and an application of an ethanol extract of laminarin. The ethanol extract of laminarin is obtained through the preparation method which comprises the following steps: 1, crushing sea tangle, adding an ethanol solution to the crushed sea tangle, extracting for 2-5h at 50-80DEG C; 2, filtering to remove a residue, and adding active carbon to an extract to decolor and remove a fishy smell; and 3, filtering, and concentrating to obtain the ethanol extract of laminarin. The ethanol extract of laminarin is added to cut tobaccos or filter tips in a sprinkling mode, and the mass of the ethanol extract of laminarin accounts for 0.01-1.0% of the mass of cut tobaccos, so the content of free radicals in a mainstream smoke of cigarettes is effectively reduced, the reduction amplitude of the content of the free radicals is more than 15%, and the smoking quality of the cigarettes is improved, thereby the ethanol extract of laminarin, which has irritationreduction effects on nasal cavities, throats and the like, allows the smoke to be smooth, the taste to be soft, the fragrance to be kept, and no side effects to be possessed. The ethanol extract of laminarin has the advantages of no obviously fishy smell, easy obtaining, and low cost.

Description

technical field

[0001] The invention relates to the field of extraction of effective components of marine plants, in particular to a preparation method and application of laminarin ethanol extract. Background technique

[0002] Epidemiological surveys show that smoking can cause many human diseases, such as lung cancer, bronchitis, emphysema, oral cancer, bladder cancer, etc., and free radicals are one of the important causes of various diseases. During the smoking process, the smoke will produce alkoxy radicals RO·, alkyl radicals R·, quinone / semiquinone free radicals, polycyclic aromatic hydrocarbon free radicals, etc., which directly or indirectly attack cell components and cause certain damage to the body. As smoking and health issues have attracted more and more attention, reducing the free radical content in cigarette smoke has become one of the key research topics for cigarette harm reduction. The development and utilization of efficient and non-toxic free radical sca...

Examples

Embodiment 1

[0023] (1) After the kelp is crushed into 10-mesh kelp powder, 50 g is placed in a container, mixed evenly with 50% by weight ethanol solution at a weight ratio of 1:20, and extracted in an oil bath at 50° C. for 3 hours;

[0024] (2) Remove the residue by filtration, add 1% by weight of activated carbon to the extract, and process it at 50°C for 1 hour;

[0025] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 1#.

[0026] The IC50 of laminarin ethanol extract 1# in DPPH·free radical system is 0.48mg / mL.

Embodiment 2

[0028] (1) After the kelp is crushed into 40 mesh kelp powder, 50 g is taken and placed in a container, mixed evenly with 60% by weight ethanol solution at a weight ratio of 1:25, and extracted in an oil bath at 70° C. for 4 hours;

[0029] (2) Remove the residue by filtration, add 2% by weight of activated carbon to the extract, and process it at 55° C. for 1 hour;

[0030] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 2#.

[0031] The IC50 of laminarin ethanol extract 2# in DPPH·free radical system is 0.24mg / mL.

Embodiment 3

[0033] (1) After the kelp is crushed into 60-mesh kelp powder, 50 g is placed in a container, mixed evenly with 70% by weight ethanol solution at a weight ratio of 1:30, and extracted in an oil bath at 70° C. for 4 hours;

[0034] (2) Remove the residue by filtration, add 1% by weight of activated carbon to the extract, and process it at 60° C. for 1 hour;

[0035] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 3#.

[0036] The IC50 of laminarin ethanol extract 3# in DPPH·free radical system is 0.30mg / mL.

technical field

[0001] The invention relates to the field of extraction of effective components of marine plants, in particular to a preparation method and application of laminarin ethanol extract. Background technique

[0002] Epidemiological surveys show that smoking can cause many human diseases, such as lung cancer, bronchitis, emphysema, oral cancer, bladder cancer, etc., and free radicals are one of the important causes of various diseases. During the smoking process, the smoke will produce alkoxy radicals RO·, alkyl radicals R·, quinone / semiquinone free radicals, polycyclic aromatic hydrocarbon free radicals, etc., which directly or indirectly attack cell components and cause certain damage to the body. As smoking and health issues have attracted more and more attention, reducing the free radical content in cigarette smoke has become one of the key research topics for cigarette harm reduction. The development and utilization of efficient and non-toxic free radical sca...

Examples

Embodiment 1

[0023] (1) After the kelp is crushed into 10-mesh kelp powder, 50 g is placed in a container, mixed evenly with 50% by weight ethanol solution at a weight ratio of 1:20, and extracted in an oil bath at 50° C. for 3 hours;

[0024] (2) Remove the residue by filtration, add 1% by weight of activated carbon to the extract, and process it at 50°C for 1 hour;

[0025] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 1#.

[0026] The IC50 of laminarin ethanol extract 1# in DPPH·free radical system is 0.48mg / mL.

Embodiment 2

[0028] (1) After the kelp is crushed into 40 mesh kelp powder, 50 g is taken and placed in a container, mixed evenly with 60% by weight ethanol solution at a weight ratio of 1:25, and extracted in an oil bath at 70° C. for 4 hours;

[0029] (2) Remove the residue by filtration, add 2% by weight of activated carbon to the extract, and process it at 55° C. for 1 hour;

[0030] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 2#.

[0031] The IC50 of laminarin ethanol extract 2# in DPPH·free radical system is 0.24mg / mL.

Embodiment 3

[0033] (1) After the kelp is crushed into 60-mesh kelp powder, 50 g is placed in a container, mixed evenly with 70% by weight ethanol solution at a weight ratio of 1:30, and extracted in an oil bath at 70° C. for 4 hours;

[0034] (2) Remove the residue by filtration, add 1% by weight of activated carbon to the extract, and process it at 60° C. for 1 hour;

[0035] (3) filtering to remove activated carbon, concentrating to a dry matter content of 5% to 10%, and obtaining the laminarin ethanol extract. The obtained product number is laminarin ethanol extract 3#.

[0036] The IC50 of laminarin ethanol extract 3# in DPPH·free radical system is 0.30mg / mL.

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