Biological organic fertilizer for preventing and controlling soil successive cropping obstacles and preparation method thereof

Abstract

The invention relates to a biological organic fertilizer for preventing and controlling soil successive cropping obstacles and a preparation method thereof. The biological organic fertilizer is prepared by mixing medical stone fungicide and cake dreg leavening in a weight ratio of 1:(1-4), and carrying out secondary fermentation under a sealed piling condition for 24-36 hours, wherein the amount of ACCC10388 viable bacteria in the medical stone fungicide is 4*10<8>-5*10<8> / g; the amount of ACCC10634 and ACCC10626 viable bacteria in the cake dreg leavening is 1*10<9>-2*10<9> / g. The invention further provides a preparation method and application of the biological organic fertilizer. According to the invention, the natural medical stones are combined with excellent organic maters and beneficial microorganism to bring a synergistic effect into play, so that the biological organic fertilizer with a special effect is prepared, and intensive agricultural production is realized.

Description

technical field

[0001] The invention relates to a bio-organic fertilizer for preventing and controlling soil continuous cropping obstacles and a preparation method thereof, belonging to the technical field of agricultural intensive production. Background technique

[0002] In recent years, my country's agricultural industrial structure has undergone tremendous changes, and intensive planting has developed rapidly, forming characteristic planting bases, such as cucumber planting bases, tomato planting bases, onion, ginger, garlic and other open-field vegetable planting bases, and achieved good economic benefits. And social benefits, characteristic planting bases have become the most internationally competitive field in my country's agriculture. The development of intensive agriculture not only greatly meets the needs of improving people's living standards, but also has become an important means to transform traditional agriculture and move towards modern agriculture. However,...

Examples

Embodiment 1

[0046] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation. The fermentation production conditions were as follows: the culture broth was made of 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121°C, 0.1MP preservation Sterilize by pressing for 30 minutes. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for culture according to the inoculation amount of 1%. The fermenter was cultured for 24 hours, the culture (fermentation) temperature was 28-30°C, the rotation speed was 200 rpm, the ventilation rate was 1:1 (volume ratio of air to fermentation broth / min); the live bacteria content of the fermentation broth was 8 ×10 9 pieces / ml.

[0047] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 60L / ton, mix evenly, st...

Embodiment 2

[0053] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation production. The fermentation production conditions were as follows: 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121 ° C, 0.1 MP Keep the pressure for 30 minutes to sterilize. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for cultivation according to the inoculation amount of 2% (v / v); cultivate the fermenter for 24 hours, and cultivate ( Fermentation) The temperature is 28-30°C, the rotation speed is 200 rpm, the ventilation rate is 1:1 (the volume ratio of air to fermentation broth / min); the viable bacteria content of fermentation broth is 1×10 10 pieces / ml.

[0054] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 50L / ton, mix evenly, stack and fe...

technical field

[0001] The invention relates to a bio-organic fertilizer for preventing and controlling soil continuous cropping obstacles and a preparation method thereof, belonging to the technical field of agricultural intensive production. Background technique

[0002] In recent years, my country's agricultural industrial structure has undergone tremendous changes, and intensive planting has developed rapidly, forming characteristic planting bases, such as cucumber planting bases, tomato planting bases, onion, ginger, garlic and other open-field vegetable planting bases, and achieved good economic benefits. And social benefits, characteristic planting bases have become the most internationally competitive field in my country's agriculture. The development of intensive agriculture not only greatly meets the needs of improving people's living standards, but also has become an important means to transform traditional agriculture and move towards modern agriculture. However,...

Examples

Embodiment 1

[0046] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation. The fermentation production conditions were as follows: the culture broth was made of 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121°C, 0.1MP preservation Sterilize by pressing for 30 minutes. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for culture according to the inoculation amount of 1%. The fermenter was cultured for 24 hours, the culture (fermentation) temperature was 28-30°C, the rotation speed was 200 rpm, the ventilation rate was 1:1 (volume ratio of air to fermentation broth / min); the live bacteria content of the fermentation broth was 8 ×10 9 pieces / ml.

[0047] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 60L / ton, mix evenly, st...

Embodiment 2

[0053] (1) Preparation of fermentation broth: Microbial strain ACCC10388 was subjected to liquid fermentation production. The fermentation production conditions were as follows: 5 grams of peptone, 5 mg of manganese sulfate monohydrate, 1000 ml of tap water, natural pH, 121 ° C, 0.1 MP Keep the pressure for 30 minutes to sterilize. After activating the slant of the strain, expand the culture in the shake flask for 24 hours, and then transfer it to the fermenter for cultivation according to the inoculation amount of 2% (v / v); cultivate the fermenter for 24 hours, and cultivate ( Fermentation) The temperature is 28-30°C, the rotation speed is 200 rpm, the ventilation rate is 1:1 (the volume ratio of air to fermentation broth / min); the viable bacteria content of fermentation broth is 1×10 10 pieces / ml.

[0054] (2) Preparation of medical stone bacteria agent: Inoculate the fermentation liquid of ACCC10388 into medical stone powder at an amount of 50L / ton, mix evenly, stack and fe...

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