Artificial breeding production method of trichogramma japonicum

Abstract

The invention provides an artificial breeding production method of trichogramma japonicum. The method is high in production efficiency, short in breeding cycle and beneficial to large-scale production of the richogramma japonicum. The method comprises the following steps that 1, sticky eggs are produced; 2, the sticky eggs are dissolved into single particles from an oviposition medium with a neutral protease solution with the concentration being 0.2%-0.5% (g / ml); 3, the sticky eggs treated in the step 2 are used for breeding the trichogramma japonicum. According to the method, the trichogramma japonicum bred artificially is used for preventing and treating agricultural insects, and the agricultural insects mainly comprise rice-stem borer, tryporyza incertulas and rice leaf folder. Compared with the prior art, the method has the advantages that armyworms are simple in source and easy and convenient to breed; the method is high in production efficiency and short in breeding period, large-scale breeding is convenient, and large-scale production of the trichogramma japonicum is promoted.

Description

technical field

[0001] The invention belongs to the technical field of biological control of agricultural pests, and relates to a breeding and production method for Trichogramma, in particular to an artificial breeding and production method for Trichogramma rice borer. Background technique

[0002] Trichogramma is the most researched and most widely used insect in biological pest control in the world. At least 12 species have been artificially propagated in large numbers to control some important agricultural and forestry pests. The mass breeding of Trichogramma population is mainly carried out by using intermediate host eggs, and the intermediate host eggs of Trichogramma are relatively successfully utilized are tussah silkworm eggs, castor silkworm eggs, wheat moth eggs and rice moth eggs. Large eggs (tussah silkworm eggs, castor silkworm eggs) are easy to obtain and relatively low in cost, so large-egg bees bred with tussah silkworm eggs such as Trichogramma pine caterpil...

Examples

Embodiment 1

[0061] Embodiment 1 breeding Trichogramma rice borer

[0062] 1) Production of armyworm eggs

[0063] 1.1 For armyworm breeding, the temperature is controlled at 26±1°C and the humidity is about 65%.

[0064] 1.1 Raising larvae: place the soon-to-hatch armyworm eggs on one side of the insect tank, and add an appropriate amount of artificial feed to the other side, and cover with a lid with toilet paper to prevent the larvae from escaping. About 100 larvae per tank;

[0065] 1.2 When the larvae grow to the end of the 3rd instar, they are transferred to the large square box for feeding, and artificial feed is added; the number of larvae in each large square box can be adjusted according to the actual situation. Before pupation, there are about 100 larvae in each large square box. 18 days.

[0066] Before pupation, clean up excess feed and keep the box dry to make it pupate smoothly.

[0067] 1.3 Collect the pupae; about 2 days after the pupation is completed, clean up the ...

Embodiment 2

[0083] Embodiment 2 and the comparison of prior art and cost estimation

[0084] Trichogramma rice borer can parasitize the eggs of corn borer and armyworm, but the eggs of these two insects are in the form of lumps, which are not easy to remove from the egg-laying utensils to make egg cards, which limits the application; the life cycle of diamondback moth is short, and the reproduction Ligao can quickly expand the population in a short period of time and obtain a large number of eggs, but the eggs are small, resulting in a decline in the quality of the bred Trichogramma rice borer. Similarly, diamondback moth eggs are not easy to make egg cards and form standardization; Spodoptera litura The amount of eggs laid is large, and it is easy to make a standard egg card, but the Trichogramma rice borer cannot complete the development after being parasitized. Therefore, screening an alternative host with high production efficiency is an important basis for using Trichogramma as a b...

technical field

[0001] The invention belongs to the technical field of biological control of agricultural pests, and relates to a breeding and production method for Trichogramma, in particular to an artificial breeding and production method for Trichogramma rice borer. Background technique

[0002] Trichogramma is the most researched and most widely used insect in biological pest control in the world. At least 12 species have been artificially propagated in large numbers to control some important agricultural and forestry pests. The mass breeding of Trichogramma population is mainly carried out by using intermediate host eggs, and the intermediate host eggs of Trichogramma are relatively successfully utilized are tussah silkworm eggs, castor silkworm eggs, wheat moth eggs and rice moth eggs. Large eggs (tussah silkworm eggs, castor silkworm eggs) are easy to obtain and relatively low in cost, so large-egg bees bred with tussah silkworm eggs such as Trichogramma pine caterpil...

Examples

Embodiment 1

[0061] Embodiment 1 breeding Trichogramma rice borer

[0062] 1) Production of armyworm eggs

[0063] 1.1 For armyworm breeding, the temperature is controlled at 26±1°C and the humidity is about 65%.

[0064] 1.1 Raising larvae: place the soon-to-hatch armyworm eggs on one side of the insect tank, and add an appropriate amount of artificial feed to the other side, and cover with a lid with toilet paper to prevent the larvae from escaping. About 100 larvae per tank;

[0065] 1.2 When the larvae grow to the end of the 3rd instar, they are transferred to the large square box for feeding, and artificial feed is added; the number of larvae in each large square box can be adjusted according to the actual situation. Before pupation, there are about 100 larvae in each large square box. 18 days.

[0066] Before pupation, clean up excess feed and keep the box dry to make it pupate smoothly.

[0067] 1.3 Collect the pupae; about 2 days after the pupation is completed, clean up the ...

Embodiment 2

[0083] Embodiment 2 and the comparison of prior art and cost estimation

[0084] Trichogramma rice borer can parasitize the eggs of corn borer and armyworm, but the eggs of these two insects are in the form of lumps, which are not easy to remove from the egg-laying utensils to make egg cards, which limits the application; the life cycle of diamondback moth is short, and the reproduction Ligao can quickly expand the population in a short period of time and obtain a large number of eggs, but the eggs are small, resulting in a decline in the quality of the bred Trichogramma rice borer. Similarly, diamondback moth eggs are not easy to make egg cards and form standardization; Spodoptera litura The amount of eggs laid is large, and it is easy to make a standard egg card, but the Trichogramma rice borer cannot complete the development after being parasitized. Therefore, screening an alternative host with high production efficiency is an important basis for using Trichogramma as a b...

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