Preparation method and application of reduced glutathione lipidosome

Abstract

The invention discloses a preparation method and application of reduced glutathione lipidosome, relates to a preparation method and application of lipidosome, and aims at solving the technical problem of low inclusion rate of the lipidosome prepared by the existing method. The method comprises the following steps: 1, weighing soya bean lecithin and fatty acid glycerides, dissolving into absolute ethyl alcohol, and heating in a water bath to prepare an oil phase; 2, weighing reduced glutathione and a surfactant to be dissolved into distilled water, and heating in the water bath to prepare a water phase; 3, mixing the prepared oil phase with the water phase, emulsifying, carrying out vacuum rotary evaporation to remove absolute ethyl alcohol, adding distilled water, pre-cooling after ice-bath, and then carrying out freeze drying to obtain lipidosome powder. The reduced glutathione lipidosome disclosed by the invention is applied to intravenous injection, oral medication, transdermal drug delivery, and a drug delivery system for the lung and eyes. The encapsulation efficiency of the reduced glutathione lipidosome disclosed by the invention is 68.15%+ / -1.53%. The preparation method is applied to the field of preparation of the lipidosome.

Description

technical field

[0001] The invention relates to a preparation method and application of liposome. Background technique

[0002] Reduced glutathione (reduced glutathione, referred to as GSH) is a natural active peptide with important physiological functions. It has strong reducing properties and can scavenge free radicals and peroxides. However, the effective components of the reduced glutathione solution will be decomposed and oxidized after 2.5-3 hours, and the content will change, resulting in a decrease in the stability of the solution.

[0003] The patent titled "Reduced Glutathione Percutaneous Absorption Preparation and Its Preparation Method" uses the bag-like space structure of cyclodextrin to include the sulfhydryl part of reduced glutathione to improve the stability of the preparation, and at the same time Utilizing that the binding ability of cyclodextrin and GSH is lower than that of reduced glutathione and skin, GSH is released from the clathrate according to t...

Examples

specific Embodiment approach 1

[0017] Specific embodiment one: the preparation method of a kind of reduced glutathione liposome of the present embodiment is to carry out according to the following steps:

[0018] 1. Weigh 100 to 200 parts of soybean lecithin and 50 to 200 parts of fatty acid glycerides, and then dissolve them in 10 to 100 parts of absolute ethanol, and heat them in a water bath until the temperature is 50°C to 80°C. Prepare the oil phase;

[0019] 2. Weigh 500 to 1000 parts of reduced glutathione and 10 to 100 parts of surfactant, then dissolve them in 20 to 200 parts of distilled water, heat in a water bath to a temperature of 50 to 80 degrees, Prepare the aqueous phase;

[0020] 3. Mix the oil phase prepared in step 1 with the water phase prepared in step 2, then emulsify at a temperature of 50°C to 80°C for 1h to 2h, remove absolute ethanol by rotary evaporation under reduced pressure, and add 10 to 20 parts Distilled water, pre-cooled in ice bath for 1h to 3h at a temperature of -4°C ...

specific Embodiment approach 2

[0025] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the fatty acid glyceride in step 1 is tripalmitin, trilaurin or monostearin. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 1, the water bath is heated to a temperature of 65°C. Others are the same as in the first or second embodiment.

technical field

[0001] The invention relates to a preparation method and application of liposome. Background technique

[0002] Reduced glutathione (reduced glutathione, referred to as GSH) is a natural active peptide with important physiological functions. It has strong reducing properties and can scavenge free radicals and peroxides. However, the effective components of the reduced glutathione solution will be decomposed and oxidized after 2.5-3 hours, and the content will change, resulting in a decrease in the stability of the solution.

[0003] The patent titled "Reduced Glutathione Percutaneous Absorption Preparation and Its Preparation Method" uses the bag-like space structure of cyclodextrin to include the sulfhydryl part of reduced glutathione to improve the stability of the preparation, and at the same time Utilizing that the binding ability of cyclodextrin and GSH is lower than that of reduced glutathione and skin, GSH is released from the clathrate according to t...

Examples

specific Embodiment approach 1

[0017] Specific embodiment one: the preparation method of a kind of reduced glutathione liposome of the present embodiment is to carry out according to the following steps:

[0018] 1. Weigh 100 to 200 parts of soybean lecithin and 50 to 200 parts of fatty acid glycerides, and then dissolve them in 10 to 100 parts of absolute ethanol, and heat them in a water bath until the temperature is 50°C to 80°C. Prepare the oil phase;

[0019] 2. Weigh 500 to 1000 parts of reduced glutathione and 10 to 100 parts of surfactant, then dissolve them in 20 to 200 parts of distilled water, heat in a water bath to a temperature of 50 to 80 degrees, Prepare the aqueous phase;

[0020] 3. Mix the oil phase prepared in step 1 with the water phase prepared in step 2, then emulsify at a temperature of 50°C to 80°C for 1h to 2h, remove absolute ethanol by rotary evaporation under reduced pressure, and add 10 to 20 parts Distilled water, pre-cooled in ice bath for 1h to 3h at a temperature of -4°C ...

specific Embodiment approach 2

[0025] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the fatty acid glyceride in step 1 is tripalmitin, trilaurin or monostearin. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 1, the water bath is heated to a temperature of 65°C. Others are the same as in the first or second embodiment.

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