Portable glucose test tube

Abstract

The invention discloses a portable test tube for detecting glucose. A hydrogel is used as a carrier, the glucose is oxidized by glucose oxidase and generated into hydrogen peroxide, and then, a glucose sample is detected through the chromogenic reaction, which is catalyzed by molybdenum disulfide, between the hydrogen peroxide and a color developing agent. The problems in the prior art, such as high requirements on glucose detecting operation, complicated detection process, high detecting cost, long detecting time, big background interference can be solved. The glucose test tube has the advantages of low cost, simplicity in operation, capability of visually and rapidly detecting the glucose content in the sample, and convenience in carry and storage.

Description

technical field

[0001] The invention belongs to the technical field of glucose detection, in particular to a portable test tube for detecting glucose. Background technique

[0002] In recent years, due to the influence of many factors such as changes in diet structure, increasingly tense life rhythm and less active and more sedentary lifestyle, the incidence of diabetes worldwide has increased rapidly, making diabetes the third serious threat after tumors and cardiovascular diseases Chronic diseases of human health. The detection of glucose in serum is the main means of clinical examination and monitoring of diabetes. At present, most of the device inventions for detecting glucose are electrochemical sensors (patent number: 01810317.0; utility model patent: 200620110322.2; patent number: 201210116431.5; patent number: 201210189645.5; patent number: 201210416267.X; patent number: 201310087177.5; ). The core of these devices is the preparation of functionalized electrodes, ...

Examples

preparation example Construction

[0015] The preparation method mainly includes the following steps: adding molybdenum disulfide solution, chromogen and glucose oxidase solution into the hydrogel solution, mixing evenly, and then transferring the mixed solution to a small groove inside the cover of the centrifuge tube. Under the condition of standing for 5 minutes, make it solidify into a gel, and obtain the glucose test tube.

[0016] The hydrogel includes agar hydrogel, agarose hydrogel or chitosan hydrogel.

[0017] The developer includes 3,3',5,5'-tetramethylbenzidine, 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt or o- phenylenediamine.

[0018] The detection method is to dilute the glucose sample and add it to the bottom of the test tube, cover the lid and turn it upside down, let the sample solution contact with the gel for a period of time, and then semi-quantitatively detect the glucose concentration according to the color change of the gel.

Embodiment 1

[0020] Preparation method of portable glucose test tube: 200 μL molybdenum disulfide solution (18 mg / L), 300 μL 3,3',5,5'-tetramethylbenzidine solution (12 mM) and 100 μL glucose oxidase Add the solution (10 mg / mL) to 2 mL of agarose (10 mg / mL) hydrogel solution, mix well at 40 °C, and then transfer 150 μL of the mixed solution to the small groove inside the cap of the centrifuge tube, Place it under natural conditions for 5 minutes to make it solidify into a gel to obtain a glucose test tube and store it at 4°C.

[0021] Detection of glucose in serum: Dilute the serum sample 30 times and add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37 °C for 30 min, turn over the centrifuge tube to make the gel and sample solution After separation, the content of glucose in the serum was detected by the color change of the hydrogel.

Embodiment 2

[0023] Preparation method of portable glucose test tube: 200 μL molybdenum disulfide solution (18 mg / L), 300 μL 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20 mM) and 100 μL of glucose oxidase solution (10 mg / mL) were added to 2 mL of chitosan (10 mg / mL) hydrogel solution, mixed at 40 °C, and then 150 μL of the mixed solution was transferred Put it in the small groove inside the cover of the centrifuge tube, and place it under natural conditions for 5 minutes to make it solidify into a gel, and then obtain the glucose test tube, and store it at 4°C.

[0024] Detection of glucose in serum: Dilute the serum sample 30 times and add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37°C for 40 minutes, turn over the centrifuge tube to separate the gel from the sample , to detect the content of glucose in serum by the color change of the hydrogel.

technical field

[0001] The invention belongs to the technical field of glucose detection, in particular to a portable test tube for detecting glucose. Background technique

[0002] In recent years, due to the influence of many factors such as changes in diet structure, increasingly tense life rhythm and less active and more sedentary lifestyle, the incidence of diabetes worldwide has increased rapidly, making diabetes the third serious threat after tumors and cardiovascular diseases Chronic diseases of human health. The detection of glucose in serum is the main means of clinical examination and monitoring of diabetes. At present, most of the device inventions for detecting glucose are electrochemical sensors (patent number: 01810317.0; utility model patent: 200620110322.2; patent number: 201210116431.5; patent number: 201210189645.5; patent number: 201210416267.X; patent number: 201310087177.5; ). The core of these devices is the preparation of functionalized electrodes, ...

Examples

preparation example Construction

[0015] The preparation method mainly includes the following steps: adding molybdenum disulfide solution, chromogen and glucose oxidase solution into the hydrogel solution, mixing evenly, and then transferring the mixed solution to a small groove inside the cover of the centrifuge tube. Under the condition of standing for 5 minutes, make it solidify into a gel, and obtain the glucose test tube.

[0016] The hydrogel includes agar hydrogel, agarose hydrogel or chitosan hydrogel.

[0017] The developer includes 3,3',5,5'-tetramethylbenzidine, 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt or o- phenylenediamine.

[0018] The detection method is to dilute the glucose sample and add it to the bottom of the test tube, cover the lid and turn it upside down, let the sample solution contact with the gel for a period of time, and then semi-quantitatively detect the glucose concentration according to the color change of the gel.

Embodiment 1

[0020] Preparation method of portable glucose test tube: 200 μL molybdenum disulfide solution (18 mg / L), 300 μL 3,3',5,5'-tetramethylbenzidine solution (12 mM) and 100 μL glucose oxidase Add the solution (10 mg / mL) to 2 mL of agarose (10 mg / mL) hydrogel solution, mix well at 40 °C, and then transfer 150 μL of the mixed solution to the small groove inside the cap of the centrifuge tube, Place it under natural conditions for 5 minutes to make it solidify into a gel to obtain a glucose test tube and store it at 4°C.

[0021] Detection of glucose in serum: Dilute the serum sample 30 times and add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37 °C for 30 min, turn over the centrifuge tube to make the gel and sample solution After separation, the content of glucose in the serum was detected by the color change of the hydrogel.

Embodiment 2

[0023] Preparation method of portable glucose test tube: 200 μL molybdenum disulfide solution (18 mg / L), 300 μL 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20 mM) and 100 μL of glucose oxidase solution (10 mg / mL) were added to 2 mL of chitosan (10 mg / mL) hydrogel solution, mixed at 40 °C, and then 150 μL of the mixed solution was transferred Put it in the small groove inside the cover of the centrifuge tube, and place it under natural conditions for 5 minutes to make it solidify into a gel, and then obtain the glucose test tube, and store it at 4°C.

[0024] Detection of glucose in serum: Dilute the serum sample 30 times and add 500 μL to the bottom of the test tube, cover the lid and turn over the test tube, place it at 37°C for 40 minutes, turn over the centrifuge tube to separate the gel from the sample , to detect the content of glucose in serum by the color change of the hydrogel.

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